Quinone reductase activity is widespread in lichens

AbstractIn our earlier work, we demonstrated that the oxidases tyrosinase (TYR), laccase (LAC), and a heme peroxidase (POX) occur widely in lichens. Here we report on the occurrence of another oxidoreductase enzyme, quinone reductase (QR) (EC 1.6.5.5). While QR has been reported to occur widely in other organisms, there is currently no information on QR activities in lichens. Here we present a survey of QR activity in 14 species of lichens. Results demonstrate that QR activity is readily detectable in all lichen species tested. However, activities vary greatly, with ‘jelly’ lichens in the genera Collema and Leptogium having the highest activities. QR, LAC and POX are all believed to have a role in extracellular hydroxyl radical production. However, in this study no correlation was found between the activities of these enzymes and the rates at which hydroxyl radicals were produced. Possible roles for QR in lichen biology are discussed.

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Hydroxyl radical production induced by plasma hydrogenated nanodiamonds under X-ray irradiation

Chemical Communications ◽

10.1039/c6cc08895c ◽

2017 ◽

Vol 53 (7) ◽

pp. 1237-1240 ◽

Cited By ~ 14

Author(s):

Magdalena Kurzyp ◽

Hugues A. Girard ◽

Yannis Cheref ◽

Emilie Brun ◽

Cecile Sicard-Roselli ◽

...

Keyword(s):

Hydroxyl Radical ◽

Hydroxyl Radicals ◽

Detonation Nanodiamonds ◽

X Ray ◽

Radical Production ◽

First Time

For the first time, overproduction of hydroxyl radicals (HO˙) induced by plasma hydrogenated detonation nanodiamonds (H-NDs) under X-ray irradiation is reported.

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Intrinsic Buffer Hydroxyl Radical Dosimetry Using Tris(Hydroxymethyl)Aminomethane

10.1101/825463 ◽

2019 ◽

Author(s):

Addison E. Roush ◽

Mohammad Riaz ◽

Sandeep K. Misra ◽

Scot R. Weinberger ◽

Joshua S. Sharp

Keyword(s):

Hydroxyl Radical ◽

Real Time ◽

Hydroxyl Radicals ◽

Flash Photolysis ◽

Laser Flash Photolysis ◽

Laser Flash ◽

Photochemical Oxidation ◽

Protein Surfaces ◽

Radical Production ◽

Experimental Parameters

AbstractFast Photochemical Oxidation of Proteins (FPOP) is a powerful covalent labeling tool that uses hydroxyl radicals generated by laser flash photolysis of hydrogen peroxide to footprint protein surfaces. Because radical production varies with many experimental parameters, hydroxyl radical dosimeters have been introduced to track the effective radical dosage experienced by the protein analyte. FPOP experiments performed using adenine optical radical dosimetry containing protein in Tris buffer demonstrated unusual dosimetry behavior. We have investigated the behavior of Tris under oxidative conditions in detail. We find that Tris can act as a novel gain-of-signal optical hydroxyl radical dosimeter in FPOP experiments. This new dosimeter is also amenable to inline real-time monitoring thereby allowing real-time adjustments to compensate for differences in samples for their quenching ability.

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The Production of Hydroxyl Radicals in the Fetal Lamb Brain Resulting from Occlusion of the Umbilical Circulation and the Transplacental Effect of MCI-186 to Inhibit Hydroxyl Radical Production

Pediatric Research ◽

10.1203/01.pdr.0000196373.52086.c2 ◽

2006 ◽

Vol 59 (2) ◽

pp. 216-220 ◽

Cited By ~ 3

Author(s):

Yoshiyuki Nakajima ◽

Naoki Masaoka ◽

Yasuhito Hayakawa ◽

Masao Watanabe ◽

Masaji Nagaishi ◽

...

Keyword(s):

Hydroxyl Radical ◽

Hydroxyl Radicals ◽

Radical Production ◽

Fetal Lamb

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Chromate tolerance caused by reduced hydroxyl radical production and decreased glutathione reductase activity in Schizosaccharomyces pombe

Journal of Basic Microbiology ◽

10.1002/jobm.200390018 ◽

2003 ◽

Vol 43 (2) ◽

pp. 96-103 ◽

Cited By ~ 19

Author(s):

Zoltán Gazdag ◽

István Pócsi ◽

József Belágyi ◽

Tamás Emri ◽

Ágnes Blaskó ◽

...

