Morphometric and molecular descriptions of three new species of Hysterothylacium (Nematoda: Raphidascarididae) from Australian marine fish

2016 ◽  
Vol 91 (5) ◽  
pp. 613-624 ◽  
Author(s):  
S. Shamsi
Keyword(s):  
New Species ◽  
Marine Fish ◽  
Sequence Data ◽  
Internal Transcribed Spacers ◽  
Its Sequences ◽  
Larval Type ◽  
Third Stage ◽  
Three New Species ◽  
Paratenic Hosts ◽  
Seriola Lalandi

AbstractThree new species of Hysterothylacium Ward & Magath, 1917, including H. australe, H. kajikiae and H. brucei, from Australian marine fish are described and illustrated by light microscopy followed by genetic characterization of their first and second internal transcribed spacers (ITS-1 and ITS-2, respectively). This is the first study reporting ITS sequence data for adult Hysterothylacium spp. in Australia, which provides an insight into the identification of some of the Hysterothylacium larval types. Alignment of ITS sequences of these species with Hysterothylacium larval types previously reported in Australia showed that fourth-stage Hysterothylacium larval type XI from Seriola lalandi and third-stage Hysterothylacium larval type X from Sphyraene novae-hollandiae are identical with ITS sequences of H. australe, suggesting that these fish are natural intermediate/paratenic hosts of H. australe.

scholarly journals Occurrence ofTerranovalarval types (Nematoda: Anisakidae) in Australian marine fish with comments on their specific identities

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1722 ◽  
Author(s):  
Shokoofeh Shamsi ◽  
Jaydipbhai Suthar
Keyword(s):  
Marine Fish ◽  
Sequence Data ◽  
Infected Fish ◽  
Human Infection ◽  
Internal Transcribed Spacers ◽  
Accurate Identification ◽  
Essential Information ◽  
Larval Type ◽  
Morphological Criteria ◽  
Specific Identity

Pseudoterranovosis is a well-known human disease caused by anisakid larvae belonging to the genusPseudoterranova. Human infection occurs after consuming infected fish. Hence the presence ofPseudoterranovalarvae in the flesh of the fish can cause serious losses and problems for the seafood, fishing and fisheries industries. The accurate identification ofPseudoterranovalarvae in fish is important, but challenging because the larval stages of a number of different genera, includingPseudoterranova,TerranovaandPulchrascaris, look similar and cannot be differentiated from each other using morphological criteria, hence they are all referred to asTerranovalarval type. Given thatTerranovalarval types in seafood are not necessarilyPseudoterranovaand may not be dangerous, the aim of the present study was to investigate the occurrence ofTerranovalarval types in Australian marine fish and to determine their specific identity. A total of 137 fish belonging to 45 species were examined.Terranovalarval types were found in 13 species, some of which were popular edible fish in Australia. The sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2 respectively) of theTerranovalarvae in the present study showed a high degree of similarity suggesting that they all belong to the same species. Due to the lack of a comparable sequence data of a well identified adult in the GenBank database the specific identity ofTerranovalarval type in the present study remains unknown. The sequence of the ITS regions of theTerranovalarval type in the present study and those ofPseudoterranovaspp. available in GenBank are significantly different, suggesting that larvae found in the present study do not belong to the genusPseudoterranova, which is zoonotic. This study does not rule out the presence ofPseudoterranovalarvae in Australian fish asPseudoterranova decipiens Ehas been reported in adult form from seals in Antarctica and it is known that they have seasonal presence in Australian southern coasts. The genetic distinction ofTerranovalarval type in the present study fromPseudoterranovaspp. along with the presence of more species of elasmobranchs in Australian waters (definitive hosts ofTerranovaspp. andPulchrascarisspp.) than seals (definitive hosts ofPseudoterranovaspp.) suggest thatTerranovalarval type in the present study belong to either genusTerranovaorPulchrascaris, which are not known to cause disease in humans. The present study provides essential information that could be helpful to identify AustralianTerranovalarval types in future studies. Examination and characterisation of further specimens, especially adults ofTerranovaandPulchrascaris, is necessary to fully elucidate the identity of these larvae.

