Caprine immunoglobulin G, β-lactoglobulin, α-lactalbumin and serum albumin in colostrum and milk during the early post partum period

2002 ◽  
Vol 69 (3) ◽  
pp. 391-399 ◽  
Author(s):  
DIDIER LEVIEUX ◽  
FRANCOIS MORGAN ◽  
NATHALIE GENEIX ◽  
ISABELLE MASLE ◽  
FREDERIC BOUVIER
Keyword(s):  
Serum Albumin ◽  
Immunoglobulin G ◽  
Post Partum ◽  
Whey Proteins ◽  
Radial Immunodiffusion ◽  
Milk Supply ◽  
Single Radial Immunodiffusion ◽  
Milk Samples ◽  
Post Partum Period ◽  
Β Lactoglobulin

Colostrum and milk samples from 20 goats were analysed for concentrations of immunoglobulin G (IgG), β-lactoglobulin (β-lg), α-lactalbumin (α-la) and serum albumin (CSA) throughout the first 14 milkings post partum (7 d of lactation) using single radial immunodiffusion assay. Concentrations (mg/ml, means±SD) at first milking were IgG 47·9±25·5, β-lg 30·7±10·4, α-la 2·77±0·82 and CSA 2·97±2·46 mg/ml. Large variations were recorded for IgG concentrations (19·9–94·5 mg/ml) and β-lg (9·3–49·8 mg/ml). Concentrations of IgG, β-lg and CSA dropped abruptly in the subsequent milkings and α-la concentration decreased slowly. Mean IgG concentration was <2 mg/ml after 7 milkings and <1 mg/ml after 11 milkings. However, IgG concentration does not differ significantly, at the 1% level, from milkings 7–14. The contribution of β-lg to the increase in whey proteins in early milks was greater than that of IgG from milkings 5 to 14. The results were tabulated to make it possible to calculate the excess of whey proteins that would be obtained if early milks were illegally added to milk supply.

Camel (Camelus dromedarius) immunoglobulin G, α-lactalbumin, serum albumin and lactoferrin in colostrum and milk during the early post partum period

2006 ◽  
Vol 73 (3) ◽  
pp. 288-293 ◽  
Author(s):  
Halima El-Hatmi ◽  
Annie Levieux ◽  
Didier Levieux
Keyword(s):  
Serum Albumin ◽  
Immunoglobulin G ◽  
Post Partum ◽  
Whey Proteins ◽  
Radial Immunodiffusion ◽  
Camelus Dromedarius ◽  
Single Radial Immunodiffusion ◽  
Milk Samples ◽  
Post Partum Period

Colostrum and milk samples from twelve Tunisian camels were analysed for concentration of immunoglobulin G (IgG), α-lactalbumin (α-la), serum albumin (CSA) and lactoferrin throughout the first 14 milkings post partum (7 days of lactation) using single radial immunodiffusion assay. Concentrations (mg/ml, means±SD) at first milking were IgG, 100·7±60·4; α-la, 2·2±0·7; CSA, 8·5±3·6 and lactoferrin, 1·2±0·3. Large variations were recorded for IgG and CSA concentrations (11·8–211·1 mg/ml and 2·9–13·8 mg/ml respectively) Concentrations of IgG and CSA dropped abruptly in the subsequent milkings while α-la concentration increased until milking 5 and then decreased slowly. Lactoferrin dropped only from milking 7. Mean IgG concentrations were 3·6 and 2·5 mg/ml at milking 9 and 13 respectively. However, IgG concentration did not differ significantly, at the 1% level, from milkings 11 to 14. The contribution of CSA to the increase in whey proteins in early milks was greater than that described in the bovine and caprine species.

