Cytotoxic Effects of 24/R,25-Dihydroxycholecalciferol on the Proliferation of Various Tumor Cell Lines in vitro and Its Antitumor Effects in vivo

Five simian virus 40 (SV40)-hepatocyte cell lines were examined for tumorigenicity and the effect of in vitro passage on the expression of four liver-specific genes (albumin, transferrin, alpha 1-antitrypsin, and phosphoenolpyruvate carboxykinase), two oncogenes (c-Ha-ras and c-raf), and two genes associated with hepatocarcinogenesis (alpha-fetoprotein and placental-type glutathione-S-transferase). At low passage (12 to 22), all five cell lines expressed the four liver-specific genes at levels similar to those in the liver and were not tumorigenic or were weakly tumorigenic. At high passage (33 to 61), the cell lines formed carcinomas, and four out of five cell lines produced primary tumors that metastasized. At least two cell lines produced well-differentiated hepatocellular carcinomas that expressed liver-specific RNAs. Levels of expression of liver-specific genes changed with time in culture. Some of the changes in liver-specific gene expression in the tumor tissue (such as for the phosphoenolpyruvate carboxykinase gene) paralleled those that occurred with in vitro passage, while other changes (such as for the albumin gene) did not parallel those that occurred with in vitro passage. Correlations between enhanced expression of c-Ha-ras and tumorigenic potential and between the process of SV40 immortalization and induced expression of c-raf and glutathione-S-transferase-P were observed. Induction of alpha-fetoprotein was detected with in vitro and in vivo passage only in the CWSV14 cell line and was paralleled by diminished albumin expression. In conclusion, we developed a model system with five SV40-hepatocyte cell lines, tumors induced by them, and tumor cell lines to examine changes in gene expression that accompany the progression from a normal cell to a hepatocellular carcinoma. Because the SV40-hepatocyte cell lines and tumor cell lines remain highly differentiated and vary in the magnitude of expression of specific genes, they can be used to study the molecular mechanisms regulating gene expression, in particular those regulating specific genes associated with differentiation.

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Inhibition of Tumor Cell Proliferation In Vitro by Benzamide Derivatives

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.997.225 ◽

2014 ◽

Vol 997 ◽

pp. 225-228 ◽

Cited By ~ 1

Author(s):

Yan Ling Wu ◽

Li Wen Shen ◽

Yan Ping Ding ◽

Yoshimasa Tanaka ◽

Wen Zhang

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Malignant Tumors ◽

Dependent Manner ◽

Tumor Cell Lines ◽

Lead Compounds ◽

Proliferative Effect ◽

Benzamide Derivatives

Benzamide derivatives have been shown to have antitumor activity in various tumor cell lines in vitro as well as in vivo. In this study, we examined the anti-proliferative effect of four benzamide derivativeson Hela, H7402, and SK-RC-42 tumor cell lines in vitro by means of Real-Time cell assay (RTCA), and found that four benzamide derivatives suppressed proliferation of tumor cells in a time-and dose-dependent manner. The anti-proliferative activity of benzamide derivatives demonstrated that theycould be promising lead compounds for developing therapeutic agents for malignant tumors.

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In Vitro and in Vivo Selection of two Lewis Lung Carcinoma Cell Lines

Tumori Journal ◽

10.1177/030089167906500601 ◽

1979 ◽

Vol 65 (6) ◽

pp. 657-664 ◽

Cited By ~ 15

Author(s):

Ada Sacchi ◽

Anna Corsi ◽

Marco Caputo ◽

Gabriella Zupi

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Lung Metastases ◽

Tumor Cell Lines ◽

Lewis Lung ◽

Selection Of

Two tumor cell lines adapted to grow in vitro were originated from an explant of lung metastases of Lewis lung carcinoma. These lines differ in their malignancy when reinoculated into syngeneic animals; nevertheless, they do not show any difference for their in vitro clonogenic ability. From these lines 2 in vivo sublines of 3LL carcinoma were developed. The TD 50 of the 2 in vivo sublines are different, and both the values obtained are lower than that of the original line. These results are interpreted as a selection of more malignant tumor cell lines.

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In VivoandIn VitroAntitumor Effects of Platycodin D, a Saponin Purified from Platycodi Radix on the H520 Lung Cancer Cell

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2014/478653 ◽

2014 ◽

Vol 2014 ◽

pp. 1-17 ◽

Cited By ~ 9

Author(s):

Jae Chan Park ◽

Young Joon Lee ◽

Hae Yun Choi ◽

Yong Kook Shin ◽

Jong Dae Kim ◽

...

