scholarly journals The influence of mineral carriers on the simultaneous active and passive immunization of guinea-pigs against tetanus

1965 ◽  
Vol 63 (2) ◽  
pp. 243-262 ◽  
Author(s):  
A. J. Fulthorpe
Keyword(s):  
Calcium Phosphate ◽  
Tetanus Toxoid ◽  
Aluminium Hydroxide ◽  
Guinea Pigs ◽  
Calcium Salt ◽  
Primary Response ◽  
Tissue Response ◽  
Aluminium Phosphate ◽  
Tetanus Antitoxin ◽  
Significant Difference

Guinea-pigs given two doses of 2–25 Lf of fluid tetanus toxoid at 28 days interval had very satisfactory antitoxin titres 10 days after the second dose of toxoid (g.m. 28–2 units/ml.). Similar groups of animals given 150 units of horse tetanus antitoxin simultaneously with the first dose of toxoid responded very badly (g.m. < 0·016).Interference by passive antitoxin occurred even when the antitoxin was given as late as 4 days after the first dose of toxoid.Interference by passively administered antitoxin was minimal when aluminium hydroxide-adsorbed toxoid was used. It was necessary to increase the dose of antitoxin from 150 to 2400 units before significant interference occurred.The route of administration of antitoxin did not significantly affect the results except when the antitoxin was given intravenously.When guinea-pigs were immunized and bled at regular intervals it was found that with both fluid and aluminium hydroxide-adsorbed preparations, titratable antitoxin was present on the 14th day. The increase in titre thereafter was more rapid with the adsorbed preparation, but after a second dose of toxoid there was no significant difference in titre.Passively administered antitoxin virtually abolished the active response to fluid toxoid, but with aluminium hydroxide-adsorbed preparations the primary response was not abolished but reduced and delayed and there was much individual variation.Horse serum-sensitive guinea-pigs given adsorbed toxoid with simultaneous passive horse antitoxin gave a better primary response to the toxoid than did unsensitive animals.The effectiveness of adsorbed tetanus toxoid in the simultaneous immunization procedure was directly related to the concentration of aluminium hydroxide or phosphate used: this concentration was critical and amounts below a certain level were ineffective. Calcium phosphate used as an adsorbent was unsatisfactory in this way, although it was an excellent adsorbent.Investigation of the adsorbent characteristics of aluminium hydroxide and phosphate and of calcium phosphate, showed that the calcium salt on a molar basis was the most effective and that aluminium phosphate was the least effective.Elution of toxoid from centrifuged precipitates of the three types of adsorbent showed that only 5% of toxoid was removed from the aluminium hydroxide, 13–18% from the calcium phosphate and 31–33% from the aluminium phosphate preparation when incubated with normal serum at 37° C.Aluminium hydroxide adsorption in vitro interfered with the ability of antitoxin to combine with toxoid and to a lesser extent calcium phosphate had the same effect; aluminium phosphate, however, did not appear to interfere at all in this wayHistological observations on the tissue response to aluminium phosphate and calcium phosphate indicated that the typical alum granuloma produced by aluminium phosphate was not produced by the calcium salt.

scholarly journals Age- and sex-determined differences in the establishment of tetanus antitoxin production in guinea-pigs

2001 ◽  
Vol 49 (1) ◽  
pp. 25-30
Author(s):  
L. A. Réthy ◽  
Magdolna Géresi ◽  
L. Réthy
Keyword(s):  
Tetanus Toxoid ◽  
Guinea Pigs ◽  
Sex Chromosome ◽  
Age Groups ◽  
Immune Memory ◽  
Aluminium Phosphate ◽  
Sexual Hormones ◽  
Protein Nitrogen ◽  
Tetanus Antitoxin ◽  
Tetanus Immunisation

