scholarly journals WS02.7 Initial and chronic MRSA infection in cystic fibrosis

2015 ◽  
Vol 14 ◽  
pp. S4
Author(s):  
D.F. Gilpin ◽  
S. Murdock ◽  
L.R. Hoffman ◽  
E. Vallières ◽  
S. McGrath ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Mrsa Infection

Clinical Risk Factors Associated With Persistent MRSA Infection In US Cystic Fibrosis Patients

2010 ◽  
Author(s):  
Elliott C. Dasenbrook ◽  
Christian A. Merlo ◽  
Noah Lechtzin ◽  
Steven Strausbaugh ◽  
Michael W. Konstan ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cystic Fibrosis ◽  
Clinical Risk Factors ◽  
Factors Associated ◽  
Clinical Risk ◽  
Mrsa Infection

scholarly journals Emergence and Within-Host Genetic Evolution of Methicillin-ResistantStaphylococcus aureusResistant to Linezolid in a Cystic Fibrosis Patient

2018 ◽  
Vol 62 (12) ◽  
Author(s):  
Caroline Rouard ◽  
Fabien Garnier ◽  
Jeremy Leraut ◽  
Margaux Lepainteur ◽  
Lalaina Rahajamananav ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Generation Time ◽  
Strong Increase ◽  
Whole Genome ◽  
Content Type ◽  
Mrsa Infection ◽  
Host Genetic ◽  
Mrsa Strains

ABSTRACTMethicillin-resistantStaphylococcus aureus(MRSA) infection has increased in recent years among cystic fibrosis (CF) patients. Linezolid (LZD) is one of the antistaphylococcal antibiotics widely used in this context. Although LZD resistance is rare, it has been described as often associated with long-term treatments. Thirteen MRSA strains isolated over 5 years from one CF patient were studied for LZD resistance emergence and subjected to whole-genome sequencing (WGS). Resistance emerged after three 15-day LZD therapeutic regimens over 4 months. It was associated with the mutation of G to T at position 2576 (G2576T) in all 5rrlcopies, along with a very high MIC (>256 mg/liter) and a strong increase in the generation time. Resistant strains isolated during the ensuing LZD therapeutic regimens and until 13 months after LZD stopped harbored only 3 or 4 mutatedrrlcopies, associated with lower MICs (8 to 32 mg/liter) and low to moderate generation time increases. Despite these differences, whole-genome sequencing allowed us to determine that all isolates, including the susceptible one isolated before LZD treatment, belonged to the same lineage. In conclusion, LZD resistance can emerge rapidly in CF patients and persist without linezolid selective pressure in colonizing MRSA strains belonging to the same lineage.

scholarly journals Molecular characterisation of methicillin-resistant Staphylococcus aureus from chronically colonised cystic fibrosis paediatric patients in Brazil

2020 ◽  
Vol 148 ◽  
Author(s):  
D. C. S. Rodrigues ◽  
D. F. Lima ◽  
R. W. F. Cohen ◽  
E. A. Marques ◽  
R. S. Leão
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Rapid Decline ◽  
High Genetic Diversity ◽  
Methicillin Resistant ◽  
Specialist Centre ◽  
Mrsa Infection ◽  
Resistance Rates ◽  
Panton Valentine Leukocidin ◽  
Chronic Lung Infection

Abstract Persistent methicillin-resistant Staphylococcus aureus (MRSA) infection in cystic fibrosis (CF) patients has been associated with a more rapid decline in lung function, increased hospitalisation and mortality. The aim of this study was to evaluate the clonal relationships among 116 MRSA isolates from 12 chronically colonised CF pediatric patients over a 6-year period in a Rio de Janeiro CF specialist centre. Isolates were characterised by antimicrobial resistance, SCCmec type, presence of Panton-Valentine Leukocidin (PVL) genes and grouped according to DNA macrorestriction profile by pulsed-field gel electrophoresis (PFGE) and spa gene type. High resistance rates were detected for erythromycin (78%) and ciprofloxacin (50%) and SCCmec IV was the most common type (72.4%). Only 8.6% of isolates were PVL positive. High genetic diversity was evident by PFGE (39 pulsotypes) and of nine that were identified spa types, t002 (53.1%) and t539 (14.8%) were the most prevalent. We conclude that the observed homogeneity of spa types within patients over the study period demonstrates the persistence of such strain lineages throughout the course of chronic lung infection.

