scholarly journals Studies on the flow of zinc, cobalt, copper and manganese along the digestive tract of sheep given fresh perennial ryegrass, or white or red clover

1975 ◽  
Vol 34 (1) ◽  
pp. 73-82 ◽  
Author(s):  
N. D. Grace

1. Sheep fitted with a rumen fistula and either a re-entrant cannula at the proximal duodenum or a re-entrant cannula at the terminal ileum were given twice daily 480–520 g dry matter as fresh perennial ryegrass, or white or red clover. Flows of digesta were corrected to 100% recovery of chromic oxide.2. The quantities (g/24 h) of zinc and cobalt leaving the stomach were significantly greater than those in the food. No significant change was found in the quantities of copper and manganese. Significantly smaller quantities of Co (all three diets) and Zn (all diets except red clover) left the small intestine than those which entered this region. No significant differences in the quantities of Cu and Mn entering and leaving the small intestine were found. Significantly smaller quantities of Zn, Co, Cu and Mn were excreted in the faeces than entered the large intestine.3. From the flow results it was determined that there was a significant net secretion of Zn and Co in the stomach, and a significant net absorption of Zn (except with the red-clover diet) and Co from the small intestine, and of Zn, Co, Cu and Mn from the large intestine.

1970 ◽  
Vol 24 (1) ◽  
pp. 197-204 ◽  
Author(s):  
E. Pfeffer ◽  
A. Thompson ◽  
D. C. Armstrong

1. In each of three experiments, two sheep were given diets consisting of hay, or two parts hay to one part barley or one part hay to two parts barley. Each sheep was equipped with a cannula into the rumen and re-entrant cannulas into the proximal duodenum and the terminal ileum. The rations containing barley were supplemented to adjust the intake of calcium, phosphorus, magnesium, sodium and potassium to a level similar to that in the all-hay ration. Paper impregnated with chromic oxide (Cr2O3) was given twice daily by rumen fistula.2. Amounts of crude ash and the five minerals entering and leaving the small intestine and excreted in the faeces were measured. The amounts passing through the re-entrant cannulas were adjusted to give 100% recovery of chromic oxide. The values were used to calculate the direction and net movements of the elements through the walls of the three main parts of the alimentary tract.3. In all instances there was an extensive net secretion of Na and P between mouth and small intestine, net absorption of K and P from the small intestine and of Na from the large intestine.4. The net movements of Ca and Mg were small and rather variable. In five of the six observations there was a small net secretion of Ca and small net absorption of Mg during passage of the digesta through the reticulo-rurnen, omasurn and abomasurn. Net secretion of Ca and Mg apparently occurred in the small intestine and net absorption of Mg in the large intestine.5. The only between-diet differences were small differences in net movements of Na and K.


1969 ◽  
Vol 23 (2) ◽  
pp. 377-387 ◽  
Author(s):  
J. C. Macrae ◽  
D. G. Armstrong

1. In seven experiments sheep were given diets ranging from all-hay to all-barley, and also a diet comprising one part hay to two parts flaked maize. Each sheep was equipped with a cannula into the rumen and a re-entrant cannula in the proximal duodenum; six of the ten also had a re-entrant cannula in the terminal ileum. Paper impregnated with chromic oxide (Cr2O3) was given twice daily by rumen fistula.2. Amounts of α-linked glucose polymer entering and leaving the small intestine and excreted in the faeces were measured. Some values for a fraction designated non-glucose reducing polymer for cellulose and for gross energy were also obtained. The amounts passing the proximal duodenum and the terminal ileum were adjusted to give 100% recovery of Cr2O3 and the values were used to measure the extent of digestion in various parts of the alimentary tract.3. When rolled or whole barley was given alone or was the major feed constituent the amount of α-linked glucose polymer entering the small intestine was 6.0±0.76% of that ingested (range 2.6–8.1%). The value was significantly lower than that found for the diet of hay and flaked maize (10.4±1.3%, range 8.0–13.6 %).The α-linked glucose polymer which entered the small intestine was almost completely digested there.4. The digestibility of the non-glucose reducing polymer, which included much of the hemicelluloses present, ranged from 51 to 73% and almost all the digestible fraction (93–97%) was digested before the small intestine when hay or predominantly hay diets were given. On high-cereal diets only 71–85% of the digested fraction disappeared before the small intestine and appreciable amounts were digested in the large intestine.5. On the all-hay diet 91% of the digestible cellulose and 67% of the digestible energy were lost before the small intestine, 0 and 21% in the small intestine and 9 and 12% in the large intestine.6. Mean digestibility coefficients determined in sheep fed solely on either whole or rolled barley were: for dry matter 88.1 and 87.9%, for nitrogen 83.5 and 82.1%, for crude fibre 53.7 and 56.6% and for gross energy 87.7 and 88.0%.


