8.3 The Digestion of a Wilted Silage made with and without an Additive

1982 ◽  
Vol 6 ◽  
pp. 162-163 ◽  
Author(s):  
M. A. Overend ◽  
D. G. Armstrong
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Formic Acid ◽  
Perennial Ryegrass ◽  
Terminal Ileum ◽  
Red Clover ◽  
Food Protein ◽  
Jersey Cattle ◽  
Second Growth ◽  
Formaldehyde Treatment

It has been shown by Beever, Thomson, Cammell and Harrison (1977) that formaldehyde treatment of silage increased amino acid absorption from the small intestine of sheep by 13% and increased the amount of food protein passing through the rumen undegraded by 66% compared to sheep receiving untreated silage. This paper details the digestion of N and apparent uptake of N, non-ammonia N and amino acid N from the small intestine of Jersey cattle fed a silage made from a second growth, mixed S24 perennial ryegrass/red clover sward which was wilted for 24h and then ensiled either without an additive or with a 50/50 mixture of formic acid and formaldehyde (formalin), each at the rate of 2.2 litres/tonne of grass ensiled. The method of ensiling was by use of an Eberhardt Silopresse (Benedict Agricultural Ltd., London). Each of the three cows fed each diet was equipped with a rumen fistula and re-entrant cannulae into the proximal duodenum and terminal ileum.

scholarly journals Studies on the flow of zinc, cobalt, copper and manganese along the digestive tract of sheep given fresh perennial ryegrass, or white or red clover

1975 ◽  
Vol 34 (1) ◽  
pp. 73-82 ◽  
Author(s):  
N. D. Grace
Keyword(s):  
Small Intestine ◽  
Digestive Tract ◽  
Large Intestine ◽  
Perennial Ryegrass ◽  
Terminal Ileum ◽  
Dry Matter ◽  
Red Clover ◽  
Chromic Oxide ◽  
Proximal Duodenum ◽  
Cobalt Copper

1. Sheep fitted with a rumen fistula and either a re-entrant cannula at the proximal duodenum or a re-entrant cannula at the terminal ileum were given twice daily 480–520 g dry matter as fresh perennial ryegrass, or white or red clover. Flows of digesta were corrected to 100% recovery of chromic oxide.2. The quantities (g/24 h) of zinc and cobalt leaving the stomach were significantly greater than those in the food. No significant change was found in the quantities of copper and manganese. Significantly smaller quantities of Co (all three diets) and Zn (all diets except red clover) left the small intestine than those which entered this region. No significant differences in the quantities of Cu and Mn entering and leaving the small intestine were found. Significantly smaller quantities of Zn, Co, Cu and Mn were excreted in the faeces than entered the large intestine.3. From the flow results it was determined that there was a significant net secretion of Zn and Co in the stomach, and a significant net absorption of Zn (except with the red-clover diet) and Co from the small intestine, and of Zn, Co, Cu and Mn from the large intestine.

The influence of formaldehyde application at ensiling and of supplementation with urea or maize starch on the utilization by calves of ryegrass and red clover silages

1983 ◽  
Vol 36 (3) ◽  
pp. 371-381 ◽  
Author(s):  
A. G. Kaiser ◽  
D. F. Osbourn ◽  
P. England
Keyword(s):  
Organic Matter ◽  
Weight Gain ◽  
Formic Acid ◽  
Dry Matter ◽  
Nitrogen Retention ◽  
Live Weight ◽  
Red Clover ◽  
Carcass Weight ◽  
Live Weight Gain ◽  
Formaldehyde Treatment

