Effect of nitrogen source on pyrimidine catabolism by Pseudomonas fluorescens

1999 ◽  
Vol 154 (3) ◽  
pp. 221-224 ◽  
Author(s):  
Manuel F. Santiago ◽  
Thomas P. West
Genetics ◽  
2001 ◽  
Vol 158 (3) ◽  
pp. 999-1011
Author(s):  
Zoran Gojković ◽  
Michael P B Sandrini ◽  
Jure Piškur

Abstract β-Alanine synthase (EC 3.5.1.6), which catalyzes the final step of pyrimidine catabolism, has only been characterized in mammals. A Saccharomyces kluyveri pyd3 mutant that is unable to grow on N-carbamyl-β-alanine as the sole nitrogen source and exhibits diminished β-alanine synthase activity was used to clone analogous genes from different eukaryotes. Putative PYD3 sequences from the yeast S. kluyveri, the slime mold Dictyostelium discoideum, and the fruit fly Drosophila melanogaster complemented the pyd3 defect. When the S. kluyveri PYD3 gene was expressed in S. cerevisiae, which has no pyrimidine catabolic pathway, it enabled growth on N-carbamyl-β-alanine as the sole nitrogen source. The D. discoideum and D. melanogaster PYD3 gene products are similar to mammalian β-alanine synthases. In contrast, the S. kluyveri protein is quite different from these and more similar to bacterial N-carbamyl amidohydrolases. All three β-alanine synthases are to some degree related to various aspartate transcarbamylases, which catalyze the second step of the de novo pyrimidine biosynthetic pathway. PYD3 expression in yeast seems to be inducible by dihydrouracil and N-carbamyl-β-alanine, but not by uracil. This work establishes S. kluyveri as a model organism for studying pyrimidine degradation and β-alanine production in eukaryotes.


2012 ◽  
Vol 194 (23) ◽  
pp. 6618-6619 ◽  
Author(s):  
Claudia A. Vilo ◽  
Michael J. Benedik ◽  
Daniel A. Kunz ◽  
Qunfeng Dong

ABSTRACTWe report here the 6.97-Mb draft genome sequence ofPseudomonas fluorescensstrain NCIMB 11764, which is capable of growth on cyanide as the sole nitrogen source. The draft genome sequence allowed the discovery of several genes implicated in enzymatic cyanide turnover and provided additional information contributing to a better understanding of this organism's unique cyanotrophic ability. This is the first sequenced genome of a cyanide-assimilating bacterium.


2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity

Author(s):  
Zaid Raad Abbas ◽  
Aqeel Mohammed Majeed Al-Ezee ◽  
Sawsan H

This study was conducted to explore the ability of Pseudomonas fluorescens and Bacillus cereus to solubilizing a phosphate in soil for enhancing the planting growth and, its relation with soill characterization. The isolates were identified as P.fluorescens and B. cereus using convential analysis and, its phosphate solubilization ability and sidrophore was shown by the clear zone formation on National Botanical Research Institute���s Phosphate medium. Moreover, Pseudomonas fluorescens isolates (n = 9) and three of B. cereus isolated from agricultural area in Baghdad university, Mustansiriyah university and Diyala bridge. Results displayed that bacterial count were varied in soil samples according to their region, and ranging from 30 to 60 *10 2 CFU/g in Baghdad university soil to 10���20 *10 2 CFU/g in Mustansiriyah university soil, the Baghdad soil macronutrient which included: NH4, NO3, P, and K were, 8.42, 20.53, 19.09, 218.73 respectively, While the physio analysis revealed that the mean of pH was 7.3 and EC was 8.63. on the other hand the micronutrient analysis indicated that the soil samples were included Ca, Fe, Mn, Zn and Cu which gave their mean 5025.9, 8.9, 4.9, 0.5 and 1.5 respectevily. Results revealed that all isolated bacteria (9 isolates of P.fluorescens and three isolates of B. cereus gave ahalo zone which mean their ability to be phosphate solubilizing bacteria at 100%. Results revealed that all isolated bacteria were detected a ability to produce high levels from chelating agents (siderophores)) by P.fluorescens and. B cereus at 100%, when appeared ahalo clear zone. Furthermore, the high levels of phosphate solubilization and siderophore production were grouped in bacterial species isolated from Iraqi soils. might be attributed to many soil factors such as soil nutrient status, soil acidity, water content, organic matter and soil enzyme activities.


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