Morphological features of spermatozoa of Paragonimus ohirai (Trematoda: Platyhelminthes) examined by a silver nitrate staining technique

Parasitology ◽  
1991 ◽  
Vol 103 (1) ◽  
pp. 103-110 ◽  
Author(s):  
H. Hirai ◽  
I. Tada
Keyword(s):  
Silver Nitrate ◽  
Spiral Structure ◽  
Staining Technique ◽  
Sperm Nucleus ◽  
Morphological Features ◽  
Silver Nitrate Staining ◽  
Different Characteristics

The mature thread-like spermatozoon of Paragonimus ohirai was shown to be composed of 7 distinct segments which have morphologically different characteristics, following staining with silver nitrate. The first segment (1S) is a needle-shaped structure with a perforatorium. The 2S is composed of two filaments loosely paired; 3S consists of a somewhat coarse thread (vestige of the cytoplasmic projection); 4S is composed of two flagella tightly paired; in 5S, two silver-positive filaments are independently seen as in 2S; 6S has a ‘spiral’ structure composed of two flagella; in 7S, two filaments are seen dissociated from each other. The sperm nucleus exists within the ‘spiral’ structure of 6S and this structure may serve to hold and/or protect the nucleus. The technique presented here is useful for gross morphological observations of the long sperm of flatworms.

Study of DNA integrity in somatic cells and spermatozoa by single-cell gel electrophoresis with silver-nitrate staining

2008 ◽  
Vol 2 (1) ◽  
pp. 87-92
Author(s):  
S. N. Proshin ◽  
G. V. Stepanov ◽  
V. Yu. Kravtsov ◽  
G. P. Kosyakova ◽  
I. A. Paranyan ◽  
...  
Keyword(s):  
Silver Nitrate ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Somatic Cells ◽  
Dna Integrity ◽  
Single Cell Gel Electrophoresis ◽  
Silver Nitrate Staining

Staining SDS-PAGE Gels of Skeletal Matrices after Western Blot: A Way to Improve their Sharpness

2016 ◽  
Vol 672 ◽  
pp. 215-221 ◽  
Author(s):  
Frédéric Marin ◽  
Françoise Immel ◽  
Nolwenn Trinkler ◽  
Danièle Gaspard
Keyword(s):  
Western Blot ◽  
Silver Nitrate ◽  
Sds Page ◽  
Simple Protocol ◽  
Classical Technique ◽  
The Matrix ◽  
Partial Transfer ◽  
Silver Nitrate Staining ◽  
Extra Step ◽  
Calcified Tissues

Denaturing 1D electrophoresis on acrylamide gels, also referred as SDS-PAGE, is a classical technique for fractionating and visualizing the macromolecular constituents of matrices associated to calcified tissues. This technique has been widely used in association with the subsequent silver nitrate staining. But because matrices associated to calcified tissues are very often glycosylated and constituted of numerous polydisperse macromolecules, the obtained pattern is frequently ‘smeary’ and discrete bands, when present on the gel, are often blurred and thickened. In this paper, we present a simple protocol that can circumvent this drawback and ‘clean’ the gels. In short, after the classical migration step of the matrix macromolecules, the gel is electro-blotted on a PVDF membrane, similarly to a Western blot, but for a shorter time (partial transfer, i.e., one hour or less). It is subsequently stained with silver nitrate. The likely effect of the transfer is to partly remove polydisperse macromolecules and to ‘sharpen’ the discrete bands. We think that this extra-step may improve in several cases the gel pictures, particularly when they are blurred. We illustrate this phenomenon with two examples taken from brachiopod and mollusc shell matrices.

scholarly journals THE INDUCTION OF MELANIZATION IN GOLDFISH SCALES WITH ACTH, IN VITRO

1963 ◽  
Vol 18 (1) ◽  
pp. 181-194 ◽  
Author(s):  
Alden V. Loud ◽  
Yutaka Mishima
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Silver Nitrate ◽  
Light And Electron Microscopy ◽  
Subcellular Structure ◽  
Acth Stimulation ◽  
Cytoplasmic Bodies ◽  
Silver Nitrate Staining ◽  
Stimulation Of

The induction of melanization in xanthic goldfish scales with ACTH in vitro has been studied by light and electron microscopy utilizing ammoniated silver nitrate staining of premelanin and melanin. The melanized cells (melanophores and melanocytes) and the yellow pigmented cells (lipophores and the newly described lipocytes) were found to possess many similarities at the levels of cellular and subcellular structure. The latter cells contain characteristic cytoplasmic bodies which react positively to the premelanin stain. Changes accompanying ACTH stimulation of goldfish scales in tissue culture suggest that these bodies in the lipocytes and lipophores can become melanized. Electron micrographs illustrate the intermediate staining of newly formed melanin granules in an induced melanocyte and the appearance of a transitional melanolipophore. It is postulated that ACTH can promote the association of the enzyme tyrosinase with the preformed structure of unmelanized granules.

scholarly journals Shells, Ripples and Tails

1999 ◽  
Vol 186 ◽  
pp. 165-172
Author(s):  
D. Carter
Keyword(s):  
Spiral Structure ◽  
Morphological Features ◽  
Disk Galaxies ◽  
Tidal Tails

It has been known since the early simulations of Wright (1972), and Toomre & Toomre (1972), that interactions between galaxies can give rise to quite spectacular morphological features, including spiral structure in disk galaxies, and extensive tails. It appears that long tidal tails only arise from interactions involving disk galaxies (Toomre & Toomre 1972), and thus the presence of two opposed long tidal tails in a number of disturbed galaxies such as the Antennae (Whitmore & Schweizer 1995), NGC 3921 (Schweizer 1996), and NGC 7252 (Schweizer 1982), has led to the interpretation of these galaxies as disk-disk mergers in progress. These systems can be modeled rather successfully, as the work of Hibbard & Mihos (1995) has shown.