scholarly journals Impact of newborn screening for cystic fibrosis on clinical outcomes of pediatric patients: 10 years’ experience in Lodz Voivodship

2021 ◽  
Vol 47 (1) ◽  
Author(s):  
M. Olszowiec-Chlebna ◽  
E. Mospinek ◽  
J. Jerzynska
Keyword(s):  
Cystic Fibrosis ◽  
Life Expectancy ◽  
Newborn Screening ◽  
Clinical Outcomes ◽  
Pediatric Patients ◽  
Study Group ◽  
Optimal Method ◽  
Beneficial Effects ◽  
Mrsa Infection

Abstract Background Cystic Fibrosis newborn screening (CFNBS) is the optimal method to diagnose the disease during the asymptomatic period. The aim of the study was to determine how CFNBS affects long term clinical outcomes. Methods Data from infants who were born in Lodz Voivodship, referred to CF center as a part of CFNBS according to IRT/DNA protocol were compared to the data of children with established CF diagnosis before the start of NBS in Poland (Group CF, n = 52). Results In 37 children (during 151 referred infants) the diagnosis of CF was established due to CF NBS (CF NBS Group, n = 37). The average time of diagnosis was 1.59 month in Group CF NBS and 45.25 months in 52 children from Group CF. Pulmonary exacerbations occurred on average 4.2 times in Group CFNBS and they were hospitalized on average 0.5 times compared to Group CF – respectively 6.77 and 2.14 (p < 0.001). The number of PA infected patients increased between the fifth and eighth year of age (OR = 1.16 (95% CI: 1.04–19) (P = 0.007)) regardless of the study group (P = 0.984). Patients with MRSA infection have a higher risk of PA infections in subsequent years of their life (OR = 1.45 (95% CI: 1.03–2.03) (P = 0.032)). Conclusions CF NBS has beneficial effects primarily on decrease of pulmonary withhope for a longer life expectancy and better and centralised treatment in multidisciplinary CF focused centres.

scholarly journals Cystic fibrosis in South Africa: spectrum of disease and determinants of outcome

2021 ◽  
pp. 00856-2020
Author(s):  
M. Zampoli ◽  
J. Verstraete ◽  
M. Frauendorf ◽  
R. Kassanjee ◽  
L. Workman ◽  
...  
Keyword(s):  
South Africa ◽  
Cystic Fibrosis ◽  
Forced Expiratory Volume ◽  
Cross Sectional ◽  
Middle Income ◽  
Black African ◽  
Lower Socioeconomic ◽  
Low Weight ◽  
Multivariable Regression ◽  
Mrsa Infection

IntroductionLittle is known about cystic fibrosis (CF) in low-middle income settings. This study aimed to describe the spectrum and outcomes of CF in South Africa (SA) from the recently established SA CF registry (SACFR).MethodsDemographic, diagnosis and clinical data was extracted from the SACFR. Cross-sectional univariable and multivariable regression analysis of best forced expiratory volume in 1 s (FEV1; age≥6 years) and nutrition (all ages) in 2018 was conducted to investigate factors associated with severe lung disease (SLD; FEV1 ≤3.0 z-score) and undernutrition.ResultsBy December 2018, ancestry of 447 individuals included in the SACFR was Caucasian (315; 70%), mixed (87; 19%) and black African (41; 9%). Median diagnosis age was 7.6 months (IQR 2.7,37.1). Genotype was p.Phe508del homozygous (220; 49%); p.Phe508del heterozygous (144; 32%) and neither p.Phe508del or unknown Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) variant in 83 (19%); the second most frequent CFTR variant was 3120+1G>A, common in black Africans. Median age of patients in 2018 was 14.7 years (IQR 7.4,24.4). SLD was independently associated with chronic methicillin resistant S.aureus (MRSA) (aOR 16.75; 95% CI 1.74–161.50), undernutrition (aOR 5.20; 95% CI 2.23–12.13) and age (aOR 2.23 per 10-years; 95% CI 1.50–3.31). Undernutrition was associated in univariable analysis with low weight at diagnosis, non-caucasian ancestry, chronic P.aeruginosa infection and lower socioeconomic status.ConclusionInterventions targeting MRSA infection and nutrition are needed to improve CF outcomes in SA. Most people with CF in SA are eligible for highly effective CFTR modulator therapy.

