Haplotypic variation in the UCP1 gene is associated with milk traits in dairy cows

2016 ◽  
Vol 84 (1) ◽  
pp. 68-75 ◽  
Author(s):  
Huitong Zhou ◽  
Long Cheng ◽  
Seung Ok Byun ◽  
Wumaierjiang Aizimu ◽  
Miriam C Hodge ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Uncoupling Protein ◽  
Lower Percentage ◽  
Fat Percentage ◽  
Phenotypic Effect ◽  
Milk Traits ◽  
Single Stranded Conformational Polymorphism ◽  
Holstein Friesian

Uncoupling protein-1 (UCP1) plays a role in the regulation of body temperature, metabolic rate and energy expenditure in animals. While variation in UCP1 and its phenotypic effect has been investigated in humans and sheep, little is known about this gene in cattle. In this study, four regions of bovine UCP1 were investigated in 612 Holstein-Friesian × Jersey (HF × J) dairy cows using polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analyses. In the four regions of the gene analysed, a total of 13 SNPs were detected. Three sequences (a, b and c) were found in Region-2 and three sequences (A, B and C) were found in Region-4, and these were assembled into three (a-B, b-B and c-A) common and three (b-C, c-B and c-C) rare haplotypes. Of the three common haplotypes, b-B and c-A were associated (P < 0·007 and P < 0·043, respectively) with increased milk yield and tended to be associated (P < 0·085 and P < 0·070, respectively) with decreased fat percentage. Cows with genotype b-B/a-B produced more milk (P < 0·004), but with a lower percentage of fat (P < 0·035) and protein (P < 0·038) than cows with genotype a-B/a-B. Cows of genotype a-B/c-A had milk of low fat percentage (P < 0·017), but tended to produce more milk (P < 0·059) than cows of genotype a-B/a-B. This suggests that UCP1 affects milk yield, milk fat percentage and milk protein percentage.

Variation in the Toll-like Receptor 4 (TLR4) gene affects milk traits in dairy cows

2017 ◽  
Vol 84 (4) ◽  
pp. 426-429 ◽  
Author(s):  
Huitong Zhou ◽  
Long Cheng ◽  
Hua Gong ◽  
Seung O. Byun ◽  
Grant R. Edwards ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Production Traits ◽  
Toll Like Receptor 4 ◽  
Milk Traits ◽  
Single Nucleotide ◽  
Gene Variation ◽  
Single Stranded Conformational Polymorphism ◽  
Holstein Friesian ◽  
Tlr4 Gene

The objective of this Research Communication was to use polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) analysis to investigate a region of the bovine TLR4 gene (TLR4) in pasture-fed New Zealand (NZ) Holstein-Friesian × Jersey (HF × J) cross dairy cows and to determine whether gene variation was associated with milk production traits. Genetic variation was observed, with two variants (A and B) containing a single nucleotide polymorphism (SNP) (c.2021C/T) that was non-synonymous and putatively results in a p.Thr674Ile substitution in the transmembrane/cytoplasmic domain of TLR4. Variant A was associated with higher milk yields, but lower milk fat percentages, whereas B was associated with lower milk yields, but higher fat and protein percentages. Cows of genotype AA produced more milk than AB or BB cows, but the milk produced by AA cows contained less fat than AB or BB cows.

The effects on milk yield and composition of incorporating lactose into the diet of dairy cows given protected fat

1996 ◽  
Vol 62 (1) ◽  
pp. 1-3 ◽  
Author(s):  
P. C. Garnsworthy
Keyword(s):  
Sugar Beet ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Protein Concentration ◽  
Milk Protein ◽  
Basal Diet ◽  
Fat Supplementation ◽  
Holstein Friesian ◽  
Group A

AbstractTwenty-eight Holstein/Friesian dairy cows were divided into four groups of seven. From weeks 4 to 15 of lactation they were given a basal diet consisting of 8 kg hay, 2 kg sugar-beet feed and 2 kg grass nuts, together with a concentrate allowance of 8 kg/day. Concentrates for group A were based on cereals and soya (control). Concentrate B contained 60 g protected fat supplement per kg; concentrate C contained 100 g lactose per kg; concentrate D contained 60 g fat supplement and 100 g lactose per kg. Milk yields were 24·6, 27·7, 25·6 and 26·5 kg/day and milk protein concentrations were 32·3, 30·7, 32·7 and 31·9 g/kg for groups A, B, C and D respectively. The effect of fat supplementation on milk yield and protein concentration was significant (P < 0·05) but the effect of lactose was not significant. Milk fat concentration was not significantly affected by treatment. It is concluded that lactose can partially alleviate the depression in milk protein concentration often observed when cows are given protected fat.

