Use of ultrasonography to estimate cistern size and milk storage at different milking intervals in the udder of dairy cows

2003 ◽  
Vol 70 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Moez Ayadi ◽  
Gerardo Caja ◽  
Xavier Such ◽  
Christopher H Knight
Keyword(s):  
Dairy Cows ◽  
Blocking Agent ◽  
Non Invasive ◽  
Milk Volume ◽  
Invasive Method ◽  
Highly Correlated ◽  
Milk Storage ◽  
Transducer Probe ◽  
Storage Characteristics

Four lactating Holstein cows (average milk yield: 20±3 l/d) were used to develop and validate a method for estimating the size of udder cisterns (Sinus lactiferi) using ultrasonography. A sectorial transducer probe of 5 MHz, placed in contact with the teat in a parallel cranial position, was used to obtain vertical scans of the udder in two perpendicular planes with the teat canal axis as reference. Udder scans for each udder quarter were taken randomly at intervals of 4, 8, 12, 16, 20 and 24 h after milking. Glandular parenchyma (echogenic) and lumen of the cisterns full of milk (anechogenic) were evident in the scans, the calculated area of the anechogenic portion being defined as cistern area. Cistern areas measured in perpendicular scans were highly correlated. Immediately after each measurement, cisternal milk was removed from each quarter using a teat cannula after i.v. injection of an oxytocin-receptor blocking agent. Alveolar milk from each quarter was then obtained by machine milking after i.m. injection of oxytocin. Cistern area and cisternal milk volume increased with length of milking interval showing a curvilinear pattern with a plateau after 16 h. Correlations between cistern area and cisternal milk volume were positive and significant (P<0·001) at all intervals but showed the highest values with the smallest residual standard deviations at 8 h (r=0·88) and 12 h (r=0·84). Since 8 h has previously been identified as a suitable time at which to determine cisternal milk volume for the purposes of defining suitability for different milking strategies, we conclude that ultrasonography provides a satisfactory, non-invasive method for determination of milk storage characteristics in dairy cows.

scholarly journals FREQUENCY OF DETERMINATION OF PARENCHYMAL ORGANS DISTURBANCES DURING ULTRASOUND EXAMINATION OF REPRODUCTIVE TRACT

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Strahinja ĆIBIĆ ◽  
Miloš PAVLOVIĆ
Keyword(s):  
Genital Tract ◽  
Reproductive Tract ◽  
Ultrasound Examination ◽  
Non Invasive ◽  
Follicular Cyst ◽  
Pathological Conditions ◽  
Ultrasound Diagnostics ◽  
Liver And Pancreas ◽  
Invasive Method

Ultrasound Real time 2D diagnostics, being an available and non-invasive method, issuccessfully used in human medicine, veterinary medicine and biology to diagnose bothphysiological and pathological conditions. In the case of small animals, the ultrasound is often usedto diagnose gravidity, number, size and vitality of fetuses as well as to diagnose pathologicalconditions present in the genital tract, and is also used to examine testicles and prostate of maleanimals. Today's advanced use of the ultrasound diagnostics provides us with an opportunity todetect numerous pathological conditions of reproductive tract of both female and male animals, andthose conditions directly affect health status of other internal organs. The aim of this study is todiagnose primary genital tract disorders using the ultrasound diagnostics, as well as proving itsconnection to the changes in parenchymal organs. The ultrasound examination was performed onthe total of 12 dogs, 6 male and 6 female dogs. Ovaries, uterus and the organs in the abdominal areawere examined using a transducer ranging from 5 to 8 MHz. A transducer of 7.5 MHz was used toexamine prostates and testicles. The patients were in dorsal position. Three out of six examined maledogs exhibited changes on their prostates, and the remaining one had a tumor on the testicles. Cystitisand hyperplasia of prostate were also found upon the examination of these dogs. One out of sixexamined female dogs, had cystic endometrial hyperplasia, three of them had pyometra, and one ofthem was diagnosed with an follicular cyst. In addition to these pathological conditions, changeswere noticed in the liver and pancreas. Along with the detection of primary changes in the genitaltract of both male and female animals, the ultrasound diagnostics can be used to discover consequentchanges in parenchymal organs.

scholarly journals Visual Scale as a Non-Invasive Method for Evaluation of Risk and Severity of Coronary Atherosclerosis

Kardiologiia ◽  
2021 ◽  
Vol 61 (4) ◽  
pp. 46-52
Author(s):  
M. V. Zhatkina ◽  
N. E. Gavrilova ◽  
V. A. Metelskaya ◽  
E. B. Yarovaya ◽  
B. A. Rudenko ◽  
...  
Keyword(s):  
Coronary Atherosclerosis ◽  
Roc Analysis ◽  
Noninvasive Diagnosis ◽  
Predictive Values ◽  
Non Invasive ◽  
Visual Scale ◽  
Invasive Method ◽  
Ultrasound Parameters ◽  
Qualitative Characteristics

