Intervention of Spiral Ligament Fibrocytes in the Metabolic Regulation of the Inner Ear

2008 ◽  
Vol 59 (10) ◽  
pp. 494-499 ◽  
Author(s):  
José Ramón García Berrocal ◽  
Iván Méndez-Benegassi ◽  
Cristina Martín ◽  
Rafael Ramírez Camacho
Keyword(s):  
Inner Ear ◽  
Metabolic Regulation ◽  
Spiral Ligament ◽  
Spiral Ligament Fibrocytes

Immunolocalization of aquaporin CHIP in the guinea pig inner ear

1995 ◽  
Vol 269 (6) ◽  
pp. C1450-C1456 ◽  
Author(s):  
K. M. Stankovic ◽  
J. C. Adams ◽  
D. Brown
Keyword(s):  
Inner Ear ◽  
Basilar Membrane ◽  
Polyclonal Antibodies ◽  
Close Association ◽  
Water Channel ◽  
Normal Function ◽  
Oval Window ◽  
Spiral Ligament ◽  
Bony Labyrinth ◽  
Spiral Ligament Fibrocytes

Aquaporin CHIP (AQP-CHIP) is a water channel protein previously identified in red blood cells and water transporting epithelia. The inner ear is an organ of hearing and balance whose normal function depends critically on maintenance of fluid homeostasis. In this study, AQP-CHIP, or a close homologue, was found in specific cells of the inner ear, as assessed by immunocytochemistry with the use of affinity-purified polyclonal antibodies against AQP-CHIP.AQP-CHIP was predominantly found in fibrocytes in close association with bone, including most of the cells lining the bony labyrinth and in fibrocytes lining the endolymphatic duct and sac. AQP-CHIP-positive cells not directly apposing bone include cells under the basilar membrane, some type III fibrocytes of the spiral ligament, fibrocytes of the spiral limbus, and the trabecular perilymphatic tissue extending from the membranous to the bony labyrinth. AQP-CHIP was also found in the periosteum of the middle ear and cranial bones, as well as in chondrocytes of the oval window and stapes. The distribution of AQP-CHIP in the inner ear suggests that AQP-CHIP may have special significance for maintenance of bone and the basilar membrane, and for function of the spiral ligament.

Developmental changes in cochlear spiral ligament fibrocytes of rat inner ear

2007 ◽  
Vol 58 ◽  
pp. S157
Author(s):  
Reiko Nagashima ◽  
Hideki Mutai ◽  
Tatsuo Matsunaga ◽  
Kiyokazu Ogita
Keyword(s):  
Inner Ear ◽  
Developmental Changes ◽  
Spiral Ligament ◽  
Spiral Ligament Fibrocytes

Identification and characterization of an L-type Cav1.2 channel in spiral ligament fibrocytes of gerbil inner ear

2004 ◽  
Vol 125 (1-2) ◽  
pp. 40-46 ◽  
Author(s):  
Fenghe Liang ◽  
Wei Hu ◽  
Bradley A. Schulte ◽  
Cungui Mao ◽  
Chunyan Qu ◽  
...  
Keyword(s):  
Inner Ear ◽  
Spiral Ligament ◽  
Identification And Characterization ◽  
Spiral Ligament Fibrocytes

Molecular cloning of theCochgene of guinea pig inner ear and its expression analysis in cultured fibrocytes of the spiral ligament

2010 ◽  
Vol 130 (8) ◽  
pp. 868-880
Author(s):  
Lishu Li ◽  
Tetsuo Ikezono ◽  
Kuwon Sekine ◽  
Susumu Shindo ◽  
Tomohiro Matsumura ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Inner Ear ◽  
Molecular Cloning ◽  
Expression Analysis ◽  
Spiral Ligament

A voltage- and Ca2+-dependent big conductance K channel in cochlear spiral ligament fibrocytes

2003 ◽  
Vol 445 (6) ◽  
pp. 683-692 ◽  
Author(s):  
F. Liang ◽  
A. Niedzielski ◽  
B. A. Schulte ◽  
S. S. Spicer ◽  
D. J. Hazen-Martin ◽  
...  
Keyword(s):  
K Channel ◽  
Spiral Ligament ◽  
Spiral Ligament Fibrocytes

Spiral ligament fibrocytes release chemokines in response to otitis media pathogens

2006 ◽  
Vol 126 (6) ◽  
pp. 564-569 ◽  
Author(s):  
Sung-Kyun Moon ◽  
Sung-Kyun Moon ◽  
Raekil Park ◽  
Sung-Kyun Moon ◽  
Raekil Park ◽  
...  
Keyword(s):  
Otitis Media ◽  
Spiral Ligament ◽  
Spiral Ligament Fibrocytes

Influence of carbon dioxide laser irradiation on the morphology and function of guinea pig cochlea