1971 ◽  
Vol 26 (2) ◽  
pp. 123-134 ◽  
Author(s):  
D. E. Beever ◽  
D. J. Thomson ◽  
E. Pfeffer ◽  
D. G. Armstrong

1. The effect of drying and ensiling ryegrass on the site of digestion of the energy andcarbohydrate fractions was studied in sheep fitted with rumen cannulas and re-entrant can-nulas in the proximal duodenum and terminal ileum.2. The sheep were given fresh (frozen) grass, dried grass, wilted and unwilted silage pre-pared from herbage harvested from the same sward. The grass diets were offered twice dailyto each animal and paper impregnated with chromium sesquioxide was administered twicedaily into the rumen. Twenty-four hour collections of duodenal and ileal digesta, adjusted togive 100 yo recovery of Cr2O3, were analysed to determine the extent of digestion in the fore-stomachs, the small intestine and the caecum and colon.3. Total digestibility of the gross energy was similar for the fresh grass, dried grass andwilted silage diets (67·4,68·1 and67·5 %)but higher for the unwilted silage (72·0 %, P < 0·01).There was an increased flow of energy into the small intestine when the sheep were given driedgrass and unwilted silage. The proportion of the apparently digested energy lost within thesmall intestine was greater when the dried grass was given (302 yo) than when the fresh grasswas given (23·6 yo).4. Drying or ensiling of wilted material affected digestion neither in the entire alimentarytract nor in the different sections of the tract, of some carbohydrate fractions. About 97 yo ofthe digested water-soluble carbohydrate, over 90 yo of the digested cellulose and over 70 yo ofthe digested hemicellulose were digested before reaching the small intestine. The increasedamount of energy entering the duodenum of the sheep given the dried grass was notaccounted for by changes in the fate of these carbohydrate fractions in the digestive tract. Withunwilted silage, digestibilities of the cellulose and hemicellulose fractions were higher, andlower proportions of the digested carbohydrates were lost before the small intestine.


1982 ◽  
Vol 6 ◽  
pp. 162-163 ◽  
Author(s):  
M. A. Overend ◽  
D. G. Armstrong

It has been shown by Beever, Thomson, Cammell and Harrison (1977) that formaldehyde treatment of silage increased amino acid absorption from the small intestine of sheep by 13% and increased the amount of food protein passing through the rumen undegraded by 66% compared to sheep receiving untreated silage. This paper details the digestion of N and apparent uptake of N, non-ammonia N and amino acid N from the small intestine of Jersey cattle fed a silage made from a second growth, mixed S24 perennial ryegrass/red clover sward which was wilted for 24h and then ensiled either without an additive or with a 50/50 mixture of formic acid and formaldehyde (formalin), each at the rate of 2.2 litres/tonne of grass ensiled. The method of ensiling was by use of an Eberhardt Silopresse (Benedict Agricultural Ltd., London). Each of the three cows fed each diet was equipped with a rumen fistula and re-entrant cannulae into the proximal duodenum and terminal ileum.


1990 ◽  
Vol 70 (3) ◽  
pp. 905-920 ◽  
Author(s):  
L. S. GOLFMAN ◽  
R. J. BOILA

Four Holstein steers with cannulae in the rumen, proximal duodenum (PD) and terminal ileum (TI) were fed diets containing: (1) no added Mo or S; (2) Mo added at 10 mg kg−1; (3) S added at 3.0 g kg−1; and (4) Mo and S added at 10 mg and 3.0 g kg−1, respectively, in a Latin square design. Effects of Mo and S on minerals in the digestive tract of steers were evaluated. The solubility of Cu was lower with Mo (P < 0.01) and S (P < 0.01) at the PD and TI, and with Mo plus S (P < 0.05) at the TI. With Mo more (P = 0.07) Cu tended to be excreted in feces. Effects of Mo: Mn, reduced (P < 0.05) input to stomach region and higher (P < 0.01) absorption distal to PD; Zn, lower (P < 0.01) solubility at TI with less (P < 0.05) absorbed from large intestine (LI); Fe, more (P = 0.06) absorbed from LI with lower (P < 0.01) fecal excretion; P, lower (P < 0.05) input to stomach region with a tendency for less (P = 0.08) absorbed from small intestine (SI); Na, less (P < 0.05) absorbed from LI; K, lower (P < 0.05) apparent digestibility. Effects of S: Mn, slight tendency for higher (P = 0.10) absorption distal to PD; Zn, lower (P < 0.05) solubility at PD; P, less (P < 0.05) absorbed from SI and LI; Mg, tendency for less (P = 0.07) absorbed from stomach region and trend for more (P = 0.08) absorbed distal to PD. Minerals other than Cu were influenced by Mo and S in the digestive tract of cattle. Key words: Cattle, molybdenum, sulfur, digestion, minerals


