Isolation and characterization of the first microsatellite markers for the southern harvester termite, Microhodotermes viator

2016 ◽  
Vol 106 (4) ◽  
pp. 488-493 ◽  
Author(s):  
N. Muna ◽  
C. O'Ryan
Keyword(s):  
Genetic Structure ◽  
Microsatellite Markers ◽  
Population Level ◽  
Preliminary Test ◽  
Isolation And Characterization ◽  
Scale Population ◽  
Invaluable Tool ◽  
Hardy Weinberg Equilibrium ◽  
Identification And Characterization

AbstractThe southern harvester termite, Microhodotermes viator, is ecologically important due to its nutrient cycling activities and trophic interactions. Additionally, M. viator appears to have very long-lived colonies, which amplifies their effect on the environment. In order to estimate the longevity of a colony it is necessary to understand colony genetic structure. However, intra- and intercolonial genetic structure and levels of relatedness have not yet been examined in this species, likely due to a lack of microsatellite markers that effectively hybridize in this species. Here we describe the identification and characterization of seven microsatellite loci for M. viator, using an enriched approach and a preliminary test of their suitability for studies of fine-scale population genetic structure. Seven polymorphic loci were identified, none of which deviated from Hardy–Weinberg equilibrium. The loci had an average of 5.8 alleles per locus (range: 2–14) and an overall mean heterozygosity of 0.51 ± 0.3. Across all loci, population level pairwise FST values showed significant genetic differentiation. The loci described and preliminary genetic data presented here provide an invaluable tool for future studies of population structure and longevity in M. viator colonies.

scholarly journals Isolation and Characterization of Microsatellite Loci for the Ornamental Plant Primula obconica Hance (Primulaceae)

HortScience ◽  
2010 ◽  
Vol 45 (2) ◽  
pp. 314-315 ◽  
Author(s):  
Hai-fei Yan ◽  
Xue-jun Ge ◽  
Chi-ming Hu ◽  
Gang Hao
Keyword(s):  
Genetic Variation ◽  
Genetic Structure ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Ornamental Plant ◽  
Microsatellite Variation ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Fiasco Protocol

Nine microsatellite loci were isolated from Primula obconica using the FIASCO protocol. We used 30 individuals from three populations for the assessment of microsatellite variation. Seven loci were detected with microsatellite polymorphism. The number of alleles per locus ranged from three to seven. The average observed heterozygosity and expected heterozygosity ranged from 0.167 to 0.6 and from 0.409 to 0.653, respectively. These microsatellite markers will be useful to assess the genetic variation and genetic structure of P. obconica.

scholarly journals Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

HortScience ◽  
2010 ◽  
Vol 45 (4) ◽  
pp. 690-692 ◽  
Author(s):  
Jiang-Chong Wu ◽  
Jing Yang ◽  
Zhi-Jian Gu ◽  
Yan-Ping Zhang
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Moringa Oleifera ◽  
Microsatellite Primers ◽  
Genetic Studies ◽  
Isolation And Characterization ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

scholarly journals Isolation and Characterization of Polymorphic Microsatellite Markers for Two Subterranean Termites

Sociobiology ◽  
2017 ◽  
Vol 64 (3) ◽  
pp. 352 ◽  
Author(s):  
Yu-Lei Dang ◽  
Hong-Gui Zhang ◽  
Yu-Feng Meng ◽  
Min Zhang ◽  
Sha Zhao ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Subterranean Termites ◽  
Polymorphic Markers ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

We isolated 15 and 18 highly polymorphic genomic microsatellite markers from two subterranean termites, Reticulitermes aculabialis and R. labralis, respectively. A total of 53 alleles were detected in 15 microsatellite loci of R. aculabialis, and the alleles were 3.533±1.302 (mean±SD), while the corresponding data of R. labralis were 115 detected alleles in 18 microsatellite loci with 6.389±1.754 alleles. The observed and expected heterozygosity was 0.496±0.236 and 0.564±0.125 in R. aculabialis, and 0.368±0.263 and 0.702±0.115 in R. labralis, respectively. Seven loci were highly polymorphic (PIC>0.5) in R. aculabialis, and 15 loci were highly polymorphic (PIC>0.5) in R. labralis. All loci showed Hardy–Weinberg equilibrium. These polymorphic markers provide useful tools for population genetic and breeding system studies of subterranean termites.