scholarly journals DAY-TO-DAY VARIATIONS IN FAT PERCENT OF COW'S MILK

1990 ◽  
Vol 70 (2) ◽  
pp. 731-734 ◽  
Author(s):  
A. S. ATWAL ◽  
J. D. ERFLE
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Milk Yield ◽  
Milk Fat ◽  
Rumen Fluid ◽  
Supplemental Feeding ◽  
Fat Percentage ◽  
Milk Fat Depression ◽  
Daily Milk Yield ◽  
Daily Milk

Large day-to-day variations in milk fat, particularly for the morning milkings, were observed in 36 Holstein cows. Changes in percent fat were gradual and produced wavelike patterns in a number of instances. Supplemental feeding of long hay had no effect on acetate/propionate ratio in rumen fluid, daily milk yield or weighted milk fat percentage. Key words: Dairy cows, milk, fat depression, hay

scholarly journals The effect of the farm size on milk traits of cows

2011 ◽  
Vol 27 (3) ◽  
pp. 951-958 ◽  
Author(s):  
D. Kucevic ◽  
S. Trivunovic ◽  
M. Radinovic ◽  
M. Plavsic ◽  
Z. Skalicki ◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Dairy Farm ◽  
Farm Size ◽  
Small Farms ◽  
Milk Traits ◽  
Holstein Friesian ◽  
Milk Performance ◽  
Fat Yield ◽  
Large Farms

Objective of this study was to analyze the effect of the dairy farm on milk traits of cows in Vojvodina. The research was carried out on small farms with 10 to 20 cows, medium farms with 20 to 50 cows, and large farms with over 50 dairy cows. The study included registered animals of Simmental (SM) and Holstein-Friesian breed (HF; including Red Holstein) in the first lactation for traits of milk yield and yield and content of milk fat. Total of 1323 first lactations were analyzed. The average milk yield (both breeds) in the first lactation of 305 days was 6295 kg of milk with 234,3 kg of milk fat and average milk fat content of 3,74%. Milk performance of cows varied significantly (CV=22,9% and SD=1447,8), as well as milk fat yield (CV=21,6% and SD=50,8). Large farms produced in average 6534 kg of milk, medium farms 6347kg and small farms 4717kg. Size of the farm exhibited significantly high effect on all observed traits, and the tendency was that farms with higher number of animals realize also higher average of production. Farm management and various breeding-zootechnical conditions present on farms had significant effect on milk performance of cows.

The relationship between milk progesterone and milk traits during estrus in dairy Cows

2018 ◽  
Author(s):  
A Gavelis ◽  
V. Þilaitis ◽  
A Juozaitis ◽  
V. Juozaitienë ◽  
G. Urbonavièius ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Protein Content ◽  
Milk Yield ◽  
Milk Fat ◽  
Milk Protein ◽  
Milk Traits ◽  
Subclinical Ketosis ◽  
Yield Level ◽  
Milk Protein Content ◽  
The Relationship

The aim of this study was to evaluate relationship between milk progesterone concentration (P4) and milk traits at the start of estrus time and 12h after start of the estrus in dairy cows. The 96 milk samples of 48 Lithuanian dairy cows without reproduction disorders and 90–100 days after calving were evaluated. Cows were classified into two groups based on milk yield per day: less than 30 kg (n=20) and e”30 kg (n=28). Data were categorized by milk fat and protein content at the start estrus and 12h after start of estrus to evaluate relationship between P4 and milk traits examined. P4 at estrus time in dairy cows was significantly positively correlated with milk yield (P less than 0.001), whereas it was negatively correlated with milk protein (P less than 0.05-P less than 0.01) and fat at 12h after start of estrus. Dairy cows with F/P from 1.0 to 1.5 had the lowest P4 in milk. Results of the pregnancy in dairy cows were related with lower P4 and milk yield level (P less than 0.001), higher milk fat (P less than 0.05) and milk protein content (P less than 0.001). These cows had 1.90 times lower prevalence of the signs of subclinical ketosis (P less than 0.05) at estrus time when compared with non-pregnant cows. As a result, it was clearly demonstrated that P4 in dairy cows can help to evaluate and improve the reproductive properties of cows.

scholarly journals Evaluation of Biomarkers of Heat Stress by Using Automatic Health Monitoring System in Dairy Cows