1968 ◽  
Vol 22 (2) ◽  
pp. 261-280 ◽  
Author(s):  
J. H. Topps ◽  
R. N. B. Kay ◽  
E. D. Goodall

1. The flow of digesta to the abomasum and through the duodenum and terminal ileum was measured over 24 h periods in sheep. Pelleted diets of concentrates, principally composed of barley, and of poor-quality hay were given. The seven Scottish Blackface ewes studied were all fitted with rumen cannulas, and in addition two had simple abomasal cannulas, one a re-entrant abomasal cannula, two re-entrant duodenal cannulas, and two re-entrant ileal cannulas.2. Paper impregnated with chromium sesquioxide was given twice daily by rumen fistula. The amounts of dry matter, starch, cellulose, total nitrogen and energy passing through the abomasum, duodenum and ileum and the amounts excreted in the faeces were measured. The flows of digesta were adjusted to give 100% recovery of chromium sesquioxide and the extent of digestion in various parts of the alimentary tract was calculated using these adjusted values. Concentrations of glucose in the blood and of volatile fatty acids (VFA) in the rumen were also measured.3. For the concentrate diet, 69% of the digestible dry matter disappeared in the stomach (reticulo-rumen, omasum and abomasum), 17% in the smail intestine and 14% in the large intestine. Values for disappearance of digestible energy were 72% in the stomach, 23% in the small intestine and 5% in the large intestine. Of the 298 g starch fed daily only 6–35 g passed through the abomasum or duodenum and only 1–4 g reached the terminal ileum. The cellulose in the diet was poorly digested.4. For the hay diet, 67% of the digestible dry matter disappeared in the stomach, 22% in the small intestine and 11% in the large intestine. Values for disappearance of digestible energy were 81% in the stomach, 7% in the small intestine and 12% in the large intestine. Of the 33 g of starch consumed daily, from 5 to 13 g passed through the abomasum or duodenum. The cellulose in the hay was 59% digestible and virtually all this digestion took place in the stomach.5. The concentration of VFA and the proportion of propionate in the rumen fluid, 2·5 h after feeding, were considerably greater on the concentrate diet than on the hay diet, but diet had little influence on the concentration of blood glucose.6. The importance of the small amount of starch passing to the sheep's small intestine is discussed.


1969 ◽  
Vol 23 (1) ◽  
pp. 15-23 ◽  
Author(s):  
J. C MacRae ◽  
D. G. Armstrong

1. The use of chromic oxide (Cr2O3) as an indigestible marker for studies on intestinal digestion in sheep has been examined. The sheep used were equipped with a cannula into the rumen and a re-entrant cannula in the proximal duodenum; some also had a re-entrant cannula in the terminal ileum. The marker was administered twice daily in the form of Cr2O3-impregnated paper, through the rumen cannula.2. Recovery of Cr2O3 in the faeces, based on 7-day collection periods, was satisfactory, the mean recovery for all seven experiments being 99.6 ± 0.7%. In seventeen 24 h collections of digesta entering the proximal duodenum, the mean recovery of the daily dose of marker was 83.7% (range 63.6-148.4%); in eleven such collections at the terminal ileum the mean recovery was 77.3% (54.0-90.0%).3. Detailed examination of the concentrations of Cr2O3 in dry matter was made with individual samples taken during single 24 h periods for five duodenal and three ileal collection periods. There were always marked variations in these concentrations. It is concluded that use of short collection periods to give mean values for the flow of digesta throughout the 24 h, at particular points along the tract, may lead to large errors.