ABSTRACTPrimary growths of perennial ryegrass and red clover were ensiled with formic acid (2 1/t fresh crop), o an equal quantity of formic acid together with formaldehyde (46 or 49 g/kg crude protein in the ryegrass or red clover respectively). The four silages were offered ad libitum to 12 groups, each of five British Friesian steer calves, either alone or with urea or maize starch supplements at 18·4 or 185·2 g/kg total dry-matter intake respectively.Digestible organic matter and digestible energy intake, live-weight gain, carcass weight, nitrogen retention and all digestibility measurements were higher on the ryegrass silages than on the red clover silages. However, dry-matter intake was higher on the red clover silages.Formaldehyde treatment reduced lactic and total acid content, and protein degradation in the silages. It also increased intake, live-weight gain and nitrogen retention on ryegrass but not on red clover, the effect being greater when the urea supplement was given. Digestibility measurements were depressed by formaldehyde treatment, although cellulose digestibility was only depressed in the ryegrass silage.Supplementation with starch depressed silage intake and nitrogen and cellulose digestibility, but increased dry matter, organic matter and energy digestibilities, digestible organic matter and digestible energy intakes, live-weight gain and carcass weight. The positive intake, live-weight gain and carcass weight responses were greater on the silages treated with formic acid, while the digestibility and the live-weight gain responses were greater on the red clover silages. Starch supplementation did not improve nitrogen retention.The different animal production responses to formaldehyde treatment on the ryegrass and red clover silages are discussed.

The influence of the method of preservation of forages on the digestion in dairy cows. 2. Digestion of organic matter, energy and amino acids in forestomachs and intestines.

1975 ◽  
Vol 23 (2) ◽  
pp. 89-103
Author(s):  
S. Tamminga
Keyword(s):  
Amino Acids ◽  
Organic Matter ◽  
Small Intestine ◽  
Amino Acid ◽  
Formic Acid ◽  
Dairy Cows ◽  
Grass Silage ◽  
Matter Energy

2. Two dairy cows with duodenal reentrant and rumen cannulae were given diets containing artificially dried and pelleted grass (GP), grass silage treated with formic acid (GSF), grass silage treated with a mixture of formic acid and formaldehyde (GSFF) or grass silage without additive (GS). DM intake was 14.8 to 16.0 kg/day and about 30% of the N in the diets was from grass pellets or silages. Between 45 and 57% of the apparently digested organic matter and between 26 and 41% of the apparently digested energy disappeared before the intestines. The higher values were found with GSF and GS.Total amino acid N reaching the duodenum was between 104 and 134% of intake, highest with GP and GSFF. The amounts of individual amino acids reaching the small intestine were from 75 to 270% of the amounts ingested. High values (>150%) were found for glycine, lysine, methionine and tyrosine; low values (

The influence of formic acid and formaldehyde additives and type of harvesting machine on the utilization of nitrogen in lucerne silages: 2. Changes in amino-acid composition during ensiling and their influence on nutritive value

1978 ◽  
Vol 91 (3) ◽  
pp. 717-725 ◽  
Author(s):  
T. N. Barry ◽  
D. N. Mundell ◽  
R. J. Wilkins ◽  
D. E. Beever
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Formic Acid ◽  
Glutamic Acid ◽  
Amino Acid Composition ◽  
Aspartic Acid ◽  
Nutritive Value ◽  
Formaldehyde Treatment ◽  
Amino Acid Degradation ◽  
Voluntary Intake

SummaryLucerne was made into ten silages using either a flail or a precision-chop harvester with application of formaldehyde or a range of rates of formic acid during harvesting. Amino-acid analyses were done on the silages and on samples taken from the herbage at ensiling.In excess of 70% of aspartic acid, serine, glutamic acid, tyrosine, threonine, lysine, histidine and arginine were destroyed in untreated silages when a flail harvester was used and in excess of 50% when a precision-chop harvester was used. Glycine, leucine, iso-leucine, valine and methionine showed little change, with net losses or increases of up to 10%, whilst proline, cystine + cysteine and phenylalanine were intermediate between these two categories. Net synthesis of alanine and α and γ amino-butyric acids occurred in the untreated silages. Increasing rates of formic acid addition, and the use of the precision-chop harvester, reduced the loss of the amino acids which were extensively degraded in the untreated silages and minimized the increases in alanine and α and γ amino-butyric acids. Formaldehyde treatment also reduced amino-acid degradation, apart from apparently high losses of lysine, histidine and tyrosine. It was concluded, however, that these losses arose through problems of estimation in formaldehyde-treated silages following HCl hydrolysis.It is suggested that the most probable precursors for alanine and α and γ aminobutyric acids were respectively aspartic acid, threonine and glutamic acid. Voluntary intake and the utilization of silage nitrogen by young sheep were related to the net changes in amino acids involved in decarboxylation reactions, the best measures of this being the interconversions involving the formation of alanine and α and γ aminobutyric acids. Deamination reactions appeared to be of much less importance in limiting silage nutritive value than those involving decarboxylation.

