scholarly journals Autosomal genetic maps of the Australian Sheep Blowfly, Lucilia cuprina dorsalis R.-D. (Diptera: Calliphoridae), and possible correlations with the linkage maps of Musca domestica L. and Drosophila melanogaster (Mg.)

1981 ◽  
Vol 37 (1) ◽  
pp. 55-69 ◽  
Author(s):  
G. G. Foster ◽  
M. J. Whitten ◽  
C. Konovalov ◽  
J. T. A. Arnold ◽  
G. Maffi
Keyword(s):  
Drosophila Melanogaster ◽  
Musca Domestica ◽  
Genetic Maps ◽  
Lucilia Cuprina ◽  
Linkage Data ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Australian Sheep ◽  
Linkage Relationships

SUMMARYLinkage data and revised maps for 52 autosomal loci in L. cuprina are presented. Examination of the linkage relationships of biochemically and morphologically similar mutations in L. cuprina, Musca domestica L. and Drosophila melanogaster (Mg) suggests that the major linkage groups have survived largely intact during the evolution of the higher Diptera.

Genetic maps of the sheep blowfly Lucilia cuprina: linkage-group correlations with other dipteran genera

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 495-506 ◽  
Author(s):  
G. L. Weller ◽  
G. G. Foster
Keyword(s):  
Drosophila Melanogaster ◽  
Ceratitis Capitata ◽  
Genetic Maps ◽  
Lucilia Cuprina ◽  
Linkage Data ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Relationship Of ◽  
The Relationship ◽  
Linkage Relationships

Linkage data and revised genetic maps for 72 autosomal loci in Lucilia cuprina are presented. Comparison of the linkage relationships of biochemically and morphologically similar mutations in Ceratitis capitata, Drosophila melanogaster, and Musca domestica supports the hypothesis that the major linkage elements have survived relatively intact during evolution of the higher Diptera. The relationship of the linkage groups of the mosquito Aedes aegypti to these species is less clear.Key words: Lucilia, Drosophila, Musca, Ceratitis, linkage maps.

scholarly journals A Consensus Map for Loblolly Pine (Pinus taeda L.). I. Construction and Integration of Individual Linkage Maps From Two Outbred Three-Generation Pedigrees

Genetics ◽  
1999 ◽  
Vol 151 (1) ◽  
pp. 321-330 ◽  
Author(s):  
Mitchell M Sewell ◽  
Bradley K Sherman ◽  
David B Neale
Keyword(s):  
Pinus Taeda ◽  
Loblolly Pine ◽  
Fragment Length Polymorphism ◽  
Random Amplified Polymorphic Dna ◽  
Mapping Function ◽  
Genetic Maps ◽  
Linkage Data ◽  
Linkage Maps ◽  
Consensus Map ◽  
Pinus Taeda L

Abstract A consensus map for loblolly pine (Pinus taeda L.) was constructed from the integration of linkage data from two unrelated three-generation outbred pedigrees. The progeny segregation data from restriction fragment length polymorphism, random amplified polymorphic DNA, and isozyme genetic markers from each pedigree were recoded to reflect the two independent populations of parental meioses, and genetic maps were constructed to represent each parent. The rate of meiotic recombination was significantly greater for males than females, as was the average estimate of genome length for males {1983.7 cM [Kosambi mapping function (K)]} and females [1339.5 cM(K)]. The integration of individual maps allows for the synthesis of genetic information from independent sources onto a single consensus map and facilitates the consolidation of linkage groups to represent the chromosomes (n = 12) of loblolly pine. The resulting consensus map consists of 357 unique molecular markers and covers ∼1300 cM(K).

scholarly journals Development of an SNP Marker Set for Marker-Assisted Backcrossing Using Genotyping-By-Sequencing in Tetraploid Perilla

2021 ◽  
Author(s):  
Yun-Joo Kang ◽  
Bo-Mi Lee ◽  
Jangmi Kim ◽  
Moon Nam ◽  
Myoung-Hee Lee ◽  
...  
Keyword(s):  
Recurrent Selection ◽  
Diploid Species ◽  
Genotyping By Sequencing ◽  
Snp Markers ◽  
Genetic Maps ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Genome Wide ◽  
A Genome ◽  
Marker Assisted Backcrossing

