Irradiation dose does not affect male reproductive organ size, sperm storage, and female remating propensity in Ceratitis capitata

2020 ◽  
Vol 111 (1) ◽  
pp. 82-90
Author(s):  
S. Abraham ◽  
V. Díaz ◽  
A. Moyano ◽  
G. Castillo ◽  
J. Rull ◽  
...  
Keyword(s):  
Irradiation Dose ◽  
Ceratitis Capitata ◽  
Mass Rearing ◽  
Reproductive Organ ◽  
Organ Size ◽  
Invasive Pest ◽  
Genetic Sexing ◽  
Male Age ◽  
Female Remating ◽  
Accessory Glands

AbstractThe Mediterranean fruit fly Ceratitis capitata is a globally invasive pest, often controlled with the sterile insect technique (SIT). For the SIT, mass-rearing of the target insect followed by irradiation are imperatives. Sterile males are often less able to inhibit female remating and transfer less number of sperm, and even irradiation could affect male reproductive organs, with consequences for their ability to inhibit female remating. On the other hand, male age could affect their ability to modulate female response after mating. Here, we evaluated the quality of the genetic sexing strain Vienna-8-tsl mass-reared in Bioplanta San Juan, Argentina, under laboratory conditions, with regard to: (i) the ability of sterile males irradiated at 100 or 140 Gy to inhibit female remating, in the same day and at 24 h of first copulation; (ii) the ability of 3, 4 or 5 day-old sterile males to inhibit female remating at 24 h of first copulation, and (iii) the effect of a reduction in irradiation doses on the number of sperm stored by females and reproductive organ size in virgin males. Sterile males were better able than wild males to inhibit female remating in the same day of first copulation and as able as wild males 1 day after first copulation. Male age did not affect their ability to inhibit female receptivity. Number of sperm stored by females, testes size and ectodermal accessory glands size were not affected by male identity, while sterile 100 Gy males had larger mesodermal accessory glands than control lab males. A reduction in irradiation dose does not impact any variable measured, except for percentage of sperm-depleted females: females mated with sterile 100 Gy males had lower probabilities to store sperm. The results showed here are very encouraging for tsl Vienna 8 strain reared in Argentina and are discussed in comparison with previous studies in C. capitata female remating with dissimilar results.

scholarly journals Pupation Substrate Type and Volume Affect Pupation, Quality Parameters and Production Costs of a Reproductive Colony of Ceratitis capitata (Diptera: Tephritidae) VIENNA 8 Genetic Sexing Strain

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 337
Author(s):  
Carlos Pascacio-Villafán ◽  
Luis Quintero-Fong ◽  
Larissa Guillén ◽  
José Pedro Rivera-Ciprian ◽  
Reynaldo Aguilar ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Ceratitis Capitata ◽  
Mass Rearing ◽  
Physicochemical Characteristics ◽  
Production Costs ◽  
Cellulose I ◽  
Coconut Fiber ◽  
Genetic Sexing ◽  
Recycled Paper ◽  
Genetic Sexing Strain

Adequate pupation substrates and substrate volume are critical factors in the mass-rearing of insects for Sterile Insect Technique (SIT) applications. To identify an ideal pupation substrate for a reproductive colony of Ceratitis capitata (Wiedemann) VIENNA 8 genetic sexing strain, we first examined pupation in cellulose from recycled paper (cellulose I), sawdust, fine wheat bran, vermiculite and coconut fiber using a volume of 2.5–12.5 mL of substrate for each 5 mL volume of fly larvae. We found a positive relationship between substrate volume and pupation, with cellulose I generating the highest proportions of pupation and coconut fiber the lowest. Higher proportions of female flies (white pupae) pupated in sawdust. The proportion of female fliers increased as substrate volume rose in sawdust and coconut fiber, whereas it decreased in vermiculite and cellulose. In a second experiment, we tested three types of cellulose differing in physicochemical characteristics (celluloses I, II and III), sawdust, and fine wheat bran using a substrate:larvae ratio of 1:1. The three types of cellulose produced the highest pupation levels. The highest proportions of female fliers were observed in sawdust, and cellulose types III and II. Cellulose III and sawdust at relatively low volumes were more cost-effective to produce one million pupae than other substrates, including fine wheat bran used in a mass-rearing facility in Mexico.