2020 ◽  
Author(s):  
Ramūnas Antanaitis ◽  
Vida Juozaitienė ◽  
Mindaugas Televičius ◽  
Dovilė Malašauskienė
Keyword(s):  
Body Weight ◽  
Heat Stress ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Management Program ◽  
Milk Traits ◽  
Protein Ratio ◽  
Time Body Weight ◽  
Automatic Milking

The objective of this study was to evaluate biomarkers of heat stress (HS) from automatic milking system (AMS), the relationships between measurements of temperature-humidity index (THI) and reticulorumen ph and temperature and some automatic milking systems parameters in dairy cows (rumination time (RT), milk traits, body weight (BW) and consumption of concentrate (CC)) during summer period. The experiment was carried out on a dairy farm at 54.9587408, 23.784146. Lithuanian Black and White dairy cows (n=365) were selected. The cows were milked with Lely Astronaut&reg; A3 milking robots with free traffic. The rations were calculated to meet physiological requirements of the animals. Daily milk yield, rumination time, body weight, milk fat and protein ratio were collected from the Lely T4C management program for analysis. The pH and temperature of the contents of cow reticulorumen were measured using specific smaX-tec boluses manufactured for animal care. The daily humidity and air temperature in the farm zone were obtained from the adjacent weather station (2 km away). According this study during HS, the higher THI had positive correlation with ML, which show tendencies to increase risk of mastitis, and decrease CC, RT, BW, MY, reticulorumen ph and F/P. Some biomarkers of HS can be milk yield, milk lactose, somatic cell count, concentrate conception, rumination time, body weight, reticulorumen ph and milk fat &ndash; protein ratio. We can recommend to monitoring these parameters in the herd management program to identify possibility of heat stress.

scholarly journals Feed sorting and intake affected by the physical form and composition of the total mixed ration in dairy cows

2015 ◽  
Vol 16 (3) ◽  
pp. 736-745
Author(s):  
Félix Hilario Diaz González ◽  
Diego Martínez Del Olmo ◽  
Rodrigo Muiño ◽  
José Luis Benedito ◽  
Joaquín Hernández ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Milk Protein ◽  
Dairy Farm ◽  
Milk Composition ◽  
Fat Percentage ◽  
Total Mixed Ration ◽  
Physical Form

AbstractThis study observes the effects of manipulating the diet in its physical form and in cereal composition of the concentrate on sorting behaviour, feed intake, composition of milk, and degree of discomfort in dairy cows. In a commercial dairy farm with 360 mid-lactation Holstein cows (averaged 105 days), three treatments of feeding handling were applied sequentially during 120 days, each separated by periods of 30 days of resting: (1) Time of chopping of the total mixed ration (TMR): 15min (N= 180) and 23min (N= 180), (2) Physical form of the concentrate: meal (N= 180) and pellet (N= 180), and (3) Cereal composition in the concentrate: mix of corn, barley and wheat in traditional proportions (3.6:1:1) (N= 120), barley only (N= 120) and corn only (N= 120). Animals feeding TMR chopped for 23min selected less and consumed more ration, and had more milk protein and less discomfort than cows feeding TMR chopped for 15min. The physical form of the concentrate, meal or pellet, did not affect feed sorting, but the meal form resulted in greater feed intake and milk fat percentage. The type of cereal in the concentrate did not affect feed sorting but affected feed intake, milk yield and milk composition. In conclusion, it is recommended using TMR chopped during 23min instead of 15min and using the traditional mix of cereals in the concentrate (corn, barn and wheat) instead of using only one cereal.

scholarly journals Impact of heat stress on milk yield and composition in early lactation of Holstein Friesian crossbred cattle

2018 ◽  
Vol 46 (3) ◽  
pp. 192-197 ◽  
Author(s):  
T Chanda ◽  
GK Debnath ◽  
KI Khan ◽  
MM Rahman ◽  
GC Chanda
Keyword(s):  
Heat Stress ◽  
Dairy Cows ◽  
Milk Yield ◽  
Rectal Temperature ◽  
Milk Fat ◽  
Lactation Period ◽  
Early Lactation ◽  
Cool Period ◽  
Holstein Friesian ◽  
Hot Season