For lack of relevant data of the literature, the tetanus immunisation results obtained in the two sexes were compared in an animal model. Complete immunisation series of weaned, adult and aged guinea-pigs (20–25 animals/group) were performed with aluminium phosphate (AlPO4) adsorbed purified tetanus toxoid (PTAP) as well as with typhoid-tetanus vaccine (TY-TE) containing lipopolysaccharide (LPS). Both vaccines contained 5.0 Lf (limes flocculans, Ramon) per single dose of tetanus toxoid, purity degree: 1500 Lf/mg protein nitrogen (PN). Tetanus antitoxin titres (TAT) were measured after the first shot, and subsequently before and after booster. Compared to TAT of male animals, significantly lower titres were found in female animals after basic immunisation with PTAP in all the three age groups: 1.03 vs. 0.57, 8.75 vs. 5.64, and 0.27 vs. 0.15 IU (international units, related to the Copenhagen International Standard) per ml (sex-chromosome-dependent differences?), as well as in adult animals immunised with TY-TE, before booster: 0.07 vs. 0.02 IU/ml (hormone-dependent differences?). In the latter case the TAT results after booster were 14.49 vs. 12.89 IU/ml. Thus, the lower female prebooster titres were counterbalanced by a quick and effective increase of titres following booster. These results are in accordance with our previous observations in humans (Réthy and Réthy, 1986). From our observations with tetanus immunisation series on guinea-pigs it can be concluded that TAT may be influenced by the effects of sex chromosomes as well as of sexual hormones. During active anti-tetanus immunisation with LPS-containing vaccine (TY-TE) the lower adult female prebooster titres are presumably counterbalanced by the better functionality of the female immune memory.

scholarly journals Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs

2013 ◽  
Vol 9 (3) ◽  
pp. 5464-5474 ◽  
Author(s):  
Matilde Bongio ◽  
Jeroen J.J. van den Beucken ◽  
M. Reza Nejadnik ◽  
Zeinab Tahmasebi Birgani ◽  
Pamela Habibovic ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Guinea Pigs ◽  
Subcutaneous Tissue ◽  
Medullary Cavity ◽  
Tissue Response ◽  
Calcium Phosphate Nanoparticle

scholarly journals Simultaneous active and passive immunization of guinea-pigs against tetanus

1964 ◽  
Vol 62 (3) ◽  
pp. 379-388 ◽  
Author(s):  
J. W. G. Smith
Keyword(s):  
Tetanus Toxoid ◽  
Aluminium Hydroxide ◽  
Guinea Pigs ◽  
Horse Serum ◽  
Passive Immunization ◽  
Better Than

1. Simultaneous active and passive immunization of guinea-pigs using tetanus toxoid adsorbed on to aluminium hydroxide produced a higher antitoxin response than simultaneous immunization using plain toxoid. The response was also better than that produced by plain toxoid alone.2. A 24 hr. interval between the injection of plain toxoid and the subsequent injection of antitoxin gave no better results than when toxoid and antitoxin were given at the same time.3. The response to simultaneous immunization, using adsorbed toxoid, of guinea-pigs sensitized to horse serum, was superior in the first 6 weeks to that of guinea-pigs which had not been sensitized.4. The suggestion that simultaneous active and passive immunization of man should be performed with adsorbed toxoid rather than plain toxoid is supported by these results.

STUDIES OF THE IMMUNOLOGY OF THE NEWBORN INFANT

PEDIATRICS ◽  
1952 ◽  
Vol 9 (6) ◽  
pp. 736-744
Author(s):  
JOHN J. OSBORN ◽  
JOSEPH DANCIS ◽  
JUAN F. JULIA
Keyword(s):  
Single Dose ◽  
Tetanus Toxoid ◽  
Early Life ◽  
Statistical Tests ◽  
High Titer ◽  
Diphtheria Toxoid ◽  
Tetanus Antitoxin ◽  
Significant Difference ◽  
Tetanus Immunization ◽  
Better Than

Forty infants from 1 week to 6 months of age who had no circulating antitoxin were immunized with one injection of a high titer diphtheria toxoid on alum and their antitoxin responses were followed at regular intervals up to six months afterwards. The ability to form antitoxin improved significantly during the first two months of life. No significant difference could be demonstrated between the response of infants 2 to 4 months of age and that of infants 6 months of age. (In the statistical tests, the 5% level was adopted as indicating significance.) Twenty-seven of these infants were immunized at the same time in the opposite arm with a single dose of tetanus toxoid on alum. The response to tetanus immunization was faster and better in infants from 2 to 6 months of age than in infants 1 month of age and younger. The response to the tetanus toxoid preparation was faster and better than the response to the diphtheria toxoid preparation. The following conclusions are drawn: A. The infant can form antibodies from birth. B. The ability to form antibodies improves during early life. For diphtheria and tetanus antitoxin, the improvement is rapid during the first two months and then is much slower. C. The advisability of early immunization is related to the potency of the antigen to be used.