Abstract High-quality molecular markers are essential for marker-assisted selection to accelerate breeding progress. Compared with diploid species, recently diverged polyploid crop species tend to have highly similar homeologous subgenomes, which is expected to limit the development of broadly applicable locus-specific single-nucleotide polymorphism (SNP) assays. Furthermore, it is particularly challenging to make genome-wide marker sets for species that lack a reference genome. Here, we report the development of a genome-wide set of kompetitive allele specific PCR (KASP) markers for marker-assisted recurrent selection (MARS) in the tetraploid minor crop perilla. To find locus-specific SNP markers across the perilla genome, we used genotyping-by-sequencing (GBS) to construct linkage maps of two F2 populations. The two resulting high-resolution linkage maps comprised 2,326 and 2,454 SNP markers that spanned a total genetic distance of 2,133 cM across 16 linkage groups and 2,169 cM across 21 linkage groups, respectively. We then obtained a final genetic map consisting of 22 linkage groups with 1,123 common markers from the two genetic maps. We selected 96 genome-wide markers for MARS and confirmed the accuracy of markers in the two F2 populations using a high-throughput Fluidigm system. We confirmed that 91.8% of the SNP genotyping results from the Fluidigm assay were the same as the results obtained through GBS. These results provide a foundation for marker-assisted backcrossing and the development of new varieties of perilla.

scholarly journals Genetic maps of Saccharum officinarum L. and Saccharum robustum Brandes & Jew. ex grassl

1999 ◽  
Vol 22 (1) ◽  
pp. 125-132 ◽  
Author(s):  
Claudia T. Guimarães ◽  
Rhonda J. Honeycutt ◽  
Gavin R. Sills ◽  
Bruno W.S. Sobral
Keyword(s):  
Dna Markers ◽  
Genetic Model ◽  
Saccharum Officinarum ◽  
Genetic Maps ◽  
Breeding Ecology ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Genetic Linkage Maps ◽  
Arbitrarily Primed Pcr ◽  
Saccharum Robustum

Genetic analysis was performed in a population composed of 100 F1 individuals derived from a cross between a cultivated sugarcane (S. officinarum `LA Purple') and its proposed progenitor species (S. robustum `Mol 5829'). Various types (arbitrarily primed-PCR, RFLPs, and AFLPs) of single-dose DNA markers (SDMs) were used to construct genetic linkage maps for both species. The LA Purple map was composed of 341 SDMs, spanning 74 linkage groups and 1,881 cM, while the Mol 5829 map contained 301 SDMs, spanning 65 linkage groups and 1,189 cM. Transmission genetics in these two species showed incomplete polysomy based on the detection of 15% of SDMs linked in repulsion in LA Purple and 13% of these in Mol 5829. Because of this incomplete polysomy, multiple-dose markers could not be mapped for lack of a genetic model for their segregation. Due to inclusion of RFLP anchor probes, conserved in related species, the resulting maps will serve as useful tools for breeding, ecology, evolution, and molecular biology studies within the Andropogoneae.

scholarly journals High-Quality SNP Linkage Maps Improved QTL Mapping and Genome Assembly in Populus

2020 ◽  
Vol 111 (6) ◽  
pp. 515-530
Author(s):  
Chunfa Tong ◽  
Dan Yao ◽  
Hainan Wu ◽  
Yuhua Chen ◽  
Wenguo Yang ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Genome Assembly ◽  
Perfect Match ◽  
Genetic Maps ◽  
Hybrid Population ◽  
Linkage Maps ◽  
Linkage Groups ◽  
High Quality ◽  
F1 Hybrid ◽  
Wide Range