scholarly journals Ileal amino acid digestibilities in pigs of barley-based diets with inclusion of lucerne (Medicago sativa), white clover (Trifolium repens), red clover (Trifolium pratense) or perennial ryegrass (Lolium perenne)

1999 ◽  
Vol 82 (2) ◽  
pp. 139-147 ◽  
Author(s):  
M. Reverter ◽  
T. Lundh ◽  
J. E. Lindberg
Keyword(s):  
Amino Acid ◽  
Medicago Sativa ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
White Clover ◽  
Trifolium Repens ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Growing Pigs ◽  
Ileal Digestibility

Two experiments were performed with post-valve T-cannulated growing pigs, using five animals in each experiment in a change-over design to evaluate the effect of inclusion of four different dried forage meals on ileal crude protein (CP) and amino acid (AA) digestibilities. The control diets (C1 and C2) were barley-based and the experimental diets were formulated by replacing the barley with 100 or 200 g/kg of either lucerne (Medicago sativa) or white clover (Trifolium repens) meal in Expt 1 and red clover (Trifolium pratense) or perennial ryegrass (Lolium perenne) meal in Expt 2. A decrease (P< 0·05) in the apparent ileal digestibility of CP and most of the essential and nonessential AA was found with the inclusion of lucerne, white clover and perennial ryegrass meal in the barley-based diets. When red clover meal was included, only the apparent ileal digestibilities of CP, leucine, phenylalanine, tyrosine and glutamic acid were found to decrease (P< 0·05). The estimated apparent ileal digestibilities of most essential AA in the forage meals were lower than in the barley-based diets. The ileal flow of glucosamine and ornithine was found to increase (P< 0·05) with increasing proportion of fibre in the diet, suggesting an increase in endogenous N secretions and small-intestinal microbial activity. With the minor changes found for ileal essential AA digestibilities with forage meal inclusion in the diet the present data confirm the potential of forage meals as a source of protein in pig diets.

The digestion of untreated and formaldehyde-treated soya-bean and rapeseed meals by cattle fed a basal silage diet

1983 ◽  
Vol 100 (2) ◽  
pp. 329-342 ◽  
Author(s):  
J. A. Rooke ◽  
I. M. Brookes ◽  
D. G. Armstrong
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Soya Bean ◽  
Protein Supplements ◽  
Formaldehyde Treatment ◽  
Bean Meal ◽  
Low Efficiency ◽  
Soya Bean Meal ◽  
In Sacco

SUMMARYThe effect of pretreating soya-bean and rapeseed meals with formaldehyde was studied. Soya-bean and rapeseed meals, both untreated and formaldehyde-treated, were fed to cattle receiving a basal diet of silage in a 4 × 4 latin square. Formaldehyde treatment of both meals significantly reduced apparent N digestibility. All four protein supplements increased the amounts of non-ammonia N and amino acid N entering the small intestine over those obtained when silage was fed alone. Formaldehyde treatment of the meals increased the amounts of non-ammonia N and amino acid N entering the small intestine; this effect was significant, however, for only the formaldehyde treatment of the soya-bean meal. The low efficiency of microbial N synthesis observed when silage was fed alone was increased by the inclusion of both of the soyabean meals in the diet but not by the inclusion of the rapeseed meals. Formaldehyde treatment reduced the apparent degradability of the soya-bean meal N, determined in vivo, from 0·90 for the untreated meal to 0·40 for the formaldehyde-treated meal; similarly the degradability of the untreated rapeseed meal was reduced from 0·77 to 0·41 by the formaldehyde treatment. The in sacco technique gave values for degradability for the protein supplements which ranked them in a similar manner as did the in vivo technique; however, for any one meal the in sacco value for degradability was lower than that determined in vivo.