scholarly journals Short Note: Isolation and Characterization of Microsatellite Loci in Castanopsis fissa in Lower Subtropical China

2010 ◽  
Vol 59 (1-6) ◽  
pp. 299-300 ◽  
Author(s):  
Lei Dong ◽  
Zheng-Feng Wang ◽  
Peng Zhu ◽  
Wan-Hui Ye
Keyword(s):  
Genetic Structure ◽  
Microsatellite Markers ◽  
Repetitive Dna ◽  
Microsatellite Loci ◽  
Spatial Genetic Structure ◽  
Short Note ◽  
Fine Scale ◽  
Subtropical China ◽  
Isolation And Characterization

Abstract We report on the development and characterization of ten microsatellite markers from repetitive DNA enriched libraries for Castanopsis fissa from lower subtropical China. The number of alleles ranged from three to thirteen. Observed and expected heterozygosities ranged from 0.265 to 0.818, and 0.270 to 0.873, respectively. These microsatellite markers will be used to study fine-scale spatial genetic structure of C. fissa in 20 ha Dinghushan plot in lower subtropical China.

scholarly journals Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae)

2019 ◽  
Vol 19 (5) ◽  
Author(s):  
C S Silva ◽  
E M G Cordeiro ◽  
A S Corrêa
Keyword(s):  
Population Structure ◽  
Microsatellite Markers ◽  
Null Alleles ◽  
Isolation And Characterization ◽  
Hardy Weinberg Equilibrium ◽  
High Degree ◽  
Soybean Looper ◽  
Lepidoptera Noctuidae ◽  
F Statistics

Abstract We constructed 13 microsatellite markers for Chrysodeixis includens (Walker), a serious crop pest in the Americas. All SSR (Simple Sequence Repeat) markers were polymorphic, with no evidence of linkage disequilibrium between any loci in any population. The total number of alleles per locus ranged from 5 for L3, L9, and L11 to 26 for L6; the mean number of alleles per locus in three populations of C. includens ranged from 2.33 for L3 to 14.67 for L6. Hardy–Weinberg equilibrium (HWE) deviation was not observed in four loci for at least one population (L3, L5, L9, L10). Markers L6, L7, L8, L10, L11, L12, and L13 showed a frequency of null alleles > 0.2 for at least one population. STRUCTURE and F-statistics revealed low population structure among the populations (FST = 0.013) and a high degree of inbreeding (FIS = 0.658). The SSR markers developed here will be useful in future studies on the ecology, demography, host dynamics, and gene flow of C. includens. This information is essential to understand the recent status of C. includens as a key pest in South America.

scholarly journals Isolation and Characterization of 13 Microsatellite Loci from Luculia pinceana (Rubiaceae), a Typical Distylous Species

HortScience ◽  
2010 ◽  
Vol 45 (5) ◽  
pp. 840-841 ◽  
Author(s):  
Wei Zhou ◽  
Hong Wang ◽  
De-Zhu Li ◽  
Jun-Bo Yang ◽  
Wei Zhou
Keyword(s):  
Genetic Structure ◽  
Microsatellite Markers ◽  
Breeding System ◽  
Population Genetic Structure ◽  
Population Genetic ◽  
Allele Number ◽  
Isolation And Characterization ◽  
Capture Method ◽  
Monomorphic Population

Luculia pinceana Hook. (Rubiaceae) is a typical distylous species with dimorphic and long-styled monomorphic populations. Within this study, we developed 13 microsatellite markers from L. pinceana using a modified biotin–streptavidin capture method. Polymorphism of each locus was assessed in 30 individuals from four dimorphic populations and one monomorphic population. The average allele number of these microsatellites was 4.153 per locus ranging from three to seven. The observed and expected heterozygosities were from 0.040 to 0.840 and from 0.571 to 0.769, respectively. Additionally, all 13 identified microsatellite markers were successfully amplified in its related species, L. yunnanensis, 10 of which showed polymorphism. These microsatellite markers could provide a useful tool for further study of the breeding system and the population genetic structure in this species and within other Luculia species.

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