Abstract With the advances in high-throughput sequencing technologies and the development of new software for extracting single nucleotide polymorphisms (SNPs) across a mapping population, it is possible to construct high-quality genetic maps with thousands of SNPs in outbred forest trees. Two parent-specific linkage maps were constructed with restriction site-associated DNA sequencing data from an F1 hybrid population derived from Populus deltoides and Populus simonii, and applied in QTL mapping and genome assembly. The female P. deltoides map contained 4018 SNPs, which were divided into 19 linkage groups under a wide range of LOD thresholds from 7 to 55. The male P. simonii map showed similar characteristics, consisting of 2097 SNPs, which also belonged to 19 linkage groups under LOD thresholds of 7 to 29. The SNP order of each linkage group was optimal among different ordering results from several available software. Moreover, the linkage maps allowed the detection of 39 QTLs underlying tree height and 47 for diameter at breast height. In addition, the linkage maps improved the anchoring of 689 contigs of P. simonii to chromosomes. The 2 parental genetic maps of Populus are of high quality, especially in terms of SNP data quality, the SNP order within linkage groups, and the perfect match between the number of linkage groups and the karyotype of Populus, as well as the excellent performances in QTL mapping and genome assembly. Both approaches for extracting and ordering SNPs could be applied to other species for constructing high-quality genetic maps.

scholarly journals Scalloped wings Is the Lucilia cuprina Notch Homologue and a Candidate for the Modifier of Fitness and Asymmetry of Diazinon Resistance

Genetics ◽  
1996 ◽  
Vol 143 (3) ◽  
pp. 1321-1337 ◽  
Author(s):  
Andrew G Davies ◽  
Anne Y Game ◽  
Zhenzhong Chen ◽  
Tracey J Williams ◽  
Stephen Goodall ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Cell Adhesion ◽  
Molecular Level ◽  
Polytene Chromosomes ◽  
Resistance Mutation ◽  
Lucilia Cuprina ◽  
N Gene ◽  
Genomic Fragment ◽  
Australian Sheep ◽  
Notch Gene

Abstract The Scalloped wings (Scl) gene of the Australian sheep blowfly, Lucilia cuprina, is shown to be the homologue of the Drosophila melanogaster Notch gene by comparison at the DNA sequence and genetic levels. A L. cuprina genomic fragment, which shows strong identity with the Notch (N) gene at the molecular level, hybridizes to the location of the Scl gene on polytene chromosomes. The two genes are functionally homologous; the dominant and recessive Notch-like phenotypes produced by mutations in the Scl gene allow these alleles to be classed as N-like or Abruptex-like. The Scl gene is under investigation as a candidate for the fitness and asymmetry Modifier (M) of diazinon resistance. We show that M affects the penetrance of wing and bristle phenotypes associated with two Scl alleles in a manner consistent with the M being an allele of Scl. In addition, we report a phenotypic interaction between the diazinon-resistance mutation, Rop-1, and the same alleles of Scl. We propose that the product of Rop-1, an esterase, may be involved in cell adhesion in developmental processes involving the Scl gene product.

Molecular genetic linkage maps for allotetraploid Leymus wildryes (Gramineae: Triticeae)

Genome ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 627-646 ◽  
Author(s):  
Xiaolei Wu ◽  
Steven R Larson ◽  
Zanmin Hu ◽  
Antonio J Palazzo ◽  
Thomas A Jones ◽  
...  
Keyword(s):  
Molecular Genetic ◽  
Perennial Grass ◽  
Perennial Grasses ◽  
Genetic Maps ◽  
Aflp Markers ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Genetic Linkage Maps ◽  
Leymus Triticoides ◽  
Leymus Cinereus

Molecular genetic maps were constructed for two full-sib populations, TTC1 and TTC2, derived from two Leymus triticoides × Leymus cinereus hybrids and one common Leymus triticoides tester. Informative DNA markers were detected using 21 EcoRI–MseI and 17 PstI–MseI AFLP primer combinations, 36 anchored SSR or STS primer pairs, and 9 anchored RFLP probes. The 164-sib TTC1 map includes 1069 AFLP markers and 38 anchor loci in 14 linkage groups spanning 2001 cM. The 170-sib TTC2 map contains 1002 AFLP markers and 36 anchor loci in 14 linkage groups spanning 2066 cM. Some 488 homologous AFLP loci and 24 anchor markers detected in both populations showed similar map order. Thus, 1583 AFLP markers and 50 anchor loci were mapped into 14 linkage groups, which evidently correspond to the 14 chromosomes of allotetraploid Leymus (2n = 4x = 28). Synteny of two or more anchor markers from each of the seven homoeologous wheat and barley chromosomes was detected for 12 of the 14 Leymus linkage groups. Moreover, two distinct sets of genome-specific STS markers were identified in these allotetraploid Leymus species. These Leymus genetic maps and populations will provide a useful system to evaluate the inheritance of functionally important traits of two divergent perennial grass species.Key words: AFLP, perennial grasses, RFLP, STS, SSR.