Genetic and cytogenetic analysis of Y-autosome translocations in the Mediterranean fruit fly, Ceratitis capitata

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 264-272 ◽  
Author(s):  
Ph. Kerremans ◽  
E. Gencheva ◽  
G. Franz
Keyword(s):  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Mediterranean Fruit Fly ◽  
Structural Basis ◽  
Mitotic Chromosomes ◽  
Genetic Sexing ◽  
Radiation Induced ◽  
Low Levels ◽  
The Mediterranean

Radiation-induced translocations in the Mediterranean fruit fly, Ceratitis capitata, linking the Y chromosome to either autosome 3 or 4 produced pseudolinkage between sex and the mutations dark pupa (dp) and apricot eye (ap), respectively. The genetic behaviour of six new strains is described and the structural basis of five of them is determined through analysis of polytene and mitotic chromosomes. Five strains exhibited low levels of recombination; however, one strain produced a larger number than expected of aberrant, wild-type females. We provide evidence that this is the consequence of the survival of adjacent-1 segregation products until adulthood.Key words: medfly, mass rearing, genetic sexing, recombination, segregation.

scholarly journals Host Suitability and Fitness-Related Parameters in Coptera haywardi (Hymenoptera: Diapriidae) Reared on Irradiated Ceratitis capitata (Diptera: Tephritidae) Pupae Stemming From the tsl Vienna-8 Genetic Sexing Strain

2020 ◽  
Vol 113 (4) ◽  
pp. 1666-1674 ◽  
Author(s):  
Segundo R Núñez-Campero ◽  
Lorena Suárez ◽  
María Josefina Buonocore Biancheri ◽  
Jorge Cancino ◽  
Fernando Murúa ◽  
...  
Keyword(s):  
Ceratitis Capitata ◽  
Biological Control Agent ◽  
Adult Life ◽  
Mass Rearing ◽  
Control Agent ◽  
Temperature Sensitive ◽  
Full Potential ◽  
Genetic Sexing ◽  
Fitness Parameters ◽  
Genetic Sexing Strain

Abstract Coptera haywardi (Ogloblin) is a pupal endoparasitoid of tephritid flies with great potential as a biological control agent worldwide as it does not attack other Diptera. To reach its full potential, its mass rearing needs to be enhanced lowering costs. Here, we focused on the use of irradiated pupae of Ceratitis capitata (Wiedemann) stemming from the temperature-sensitive lethal (tsl) Vienna-8 genetic sexing strain (= CcVienna-8), which is mass-produced in the San Juan Medfly and Parasitoid Mass Rearing Facility in Argentina. Exposure of 1- to 2-d-old CcVienna-8 pupae irradiated at 90 Gy to 6- to 8-d-old C. haywardi females at a 10:1 host/parasitoid ratio for 24 h turned out to be highly successful for the rearing of this parasitoid. High radiation doses (90–100 Gy) did not adversely influence fitness parameters of C. haywardi offspring F1, namely lifetime reproductive rates, adult life expectancy, and survival time. Demographic parameters in C. haywardi F1 from irradiated CcVienna-8 young pupae were improved compared to those values recorded from parasitoid originated from nonirradiated CcVienna-8 pupae. These findings will help to enhance parasitoid mass rearing for augmentative releases against medfly in Argentinean fruit-producing regions.

Improved stability of genetic sex-separation strains for the Mediterranean fruit fly, Ceratitis capitata

Genome ◽  
1994 ◽  
Vol 37 (1) ◽  
pp. 72-82 ◽  
Author(s):  
G. Franz ◽  
E. Gencheva ◽  
Ph. Kerremans
Keyword(s):  
Ceratitis Capitata ◽  
Recombination Frequency ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Temperature Sensitive ◽  
Genetic Sexing ◽  
Male Recombination ◽  
Comparable Level ◽  
Improved Stability ◽  
Genetic Sexing Strains

In the existing genetic sexing strains for the medfly, Ceratitis capitata, male recombination leads to breakdown of the sexing mechanism under mass rearing conditions. The rate of breakdown depends on the recombination frequency and on the fitness of the recombinants. We have tested two different sexing genes, white pupa and a temperature sensitive lethal, in combination with the translocation T(Y;5)30C. Both sexing strains broke down, although at very different rates. In the case of the white pupa strain, 3.5% recombinants were observed after rearing the strain for 15 generations. The second strain, utilizing white pupa and the temperature sensitive lethal as selectable markers, already reached a comparable level after six generations and was broken down completely in the ninth generation. In these strains the frequency of recombination is high because the breakpoint of T(Y;5)30C and the sexing gene(s) are far apart. To remedy the situation, we have isolated four new translocations with breakpoints located closer to the sexing genes. Mass rearing was simulated for several generations with strains based on these translocations and no breakdown was observed under the conditions used.Key words: medfly, sterile insect technique, genetic sexing, recombination.

