Evaluation of myocardial infarct size in rat heart by pinhole SPECT*1

To identify the core structure of 2-aminoethoxydiphenyl borate (2-APB) responsible for the anti-oxidative and protective effect on the ischemia/reperfusion (I/R)-induced heart injury, various 2-APB analogues were analyzed, and several antioxidant assays were performed. Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Myocardial infarct size was quantified using triphenyl tetrazolium chloride (TTC) staining. The levels of tumor necrosis factor-alpha (TNF-α) and cleaved-caspase-3 protein were evaluated as an indicator for the anti-inflammatory and anti-apoptotic effect, respectively. Our data show that 2-APB, diphenylborinic anhydride (DPBA) and 3-(diphenylphosphino)-1-propylamine (DP3A) all exerted the anti-oxidative activity, but only 2-APB and DPBA can scavenge H2O2. 2-APB and DPBA can potently inhibit hydrogen peroxide (H2O2)- and hypoxanthine/xanthine oxidase (HX/XOD)-induced increases in intracellular H2O2 and H9c2 cell death. 2-APB and DPBA were able to decrease the I/R-induced adult rat cardiomyocytes death, myocardial infarct size, and the levels of malondialdehyde (MDA) and creatine kinase-MB (CK-MB). Our results suggest that the two benzene rings with a boron atom comprise the core structure of 2-APB responsible for the anti-oxidative effect mediated through the reaction with H2O2 and generation of phenolic compounds, which in turn reduced the I/R-induced oxidative stress and injury in the rat heart.

Download Full-text

ω-3 Polyunsaturated Fatty Acid Postconditioning Protects the Isolated Perfused Rat Heart from Ischemia-Reperfusion Injury

Cardiorenal Medicine ◽

10.1159/000487490 ◽

2018 ◽

Vol 8 (3) ◽

pp. 173-182 ◽

Cited By ~ 5

Author(s):

Fu-wei Zhang ◽

Jian Tong ◽

Yu-sheng Yan ◽

Qun-qing Chen ◽

Xiao-ping Zhao

Keyword(s):

Infarct Size ◽

Rat Heart ◽

Myocardial Infarct ◽

Ischemia Reperfusion ◽

Left Ventricular ◽

Mitochondrial Damage ◽

Myocardial Infarct Size ◽

Perfused Rat Heart ◽

Isolated Perfused Rat Heart ◽

Cardioprotective Effects

Aims: This study aimed to evaluate the cardioprotective effects of ω-3 polyunsaturated fatty acids (PUFAs) postconditioning against ischemia-reperfusion (I/R) injury. Methods: Sixty Sprague-Dawley rats were randomly divided into 4 groups (n = 15 for each) and used to generate the Langendorff isolated perfused rat heart model. The sham group received a continuous perfusion of 150 min. The remaining three I/R-treated groups sequentially received a 30-min perfusion, a 30-min cardioplegia, and a 90-min reperfusion. The I/R-ischemic preconditioning (IP) group additionally received three cycles of 20-s reperfusion and 20-s coronary reocclusion preceded the 90 min of reperfusion. The I/R-ω group were perfused with ω-3 PUFAs for 15 min before the 90 min of reperfusion. The myocardial infarct size, the degree of mitochondrial damage, the antioxidant capacity of the myocardium, and the cardiac functions during reperfusion were compared among groups. Results: Compared with the I/R group, the I/R-ω group had significantly reduced myocardial infarct size, reduced levels of lactate dehydrogenase and malondialdehyde, elevated superoxide dismutase level, and elevated rising (+dp/dtmax) and descending (–dp/dtmax) rate of left ventricular pressure. The I/R-ω group had a significantly lower rate of mitochondrial damage in myocardial tissue compared with the I/R and I/R-IP groups. Conclusion: ω-3 PUFA postconditioning possesses good cardioprotective effects and may be developed into a therapeutic strategy for myocardial I/R injury.

Download Full-text

Dietary red palm oil supplementation reduces myocardial infarct size in an isolated perfused rat heart model

Lipids in Health and Disease ◽

10.1186/1476-511x-9-64 ◽

2010 ◽

Vol 9 (1) ◽

pp. 64 ◽

Cited By ~ 22

Author(s):

Dirk J Bester ◽

Krisztina Kupai ◽

Tamas Csont ◽

Gergu Szucs ◽

Csaba Csonka ◽

...

