Characterization of the complete mitochondrial genome of Setaria digitata (Nematoda: Setariidae) from China

2017 ◽  
Vol 91 (6) ◽  
pp. 772-776 ◽  
Author(s):  
G.-H. Liu ◽  
J.-Y. Li ◽  
X.-Q. Zhu
Keyword(s):  
Population Genetics ◽  
Amino Acid ◽  
Sri Lanka ◽  
Genome Sequence ◽  
Complete Mitochondrial Genome ◽  
Economic Losses ◽  
Setaria Digitata ◽  
Livestock Farmers ◽  
Mt Genome

AbstractSetaria digitata is a filarial parasite that causes fatal cerebrospinal nematodiasis in goats, horses and sheep, resulting in substantial economic losses to livestock farmers. In the present study, the complete mitochondrial (mt) genome of S. digitata from China was determined, characterized and compared with that of S. digitata from Sri Lanka. The identity of the mt genomes was 98.3% between S. digitata from China and Sri Lanka, and the complete mt genome sequence of S. digitata from China was slightly shorter (25 bp) than that from Sri Lanka. For the 12 protein genes, this comparison revealed sequence differences at both the nucleotide (1.4%) and amino acid (2.2%) levels. The present study determined the complete mt genome sequence of S. digitata from China, providing novel genetic markers for the study of the population genetics and molecular epidemiology of S. digitata in animals.

Characterization of the complete mitochondrial genome of Ortleppascaris sinensis (Nematoda: Heterocheilidae) and comparative mitogenomic analysis of eighteen Ascaridida nematodes

2017 ◽  
Vol 92 (3) ◽  
pp. 369-378 ◽  
Author(s):  
J.H. Zhao ◽  
G.J. Tu ◽  
X.B. Wu ◽  
C.P. Li
Keyword(s):  
Mitochondrial Genome ◽  
Genome Sequence ◽  
Complete Mitochondrial Genome ◽  
Molecular Ecology ◽  
Protein Coding ◽  
Intestinal Nematode ◽  
The Family ◽  
Polymerase Chain ◽  
Mt Genome

AbstractOrtleppascaris sinensis (Nematoda: Ascaridida) is a dominant intestinal nematode of the captive Chinese alligator. However, the epidemiology, molecular ecology and population genetics of this parasite remain largely unexplored. In this study, the complete mitochondrial (mt) genome sequence of O. sinensis was first determined using a polymerase chain reaction (PCR)-based primer-walking strategy, and this is also the first sequencing of the complete mitochondrial genome of a member of the genus Ortleppascaris. The circular mitochondrial genome (13,828 bp) of O. sinensis contained 12 protein-coding, 22 transfer RNA and 2 ribosomal RNA genes, but lacked the ATP synthetase subunit 8 gene. Finally, phylogenetic analysis of mtDNAs indicated that the genus Ortleppascaris should be attributed to the family Heterocheilidae. It is necessary to sequence more mtNDAs of Ortleppascaris nematodes in the future to test and confirm our conclusion. The complete mitochondrial genome sequence of O. sinensis reported here should contribute to molecular diagnosis, epidemiological investigations and ecological studies of O. sinensis and other related Ascaridida nematodes.

Insect mitochondrial genomics 2: the complete mitochondrial genome sequence of a giant stonefly, Pteronarcys princeps, asymmetric directional mutation bias, and conserved plecopteran A+T-region elements

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 815-824 ◽  
Author(s):  
James Bruce Stewart ◽  
Andrew T Beckenbach
Keyword(s):  
Genome Sequence ◽  
Genome Rearrangement ◽  
Complete Mitochondrial Genome ◽  
Nucleotide Composition ◽  
Genome Sequences ◽  
Mutation Pressure ◽  
Stem Loop ◽  
Mitochondrial Genomics ◽  
Directional Mutation Pressure ◽  
Mt Genome