2019 ◽  
Author(s):  
Mahfud Mahfud ◽  
Ihwan

Excessive hunting and poaching for commercial purpose of Varanus salvator in Indonesia can cause a decline in this animal population. However, the scientific information of this animal especially about the biologic of organ system is rarely reported. Therefore, this case opens up opportunities for researching, which aims to study the anatomy of digestive tract of water monitor macroscopically. This research has been conducted in Biology Laboratory, University of Muhammadiyah Kupang for 5 months from March to August 2016. The digestive organ of this animal that has been preserved in alcohol 70% was obtained before from two males of water monitors. Preservation process: the animal were anesthetized, exsanguinated, and fixated in 4 paraformaldehyde by tissue perfusion method. Observations were performed to the visceral site and morphometrical of digestive tract. The resulted data was analysed descriptively and presented in tables and figures. The digestive tract of water monitor consist of esophagus, stomach, small intestine, large intestine and cloaca. The dimension of each organ is different based on its structures and functions. The esophagus of water monitor connects the mouth cavity and the stomach and also as the entrance of food to the stomach. Water monitor stomach were found in cranial part of abdomen, in left side of liver. The small intestine was longer than stomach and it is a winding muscular tube in abdomen in posterior side of liver. The large intestine consist of colon and cloaca, while cecum was not found. This channel was extend lateromedially in abdomen to cloaca between left and right kidneys. The cloaca was the end of digestive tract which excreted feces and urine. From this research, we can conclude that the digestive tract of water monitor consists of esophagus, stomach, small intestine, and large intestine. It’s difficult to differentiate small intestine and large intestine because there are no cecum.


1985 ◽  
Vol 40 (2) ◽  
pp. 267-277 ◽  
Author(s):  
T. A. Stewart ◽  
I. I. McCullough

ABSTRACTSilage cut twice annually (June and August) from a tetraploid red clover/grass sward and three times annually (May, July and September) from a low nitrogen (N) and high N perennial ryegrass/white clover sward was fed in proportion to dry-matter yield from each cut, over a 10-week period, each winter for 3 years to castrated male cattle of initial live weight 401 kg in year 1 and 425 kg in years 2 and 3. The silages were supplemented with 0, 1, 2 and 3 kg concentrate per head daily.Total dry-matter yield from the red clover/grass sward was similar to that from the perennial ryegrass/white clover sward (high N grass) receiving 360 kg N per ha but the digestibility, particularly of first cut material was much lower. Dry-matter production of the low N grass/white clover sward was 0·73 of high N grass sward and produced silages of similar digestibility and fermentation.Dry-matter intakes by the cattle were higher on the legume-based silages in years when clover made a worthwhile contribution to total yield, but this did not significantly improve utilization or animal performance compared with high N grass silage. Mean daily carcass gain per head on red clover/grass silage was 0·41 kg which was significantly less than the 0·61 kg on white clover/grass silage and 0·59 on high N grass (P < 0·001). Carcass output from red clover/grass silage was 618 kg/ha and 629 kg/ha from white clover/grass, both of which were significantly less than the 863 kg/ha from the high N grass silage (P < 0·001). Dressing proportion was also significantly poorer in animals fed red clover/grass silage compared with the other silage types.


1974 ◽  
Vol 32 (3) ◽  
pp. 479-489 ◽  
Author(s):  
J. H. G. Holmes ◽  
H. S. Bayley ◽  
P. A. Leadbeater ◽  
F. D. Horney

1. Six 45 kg pigs with re-entrant ileal cannulas were used in two 3 × 3 Latin-square design experiments to study the site of absorption of protein and amino acids. Semi-purified diets containing soya-bean meal (SBM), rapeseed meal (RSM) or no protein source (protein-free) were offered at the rate of 1 kg dry matter/d.2. Flow-rates of ileal contents for 24 h collection periods, corrected for recovery of marker, were 3135, 3127 and 1243 ml (SE 390) for SBM, RSM and protein-free diets respectively.3. Amounts of dry matter digested in the small intestine were 730, 669 and 809 g/d for SBM, RSM and protein-free diets respectively, all values being significantly different (P < 0·001).4. Nitrogen intakes were 32·6, 29·9 and 5·9 g/d, and amounts digested in the small intestine were 25·7, 20·2 and 1·6 g/d for SBM, RSM and protein-free diets respectively, all values being significantly different (P < 0·001). Amounts digested in the large intestine were 2·6, 3·7 and 0·7 g/d.5. Total amino acid intakes and amounts collected at the ileum and in the faeces were (g/d): SBM, 177, 24 and 18; RSM, 149, 28 and 22; protein-free 3, 9 and 12. Digestibility in the small intestine was higher for SBM than RSM for seventeen of the eighteen amino acids estimated. Greater quantities of arginine, methionine, cystine and tyrosine were voided in the faeces than passed through the ileal cannulas for pigs receiving the SBM and RSM diets. For those receiving the protein-free diet this was true for each amino acid except proline.6. Significant differences were found between all diets in the concentration of some amino acids in ileal and faecal amino-N, and endogenous protein secretions did not mask the differences between diets.7. Differences in digestibility between SBM and RSM were greater at the ileum than in the faeces. Amino acid fermentation in the large intestine obscured or reduced differences between SBM and RSM.


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