Camel (Camelus dromedarius) immunoglobulin G, α-lactalbumin, serum albumin and lactoferrin in colostrum and milk during the early post partum period

Colostrum and milk samples from twelve Tunisian camels were analysed for concentration of immunoglobulin G (IgG), α-lactalbumin (α-la), serum albumin (CSA) and lactoferrin throughout the first 14 milkings post partum (7 days of lactation) using single radial immunodiffusion assay. Concentrations (mg/ml, means±SD) at first milking were IgG, 100·7±60·4; α-la, 2·2±0·7; CSA, 8·5±3·6 and lactoferrin, 1·2±0·3. Large variations were recorded for IgG and CSA concentrations (11·8–211·1 mg/ml and 2·9–13·8 mg/ml respectively) Concentrations of IgG and CSA dropped abruptly in the subsequent milkings while α-la concentration increased until milking 5 and then decreased slowly. Lactoferrin dropped only from milking 7. Mean IgG concentrations were 3·6 and 2·5 mg/ml at milking 9 and 13 respectively. However, IgG concentration did not differ significantly, at the 1% level, from milkings 11 to 14. The contribution of CSA to the increase in whey proteins in early milks was greater than that described in the bovine and caprine species.

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Caprine immunoglobulin G, β-lactoglobulin, α-lactalbumin and serum albumin in colostrum and milk during the early post partum period

Journal of Dairy Research ◽

10.1017/s0022029902005575 ◽

2002 ◽

Vol 69 (3) ◽

pp. 391-399 ◽

Cited By ~ 27

Author(s):

DIDIER LEVIEUX ◽

FRANCOIS MORGAN ◽

NATHALIE GENEIX ◽

ISABELLE MASLE ◽

FREDERIC BOUVIER

Keyword(s):

Serum Albumin ◽

Immunoglobulin G ◽

Post Partum ◽

Whey Proteins ◽

Radial Immunodiffusion ◽

Milk Supply ◽

Single Radial Immunodiffusion ◽

Milk Samples ◽

Post Partum Period ◽

Β Lactoglobulin

Colostrum and milk samples from 20 goats were analysed for concentrations of immunoglobulin G (IgG), β-lactoglobulin (β-lg), α-lactalbumin (α-la) and serum albumin (CSA) throughout the first 14 milkings post partum (7 d of lactation) using single radial immunodiffusion assay. Concentrations (mg/ml, means±SD) at first milking were IgG 47·9±25·5, β-lg 30·7±10·4, α-la 2·77±0·82 and CSA 2·97±2·46 mg/ml. Large variations were recorded for IgG concentrations (19·9–94·5 mg/ml) and β-lg (9·3–49·8 mg/ml). Concentrations of IgG, β-lg and CSA dropped abruptly in the subsequent milkings and α-la concentration decreased slowly. Mean IgG concentration was <2 mg/ml after 7 milkings and <1 mg/ml after 11 milkings. However, IgG concentration does not differ significantly, at the 1% level, from milkings 7–14. The contribution of β-lg to the increase in whey proteins in early milks was greater than that of IgG from milkings 5 to 14. The results were tabulated to make it possible to calculate the excess of whey proteins that would be obtained if early milks were illegally added to milk supply.

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Bovine immunoglobulin G, β-lactoglobulin, α-lactalbumin and serum albumin in colostrum and milk during the early post partum period

Journal of Dairy Research ◽

10.1017/s0022029999003581 ◽

1999 ◽

Vol 66 (3) ◽

pp. 421-430 ◽

Cited By ~ 72

Author(s):

DIDIER LEVIEUX ◽

ALAIN OLLIER

Keyword(s):

Serum Albumin ◽

Immunoglobulin G ◽

Post Partum ◽

Whey Proteins ◽

Milk Supply ◽

Holstein Friesian ◽

The Mean ◽

Bovine Immunoglobulin ◽

Β Lactoglobulin ◽

Friesian Cows

Colostrum and milk samples from 60 Holstein–Friesian cows were analysed for concentrations and yields of immunoglobulin G (IgG), β-lactoglobulin (β-lg), α-lactalbumin (α-la) and serum albumin (BSA) throughout the first 16 milkings post partum (8 d of lactation) using a single radial immunodiffusion assay. Concentrations (mg/ml, means±SD) at first milking were IgG 59·8±28·5, β-lg 14·3±4·6, α-la 2·04±0·6, BSA 1·21±0·44. Large variations were recorded for IgG concentrations (15·3–176·2 mg/ml) and yields (0·2–925 g). Cows in their first lactation produced significantly lower concentrations and yields of colostral IgG than cows in later lactations. A colostral yield of IgG below the 100 g required to prevent calf hypo-γ-globulinaemia was found in 18·3% of the cows. The concentrations of IgG, β-lg and BSA dropped abruptly in subsequent milkings and α-la concentration decreased slowly. The mean IgG concentration was <2 mg/ml after eight milkings and <1 mg/ml after fifteen milkings. However, IgG concentration did not differ significantly, at the 1% level, during milkings 11–15. The results were tabulated to make it possible to calculate the excess of whey proteins that would be obtained if early milks were illegally added to the milk supply.