Keyword(s):

Tumor Cell ◽

Nude Mice ◽

Oral Treatment ◽

Antitumor Effects ◽

Cytotoxic Effects ◽

Athymic Nude Mice ◽

Platycodin D ◽

Platycodi Radix

Platycodin D is a major pharmacological constituent of Platycodi radix and has showed various pharmacological activities through oxidative stress defense mechanisms. Here, possible antitumor, anticachexia, and immunomodulatory activities of platycodin D were observed on the H520 tumor cell-bearing athymic nude mice after confirming thein vitrocytotoxicity. Platycodin D was orally administered at dose levels of 200, 100, and 50 mg/kg, once a day for 35 days from 15 days after implantation. The results were compared with gemcitabine 160 mg/kg intraperitoneally treated mice (7-day intervals). Platycodin D showed favorable cytotoxic effects on the H520 cells, and also dose-dependently decreased the tumor volumes and weights with increases of apoptotic cells (caspase-3 and PARP immunopositive cells), iNOS and TNF-αimmunoreactivities, decreases of COX-2 immunoreactivities in tumor masses. Platycodin D also showed dose-dependent immunostimulatory and anticachexia effects. Gemcitabine showed favorable cytotoxity against H520 tumor cell and relatedin vivoantitumor effects but aggravated the cancer related cachexia and immunosuppress in H520 tumor cell-bearing athymic nude mice. Taken together, it is considered that oral treatment of platycodin D has potent antitumor activities on H520 cells through direct cytotoxic effects, increases of apoptosis in tumor cells, and immunostimulatory effects and can be control cancer related cachexia.

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Antitumor, Immunomodulatory and Antiangiogenic Efficacy of Medicinal Mushroom Extract Mixtures in Advanced Colorectal Cancer Animal Model

Molecules ◽

10.3390/molecules25215005 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5005

Author(s):

Boris Jakopovic ◽

Nada Oršolić ◽

Sandra Kraljević Pavelić

Keyword(s):

Colorectal Cancer ◽

Tumor Cell ◽

Cell Lines ◽

Macrophage Polarization ◽

Medicinal Mushroom ◽

Tumor Cell Lines ◽

Antitumor Effects ◽

Medicinal Mushrooms ◽

M1 Macrophage

Due to frequent drug resistance and/or unwanted side-effects during conventional and targeted cancer treatments, development of multi-target therapies is an important research field. Medicinal mushrooms’ isolated specific compounds and mushroom extracts have been already proven as non-toxic multi-target inhibitors of specific oncogenic pathways, as well as potent immunomodulators. However, research on antitumor effects of multiple-species extract mixtures was limited so far. The aim of this study was therefore, a study of medicinal mushroom preparations AGARIKON.1 and AGARIKON PLUS on colorectal cell lines in vitro and colorectal mice model in vivo. We found a significant antiproliferative and pro-apoptotic effect of tested medicinal mushroom preparations on colorectal (HCT-116, SW620) tumor cell lines, while the effect on human fibroblast cell line (WI-38) was proliferative emphasizing a specificity towards tumor cell lines. We further investigated the effect of the medicinal mushroom preparations AGARIKON.1 and AGARIKON PLUS in various combinations with conventional cytostatic drug 5-fluorouracil in the advanced metastatic colorectal cancer mouse model CT26.WT. AGARIKON.1 and AGARIKON PLUS exhibited immunostimulatory and antiangiogenic properties in vivo which resulted in significantly increased survival and reduction in tumor volume. The antitumor effects of AGARIKON.1 and AGARIKON PLUS, with or without 5-fluorouracil, are based on M1 macrophage polarization enhancement, inhibition of M2 and tumor-associated macrophage (TAM) polarization, effects on T helper cell Th1/Th2/Th17 cytokine profiles, direct inhibition of CT26.WT tumor growth, inhibition of vascular endothelial growth factors (VEGF) and metalloproteinases 2 and 9 (MMP-2 and MMP-9) modulation. The administration of AGARIKON.1 and AGARIKON PLUS did not show genotoxic effect. This data provides good basis for an expanded translational study.

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Recombinant human interleukin-4 inhibits growth of some human lung tumor cell lines in vitro and in vivo

Lung Cancer ◽

10.1016/0169-5002(94)90377-8 ◽

1994 ◽

Vol 11 (1-2) ◽

pp. 142

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Human Lung ◽

Lung Tumor ◽

Interleukin 4 ◽

Tumor Cell Lines ◽

Human Lung Tumor ◽

Lung Tumor Cell

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Synthesis and biological activity of (S)-2′-fluorodaunorubicin

Canadian Journal of Chemistry ◽

10.1139/v88-030 ◽

1988 ◽

Vol 66 (1) ◽

pp. 187-190 ◽

Cited By ~ 18

Author(s):

Hans H. Baer ◽

Lisa Siemsen

Keyword(s):

Biological Activity ◽

Antitumor Activity ◽

Tumor Cell ◽

Cell Lines ◽

Title Compound ◽

Free Sugar ◽

Tumor Cell Lines ◽

Murine Leukemia

Methyl 3-amino-2,3,6-trideoxy-2-fluoro-β-L-galactopyranoside was hydrolyzed to the free sugar, (S)-2-fluorodaunosamine hydrochloride, which was converted into the α,β-1,4-di-O-acetyl-N-trifluoroacetyl derivative and thence into the corresponding glycosyl bromide. The latter was condensed with daunomycinone, and the product was deprotected to give the title compound. The fluoroanthracycline displayed significant cytotoxicity against a number of tumor cell lines in vitro. Antitumor activity against L1210 murine leukemia in vivo was lower than that of the parent daunorubicin, but toxicity appeared to be reduced.

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