scholarly journals The Evolution of Life Modes in Stictidaceae, with Three Novel Taxa

2021 ◽  
Vol 7 (2) ◽  
pp. 105
Author(s):  
Vinodhini Thiyagaraja ◽  
Robert Lücking ◽  
Damien Ertz ◽  
Samantha C. Karunarathna ◽  
Dhanushka N. Wanasinghe ◽  
...  
Keyword(s):  
New Species ◽  
Large Scale ◽  
Sequence Data ◽  
Large Subunit ◽  
Monte Carlo Sampling ◽  
Small Subunit ◽  
Sensu Stricto ◽  
Internal Transcribed Spacers ◽  
Character State ◽  
Phenotypic Data

Ostropales sensu lato is a large group comprising both lichenized and non-lichenized fungi, with several lineages expressing optional lichenization where individuals of the same fungal species exhibit either saprotrophic or lichenized lifestyles depending on the substrate (bark or wood). Greatly variable phenotypic characteristics and large-scale phylogenies have led to frequent changes in the taxonomic circumscription of this order. Ostropales sensu lato is currently split into Graphidales, Gyalectales, Odontotrematales, Ostropales sensu stricto, and Thelenellales. Ostropales sensu stricto is now confined to the family Stictidaceae, which includes a large number of species that are poorly known, since they usually have small fruiting bodies that are rarely collected, and thus, their taxonomy remains partly unresolved. Here, we introduce a new genus Ostropomyces to accommodate a novel lineage related to Ostropa, which is composed of two new species, as well as a new species of Sphaeropezia, S. shangrilaensis. Maximum likelihood and Bayesian inference analyses of mitochondrial small subunit spacers (mtSSU), large subunit nuclear rDNA (LSU), and internal transcribed spacers (ITS) sequence data, together with phenotypic data documented by detailed morphological and anatomical analyses, support the taxonomic affinity of the new taxa in Stictidaceae. Ancestral character state analysis did not resolve the ancestral nutritional status of Stictidaceae with confidence using Bayes traits, but a saprotrophic ancestor was indicated as most likely in a Bayesian binary Markov Chain Monte Carlo sampling (MCMC) approach. Frequent switching in nutritional modes between lineages suggests that lifestyle transition played an important role in the evolution of this family.

Two new genera and twelve new species of Graphidaceae from Puerto Rico: a case for higher endemism of lichenized fungi in islands of the Caribbean?

Phytotaxa ◽  
2014 ◽  
Vol 189 (1) ◽  
pp. 186 ◽  
Author(s):  
JOEL A. MERCADO-DÍAZ ◽  
ROBERT LÜCKING ◽  
SITTIPORN PARNMEN
Keyword(s):  
Puerto Rico ◽  
New Species ◽  
Sequence Data ◽  
New Combination ◽  
New Genera ◽  
Molecular Sequence Data ◽  
Molecular Sequence ◽  
Biodiversity Knowledge ◽  
Three New Species ◽  
High Level