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Incidence of Colostrum in Raw Milk

Journal of Food Protection ◽

10.4315/0362-028x-56.7.625 ◽

1993 ◽

Vol 56 (7) ◽

pp. 625-626 ◽

Cited By ~ 5

Author(s):

JERZY ZAWISTOWSKI ◽

RUFINA MACKINNON

Keyword(s):

Immunoglobulin G ◽

Raw Milk ◽

Radial Immunodiffusion ◽

Milk Samples ◽

Bovine Immunoglobulin

A survey was conducted to determine the colostrum content in raw milk from dairies in Manitoba, Canada. Colostrum was indirectly measured by the determination of bovine immunoglobulin G (IgG) using a radial immunodiffusion assay. The results showed that 360 milk samples, which accounted for 89% of the total tested samples, were contaminated with colostrum. Of these, 320 samples had IgG levels in the range of 1.0 to 1.5 mg/ml, while 38 samples had an IgG content in the range of 1.5 to 2.0 mg/ml. Two milk samples contained IgG in excess of 2 mg/ml.

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Variation in colostral immunoglobulin G concentration in fat tailed sheep and evaluation of methods for estimation of colostral immunoglobulin content

Acta Veterinaria Brno ◽

10.2754/avb201382030271 ◽

2013 ◽

Vol 82 (3) ◽

pp. 271-275 ◽

Cited By ~ 5

Author(s):

Saeid Tabatabaei ◽

Gholamreza Nikbakht ◽

Mahmood Vatankhah ◽

Hamid Sharifi ◽

Naser Alidadi

Keyword(s):

Immunoglobulin G ◽

Zinc Sulphate ◽

Neonatal Period ◽

Radial Immunodiffusion ◽

Litter Type ◽

Maternal Factors ◽

Single Radial Immunodiffusion ◽

Biuret Method ◽

Turbidity Method ◽

Method R

In ruminants, colostrum is a vital source of immunoglobulins that provide passive immunity for their offspring during the neonatal period. It is suggested that colostral immunoglobulin G (IgG) concentration varies between and within breeds and could also be affected by maternal factors. The aim of this study was to investigate possible effects of litter type and ewe parturition number on colostral IgG concentration in two Iranian fat-tailed breeds of sheep (Shaul and Lori Bakhtyari) as well as usefulness of different methods for estimation of IgG concentrations in colostrum. The colostral IgG concentrations were measured in 38 Shaul and 59 Lori Bakhtyari ewes by single radial immunodiffusion, zinc sulphate turbidity and Biuret methods. Measurement of IgG by single radial immunodiffusion revealed that Lori Bakhtyari ewes had significantly (P < 0.05) lower colostral IgG levels (48.82 ± 2.10 mg/ml) than Shaul ewes (62.86 ± 2.48). With regard to the effect of litter type and parturition number, a significant (P < 0.05) difference in IgG concentration of colostrum was only observed between the first (65.17 ± 5.74 mg/ml) and third parturition (41.10 ± 4.60 mg/ml) of Lori Bakhtyari ewes. The colostral IgG concentration was not associated with ewe serum IgG concentration (P > 0.05). The mortality rate was higher in lambs born to ewes with lower IgG in their colostrum. Single radial immunodiffusion did not correlate either with zinc sulphate turbidity method (r = -0.253, P > 0.05) or with Biuret method (r = -0.005, P > 0.05). We can conclude that concentration of colostral IgG could be influenced by breed but not by litter type and parturition number.

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Analysis on the effect of the various factors on immunoglobulin G in goat colostrum

E3S Web of Conferences ◽

10.1051/e3sconf/202123302042 ◽

2021 ◽

Vol 233 ◽

pp. 02042

Author(s):

Xiaoqing Shao ◽

Ming Cheng ◽

Xiaoning Zhang ◽

Cunfang Wang ◽

Hua Jiang

Keyword(s):

Citric Acid ◽

Immunoglobulin G ◽

Radial Immunodiffusion ◽

Lactation Period ◽

Freezing Time ◽

Single Radial Immunodiffusion ◽

Maximum Value

The present study aimed to determinate the content of immunoglobulin G (IgG) in goat colostrum and the effected factors were discussed by single radial immunodiffusion. The results showed that the highest level of IgG was detected in the first day after partum, and decreased quickly over the length of the lactation period. No significant effect on IgG was seen with different ways of thawing and the different freezing time. IgG was almost completely lost at 85°C for 2 min. The maximum value of IgG was observed at pH 6.5, and it reduced significantly when the pressure was higher than 500MPa, and decreased with the increase of the concentrations of citric acid and Ca2+.

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