CD169+ macrophages in lymph node and spleen critically depend on dual RANK and LTbetaR signaling

CD169+ macrophages reside in lymph node (LN) and spleen and play an important role in the immune defense against pathogens. As resident macrophages, they are responsive to environmental cues to shape their tissue-specific identity. We have previously shown that LN CD169+ macrophages require RANKL for formation of their niche and their differentiation. Here, we demonstrate that they are also dependent on direct lymphotoxin beta (LTβ) receptor (R) signaling. In the absence or the reduced expression of either RANK or LTβR, their differentiation is perturbed, generating myeloid cells expressing SIGN-R1 in LNs. Conditions of combined haploinsufficiencies of RANK and LTβR revealed that both receptors contribute equally to LN CD169+ macrophage differentiation. In the spleen, the Cd169-directed ablation of either receptor results in a selective loss of marginal metallophilic macrophages (MMMs). Using a RANKL reporter mouse, we identify splenic marginal zone stromal cells as a source of RANKL and demonstrate that it participates in MMM differentiation. The loss of MMMs had no effect on the splenic B cell compartments but compromised viral capture and the expansion of virus-specific CD8+ T cells. Taken together, the data provide evidence that CD169+ macrophage differentiation in LN and spleen requires dual signals from LTβR and RANK with implications for the immune response.

Calling in to Cut back: Settlers learning to listen for a decolonial future

Clearing the gorse, a particularly aggressive invasive plant, so that native plants can flourish has been used as a potent metaphor for decolonization ( Came, 2014 ), and described as labour appropriate for settlers to perform in the interest of just relations with Indigenous peoples. Yet, this labour is not simply one of negation, for it involves learning to bring one’s group difference alongside that of others rather than continuing to mistake that difference for the unmarked context of Indigenous-settler relations. Clearing the gorse is thus also connected to the labour of “gathering at the gate”: the requirement according to Māori protocols of encounter that visitors develop a sense of collective identity and purpose before any meeting can take place. Settler societies, as a rule, operate without a collective sense of the specific identity and history of being a settler people. How would these two forms of labour appropriate for tauiwi Pākehā to perform be connected, and how would performing them together serve broader projects of decolonization and honouring settler commitments in Te Tiriti o Waitangi? I reflect upon this question in light of insights from Tauiwi Tautoko, a recent nationwide anti-racism programme wherein tauiwi (non-Māori settlers) addressed anti-Māori racism online. Core to the programme’s novel anti-racism approach were listening strategies that both invited and modelled acknowledgment of the particular ground from which tauiwi Pākehā see and speak. These strategies have proven effective in creating openings and shifts regarding racist views in otherwise adversarial and toxic spaces. They offer innovative practical resources for the work settlers can and must do with our own people, if we are to contribute to a decolonial future.

Beyond translation into chaos: exploring language movement in the French Caribbean

As Edwin Gentzler’s latest book (2001) reveals, translation studies (as opposed to translating) is an area that is becoming increasingly relevant to both cultural and literary studies. Developing this point further, Sherry Simon states that, “Increasingly, translation and writing have become a particularly strong form of writing at a time when national cultures have themselves become diverse, inhabited by plurality”(Simon 1999: 72). Or indeed how “Symbolically, translation comes to be the very representation of the play of equivalence and difference in cultural interchange: translation permits communication without eliminating the grounds of specificity” (Simon 1992: 159). Therefore, particularly in postcolonial contexts, where the balance of power hinges on questions of language possession and linguistic insecurities, translation allows this power to be repositioned: it can establish a form of plurality by refusing to allow one language to dominate another. In recent works exploring the complex relationship between postcolonial environments and translation,1 these issues are examined in a worldwide context – writings from Quebec, North Africa, India constitute but a few examples. Yet, Simon also draws our attention to processes of translation that allow each language to maintain its own specific identity. In the French Caribbean, this becomes highly problematic because of the tensions between French – the official language – and Creole – the native spoken language.2 This article will explore the difficulties involved in establishing and maintaining this language specificity and will look at how, and if, French and Creole can ‘translate ’French Caribbean culture.

Secure Outsourced Attribute-Based Signatures with Perfect Anonymity in the Standard Model

Outsourced attribute-based signatures (OABS) enable users to sign messages without revealing specific identity information and are suitable for scenarios with limited computing power. Recently, Mo et al. proposed an expressive outsourced attribute-based signature scheme (Peer-to-Peer Networking and Applications, 11, 2017). In this paper, we show that Mo et al.’s scheme does not achieve any of the three security properties. Their scheme is incorrect. The adversary can collude with the malicious signing-cloud service provider (S-CSP) to forge valid signatures on any message and any attribute set. And the S-CSP could trace the access structures used to generate the signatures. Then, we treat the S-CSP as an adversary and present more accurate unforgeability and anonymity models for OABS to remedy the drawbacks of the previous ones. Finally, we propose a simple but significant improvement to fix our attacks. The improved scheme achieves correctness, unforgeability, and perfect anonymity while keeping the efficiency almost unchanged. We also prove the security of the improved scheme under the standard model.

