Echinococcus granulosus strain differentiation in Iran based on sequence heterogeneity in the mitochondrial 12S rRNA gene

AbstractParasite strain characterization is essential for the establishment of a prevention and control strategy in any endemic area. The aim of this study was to characterize different Echinococcus granulosus isolates from Iran by using DNA sequences of the mitochondrial 12S rRNA gene. Thirty livers and lungs of cattle, sheep and goats naturally infected with E. granulosus were collected from abattoirs in northern and western Iran between June and October 2007. These samples yielded 18 fertile cysts which we used for the genetic work. We designed and tested two new primer pairs which specifically amplify portions of the mitochondrial 12S rRNA gene of the two strains (G1 and G6) of E. granulosus known to occur in Iran. One primer pair amplified a fragment of 259 base pairs (bp) from only the G1 strain. The second pair amplified a fragment of 676 bp from the G6 strain. The G1 genotype was identified in all fertile cyst samples, in agreement with previous studies in Iran. Ten of our samples and a single reference sample of the G6 strain were sequenced and compared with the G1 and G6 sequences deposited in GenBank.

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Identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene

Meat Science ◽

10.1016/j.meatsci.2006.11.004 ◽

2007 ◽

Vol 76 (2) ◽

pp. 234-240 ◽

Cited By ~ 42

Author(s):

V. Fajardo ◽

I. González ◽

I. López-Calleja ◽

I. Martín ◽

M. Rojas ◽

...

Keyword(s):

Cervus Elaphus ◽

Roe Deer ◽

Capreolus Capreolus ◽

Fallow Deer ◽

12S Rrna ◽

Rrna Gene ◽

12S Rrna Gene ◽

Mitochondrial 12S Rrna ◽

Polymerase Chain ◽

Specific Sequences

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Nucleotide sequence variation in the mitochondrial 12S rRNA gene and the phylogeny of African mole-rats (Rodentia: Bathyergidae).

Molecular Biology and Evolution ◽

10.1093/oxfordjournals.molbev.a040706 ◽

1992 ◽

Cited By ~ 1

Keyword(s):

Nucleotide Sequence ◽

Sequence Variation ◽

12S Rrna ◽

Rrna Gene ◽

12S Rrna Gene ◽

Nucleotide Sequence Variation ◽

Mitochondrial 12S Rrna

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Mitochondrial 12S rRNA gene mutations affect RNA secondary structure and lead to variable penetrance in hearing impairment

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2006.01.049 ◽

2006 ◽

Vol 341 (4) ◽

pp. 950-957 ◽

Cited By ~ 49

Author(s):

Ester Ballana ◽

Estela Morales ◽

Raquel Rabionet ◽

Bàrbara Montserrat ◽

Marina Ventayol ◽

...

Keyword(s):

Secondary Structure ◽

Hearing Impairment ◽

Rna Secondary Structure ◽

Gene Mutations ◽

12S Rrna ◽

Rrna Gene ◽

12S Rrna Gene ◽

Mitochondrial 12S Rrna ◽

Variable Penetrance

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Maternally transmitted aminoglycoside-induced and non-syndromic hearing loss caused by the 1494C > T mutation in the mitochondrial 12S rRNA gene in two Chinese families

International Journal of Audiology ◽

10.3109/14992027.2012.743046 ◽

2012 ◽

Vol 52 (2) ◽

pp. 98-103 ◽

Cited By ~ 3

Author(s):

Qinjun Wei ◽

Dan Xu ◽

Zhibin Chen ◽

Haifeng Li ◽

Yajie Lu ◽

...

Keyword(s):

Hearing Loss ◽

12S Rrna ◽

Rrna Gene ◽

Chinese Families ◽

12S Rrna Gene ◽

Mitochondrial 12S Rrna ◽

Syndromic Hearing Loss

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Phylogenetic Relationships among the Species of the Genus Testudo (Testudines: Testudinidae) Inferred from Mitochondrial 12S rRNA Gene Sequences

Molecular Phylogenetics and Evolution ◽

10.1006/mpev.2001.1052 ◽

2002 ◽

Vol 22 (2) ◽

pp. 174-183 ◽

Cited By ~ 48

Author(s):

Antoinette C. van der Kuyl ◽

Donato L. Ph. Ballasina ◽

John T. Dekker ◽

Jolanda Maas ◽

Ronald E. Willemsen ◽

...