Effects of formaldehyde treatment of casein and peanut meal supplements on amino acids in digesta and plasma of lambs and sheep

1974 ◽  
Vol 25 (4) ◽  
pp. 583 ◽  
Author(s):  
GJ Faichney
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Dietary Protein ◽  
Plasma Levels ◽  
Peanut Meal ◽  
Protein Diet ◽  
Molar Proportion ◽  
Formaldehyde Treatment ◽  
The Individual

The passage of amino acids into the duodenum and the levels of amino acids in jugular plasma were studied (a) in lambs given a supplement of casein with or without formaldehyde treatment, and (b) in sheep given concentrate diets containing treated or untreated peanut meal at both a low and a high level of dietary protein. When casein in the diet was treated with formaldehyde, losses of individual amino acids due to degradation in the rumen were substantially reduced or prevented so that the amounts reaching the small intestine were substantially increased. This increase in supply of amino acids, which was associated with an increase in the rate of liveweight gain, caused an increase in the plasma levels of most amino acids. However, lysine levels tended to fall, and this resulted in marked reductions in the molar proportion of lysine. When sheep were given formaldehyde-treated peanut meal, the amounts of the individual amino acids reaching the small intestine were greater than when untreated peanut meal was given. When the meal in the higher protein diet was treated, the losses due to degradation were again largely prevented. As amino acid supply to the small intestine increased, the plasma levels of most of the individual amino acids decreased to a minimum and then increased when supply exceeded demand. The molar proportion of lysine decreased when peanut meal in the higher protein diet was treated. An increase in plasma s-N-methyllysine level was characteristic of formaldehyde treatment of dietary protein. Changes in arginine levels tended to be inversely related to changes in total amino acid levels.

The effects of the quantity of formaldehyde applied at ensiling and of urea supplementation at feeding on the utilization of red clover silages by young growing cattle

1981 ◽  
Vol 97 (1) ◽  
pp. 1-11 ◽  
Author(s):  
A. G. Kaiser ◽  
J. C. Tayler ◽  
B. G. Gibbs ◽  
P. England
Keyword(s):  
Weight Gain ◽  
Formic Acid ◽  
Live Weight ◽  
Red Clover ◽  
Carcass Composition ◽  
Limiting Factor ◽  
Feed Conversion ◽  
N Retention ◽  
Live Weight Gain ◽  
Formaldehyde Treatment

SUMMARYSix silages were prepared from a primary growth of red clover using additives of 21 formic acid/t fresh crop together with formaldehyde at 0, 16, 34, 52, 77 or 117 g/kg crude protein (CP) in the crop. These silages were offered to appetite, either alone or with a urea supplement at 19·4 g/kg total dietary dry matter (D.M.), to 60 British Friesian steer calves with an initial mean live weight of 106 kg.Formaldehyde treatment restricted silage fermentation, the effect increasing with level of application. However, butyric acid and 2, 3-butanediol content increased at intermediate levels of application. Protein degradation in the silages was reduced by formaldehyde treatment, as evidenced by a decline in ammonia-N and an increase in insoluble-N content with increasing level of formaldehyde application. The recovery of applied formaldehyde in the silages was low (less than 13%) but increased with level of application.Intake, live-weight gain and feed conversion ratio followed quadratic trends, with formaldehyde having a deleterious effect at high levels of application. The decline in these production measurements was associated with declines in the apparent digestibility of D.M., organic matter (OM), N and energy with increasing level of formaldehyde application, although there was no effect of formaldehyde on cellulose digestibility. Urea supplementation tended to increase intake and live-weight gain at formaldehyde levels greater than 34 g/kg CP, and apart from the expected increase in N digestibility, did not affect the digestibility of other dietary components. As urea supplementation did not overcome the adverse effects of high levels of formaldehyde application on intake, live-weight gain and digestibility, it appears that the supply of rumen-degradable N was not the major limiting factor on these diets.N retention followed a quadratic trend with level of formaldehyde application, increasing at intermediate levels (30–50 g/kg CP) and then declining markedly at the highest level of application. A similar trend was evident when N retention was expressed as a proportion of live-weight gain, suggesting possible formaldehyde effects on carcass composition. However, carcass composition data did not confirm any formaldehyde effect. Urea supplementation did not affect N retention, and N balance data indicated poor utilization of the supplementary urea N.When considering the use of formaldehyde, relative to a formic acid control, in additives applied to red olover at ensiling, these data demonstrate little advantage in favour of formaldehyde and serious disadvantages when large quantities of formaldehyde are applied.