Bovine immunoglobulin G, β-lactoglobulin, α-lactalbumin and serum albumin in colostrum and milk during the early post partum period

1999 ◽  
Vol 66 (3) ◽  
pp. 421-430 ◽  
Author(s):  
DIDIER LEVIEUX ◽  
ALAIN OLLIER
Keyword(s):  
Serum Albumin ◽  
Immunoglobulin G ◽  
Post Partum ◽  
Whey Proteins ◽  
Milk Supply ◽  
Holstein Friesian ◽  
The Mean ◽  
Bovine Immunoglobulin ◽  
Β Lactoglobulin ◽  
Friesian Cows

Colostrum and milk samples from 60 Holstein–Friesian cows were analysed for concentrations and yields of immunoglobulin G (IgG), β-lactoglobulin (β-lg), α-lactalbumin (α-la) and serum albumin (BSA) throughout the first 16 milkings post partum (8 d of lactation) using a single radial immunodiffusion assay. Concentrations (mg/ml, means±SD) at first milking were IgG 59·8±28·5, β-lg 14·3±4·6, α-la 2·04±0·6, BSA 1·21±0·44. Large variations were recorded for IgG concentrations (15·3–176·2 mg/ml) and yields (0·2–925 g). Cows in their first lactation produced significantly lower concentrations and yields of colostral IgG than cows in later lactations. A colostral yield of IgG below the 100 g required to prevent calf hypo-γ-globulinaemia was found in 18·3% of the cows. The concentrations of IgG, β-lg and BSA dropped abruptly in subsequent milkings and α-la concentration decreased slowly. The mean IgG concentration was <2 mg/ml after eight milkings and <1 mg/ml after fifteen milkings. However, IgG concentration did not differ significantly, at the 1% level, during milkings 11–15. The results were tabulated to make it possible to calculate the excess of whey proteins that would be obtained if early milks were illegally added to the milk supply.

Comparison of the ability to bind lipids of β-lactoglobulin and serum albumin of milk from ruminant and non-ruminant species

1993 ◽  
Vol 60 (1) ◽  
pp. 55-63 ◽  
Author(s):  
M. Dolores Pérez ◽  
Pilar Puyol ◽  
José Manuel Ena ◽  
Miguel Calvo
Keyword(s):  
Fatty Acids ◽  
Guinea Pig ◽  
Serum Albumin ◽  
Whey Protein ◽  
Whey Proteins ◽  
Gel Filtration ◽  
Ruminant Species ◽  
Β Lactoglobulin

SummaryThe interaction of sheep, horse, pig, human and guinea-pig whey proteins with fatty acids has been studied. Using gel filtration and autoradiography, it was found that sheep β-lactoglobulin and serum albumin from all species had the ability to bind fatty acids in vitro. Sheep β-lactoglobulin, isolated from milk, had ˜ 0·5 mol fatty acids bound per mol monomer protein, and albumin from sheep, horse and pig contained ˜ 4·5, 2·9 and 4·7 mol fatty acids/mol protein respectively. However, β-lactoglobulin from horse and pig milk had neither fatty acids physiologically bound nor the ability to bind them in vitro. Albumin was the only whey protein detected with bound fatty acids in these species as well as in human and guinea pig. This suggests that the ability of ruminant β-lactoglobulin to bind fatty acids was not shared by the same protein of non-ruminants.

Incidence of Colostrum in Raw Milk

1993 ◽  
Vol 56 (7) ◽  
pp. 625-626 ◽  
Author(s):  
JERZY ZAWISTOWSKI ◽  
RUFINA MACKINNON
Keyword(s):  
Immunoglobulin G ◽  
Raw Milk ◽  
Radial Immunodiffusion ◽  
Milk Samples ◽  
Bovine Immunoglobulin

A survey was conducted to determine the colostrum content in raw milk from dairies in Manitoba, Canada. Colostrum was indirectly measured by the determination of bovine immunoglobulin G (IgG) using a radial immunodiffusion assay. The results showed that 360 milk samples, which accounted for 89% of the total tested samples, were contaminated with colostrum. Of these, 320 samples had IgG levels in the range of 1.0 to 1.5 mg/ml, while 38 samples had an IgG content in the range of 1.5 to 2.0 mg/ml. Two milk samples contained IgG in excess of 2 mg/ml.

scholarly journals Variation in colostral immunoglobulin G concentration in fat tailed sheep and evaluation of methods for estimation of colostral immunoglobulin content

2013 ◽  
Vol 82 (3) ◽  
pp. 271-275 ◽  
Author(s):  
Saeid Tabatabaei ◽  
Gholamreza Nikbakht ◽  
Mahmood Vatankhah ◽  
Hamid Sharifi ◽  
Naser Alidadi
Keyword(s):  
Immunoglobulin G ◽  
Zinc Sulphate ◽  
Neonatal Period ◽  
Radial Immunodiffusion ◽  
Litter Type ◽  
Maternal Factors ◽  
Single Radial Immunodiffusion ◽  
Biuret Method ◽  
Turbidity Method ◽  
Method R