scholarly journals Prevalence of Methicillin-Resistant Staphylococcus Aureus (MRSA) infection in patients with Cystic Fibrosis: a meta-analysis

2019 ◽  
Author(s):  
Sagad Omer Obeid Mohamed ◽  
Almigdad H. M. Ali ◽  
Abazr A. H. Ibrahim ◽  
Mahmoud Elnil ◽  
Almutasim B. E. Elhassan ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Meta Analysis ◽  
Healthcare Providers ◽  
Inclusion Criteria ◽  
Methicillin Resistant ◽  
Pooled Prevalence ◽  
Mrsa Infection ◽  
Meta Analyses

Abstract Background Methicillin-resistant staphylococcus aureus (MRSA) infection is increasingly being reported among patients with cystic fibrosis (CF) and contributes to pulmonary morbidity in CF, with poorer prognosis. The aim of this study was to assess the prevalence of MRSA infection in patients with CF. Methods We conducted this study according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Databases of MEDLINE/PubMed, WHO-Virtual Health Library (VHL), ScienceDirect, Google Scholar and OpenGrey were searched to recruit the relevant articles. Pooled prevalence with the corresponding 95% confidence interval (CI) was calculated using OpenMeta Analyst software, and heterogeneity among studies was estimated using the I2 statistics. Results According to our inclusion criteria, 27 studies included a total of 47,413 patients were analyzed. The pooled prevalence of MRSA in patients with CF was 15.2% (95% CI 9.70%– 20.7%). Subgroup analyses and meta-regression showed that the prevalence of MRSA in patients with CF was significantly associated with different geographical areas (P<0.001), data collection method (P<0.001), sample obtaining source (P<0.001), and study year (P = 0.006). Conclusions prevalence of MRSA infection is increasing in patients with CF. the results of this study could provide a reference for further controlling transmission and the management of patients with CF. Healthcare providers need to be aware of the clinically important association between MRSA infection and CF to ensure effective management.

scholarly journals Impact of Newborn Screening for Cystic Fibrosis on Clinical Outcomes of Pediatric Patients: 10 Years’ Experience in Lodz Voivodship.

2020 ◽  
Author(s):  
Olszowiec-Chlebna Małgorzata ◽  
Mospinek Ewa ◽  
Joanna Jerzyńska
Keyword(s):  
Cystic Fibrosis ◽  
Newborn Screening ◽  
Clinical Outcomes ◽  
Pediatric Patients ◽  
Study Group ◽  
Pulmonary Exacerbation ◽  
Optimal Method ◽  
Beneficial Effects ◽  
Mrsa Infection

Abstract Background:Cystic Fibrosis newborn screening (CFNBS) is the optimal method to diagnose the disease during the asymptomatic period. The aim of the study was to determine how CFNBS affects long term clinical outcomes. Methods:Data from infants who were born in Lodz Voivodship, referred to CF center as a part of CFNBS according to IRT/DNA protocol were compered to the data of children with established CF diagnosis before the start of NBS in Poland (Group CF).Results:In 37 children (during 151 referred infants) the diagnosis of CF was established due to CF NBS (CF NBS Group). The average time of diagnosis was 1,59 month in Group CF NBS and 45,25 months in Group CF. Pulmonary exacerbation occurred on average 4,2 times in Group CFNBS and they were hospitalized on average 0,5 times compared to Group CF – respectively 6,77 and 2,14 (p<0,001). The number of PA infected patients increased between the fifth and eighth year of age (OR = 1,16 (95% CI: 1,04-19) (P = 0,007)) regardless of the study group (P = 0.984). Patients with SA MRSA infection have a higher risk of PA infections in subsequent years of their life (OR = 1.45 (95% CI: 1.03-2.03) (P = 0.032)). Conclusions:CF NBS has beneficial effects primarily on decrease of pulmonary exacerbations with hope for a longer life expectancy in these group.

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