Two new genera and twelve new species of Graphidaceae are described from Puerto Rico. The two new genera, Borinquenotrema and Paratopeliopsis, are based on a combination of molecular sequence data and phenotype characters. Borinquenotrema, with the single new species B. soredicarpum, features rounded ascomata developing beneath and persistently covered with soralia and with an internal anatomy reminescent of Carbacanthographis; it is close to the  tribe Ocellularieae. Paratopeliopsis, including the single new species P. caraibica, resembles a miniature Topeliopsis but differs in the distinctly farinose thallus and the small, brown ascospores; it is not closely related to the latter genus but belongs in tribe Thelotremateae. The other ten new species belong in the genera Acanthotrema, Clandestinotrema, Compositrema, Fissurina, Ocellularia, and Thalloloma. Acanthotrema alboisidiatum is closely related to A. brasilianum but differs in the short, white isidia resembling insect eggs. Clandestinotrema portoricense has a unique ascospore type with a longitudinal septum only in the proximal cell. Compositrema borinquense resembles a species of Stegobolus but belongs in Compositrema based on sequence data, and is characterized by ascomata with a unique columella composed of thick, irregularly radiating strands. The second new species in this genus, C. isidiofarinosum, differs by its ecorticate, farinose thallus with scattered, corticate isidia and by its small ascomata with inconspicuous columella. The three new species of Fissurina all have 3-septate ascospores and are otherwise characterized by an isidiate thallus and stellate, orange-yellow lirellae (F. aurantiacostellata), a verrucose thallus strongly encrusted with calcium oxalate crystals and white, irregularly branched lirellae (F. crystallifera), and myriotremoid ascomata arranged in short lines (F. monilifera). Ocellularia portoricensis belongs in the core group of Ocellularia and differs from O. cavata in the white medulla and the larger ascospores becoming brown, whereas O. vulcanisorediata produces prominent soralia and immersed ascomata with apically carbonized excipulum and columella and small, transversely septate, hyaline ascospores; it is closely related to O. conformalis. Finally, Thalloloma rubromarginatum resembles T. haemographum in the brownish lirellae with bright red margin but differs from that and other species in the corticate thallus and the norstictic acid chemistry. The new combination Ampliotrema rimosum (Hale) Mercado-Díaz, Lücking & Parnmen is also proposed. Considering the current biodiversity knowledge on this family, the high level of endemism observed in other groups of organisms in the island, and the relatively high number of Graphidaceae described, it is highly likely that at least some of these new taxa are endemic to the island. This view is further supported by the unique features of several of the new species, representing novel characters in the corresponding genera.

A review of extant Tudivasum Rosenberg & Petit, 1987 (Neogastropoda: Turbinellidae) and description of three new species from Western Australia

2020 ◽  
Author(s):  
Hugh M Morrison ◽  
Lisa A Kirkendale ◽  
Nerida G Wilson
Keyword(s):  
New Species ◽  
Western Australia ◽  
Sequence Data ◽  
Dna Sequence Data ◽  
The Third ◽  
Extant Species ◽  
Low Diversity ◽  
Three New Species ◽  
Morphological Species ◽  
Shell Sculpture

ABSTRACT Tudivasum Rosenberg & Petit, 1987 is a morphologically distinct gastropod genus of low diversity. All but one species are known from Australia and they occur from the intertidal zone down to hundreds of metres on the continental shelf. These carnivorous gastropods are thought to have intracapsular development. The six currently recognized extant species are reviewed here and their geographical ranges clarified. Two new species, Tudivasum chaneyi n. sp. and T. ashmorense n. sp., are described from Ashmore Reef, Western Australia, and are characterized by differences in protoconch colour and shell sculpture. The third new species, T. westrale n. sp., is described from the mid-west coast of Western Australia, where it has long been misidentified as T. spinosum (H. Adams & A. Adams, 1864). We generated a molecular phylogeny based on mitochondrial DNA sequence data to test morphological species concepts and reconstruct relationships among four of the described species. High levels of divergence within one of the new species could indicate an additional cryptic species.

Three novel species of Russula from southern China based on morphological and molecular evidence

Phytotaxa ◽  
2019 ◽  
Vol 392 (4) ◽  
pp. 264 ◽  
Author(s):  
JING-WEI LI ◽  
JIAN-FEI ZHENG ◽  
YU SONG ◽  
FA YUAN ◽  
LI-HONG QIU
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Novel Species ◽  
Southern China ◽  
Morphological Characters ◽  
Its Sequences ◽  
Molecular Evidence ◽  
Three New Species