Mitochondrial (mt) genome sequences of insects are receiving renewed attention in molecular phylogentic studies, studies of mt-genome rearrangement, and other unusual molecular phenomena, such as translational frameshifting. At present, the basal neopteran lineages are poorly represented by mt-genome sequences. Complete mt-genome sequences are available in the databases for only the Orthoptera and Blatteria; 9 orders are unrepresented. Here, we present the complete mt-genome sequence of a giant stonefly, Pteronarcys princeps (Plecoptera; Pteronarcyidae). The 16 004 bp genome is typical in its genome content, gene organisation, and nucleotide composition. The genome shows evidence of strand-specific mutational biases, correlated with the time between the initiation of leading and the initiation of lagging strand replication. Comparisons with other insects reveal that this trend is seen in other insect groups, but is not universally consistent among sampled mt-genomes. The A+T region is compared with that of 2 stoneflies in the family Peltoperlidae. Conserved stem-loop structures and sequence blocks are noted between these distantly related families.Key words: mitochondrial genomics, directional mutation pressure, A+T-rich region, Plecoptera, stonefly.

scholarly journals Characterization of the Complete Mitochondrial Genome of Ostertagia trifurcata of Small Ruminants and its Phylogenetic Associations for the Trichostrongyloidea Superfamily

Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 107 ◽  
Author(s):  
Awais Ahmad ◽  
Xin Yang ◽  
Ting Zhang ◽  
Chunqun Wang ◽  
Caixian Zhou ◽  
...  
Keyword(s):  
Amino Acid ◽  
Complete Mitochondrial Genome ◽  
Small Ruminants ◽  
Complete Characterization ◽  
Amino Acid Sequences ◽  
Trna Genes ◽  
Protein Coding ◽  
Coding Regions ◽  
Mtdna Sequence ◽  
Mt Genome

The complete mitochondrial (mt) genome of Ostertagia trifurcata, a parasitic nematode of small ruminants, has been sequenced and its phylogenetic relationship with selected members from the superfamily Trichostrongyloidea was investigated on the basis of deduced datasets of mt amino acid sequences. The entire mt genome of Ostertagia trifurcata is circular and 14,151 bp in length. It consists of a total of 36 genes comprising 12 genes coding for proteins (PCGs), 2 genes for ribosomal RNA (rRNA), 22 transfer RNA (tRNA) genes and 2 non-coding regions, since all genes are transcribed in the same direction. The phylogenetic analysis based on the concatenated datasets of predicted amino acid sequences of the 12 protein coding genes supported monophylies of the Haemonchidae, Dictyocaulidae and Molineidae families, but rejected monophylies of the Trichostrongylidae family. The complete characterization and provision of the mtDNA sequence of Ostertagia trifurcata provides novel genetic markers for molecular epidemiological investigations, systematics, diagnostics and population genetics of Ostertagia trifurcata and its correspondents.

Characterization of Clarias gariepinus mitochondrial genome sequence and a comparative analysis with other catfishes

Biologia ◽  
2015 ◽  
Vol 70 (9) ◽  
pp. 1245-1253
Author(s):  
Chong Han ◽  
Qiang Li ◽  
Junqiu Xu ◽  
Xiufeng Li ◽  
Jianrong Huang
Keyword(s):  
Mitochondrial Genome ◽  
Control Region ◽  
Genome Sequence ◽  
Complete Mitochondrial Genome ◽  
Clarias Gariepinus ◽  
African Catfish ◽  
Protein Coding ◽  
Mitochondrial Genome Sequence ◽  
Complete Mitochondrial Genome Sequence

Abstract Clarias gariepinus is an economically important fish belonging to Siluriformes. In order to understand the characterization of catfishes, the complete mitochondrial genome sequence of the African catfish C. gariepinus was determined using 17 pairs of primers. The complete mitogenome is 16,508 bp in length and contains 37 mitochondrial genes (13 protein-coding, two rRNA, and 22 tRNA genes) and a control region as in most other fishes. Moreover, a comparison of C. gariepinus with Clarias batrachus, Clarias fuscus and Clarias sp. NM-2010 indicated that the four mitogenomics were similar in length, total nucleotide compositions and codon usage patterns, but different in 13 protein-coding genes, control region and other aspects. The complete mitochondrial genome sequence of C. gariepinus would contribute to better understanding of population genetics, conservation, biogeography and evolution of this species.

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