Keyword(s):

Hydroxyl Radical ◽

Schizosaccharomyces Pombe ◽

Glutathione Reductase ◽

Reductase Activity ◽

Radical Production ◽

Glutathione Reductase Activity ◽

Chromate Tolerance

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Enhancement of hydroxyl radical generation by phenols and their reaction intermediates during ozonation

Water Science & Technology ◽

10.2166/wst.1998.0247 ◽

1998 ◽

Vol 38 (6) ◽

pp. 147-154 ◽

Cited By ~ 2

Author(s):

Hideo Utsumi ◽

Sang-Kuk Han ◽

Kazuhiro Ichikawa

Keyword(s):

Hydroxyl Radical ◽

Hydroxyl Radicals ◽

Spin Trapping ◽

Active Species ◽

Generation Rate ◽

Peak Height Ratio ◽

Dependent Manner ◽

Concentration Dependent Manner ◽

Phenol Derivatives ◽

Semiquinone Radicals

Generation of hydroxyl radicals, one of the major active species in ozonation of water was directly observed with a spin-trapping/electron spin resonance (ESR) technique using 5,5-dimethyl-1-pyrrolineN-oxide (DMPO) as a spin-trapping reagent. Hydroxyl radical were trapped with DMPO as a stable radical, DMPO-OH. Eighty μM of ozone produced 1.08 X 10-6M of DMPO-OH, indicating that 1.4% of •OH is trapped with DMPO. Generation rate of DMPO-OH was determined by ESR/stopped-flow measurement. Phenol derivatives increased the amount and generation rate of DMPO-OH, indicating that phenol derivatives enhance •OH generation during ozonation of water. Ozonation of 2,3-, 2,5-, 2,6-dichlorophenol gave an ESR spectra of triplet lines whose peak height ratio were 1:2:1. ESR parameters of the triplet lines agreed with those of the corresponding dichloro-psemiquinone radical. Ozonation of 2,4,5- and 2,4,6-trichlorophenol gave the same spectra as those of 2,5- and 2,6-dichlorophenol, respectively, indicating that a chlorine group in p-position is substituted with a hydroxy group during ozonation. Amounts of the radical increased in an ozone-concentration dependent manner and were inhibited by addition of hydroxyl radical scavengers. These results suggest that p-semiquinone radicals are generated from the chlorophenols by hydroxyl radicals during ozonation. The p-semiquinone radicals were at least partly responsible for enhancements of DMPO-OH generation.

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Humic acids promote hydroxyl radical production during transformation of biogenic and abiogenic goethite under redox fluctuation

Chemical Engineering Journal ◽

10.1016/j.cej.2021.130359 ◽

2021 ◽

pp. 130359

Author(s):

Huali Yu ◽

Guangfei Liu ◽

Bin Dong ◽

Ruofei Jin ◽

Jiti Zhou

Keyword(s):

Hydroxyl Radical ◽

Humic Acids ◽

Radical Production

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Svalbamides A and B, Pyrrolidinone-Bearing Lipodipeptides from Arctic Paenibacillus sp.

Marine Drugs ◽

10.3390/md19040229 ◽

2021 ◽

Vol 19 (4) ◽

pp. 229

Author(s):

Young Eun Du ◽

Eun Seo Bae ◽

Yeonjung Lim ◽

Jang-Cheon Cho ◽

Sang-Jip Nam ◽

...

Keyword(s):

Reductase Activity ◽

Quinone Reductase ◽

The Arctic ◽

Amino Acid Residues ◽

Chemopreventive Agents ◽

Svalbard Archipelago ◽

Paenibacillus Sp ◽

Combinational Analysis ◽

Culture Extract ◽

The Absolute

Two new secondary metabolites, svalbamides A (1) and B (2), were isolated from a culture extract of Paenibacillus sp. SVB7 that was isolated from surface sediment from a core (HH17-1085) taken in the Svalbard archipelago in the Arctic Ocean. The combinational analysis of HR-MS and NMR spectroscopic data revealed the structures of 1 and 2 as being lipopeptides bearing 3-amino-2-pyrrolidinone, d-valine, and 3-hydroxy-8-methyldecanoic acid. The absolute configurations of the amino acid residues in svalbamides A and B were determined using the advanced Marfey’s method, in which the hydrolysates of 1 and 2 were derivatized with l- and d- forms of 1-fluoro-2,4-dinitrophenyl-5-alanine amide (FDAA). The absolute configurations of 1 and 2 were completely assigned by deducing the stereochemistry of 3-hydroxy-8-methyldecanoic acid based on DP4 calculations. Svalbamides A and B induced quinone reductase activity in Hepa1c1c7 murine hepatoma cells, indicating that they represent chemotypes with a potential for functioning as chemopreventive agents.

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