Aim      To evaluate quantitative and qualitative characteristics of atherosclerotic plaques (ASP) in carotid arteries (CA) and femoral arteries (FA) and to use these data for developing a visual scale (VS) for noninvasive diagnosis and determination of severity of coronary atherosclerosis.Material and methods  This study included 216 patients (115 men and 101 women) aged 24 to 87 years (mean age, 61.5±10.73 years). All patients underwent coronary angiography (CAG) for detecting and determining severity of CA atherosclerosis and duplex scanning (DS) for detecting atherosclerosis of CA and FA.Results Analysis of ultrasound parameters of ASP in CA and FA showed that the maximal ASP height, moderate stenosis and maximal stenosis of the arterial bed had higher predictive values than other ultrasound parameters. These parameters were used for forming diagnostic complexes, on the basis of which two individual VSs for CA and FA were developed. Based on the high prognostic value of both scales, they were combined into one that was named VSCOMB. A ROC analysis determined cut-off points of the VSCOMB for diagnosis of CA atherosclerosis of various severity. VSCOMB scores  >4 indicated pronounced CA atherosclerosis with sensitivity of 86.1 % and specificity of 87.5 % whereas VSCOMB scores  ≤4 excluded it. Thus, VSCOMB score 0–1 indicated the absence of CA atherosclerosis; score 2–4 indicated the presence of subclinical CA atherosclerosis; and score >4 indicated severe CA atherosclerosis.Conclusion      A VSCOMB was developed that includes a set of ultrasound parameters for CA and FA and is useful for noninvasive diagnosis of CA atherosclerosis of various severity. Simple and convenient use of VSCOMB allows it to be used at the screening stage to detect subclinical CA atherosclerosis and to prevent its progression. 

scholarly journals Sex determination in gibbons of genus Nomascus using non-invasive method

2016 ◽  
Vol 85 (4) ◽  
pp. 363-366 ◽  
Author(s):  
Petra Bolechová ◽  
Kateřina Ječmínková ◽  
Michal Hradec ◽  
Tomáš Kott ◽  
Jana Doležalová
Keyword(s):  
Sex Determination ◽  
Direct Examination ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Non Invasive ◽  
Faecal Samples ◽  
Invasive Method ◽  
Polymerase Chain ◽  
Template Dna

Gibbons of the genus Nomascus have a strong sexual dimorphism and dichromatism. As they mature, both sexes develop sex-specific pelage colour. In combination with physical similarities in the genitalia with both sexes, there are problems with determining the sex of young individuals compared to other genus of gibbons. This is a pilot study applying a multiplex polymerase chain reactions based on a non-invasive method for sex determination of gibbons. The study was conducted on 22 faecal samples from gibbons of the genus Nomascus. The animals were monitored by staff so that the samples were identified correctly and each sample was collected immediately after the defecation. Results confirmed the sex in all adult and juvenile animals with known sex; and 2 females and 5 males in juveniles were determined with unknown sex. The results of direct examination completely corresponded with the PCR results. The PCR reaction with template DNA isolated from faecal material required BSA usage, however, we observed the occurrence of nonspecific fragments. This did not affect the reliability of our results and we confirmed the usability of this method for this genus.

scholarly journals A Non-invasive Method for the Determination of Liquid Injectables by Raman Spectroscopy

2015 ◽  
Vol 16 (4) ◽  
pp. 914-921 ◽  
Author(s):  
Yu Zhao ◽  
Nan Ji ◽  
Lihui Yin ◽  
Jun Wang
Keyword(s):  
Raman Spectroscopy ◽  
Non Invasive ◽  
Invasive Method

Non-invasive Prenatal Determination of Fetal Maturity

2016 ◽  
Vol 70 (3) ◽  
pp. 153-157
Author(s):  
Elena Dzikova ◽  
Goran Dimitrov ◽  
Olivera Stojceva-Taneva
Keyword(s):  
Gestational Age ◽  
Lamellar Body ◽  
Invasive Technique ◽  
Non Invasive ◽  
Gynecology And Obstetrics ◽  
Fetal Maturity ◽  
Invasive Method ◽  
The University ◽  
Body Count