Occurrence of Neobenedenia girellae (Monogenea: Capsalidae) in Gilthead Seabream Sparus aurata (Actinopterygii: Sparidae) Cultured in Portugal

Monogenean capsalids of the genus Neobenedenia are widespread parasites of wild and farmed marine fish, and represent a potential threat to mariculture due to their pathogenicity and ability to cause mortality in fish maintained in controlled conditions. The identification of Neobenedenia species and, consequently, the definition of their host specificity is often problematic due to their highly conserved morphology; therefore, in order to establish their specific identity, microscopic observation should be complemented with molecular analysis. The present work aims at characterizing Neobenedenia specimens infecting the skin of cage reared gilthead seabream Sparus aurata from Portugal. Parasite samples obtained from caged fish were processed for morphological analysis, through observation in light and scanning electron microscopy, and for molecular analysis, through amplification and sequencing of 28S rDNA and cytB, aimed at identifying them to the species level. Our results showed that the collected parasites belonged to the species Neobenedenia girellae; the susceptibility of S. aurata towards this pathogenic capsalid monogenean highlighted in the present work represents an important risk in the farming of this valuable fish species.

Who tweets climate change papers? Investigating publics of research through users’ descriptions

As social issues like climate change become increasingly salient, digital traces left by scholarly documents can be used to assess the resonance of scientific knowledge outside academia. Our research describes a method to assess the publics of research on Twitter by focusing on perceived users who shared highly tweeted climate change papers. It examines users through eight categories (academia, communication, political, professional, personal, organization, bots and publishers) associated to specific expressions in Twitter profile descriptions. Results indicate how diverse publics may be represented in the communication of scholarly documents on Twitter. Supplementing our word detection analysis with qualitative assessments of the results, we highlight how the presence of unique or multiple categorizations in textual Twitter descriptions provides evidence of the publics of research in specific contexts. The notion of perceived users allows to circumvent some issues about the construction of profiles through specific identity markers. Furthermore, the flexibility of our method provide means for research assessment that take into account the contextuality and plurality of publics involved on Twitter.

Well-prefaced constructed dialogue as a marker of stance in online abortion discourse

This paper offers an analysis of well-prefaced constructed dialogue as a stance-taking resource in written discourse on abortion. Drawing from four corpora collected from editorials, blogs, Twitter, and Reddit, I demonstrate that writers use the discourse marker well to indicate a stance of disalignment and convey negative attitudinal information when there is tension between the writer’s beliefs and those expressed in the constructed dialogue; the discourse marker allows the writer to position and align themself to construct a specific identity that reinforces a positive-self, negative-other evaluation.

Public discourses about work: narrative sources with a high pedagogical-social value

Is there a work and organizations’ethnography and anthropology that has passed and passes through words and discourses? Is it possible to extract from the analysis of public discourse about work the narration to outline an idea of work not only set up and carried out but also told, imagined, desired and above all educating? Printed paper, educational, scientific and political production can be considered very important sources to build a discourse about work and “popular pedagogy” and common sense connected to it in the sense of narration as historical and metabletic tool that contributes to individual and collective development. Words and speeches are epistemological frames and glances at the world have a connection with things. The word is not only a sign, it is also an image. The word brings, somewhat enigmatic, a connection with what it represents. Words therefore also have the power to produce transformations, to change the world, the way they see it and to represent it. Foucault had also written that words are fundamental codes behind a culture and influence experience and thought. Words and speeches about work, spoken and written, then, are a meta-narrative and can help to draw and dissect an unprecedented, alternative, parallel story. Also, a history of pedagogy of work is full of theories, models, methods but also of stories, of speeches of intellectuals, trade unionists, scholars, prominent figures, entrepreneurs who have dedicated their lives to the cause of a dignified and good work. For example, the speeches of Vittorio, Adriano Olivetti, the encyclicals of the different popes that have succeeded each other in history, the many aphorisms and speeches made by great entrepreneurs such as Cucinelli, Steve Jobs etc. that, in different ways, have helped to build an idea of work that - over the centuries - has profoundly changed. Starting from these reflections, the contribution aims to highlight the baggage of intangible assets and the implicit educational deposited in some exemplary narrative passages on the work that have helped to build collective stories and produce a shared sense, to give a specific identity to work and to propose its widespread representation with a high pedagogical-social value.