scholarly journals Prospective study to assess the tissue response to HPC-coated p48 flow diverter stents compared to uncoated devices in the rabbit carotid artery model

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Tim Lenz-Habijan ◽  
Pervinder Bhogal ◽  
Catrin Bannewitz ◽  
Ralf Hannes ◽  
Hermann Monstadt ◽  
...  
Keyword(s):  
Foreign Body ◽  
Flow Diverter ◽  
Foreign Body Response ◽  
Tissue Response ◽  
Acute Setting ◽  
Significant Difference ◽  
And Performance ◽  
Surface Modified ◽  
Common Carotid Arteries

Abstract Background Flow diverters (FDs) are widely used in the treatment of intracranial aneurysms, but the required medication increases the risk of haemorrhagic complications and limits their use in the acute setting. Surface modified FDs may limit the need for dual antiplatelet therapy (DAPT). Hydrophilic polymer coating (HPC) may reduce the need of medication. Methods This explorative study, approved by the local authorities and the local welfare committee, compared stent behaviour and overall tissue response between HPC-coated FDs and uncoated FDs, both implanted into the common carotid arteries of eight New Zealand white rabbits. Endothelialisation, inflammatory response, and performance during implantation were assessed. Angiographic follow-up was performed to observe the patency of the devices after implantation and after 30 days. Histological examinations were performed at 30 days to assess foreign body reaction and endothelialisation. Kruskal-Wallis and Wilcoxon tests were used to compare non-parametric variables. Results Angiography showed that both coated and uncoated FDs performed well during implantation. All devices remained patent during immediate follow-up and after 30 days. Histopathology showed no significant difference in inflammation within the vessel wall between the two cohorts (2.12 ± 0.75 vs. 1.96 ± 0.79, p = 0.7072). Complete endothelialisation of the stent struts was seen with very similar (0.04 ± 0.02 mm vs. 0.04 ± 0.03 mm, p = 0.892) neoendothelial thickness between the two cohorts after 30 days. Conclusion Taking into account the limitation in sample size, non-significant differences between the HPC-coated and uncoated FDs regarding implantation, foreign body response, and endothelialisation were found.

scholarly journals The Peptide Vaccine Combined with Prior Immunization of a Conventional Diphtheria-Tetanus Toxoid Vaccine Induced AmyloidβBinding Antibodies on Cynomolgus Monkeys and Guinea Pigs

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Akira Yano ◽  
Kaori Ito ◽  
Yoshikatsu Miwa ◽  
Yoshito Kanazawa ◽  
Akiko Chiba ◽  
...  
Keyword(s):  
Immune Responses ◽  
Tetanus Toxoid ◽  
Guinea Pigs ◽  
Peptide Vaccine ◽  
Cell Epitope ◽  
T Cell Epitope ◽  
Peptide Vaccination ◽  
Binding Profile ◽  
Cynomolgus Monkeys ◽  
The Brain

The reduction of brain amyloid beta (Aβ) peptides by anti-Aβantibodies is one of the possible therapies for Alzheimer’s disease. We previously reported that the Aβpeptide vaccine including the T-cell epitope of diphtheria-tetanus combined toxoid (DT) induced anti-Aβantibodies, and the prior immunization with conventional DT vaccine enhanced the immunogenicity of the peptide. Cynomolgus monkeys were given the peptide vaccine subcutaneously in combination with the prior DT vaccination. Vaccination with a similar regimen was also performed on guinea pigs. The peptide vaccine induced anti-Aβantibodies in cynomolgus monkeys and guinea pigs without chemical adjuvants, and excessive immune responses were not observed. Those antibodies could preferentially recognize Aβ40, and Aβ42compared to Aβfibrils. The levels of serum anti-Aβantibodies and plasma Aβpeptides increased in both animals and decreased the brain Aβ40level of guinea pigs. The peptide vaccine could induce a similar binding profile of anti-Aβantibodies in cynomolgus monkeys and guinea pigs. The peptide vaccination could be expected to reduce the brain Aβpeptides and their toxic effects via clearance of Aβpeptides by generated antibodies.

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