Effects of contamination with wild-type males, virgin females or mated females, on the stability of the T:Y(ωp†)30C genetic sexing strain of Ceratitis capitata (Diptera: Tephritidae)

1993 ◽  
Vol 83 (4) ◽  
pp. 599-606
Author(s):  
A.A. Kafu ◽  
E. Busch-Petersen ◽  
R.J. Wood
Keyword(s):  
Y Chromosome ◽  
Ceratitis Capitata ◽  
White Male ◽  
Low Frequency ◽  
Mass Rearing ◽  
Temporary Increase ◽  
Wild Type ◽  
Genetic Sexing ◽  
Fitness Advantage ◽  
Genetic Sexing Strain

AbstractThe T:Y(ωp†)30c genetic sexing strain of Ceratitis capitata (Wiedemann), which is designed to generate males with brown (wild-type) pupae and females with white pupae, was contaminated artificially in a series of population experiments, to investigate the pattern of breakdown of the strain. The frequency of brown female pupae increased whether contamination was with males, unmated females or mated females, but the frequency of white male pupae increased only after male or mated female contamination. Contamination with either sex, mated or unmated, caused a rise in the overall frequency of brown pupae. A fitness advantage of the ωp† gene, when not attached to the Y chromosome, seemed to be indicated. The sex ratio became distorted in favour of females after contamination with females, mated or unmated, but not after male contamination which caused a temporary increase in males. The experiments revealed evidence of a low frequency of natural recombination between ωp† and the translocation breakpoint on the Y chromosome, shown by the appearance of a small number of white males that could not be attributed to the experimental procedure. These findings are shown to be relevant in determining whether wild type contamination has taken place in mass rearing of this strain for sterile male release.

scholarly journals Ceratitis capitata genetic sexing strains: laboratory evaluation of strains from mass-rearing facilities worldwide

2017 ◽  
Vol 164 (3) ◽  
pp. 305-317 ◽  
Author(s):  
A.A. Augustinos ◽  
A. Targovska ◽  
E. Cancio-Martinez ◽  
E. Schorn ◽  
G. Franz ◽  
...  
Keyword(s):  
Ceratitis Capitata ◽  
Mass Rearing ◽  
Laboratory Evaluation ◽  
Genetic Sexing ◽  
Genetic Sexing Strains

scholarly journals Genetic analysis of medfly populations in an area of sterile insect technique applications

2021 ◽  
Author(s):  
Rubén Sancho ◽  
Ana Guillem-Amat ◽  
Elena López-Errasquín ◽  
Lucas Sánchez ◽  
Félix Ortego ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Sterile Insect Technique ◽  
Ceratitis Capitata ◽  
Natural Populations ◽  
Low Frequency ◽  
Fruit Fly ◽  
Wild Populations ◽  
Genetic Sexing ◽  
Environmental Implications ◽  
Genetic Sexing Strains

AbstractThe sterile insect technique (SIT) is widely used in integrated pest management programs for the control of the Mediterranean fruit fly (medfly), Ceratitis capitata. The genetic interactions between the released individuals from the genetic sexing strains (GSS), used for SIT applications worldwide, and wild individuals have not been studied. Under the hypothesis that a number of Vienna GSS individuals released to the field might not be completely sterile and may produce viable offspring, we have analyzed medfly Spanish field populations to evaluate the presence of Vienna strain genetic markers. To this goal, we have used contrasted nuclear and mitochondrial genetic markers, and two novel sets of nuclear polymorphisms with the potential to be markers to discriminate between Vienna and wild individuals. Nuclear Vienna markers located on the 5th chromosome of Vienna males have been found in 2.2% (19 from 875) of the Spanish wild medfly females captured at the area where SIT is applied. In addition, a female-inherited mitochondrial Vienna marker has been found in two from the 19 females showing nuclear Vienna markers. The detection of several of these markers in single individuals represents evidence of the introgression of Vienna strain into natural populations. However, alternative explanations as their presence at low frequency in wild populations in the studied areas cannot be fully discarded. The undesired release of non-fully sterile irradiated GSS individuals into the field and their interactions with wild flies, and the potential environmental implications should be taken into account in the application of the SIT.

Simulation of instability in genetic sexing strains: effects of male recombination in association with other biological parameters

1991 ◽  
Vol 81 (1) ◽  
pp. 11-20 ◽  
Author(s):  
E. Busch-Petersen ◽  
H. Baumgartner
Keyword(s):  
Genetic Recombination ◽  
Mass Rearing ◽  
Biological Parameters ◽  
Genetic Sexing ◽  
Male Recombination ◽  
Rate Of Progression ◽  
Control Programmes ◽  
Males And Females ◽  
Low Levels ◽  
Genetic Sexing Strains

AbstractGenetic systems have been developed in several insect species for separating males and females prior to releasing sterilized males in pest control programmes using the sterile insect technique. The systems generally depend on translocating a readily selectable gene onto the Y chromosome. A potential source of instability in such a system is genetic recombination in the male. Although such recombination was originally thought to be absent in most cyclorrhaphous Diptera, low levels have recently been found. We have developed a computer model which simulates the progression of instability in the presence of male recombination, which can be used to assess the influence of rate of recombination in combination with a range of associated genetic and biological parameters. Male recombination alone or fitness of the Y-linked translocation were found to contribute relatively little to the rate of progression of instability. By contrast reduced fitness or mating competitiveness associated with the selectable gene had a strong effect. The sex ratio and the ratio of carriers to non-carriers of the selectable gene showed patterns characteristic of the parameters modelled. The relevance of such data to the development of suitable strains for genetic sex-separation and the replacement of strains under mass rearing conditions are discussed.

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