In ruminants, colostrum is a vital source of immunoglobulins that provide passive immunity for their offspring during the neonatal period. It is suggested that colostral immunoglobulin G (IgG) concentration varies between and within breeds and could also be affected by maternal factors. The aim of this study was to investigate possible effects of litter type and ewe parturition number on colostral IgG concentration in two Iranian fat-tailed breeds of sheep (Shaul and Lori Bakhtyari) as well as usefulness of different methods for estimation of IgG concentrations in colostrum. The colostral IgG concentrations were measured in 38 Shaul and 59 Lori Bakhtyari ewes by single radial immunodiffusion, zinc sulphate turbidity and Biuret methods. Measurement of IgG by single radial immunodiffusion revealed that Lori Bakhtyari ewes had significantly (P < 0.05) lower colostral IgG levels (48.82 ± 2.10 mg/ml) than Shaul ewes (62.86 ± 2.48). With regard to the effect of litter type and parturition number, a significant (P < 0.05) difference in IgG concentration of colostrum was only observed between the first (65.17 ± 5.74 mg/ml) and third parturition (41.10 ± 4.60 mg/ml) of Lori Bakhtyari ewes. The colostral IgG concentration was not associated with ewe serum IgG concentration (P > 0.05). The mortality rate was higher in lambs born to ewes with lower IgG in their colostrum. Single radial immunodiffusion did not correlate either with zinc sulphate turbidity method (r = -0.253, P > 0.05) or with Biuret method (r = -0.005, P > 0.05). We can conclude that concentration of colostral IgG could be influenced by breed but not by litter type and parturition number.

Correlations between the content of selected whey proteins in cow milk

2017 ◽  
Vol 13 (1) ◽  
pp. 33-45
Author(s):  
Aneta Brodziak ◽  
Jolanta Król ◽  
Anna Litwińczuk ◽  
Anna Wolanciuk
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Whey Proteins ◽  
Correlation Coefficients ◽  
Hplc Method ◽  
Cow Milk ◽  
Black And White ◽  
Scatter Plots ◽  
Β Lactoglobulin

The aim of the study was to determine the correlations between the concentrations of major whey proteins in cow milk. A total of 2,278 milk samples from Polish Holstein-Friesian (Black-and-White and Red-and-White varieties), Simmental and Jersey cows were analysed. In each sample the content of major whey proteins, i.e. α-lactalbumin, β-lactoglobulin, bovine serum albumin, lactoferrin and lysozyme were determined by the RP-HPLC method. Matrix scatter plots were prepared to determine the correlations between the concentrations of individual whey proteins. In the vast majority of cases a significant relationship was found between the content of individual whey proteins. Taking into account the production season and breed of cow, highly significant (p=0.001) negative correlation coefficients were obtained for the content of α-lactalbumin and bovine serum albumin, for α-lactalbumin and lysozyme, for β-lactoglobulin and bovine serum albumin, and for β-lactoglobulin and lysozyme. Positive correlations were observed for the concentrations of α-lactalbumin with β-lactoglobulin, lactoferrin and lysozyme, as well as for bovine serum albumin with lysozyme.

Mare milk nitrogen fractions during lactation and determination by reversed-phase HPLC of the major whey proteins

2005 ◽  
Vol 85 (1) ◽  
pp. 93-99 ◽  
Author(s):  
A. Summer ◽  
A. Tirelli ◽  
P. Formaggioni ◽  
M. Malacarne ◽  
P. Mariani
Keyword(s):  
Whey Protein ◽  
Post Partum ◽  
Whey Proteins ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Protein Distribution ◽  
Total N ◽  
Nitrogen Fractions ◽  
Lactation Stage ◽  
Β Lactoglobulin