Keyword(s):

Phylogenetic Relationships ◽

12S Rrna ◽

Rrna Gene ◽

Gene Sequences ◽

12S Rrna Gene ◽

Mitochondrial 12S Rrna

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Reassessment of the mitochondrial 12S-rRNA gene for DNA barcoding of museum specimens of shelled marine gastropods from Japan

E3S Web of Conferences ◽

10.1051/e3sconf/202132201028 ◽

2021 ◽

Vol 322 ◽

pp. 01028

Author(s):

Nao Fukunaga ◽

Moe Shimizu ◽

Shinnosuke Teruya ◽

Nazifa Naziha Razali ◽

Satoko Nakashima ◽

...

Keyword(s):

Dna Barcoding ◽

12S Rrna ◽

Rrna Gene ◽

Museum Specimens ◽

Biodiversity Monitoring ◽

Marine Gastropods ◽

12S Rrna Gene ◽

Reference Databases ◽

Mitochondrial 12S Rrna ◽

Comprehensive Reference

DNA barcoding is an effective and powerful tool for taxonomic identification and thus very useful for biodiversity monitoring. This study investigated the usefulness of the mitochondrial 12S-rRNA gene for the DNA barcoding of shelled marine gastropods. To do so, we determined partial 12S-rRNA sequences of 75 vouchered museum specimens from 69 species of shelled gastropods from Japan. The specimens have been identified morphologically, and natural history data catalog. Sequence analyses through BLAST searches, maximum likelihood phylogenetic analysis, and species delimitation analysis suggested that the 12S-rRNA gene is helpful for barcoding shelled marine gastropods. They thus could be helpful to complement barcoding studies using other markers such as COI. The analyses successfully confirmed all samples’ identity at higher taxonomy (subfamily and above), but much less so at the species level. Our result thus also underlines the lingering problem of DNA barcoding: The lack of comprehensive reference databases of sequences. However, since we provided sequences of properly curated, vouchered museum specimens in this study, our result reported here has thus also helped to give taxonomically reliable reference sequences for biodiversity monitoring and identifications of shelled gastropods which include many important fisheries species.

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Whole mitochondrial genome screening in two families with hearing loss: detection of a novel mutation in the 12S rRNA gene

Bioscience Reports ◽

10.1042/bsr20090120 ◽

2010 ◽

Vol 30 (6) ◽

pp. 405-411 ◽

Cited By ~ 4

Author(s):

Emna Mkaouar-Rebai ◽

Nourhene Fendri-Kriaa ◽

Nacim Louhichi ◽

Abdelaziz Tlili ◽

Chahnez Triki ◽

...

Keyword(s):

Hearing Loss ◽

Mitochondrial Genome ◽

Novel Mutation ◽

Hot Spot ◽

12S Rrna ◽

Rrna Gene ◽

12S Rrna Gene ◽

Genome Screening ◽

Mitochondrial 12S Rrna ◽

Syndromic Hearing Loss

Sensorineural hearing loss has been described in association with different mitochondrial multisystemic syndromes, often characterized by an important neuromuscular involvement. Until now, mutations in mitochondrial DNA, especially in the 12S rRNA, the tRNASer(UCN) and the tRNALeu(UUR) genes, were implicated in syndromic or non-syndromic hearing loss either as a primary cause or as predisposing factors. In the present study, we performed a whole mitochondrial genome screening in two unrelated Tunisian families with inherited hearing loss. Results showed the presence of a novel mutation in the mitochondrial 12S rRNA gene in the two probands of these two families who belong to two different haplogroups: L3 and H6a1. The m.735A>G mutation affects a conserved nucleotide of the mitochondrial 12S rRNA gene in primates and other species and had a conservation index of 78.5% (11/14). We also detected known polymorphisms and sic novel mitochondrial variants. The present study confirmed that the mitochondrial 12S rRNA gene is a hot spot for mutations associated with hearing impairment.

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