Construction of three linkage maps in bread wheat, Triticum aestivum

2001 ◽  
Vol 52 (12) ◽  
pp. 1089 ◽  
Author(s):  
K. J. Chalmers ◽  
A. W. Campbell ◽  
J. Kretschmer ◽  
A. Karakousis ◽  
P. H. Henschke ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Doubled Haploid ◽  
Wheat Breeding ◽  
Genetic Maps ◽  
Aflp Markers ◽  
Linkage Maps ◽  
Linkage Groups ◽  
D Genome ◽  
Doubled Haploid Lines ◽  
Wide Range

Genetic maps were compiled from the analysis of 160–180 doubled haploid lines derived from 3 crosses: Cranbrook Halberd, CD87 Katepwa, and Sunco Tasman. The parental wheat lines covered a wide range of the germplasm used in Australian wheat breeding. The linkage maps were constructed with RFLP, AFLP, microsatellite markers, known genes, and proteins. The numbers of markers placed on each map were 902 for Cranbrook Halberd, 505 for CD87 Katepwa, and 355 for Sunco Tasman. Most of the expected linkage groups could be determined, but 10–20% of markers could not be assigned to a specific linkage group. Homologous chromosomes could be aligned between the populations described here and linkage groups reported in the literature, based around the RFLP, protein, and microsatellite markers. For most chromosomes, colinearity of markers was found for the maps reported here and those recorded on published physical maps of wheat. AFLP markers proved to be effective in filling gaps in the maps. In addition, it was found that many AFLP markers defined specific genetic loci in wheat across all 3 populations. The quality of the maps and the density of markers differs for each population. Some chromosomes, particularly D genome chromosomes, are poorly covered. There was also evidence of segregation distortion in some regions, and the distribution of recombination events was uneven, with substantial numbers of doubled haploid lines in each population displaying one or more parental chromosomes. These features will affect the reliability of the maps in localising loci controlling some traits, particularly complex quantitative traits and traits of low heritability. The parents used to develop the mapping populations were selected based on their quality characteristics and the maps provide a basis for the analysis of the genetic control of components of processing quality. However, the parents also differ in resistance to several important diseases, in a range of physiological traits, and in tolerance to some abiotic stresses.

scholarly journals Development, Linkage Mapping, and Use of Microsatellites in Bermudagrass

2010 ◽  
Vol 135 (6) ◽  
pp. 511-520 ◽  
Author(s):  
Karen R. Harris-Shultz ◽  
Brian M. Schwartz ◽  
Wayne W. Hanna ◽  
Jeff A. Brady
Keyword(s):  
Ssr Markers ◽  
Expressed Sequence Tags ◽  
Linkage Mapping ◽  
Genetic Maps ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Marker Data ◽  
Genetic Linkage Maps ◽  
Expressed Sequence ◽  
Simple Sequence

Genetic linkage maps of bermudagrass (Cynodon spp.) species using 118 triploid individuals derived from a cross of T89 [C. dactylon (2n = 4x = 36)] and T574 [C. transvaalensis (2n = 2x = 18)] were enriched with expressed sequence tags-derived simple sequence repeat (EST-SSR) markers. Primers were developed from 53 ESTs containing SSRs producing 75 segregating markers from which 28 could be mapped to the T89 and T574 genetic maps. With the addition of previously generated marker data, 26 T89 linkage groups and eight T574 linkage groups were formed using a log-of-odds (LOD) value of 4.0. The T89 and T574 linkage maps spanned 1055 cM and 311.1 cM and include 125 and 36 single-dose amplified fragments (SDAFs), respectively. Many of the SDAFs displayed disomic segregation and thus T89 may be a segmental allotetraploid or an allotetraploid. The additional EST-SSR markers add value to the maps by increasing marker density and provide markers that can be easily transferred to other bermudagrass populations. Furthermore, EST-SSRs can be immediately used to assess genetic diversity, identify non-mutated cultivars of bermudagrass, confirm pedigrees, and differentiate contaminants from cultivars derived from ‘Tifgreen’.

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