The aim of this research was to describe the changes of the nitrogen fractions in milk of Haflinger nursing mares and in particular to determine the whey protein content and distribution, and their evolution throughout the first 6 mo of lactation. Samples were collected by hand-milking on days 4, 20, 40, 60, 80, 120, 150 and 180 post-partum. Nitrogen fractions were determined by Kjeldahl on 80 samples from 10 mares, while HPLC separation of whey proteins was conducted on 40 samples from 5 mares. The total N, casein N, and true whey protein N contents showed a statistically significant decrease from day 4 to day 40, and then remained unvaried. In general, the nitrogen distribution of mare’s milk significantly changed between day 4 and day 20 and then remained almost unchanged until day 180 (except for the day 150 value, which showed a statistically significant increase for CN × 100/TN). β-Lactoglobulin and serum albumin contents showed a marked reduction, of 27 and 45.5%, respectively, between day 4 and day 20, and then remained unchanged; α-lactalbumin and immunoglobulins contents had a reduction of 24.7 and 38.3%, respectively, between day 4 and day 20, and another decrease between day 20 and day 40, of 20 and 32.8%, respectively. All whey proteins, expressed as a percentage on the total sum of the four whey proteins considered, did not vary significantly during lactation. However, the sum on the two whey proteins of mammary gland origin (β-Lg + α-La) increased between day 4 and day 20 by 6.3%, and between day 20 and day 40 by 3.8%; the sum of the whey proteins of blood origin (SA + Ig) showed an opposite trend, with a decrease by 14.5% between day 4 and day 20 and by 11% between day 20 and day 40. Key words: Mare’s milk, lactation stage, whey protein distribution, reversed-phase HPLC

scholarly journals Analysis on the effect of the various factors on immunoglobulin G in goat colostrum

2021 ◽  
Vol 233 ◽  
pp. 02042
Author(s):  
Xiaoqing Shao ◽  
Ming Cheng ◽  
Xiaoning Zhang ◽  
Cunfang Wang ◽  
Hua Jiang
Keyword(s):  
Citric Acid ◽  
Immunoglobulin G ◽  
Radial Immunodiffusion ◽  
Lactation Period ◽  
Freezing Time ◽  
Single Radial Immunodiffusion ◽  
Maximum Value

The present study aimed to determinate the content of immunoglobulin G (IgG) in goat colostrum and the effected factors were discussed by single radial immunodiffusion. The results showed that the highest level of IgG was detected in the first day after partum, and decreased quickly over the length of the lactation period. No significant effect on IgG was seen with different ways of thawing and the different freezing time. IgG was almost completely lost at 85°C for 2 min. The maximum value of IgG was observed at pH 6.5, and it reduced significantly when the pressure was higher than 500MPa, and decreased with the increase of the concentrations of citric acid and Ca2+.

scholarly journals Effect of the feeding system and the production season on the protein fraction content in milk

2019 ◽  
Vol 69 (2) ◽  
pp. 98-107 ◽  
Author(s):  
Jolanta Król ◽  
Aneta Brodziak ◽  
Witold Chabuz ◽  
Zygmunt Litwińczuk ◽  
Joanna Barłowska
Keyword(s):  
Protein Fraction ◽  
Somatic Cell Count ◽  
Crude Protein ◽  
Whey Proteins ◽  
Correlation Coefficients ◽  
Feeding System ◽  
Milk Samples ◽  
Summer Pasture ◽  
Β Lactoglobulin ◽  
Group 1

The objective of the present research was to analyse the protein fraction content in milk, with particular regard to whey proteins, in dependence on the cows’ feeding system (group 1 - extensive, 2 - semi-intensive, 3 - intensive) and production season (spring-summer and autumn-winter). Chemical analysis of the fodder was the base for calculation of energy and protein coverage of nutritional dose. A total of 1,133 milk samples were evaluated (550 in winter and 583 in summer). The milk samples were examined for the somatic cell count (SCC), the basic chemical composition, casein and whey proteins: α-lactalbumin, β-lactoglobulin, bovine serum albumin (BSA), lactoferrin and lysozyme. Higher content of crude protein, including casein, was noticed in milk obtained from cows coming from semi-intensive and intensive farms. However, milk taken from cows fed according to the group 1, which was based on fodder from permanent grasslands, had the highest concentration of major whey proteins - both in the summer (pasture and hay) and winter (hay and haylage) seasons. With the increase of silage and industrial fodder in the feed ration, the content of whey proteins - mainly β-lactoglobulin and lactoferrin - decreased, which was confirmed by the obtained negative correlation coefficients.

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