A comparison of sperm morphology and silver nitrate staining characteristics in the domestic ferret and the black-footed ferret

1989 ◽  
Vol 22 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Patrick T. Curry ◽  
Terry Ziemer ◽  
Gerhard Van der Horst ◽  
Warren Burgess ◽  
Monte Straley ◽  
...  
Keyword(s):  
Silver Nitrate ◽  
Sperm Morphology ◽  
Silver Nitrate Staining

scholarly journals Cytogenetic Studies on Three Species of Genus Burmagomphus of Family Gomphidae (Odonata: Anisoptera) from India

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Walia Gurinder Kaur ◽  
Chahal Sarabjit Singh ◽  
Singh Navdeep
Keyword(s):  
Chromosome Number ◽  
Germ Cell ◽  
Silver Nitrate ◽  
X Chromosome ◽  
Type Number ◽  
Autosomal Bivalents ◽  
Specific Staining ◽  
The Family ◽  
Cytogenetic Studies ◽  
Silver Nitrate Staining

Male germ cell chromosomes of Burmagomphus divaricatus, Burmagomphus pyramidalis and Burmagomphus sivalikensis of family Gomphidae have been investigated by using conventional staining, C-banding, silver nitrate staining and sequence specific staining. The species were collected from Punjab and Himachal Pradesh, India. All the species possess the chromosome number 2n = 23 which is the type number of the family. Terminal C bands and NOR’s are present at the autosomal bivalents and X chromosome is C positive and NOR rich in all the three species, while m bivalents show variation in distribution of C- heterochromatin and NOR’s. In the sequence specific staining, whole complement shows bright DAPI signals in B. divaricatus, bright CMA3 signals in B. pyramidalis and both DAPI and CMA3 signals in B. sivalikensis.

scholarly journals Cytogenetic characterization of Hypostomus nigromaculatus (Siluriformes: Loricariidae)

2008 ◽  
Vol 6 (1) ◽  
pp. 93-100 ◽  
Author(s):  
Marceléia Rubert ◽  
Cláudio H. Zawadzki ◽  
Lucia Giuliano-Caetano
Keyword(s):  
Silver Nitrate ◽  
Current Knowledge ◽  
Color Variation ◽  
Giemsa Staining ◽  
Dapi Staining ◽  
The Family ◽  
Silver Nitrate Staining ◽  
Identification Of Species ◽  
Cytogenetic Characterization

Hypostomus is the most speciose genus in the family Loricariidae, with approximately 120 species. These fish show a wide morphological and color variation, which hinders the identification of species, mainly of widely distributed representatives. The aim of this study was to contribute to the current knowledge on cytogenetic features of Hypostomus nigromaculatus. Three specimens of H. nigromaculatus, collected in two tributaries of rio Tibagi, Paraná, and in Cachoeira de Emas, rio Mogi-Guaçu, São Paulo, the latter being the type locality of H. nigromaculatus, were studied. Chromosomal preparations were submitted to Giemsa staining, silver nitrate impregnation, C-banding and CMA3 and DAPI fluorochromes staining. All samples presented 2n = 76, but the rio Mogi-Guaçu sample differed from those from tributaries of rio Tibagi in relation to karyotype formulae, distribution and composition of heterochromatin, and NOR location. The silver nitrate staining revealed the presence of multiple Ag-NORs for all samples, but with differences on the location on chromosomes. CMA3 staining reveled bright signals equivalent to NOR-bearing chromosomal segments; such sites were characterized by negative, i.e. unstained, marks after DAPI staining. The pattern of heterochromatin distribution was distinctive among samples from rio Mogi-Guaçu and tributaries of rio Tibagi. The differences observed between the sample from rio Mogi-Guaçu and the ones from tributaries of rio Tibagi allow us to suggest that these samples are presently isolated. Further analyses are necessary to ascertain whether such isolation refers to distinct populations or characterizes true different species.

Silver staining of the cortical reaction in oocytes of Marthasterias glacialis (Echinodermata, Asteroidea)

1987 ◽  
Vol 65 (11) ◽  
pp. 2607-2611 ◽  
Author(s):  
Mário Sousa ◽  
Carlos Azevedo
Keyword(s):  
Silver Nitrate ◽  
Lamellar Structure ◽  
The Other ◽  
Dense Matrix ◽  
Perivitelline Space ◽  
Cortical Granules ◽  
Silver Deposits ◽  
The Matrix ◽  
Silver Nitrate Staining ◽  
Marthasterias Glacialis

Morphologically, cortical granules of the starfish Marthasterias glacialis present a lamellar structure within a dense matrix. However, with silver nitrate staining, the matrix is subdivided into argyrophilic and argyrophobic materials. Just before exocytosis the cortical granules swell. After being released in the perivitelline space, the unstained matrix disperses while the other components are incorporated into the vitelline layer. When cells are incubated with pronase before exposure to silver nitrate, no silver deposits are found.

Sign in / Sign up

Export Citation Format

Share Document