Abstract Aims. The prenatal prediction of fetal maturity is very important, since neonatal respiratory distress syndrome (RDS) is one of the biggest causes of neonatal mortality. Our aim was to investigate a new non-invasive method for prediction of fetal maturity and to determine in which group according to gestational age of the fetus, the treatment works the best and in which cases it is necessary to be repeated. Methods. We examined 60 patients (30 with impending preterm delivery, divided in 3 groups: 28-30, 30-32, and 32-34 gestational weeks and 30 controls), at the University Clinic for Gynecology and Obstetrics, Medical Faculty, University “Ss. Cyril and Methodius”, Skopje, R. Macedonia. Fetal maturity was examined using ultrasound histogram from fetal lungs and liver, correlated with gestational age and postpartum RDS. Where possible, we performed amniocentesis for lamellar body count (LBC) to correlate our results with the current invasive method for prediction of fetal maturity. Results. Pre-therapy investigation showed a strong fetal immaturity in 28-32 weeks of gestation and less evident fetal immaturity in 32-34 weeks of gestation. Seventy-two hours post-treatment, fetal maturation was low in the first group, higher in the second and the highest in the third group. Amniocentesis for LBC showed correlation with the ultrasound results. Postpartum results were correlated with pre-delivery ultrasound and showed significance of p <0.05. Conclusion. The results obtained in our study were with high significance, and they were in correlation with other similar studies. However, more extensive investigations should be made to replace the current invasive technique.

Relationship between milk storage characteristics and the short-term response of dairy cows to thrice-daily milking

1994 ◽  
Vol 58 (2) ◽  
pp. 181-187 ◽  
Author(s):  
R. J. Dewhurst ◽  
C. H. Knight
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Short Term ◽  
Additional Time ◽  
Lactating Dairy Cows ◽  
Total Milk ◽  
Milk Storage ◽  
The Relationship ◽  
Storage Characteristics ◽  
Term Response

AbstractTwenty lactating dairy cows were used to investigate the relationship between the site of milk storage in the udder and the short-term response to thrice-daily milking. Cisternal and alveolar milk volumes were measured 8 h after an ordinary morning milking by catheter drainage and machine milking with oxytocin respectively. The response to thrice-daily milking was assessed using a half-udder technique and the relative milk yields quotient (RMYQ). Over the first 7 days, both halves were milked twice daily (8/16 h intervals) and milk yields over the final 4 days of this period were higher for left fore/right hind (LF/RH) (12·4 (s.e. 0·85) kg/day) than for RF/LH (10·5 (s.e. 0·63) kg/day) which was milked after LF/RH throughout the experiment. Over the following week, LF/RH quarters were milked an additional time (8/8/8 h intervals) and yields over the final 4 days were increased (15•7 (s.e. 0·95) kg/day) compared with control quarters (9·8 (s.e. 0·73) kg/day). In a final 4-day period, animals were milked twice daily and half udder yields were 13·1 (s.e. 0·89) kg/day and 10•6 (s.e. 0·77) kg/day respectively. Differences between yields from the two halves of the udders were highly significant in all 3 weeks of the experiment (P < 0·001). Cistern milk yield as a proportion of total milk yield at 8 h (cistern proportion) averaged 0·170 (s.e. = 0·0275; range 0·020 to 0·334) and tended to be greater for multiparous (0·215, s.e. 0·0279) than for primiparous animals (0·118, s.e. 0·0437; P = 0·076). During the periods of twice-daily milking, the proportion of milk yielded from LF/RH quarters was not significantly related to cistern proportion (P = 0·70 and 0·43 for weeks 1 and 3 respectively). However the response to thrice-daily milking, assessed as RMYQ, was significantly related to cistern proportion both when changing up to, and down from, thrice-daily milking (P < 0·01). Animals with low cistern proportions showed larger responses to thrice-daily milking. There was a significant relationship (P < 0·05) between the responses on changing up to, and down from, thrice-daily milking. Primiparous animals tended to exhibit smaller declines on returning to twice-daily milking than multiparous animals with equivalent responses to thrice-daily milking.

scholarly journals A method for the preservation and determination of welding fume nanoparticles in exhaled breath condensate

2016 ◽  
Vol 3 (2) ◽  
pp. 357-364 ◽  
Author(s):  
Sabrina Gschwind ◽  
Halshka Graczyk ◽  
Detlef Günther ◽  
Michael Riediker
Keyword(s):  
Exhaled Breath Condensate ◽  
Environmental Exposures ◽  
Exhaled Breath ◽  
Welding Fume ◽  
Non Invasive ◽  
Invasive Method ◽  
Inhaled Nanoparticles ◽  
Breath Condensate

Analysis of exhaled breath condensate (EBC) represents a non-invasive method for detecting inhaled nanoparticles (NPs) associated with various occupational and environmental exposures.

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