APPARENT DIGESTIBILTY OF AMINO ACIDS AND OTHER NITROGENOUS COMPOUNDS IN THE SMALL INTESTINE OF WETHERS EXPOSED TO A COLD ENVIRONMENT

1989 ◽  
Vol 69 (4) ◽  
pp. 921-929
Author(s):  
J. M. KELLY ◽  
R. J. CHRISTOPHERSON ◽  
R. J. EARLY
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Cold Exposure ◽  
Terminal Ileum ◽  
Ammonia Concentration ◽  
Cold Environment ◽  
Total N ◽  
Nitrogenous Compounds ◽  
Intestinal Digestion

Digestion studies were conducted with eight shorn yearling Suffolk wethers to assess the effects of environmental temperature on intestinal digestion of nonammonia nitrogen (NAN) and amino acid N. The wethers were cannulated in the rumen, abomasum, and terminal ileum and subsequently chronically exposed to either cold (0–2 °C) or warm (21–25 °C) temperatures in a crossover experiment. Sheep were fed a diet of chopped bromegrass hay and digesta flows through the abomasum and terminal ileum were estimated by reference to 103ruthenium-phenanthroline and 51chromium-EDTA. Microbial N contributions to total N in abomasal digesta were estimated with 35sulphur. During cold exposure, mean rumen ammonia concentration was reduced (P < 0.01) by 20%, while net appearance of NAN across the forestomach increased from 2.7 to 7.3 g d−1 (P < 0.05) resulting in a 14% increase in flow of NAN from the abomasum. The latter was associated with a 63% increase in flow of undegraded feed N (P < 0.05) and a nonsignificant decrease in microbial NAN. There was a small increase in flow of most amino acids from the abomasum due to cold exposure, with significant effects for lysine, histidine and tyrosine (P < 0.05). Disappearances of lysine (P < 0.05) and tyrosine (P < 0.005) in the small intestine were increased by the cold environment. Cold exposure increased small intestinal digestion of amino acid N relative to digestible organic matter intake (P < 0.08) suggesting maintenance of amino acid supply relative to that of energy in a cold environment. Key words: Amino acids, digestion, small intestine, sheep, cold environment

Quantitative digestion of fresh herbage by sheep: II. The sites of digestion of some nitrogenous constituents

1974 ◽  
Vol 82 (2) ◽  
pp. 309-319 ◽  
Author(s):  
J. C. MacRae ◽  
M. J. Ulyatt
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Perennial Ryegrass ◽  
Dry Matter ◽  
Ammonia Nitrogen ◽  
The Other ◽  
Once Daily ◽  
Short Rotation ◽  
Available Information

SummarySheep prepared either with a rumen cannula, or with a rumen cannula plus re-entrant cannulae in the duodenum and ileum were fed fresh ‘Ruanui’ perennial ryegrass (R), ‘Manawa’ short rotation ryegrass (M) and white clover (C) at dry-matter intakes ranging from 450 to 1000 g/24 h. Paper impregnated with chromic oxide was given once daily via the rumen fistula as a marker.Amounts of non-ammonia-nitrogen (NAN) entering and leaving the small intestine and nitrogen (N) excreted in the faeces were regressed against the intake of N for each sheep and these equations (all significant P < 0·05) were used to calculate the extent of digestion in the stomach, the small intestine and the large intestine at two levels of OM intake (500 and 800 g daily). Amino acid analyses of feed and digesta samples are also presented together with calculations of the apparent absorptions of individual amino acids from the small intestine.At an intake of 500 g OM amounts of NAN entering the small intestine were significantly greater (P < 0·05) in sheep given M than in sheep given R or C. At an intake of 800 g OM amounts in sheep given M were significantly greater (P < 0·01) than in sheep given R. NAN leaving the small intestine and N excreted in the faeces were similar for all three diets.At an intake of 800 g OM apparent absorptions of NAN from the small intestine of sheep given M (0·47 × N intake + 2·8 g/24 h) and sheep given C (0·51 × N intake – 1·3 g/24 h) were significantly greater (P < 0·01 and P < 0·05 respectively) than of sheep given R (0·41 × N intake – 0·5 g/24 h). As intake of herbage was increased the partition of digestion altered.Only small differences between herbages were found in the amino acid composition (g amino acid/100 g protein) of either duodenal or ileal digesta, but because of the large differences in the flows of NAN, the apparent absorptions of individual amino acids from the small intestine were much higher in sheep given M than in sheep given the other two species.The results are discussed in the light of available information on sites of digestion of herbage diets in sheep.

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