2005 ◽  
Vol 119 (9) ◽  
pp. 684-692 ◽  
Author(s):  
DongDong Ren ◽  
JingWU Sun ◽  
GuangLun Wan ◽  
Feng Yang ◽  
Fang Shen
Keyword(s):  
Nitric Oxide ◽  
Carbon Dioxide ◽  
Guinea Pig ◽  
Inner Ear ◽  
Laser Irradiation ◽  
Carbon Dioxide Laser ◽  
Organ Of Corti ◽  
Spiral Ligament ◽  
Laser Application ◽  
Brainstem Response

Both experimental and clinical studies have demonstrated that carbon dioxide laser is suitable for stapedotomy. The aim of this study was to investigate morphological, electrophysiological and functional changes in the inner ear after irradiation with CO2 laser set with different energy parameters.A cochleostomy in the basal cochlear turn of guinea pig cochleae was performed with CO2 laser of 1, 2 and 3 w, respectively. The cochleae were removed three weeks after laser irradiation. The auditory evoked brainstem response (ABR) was measured before and after laser application and immediately before removal of the cochlea. Immunohistochemical methods were used to examine inducible nitric oxide synthase (iNOS/NOSII) and heat-shock protein 70(Hsp70) concentrations in the cochlea after laser application. The organ of Corti was studied by scanning electron microscopy.Worse hearing loss was observed in animals receiving higher-power CO2 laser. These findings correlated with more intense injury of the cochlear ultrastructure and with positiveexpression of iNOS and Hsp70 in spiral ganglion cells, nerve fibres, supporting cells of the organ of Corti and cells of the spiral ligament.The CO2 laser as a noncontact procedure is shown to be effective and safe if the total amount of energy is kept within the limits applied in this study. Nitric oxide and stressproteins play important roles in the traumatic mechanism of the inner ear, which are related tohearing loss and injury of the ultrastructure of the inner ear.

Localization of Glucocorticoid Receptors in the Murine Inner Ear

2002 ◽  
Vol 111 (12) ◽  
pp. 1133-1138 ◽  
Author(s):  
Toshiki Shimazaki ◽  
Masashi Suzuki ◽  
Issei Ichimiya ◽  
Goro Mogi
Keyword(s):  
Inner Ear ◽  
Glucocorticoid Receptors ◽  
Spiral Ligament ◽  
Supporting Cells ◽  
Cochlear Hair Cells ◽  
Sensory Epithelia ◽  
Vestibular Sensory Epithelia ◽  
Cochlear Supporting Cells ◽  
Spiral Ganglia

We performed an immunohistochemical investigation of the distribution of glucocorticoid receptors (GRs) in the murine inner ear and found that GRs were expressed extensively, but with various degrees of immunoreactivity in different regions. We observed the strongest GR expression in the type III fibrocytes of the spiral ligament. Although the immunoreactivity of the cochlear hair cells and of the vestibular sensory epithelia was weak, the neighboring cochlear supporting cells and the subepithelial regions of the vestibular sensory epithelia were immunostained. Staining for GRs was also positive in the spiral ganglia and vestibular ganglia, as well as in the endolymphatic sac. The role of GRs in the inner ear is discussed.

scholarly journals Experimental Study of Local Inner Ear Gene Therapy for Controlling Autoimmune Sensorineural Hearing Loss

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Chang-qiang Tan ◽  
Xia Gao ◽  
Wen-jun Cai ◽  
Xiao-yun Qian ◽  
Ling Lu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Inner Ear ◽  
Recombinant Adenovirus ◽  
Adenovirus Vector ◽  
Sensorineural Hearing ◽  
Spiral Ligament ◽  
Small Blood Vessel ◽  
Recombinant Adenovirus Vector

This study aimed to investigate the efficacy of gene therapy for treating autoimmune sensorineural hearing loss (ASHL) via local administration of a recombinant adenovirus vector containing the Fas ligand or interleukin IL-10 gene. Guinea pigs were divided into four groups, with different microinjections in the scala tympani. Group A were injected with FasL-EGFP, B with IL-10-EGFP, C with EGFP, and D with artificial perilymph. Seven days later, auditory brain-stem response (ABR) was tested, and the temporal bone was stained and observed by light microscopy. The spiral ligament and basement membrane were observed using transmission electron microscopy. FasL and IL-10 expression were examined using immunofluorescence histochemistry. Immunohistochemical analysis showed that the recombinant adenovirus vector in Groups A, B, and C can transfect the stria vascularis, the spiral ligament, the organ of Corti, the spiral ganglion, the region surrounding the small blood vessel in the modiolus, and the cochlear bone wall. Compared with those in Groups C and D, the ABR wave III mean thresholds were significantly lower and the inner ear immunoinflammatory responses in Groups A and B were significantly alleviated. Inhibition of immunoinflammatory response alleviated immunoinflammatory injury and auditory dysfunction. This technique shows potential as a novel therapy for ASHL.

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