A study on the structure of the whiting integument (Merlangius merlangus L.)

1976 ◽  
Vol 56 (1) ◽  
pp. 213-226 ◽  
Author(s):  
Alistair M. Bullock ◽  
Ronald J. Roberts ◽  
John D. M. Gordon
Keyword(s):  
Alkaline Phosphatase ◽  
Mucous Cell ◽  
Succinic Dehydrogenase ◽  
The Body ◽  
Adult Fish ◽  
Succinic Dehydrogenase Activity ◽  
Acid Component ◽  
Merlangius Merlangus ◽  
Mature Fish ◽  
Alkaline Phosphatase Reaction

The histology and histochemistry of whiting skin was studied in larval, juvenile and adult fish. The adult specimens were obtained throughout the year but there was no evidence of seasonal variation in the areas of the body sampled. The skin of the whiting is considerably more complex than that of any other teleost fish which has been described in detail. The epidermis of the mature fish comprises five layers, consisting of Malpighian cells and a variety of other structures including large cystic bullae, which ultrastructurally and histochemically are relatively amorphous but comprise a major proportion of the epidermis. A reticulum of epidermal melanocytes occurs above these structures and two types of mucous cell are described. The major histochemical feature of the epidermis was the alkaline phosphatase reaction, similar to that found in higher animals. Succinic dehydrogenase activity was found at all levels. The epithelial mucins were mainly acid mucopolysaccharide with a small sialic acid component and a greater proportion of sulphated muco-substance. The dermis was morphologically similar to that of other scaled fishes. Mast cells were particularly prominent and occasional non-pigmented ‘melanophores’ were seen.

Macroscopic, Histomorphological and Histochemical Studies on the Kidneys of Guinea Fowl (Numida meleagris)

2021 ◽  
Author(s):  
Gajendra Singh ◽  
Balwant Meshram ◽  
Hemant Joshi
Keyword(s):  
Alkaline Phosphatase ◽  
Basement Membrane ◽  
Mesangial Cells ◽  
Periodic Acid ◽  
Succinic Dehydrogenase ◽  
Guinea Fowl ◽  
The Body ◽  
Staining Procedure ◽  
Numida Meleagris ◽  
Intense Activity

Background: Kidneys, the paired organ which essentially plays for excreting nitrogenous wastes, excessive water, inorganic salts and toxic substances produced during the process of body metabolism. Maintenance of osmotic regulation and homeostatic fluid balance of the body has also be performed by kidneys. Each of the kidneys filtrate carried to cloaca by respective ureter from where the urine leaves the body. The present macroscopic, histomorphological and histochemical studies undertaken on the Kidneys of Guinea fowl (Numida meleagris) is nothing but the attempt to widen our thrust area for that bird which is nonexistent as on documentation.Methods: The present studies were carried upon 12 pairs of in-vitro kidney samples in Guinea fowl. Gross morphology and morphometrical observations were carried on fresh specimens. Histological studies were carried on fixed samples those were processed for dehydration, clearing, embedding into paraffin and sections of 5-6 μ thickness were obtained. Different staining procedure as to Haematoxylin and Eosin, Silver orcein, Aniline blue and Weigert’s staining methods were employed for different histological components. The histochemical and histoenzymic studies were also accomplished with Periodic acid Schiff‘s (PAS), Alcian blue methods (pH-2.5) and Gomori’s cobalt method for Alkaline phosphatase and Acid phosphatase. The Succinic dehydrogenase enzyme was also estimated on cryostat sections.Result: The observations on kidneys of Guinea fowl were accomplished after macroscopic, histomorphological and histochemical means. This reddish-brown colored paired organ of kidneys was retroperitoneally placed in synsacral fossa and had incomplete division of three parts. The wrapping material of capsule was the dense connective tissue. The outer cortex and inner medulla had different parts of nephrons. Two types of nephrons viz. mammalian type (with Henle loop) and reptilian type (without Henle loop) were observed. Collagen, elastic and reticular fibers were there in different components of the kidney. Histochemically it has determined the secretion of neutral as well as acidic mucopolysaccharide substances. PAS activity was observed at mesangial cells and basement membrane of DCT. The Alkaline phosphatase showed the positive activity in capsule, brush border of proximal and distal convoluted tubules. The Acid phosphatases were showing intense activity in parietal and visceral layer of renal corpuscles, lumen and basement membrane of PCT. The intense activity of Succinic dehydrogenase showed at PCT, DCT, collecting tubule whereas renal corpuscles showed feeble activity.

Enzymhistochemische Untersuchungen der Augentumoren von Rindern. I. Enzymhistochemische Befunde an Papillomen und Fibromen

1973 ◽  
Vol 10 (3) ◽  
pp. 257-265
Author(s):  
I. Ivascu ◽  
E. Muresan ◽  
Z. Papay
Keyword(s):  
Alkaline Phosphatase ◽  
Adenosine Triphosphatase ◽  
Succinic Dehydrogenase ◽  
Cell Multiplication ◽  
Succinic Dehydrogenase Activity ◽  
Normal Tissues ◽  
Multiplication Process ◽  
Topographical Distribution ◽  
Orbital Region ◽  
Nonspecific Esterases

The activity of succinic dehydrogenase, adenosine triphosphatase, acid and alkaline phosphatase, lipase and nonspecific esterases in 12 tumors of the orbital region of cattle was studied. The succinic dehydrogenase activity was not significantly different in papillomas and fibromas, as compared with that of the normal tissues of origin of the tumors. Topographical distribution of adenosine triphosphatase was directly correlated with the cell multiplication process, and thus with the tissues' needs for an energetic basis. The activity of adenosine triphosphatase and alkaline phosphatase was enhanced in the region of proliferating capillaries, and this was interpreted to indicate the tendency of these tumors to malignancy. The activity of lipase and the nonspecific esterases showed nonspecific oscillations in papillomas and fibromas as compared with that in the normal tissues.

Histology and histochemistry of elongating needles of Pinus strobus subjected to a long-duration, low-concentration exposure of sulfur dioxide

1981 ◽  
Vol 59 (12) ◽  
pp. 2558-2567 ◽  
Author(s):  
K. E. Percy ◽  
R. T. Riding
Keyword(s):  
Growth Parameters ◽  
Succinic Dehydrogenase ◽  
Pinus Strobus ◽  
Wall Region ◽  
Growth Reduction ◽  
Succinic Dehydrogenase Activity ◽  
Long Duration ◽  
Bud Set ◽  
Total Carbohydrates ◽  
External Growth

Two-year-old seedlings of Pinus strobus were grown from budbreak to bud set (11 weeks) in air containing 132 ± 26 μg SO2∙m−3. Histological and histochemical effects on elongating needles were examined. Cellular injury was restricted to mesophyll parenchyma. Affected cells manifested a progressive alteration of protoplast staining proportional to the degree of injury. Total carbohydrates and plastids aggregated at cell walls. Total proteins and proteins containing sulfhydryl–disulfide groups decreased. Phospholipid staining in the plasmalemma – cell wall region appeared reduced. Succinic dehydrogenase activity was enhanced and was apparent longer in injured cells. Needle ontogeny was slowed in fumigated seedlings. There were no significant differences in external growth parameters after 11 weeks. The injury can, therefore, be classified as latent or hidden. The SO2 effects could contribute to a growth reduction in successive increments.

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