The aim of the study was to know the effect of heat stress on milk yield and its composition of Holstein Friesian crossbred dairy cows rearing under intensive management system.Heat stress in dairy cows is caused by a combination of environmental factors i.e. temperature, relative humidity, solar radiation and air movement, etc. Grading-up of local cattle by temperate breeds for greater performance results to increase the sensitivity to heat stress. The trial was conducted during hot (May-July) and cool period (December-February) on a total of 12 crossbred dairy cows in early lactation period (first 60 days of lactation). The selected cows were divided into two groups namely as G1 (Holstein-Friesian 50% × Local 50%) and G2 (Holstein-Friesian 75% × Local 25%) and each group containing three cows of 3rd lactation in both periods of trail. Cows of each group were offered same quality and quantity of feed and reared in same management condition in both the trail periods. The mean average temperature humidity index (THI) of the stanchion barn were 70.83± 0.535 and 83.87± 0.375 in cool and hot period, respectively. The differences of THI of cool and hot season was significant (p<0.05). The average rectal temperature (0F) of G1 group was 101.51± 0.027and 102.15±0.049 in cool and hot period, respectively. On the other hand, the average rectal temperature (0F) of G2 group was 101.68± 0.035 and 102.5±0.052 in cool and hot period, respectively. The differences of rectal temperature of both groups between cool and hot season were significant (p<0.05). The average milk yield (Liter/day) of both groups was significantly (p<0.05) higher in cool period (14.92±0.019 and 19.54± 0.116 for G1 and G2 group, respectively) than in hot period (12.84±0.152 and 15±0.137 for G1 and G2 group, respectively). The milk yield of G2 group hampered more compare to G1 group due to higher THI during hot season. The milk fat, protein and lactose of both groups were significantly higher (p<0.05) during lower THI period compare to higher THI period, while the higher values of minerals detected in higher THI period but the differences were not statistically significant (p<0.05). From the result it is evident that the milk yield and composition are greatly affected by the heat stress during early lactation period.Bang. J. Anim. Sci. 2017. 46 (3): 192-197

scholarly journals Effect of Silage from Maize and Strip-Cropped Sorghum and Maize on Dairy Cow's Yield and Milk Composition

2012 ◽  
Vol 12 (3) ◽  
pp. 367-379 ◽  
Author(s):  
Bogdan Śliwiński ◽  
Franciszek Brzóska ◽  
Karol Węglarzy ◽  
Małgorzata Bereza
Keyword(s):  
Chemical Composition ◽  
Dairy Cows ◽  
Milk Yield ◽  
Production Efficiency ◽  
Milk Composition ◽  
Nutritional Requirement ◽  
Pure Stand ◽  
Maize Silage ◽  
Fat Percentage ◽  
Milk Traits

Effect of Silage from Maize and Strip-Cropped Sorghum and Maize on Dairy Cow's Yield and Milk CompositionIn an experiment conducted on 34 mid-lactation dairy cows of the Red-and-White and Black-and-White breed, in a random square design, the effect of maize or sorghum-maize silage present in partly mixed ration (PMR) on milk yield, milk composition and blood serum parameters was investigated. The PMR diet contained maize silage, which compared with PMR diet contained sorghum-maize silage made from strip-cropped plants. Both fodder plants were harvested with a 4-row precision chopper, which cut 2 rows of maize and 2 rows of sorghum, giving mixed maize/sorghum forage. The feeding experiment lasted 84 days and consisted of four sub-periods, each 21 days in length to record milk yield, feed and milk chemical composition, and blood parameters. In addition to PMR diets containing part of ration compound feedingstuffs, the cows received part of compound feedingstuffs given at feed stations to meet their nutritional requirement. The compound feedingstuffs in station were controlled by an electronic system related to actual cow's milk yield. Chemical composition of both silages and milk production efficiency were compared. The average dry matter intake in both groups was 18.80 vs 20.4±1.95 kg/day, but compound feedingstuff intake from station was 3.61 vs 4.56 (P>0.01). Milk yield was 21.8 and 20.5±0.51 kg/day, respectively (P>0.01). The amount of standardized fat and protein content of milk (FPCM) was 21.0 and 20.2 kg/g ±0.48 kg/day (P≥0.01). No significant differences were found in the fat, protein, casein, lactose, urea, total solids and solids not fat percentage of milk or in milk traits (acidity, renneting time, density) among groups (P≥0.01). Feeding cows PMR ration with maize silage significantly elevated total cholesterol (P<0.05), but decreased urea levels in blood plasma (P<0.01). It is concluded that strip cropping of sorghum and maize could be an alternative to maize grown as a pure stand in maize high-risk areas for dairy cows in mid-lactation.

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