Three new species of Russula collected from southern China are proposed based on morphological characters and phylogenetic analysis of internal transcribed spacer (ITS) sequences. Russula bubalina sp. nov. is characterized by cinnamon buff to pink pileus with striate margin, interveined and forked lamellae, basidiospores with warty ornamentations not forming reticulum, hymenial cystidia becoming brown in sulphovanillin (SV) and slender terminal cells in suprapellis. R. pseudobubalina sp. nov. is closely related to Russula bubalina in macro-morphology, but it can be recognized by its unforked lamellae, shorter cheilocystidia, bigger basidia and basidiospores with lower ornamentations. Russula subatropurpurea sp. nov. can be recognized by the purplish brown pileus, mild-tasted and white context changing to light purplish red with FeSO4, white and forking lamellae without lamellulae, long hymenial cystidia becoming brown in SV. Both morphological and phylogenetic analysis consistently confirmed the distinct positions of three new species in subg. Heterophyllidia subsection Heterophyllinae.

Anischia, Perothops and the phylogeny of Elateroidea (Coleoptera: Elateriformia)

2007 ◽  
Vol 38 (2) ◽  
pp. 205-239 ◽  
Author(s):  
Gunilla Ståhls ◽  
Jyrki Muona ◽  
Varpu Vahtera ◽  
Marianna Teräväinen ◽  
John Lawrence
Keyword(s):  
New Species ◽  
Type Species ◽  
New Caledonia ◽  
Sequence Data ◽  
Sensu Stricto ◽  
New Combination ◽  
Larval Morphology ◽  
The Family ◽  
Cladistic Analyses ◽  
Three New Species

AbstractThe larvae of Anischia Fleutiaux and Perothops Laporte are described. Cladistic analyses based on adult and larval morphology, as well as CO1 sequence data, place both genera in the Eucnemidae clade within the Elateroidea (sensu stricto). The subfamily Anischiinae Fleutiaux, 1936 is placed in the family Eucnemidae in a clade containing the more derived eucnemid subfamilies (Melasinae, Eucneminae and Macraulacinae), while Perothops and Phyllocerus Lepeletier & Serville represent subfamilies basal to the remaining eucnemid taxa. The genus Afranischia Basilewsky, 1955 is synonymized with Anischia Fleutiaux, 1896, and Anischia boliviana Fleutiaux is selected as the type species of the latter. Three new species are described: Anischia bicolor (New Caledonia), Anischia kuscheli (New Caledonia) and Anischia monteithi (NE Australia), and Anischia stupenda Fleutiaux, 1897 is recorded from Australia. Anischia crassicornis Champion, 1897 is synonymized with Anischia mexicana Fleutiaux, 1896. One new combination is made, Anischia ruandana (Basilewsky, 1955).

Verrucaria ahtii, V. oulankaensis and V. vitikainenii, three new species from the Endocarpon group (Verrucariaceae, lichenized Ascomycota)

2017 ◽  
Vol 49 (2) ◽  
pp. 107-116 ◽  
Author(s):  
Juha PYKÄLÄ ◽  
Annina LAUNIS ◽  
Leena MYLLYS
Keyword(s):  
New Species ◽  
Sister Group ◽  
Its Sequences ◽  
Its Phylogeny ◽  
Three New Species ◽  
Lichen Flora

AbstractThree new species of Verrucaria are described from calcareous and calciferous rocks in Finland based on morphology and ITS sequences. The species are all members of the Endocarpon group in the Verrucariaceae. Verrucaria oulankaensis sp. nov. is related to V. cernaensis but differing in its usually pruinose, small areolate thallus. It occurs in NE Finland on calcareous and calciferous rocks on river shores. Verrucaria ahtii sp. nov. and V. vitikainenii sp. nov. form a sister group in the ITS phylogeny. Verrucaria vitikainenii differs from V. ahtii in the darker and thinner thallus, absence of a prothallus and in the perithecia, which lack thalline cover. Verrucaria ahtii morphologically resembles V. apomelaena but differs in having a thinner involucrellum and a fimbriate prothallus. It has a southern distribution in Finland and prefers sun-exposed sites, particularly pebbles in lime quarries. The species is also reported from Lithuania and Russia. Verrucaria vitikainenii has an eastern distribution in Finland. The species is a strict calcicole, preferring half-shady habitats. Verrucaria apomelaena is excluded from the Finnish lichen flora.

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