Keyword(s):

Infarct Size ◽

Palm Oil ◽

Rat Heart ◽

Myocardial Infarct ◽

Myocardial Infarct Size ◽

Heart Model ◽

Perfused Rat Heart ◽

Red Palm Oil ◽

Isolated Perfused Rat Heart

Download Full-text

Effect of caspase inhibitors on myocardial infarct size and myocyte DNA fragmentation in the ischemia–reperfused rat heart

Cardiovascular Research ◽

10.1016/s0008-6363(99)00271-0 ◽

2000 ◽

Vol 45 (3) ◽

pp. 642-650 ◽

Cited By ~ 102

Author(s):

T Okamura

Keyword(s):

Infarct Size ◽

Dna Fragmentation ◽

Rat Heart ◽

Myocardial Infarct ◽

Myocardial Infarct Size ◽

Caspase Inhibitors

Download Full-text

Monocationic radiotracer kinetics and myocardial infarct size: a perfused rat heart study

Annals of Nuclear Medicine ◽

10.1007/s12149-008-0155-y ◽

2008 ◽

Vol 22 (7) ◽

pp. 617-627 ◽

Cited By ~ 1

Author(s):

David R. Okada ◽

Zhonglin Liu ◽

Delia Beju ◽

Robert D. Okada ◽

Gerald Johnson

Keyword(s):

Infarct Size ◽

Rat Heart ◽

Myocardial Infarct ◽

Myocardial Infarct Size ◽

Perfused Rat Heart ◽

Heart Study

Download Full-text

Myocardial Infarct Size-Limiting and Anti-Arrhythmic Effects of Mildronate Orotate in the Rat Heart

Cardiovascular Drugs and Therapy ◽

10.1007/s10557-009-6179-2 ◽

2009 ◽

Vol 23 (4) ◽

pp. 281-288 ◽

Cited By ~ 16

Author(s):

Reinis Vilskersts ◽

Edgars Liepinsh ◽

Janis Kuka ◽

Helena Cirule ◽

Maris Veveris ◽

...

Keyword(s):

Infarct Size ◽

Rat Heart ◽

Myocardial Infarct ◽

Myocardial Infarct Size

Download Full-text

Remifentanil Protects Myocardium through Activation of AntiApoptotic Pathways of Survival in Ischemia-Reperfused Rat Heart

Physiological Research ◽

10.33549/physiolres.931772 ◽

2010 ◽

pp. 347-356

Author(s):

H S Kim ◽

J E Cho ◽

S W Hong ◽

S O Kim ◽

J K Shim ◽

...

Keyword(s):

Sarcoplasmic Reticulum ◽

Cytochrome C ◽

Infarct Size ◽

Rat Heart ◽

Myocardial Infarct ◽

Coronary Artery Occlusion ◽

Cardioprotective Effect ◽

Myocardial Infarct Size ◽

Sprague Dawley ◽

Apoptotic Protein

Remifentanil is a commonly used opioid in anesthesia with cardioprotective effect in ischemia-reperfused (I/R) heart. We evaluated the influence of remifentanil on myocardial infarct size and expressions of proteins involved in apoptosis in I/R rat heart following various time protocols of remifentanil administration. Artificially ventilated anesthetized Sprague-Dawley rats were subjected to a 30 min of left anterior descending coronary artery occlusion followed by 2 h of reperfusion. Rats were randomly assigned to one of five groups; Sham, I/R only, remifentanil preconditioning, postconditioning and continuous infusion group. Myocardial infarct size, the phosphorylation of ERK1/2, Bcl2, Bax and cytochrome c and the expression of genes influencing Ca2+ homeostasis were assessed. In remifentanil-administered rat hearts, regardless of the timing and duration of administration, infarct size was consistently reduced compared to I/R only rats. Remifentanil improved expression of ERK 1/2 and anti-apoptotic protein Bcl2, and expression of sarcoplasmic reticulum genes which were significantly reduced in the I/R rats only. Remifentanil reduced expression of pro-apoptotic protein, Bax and cytochrome c. These suggested that remifentanil produced cardioprotective effect by preserving the expression of proteins involved in anti-apoptotic pathways, and the expression of sarcoplasmic reticulum genes in I/R rat heart, regardless of the timing of administration.

Download Full-text