Trichinella strain, pig race and other parasitic infections as factors in the reliability of ELISA for the detection of swine trichinellosis

Parasitology ◽  
1992 ◽  
Vol 105 (1) ◽  
pp. 111-115 ◽  
Author(s):  
F. Serrano ◽  
E. Pérez ◽  
D. Reina ◽  
I. Navarrete
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
Post Infection ◽  
False Positive ◽  
Maximum Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Low Grade ◽  
High Background ◽  
Cross Reactions

An enzyme-linked immunosorbent assay (ELISA) using crude worm extracts (CWE) and mixtures of these as antigens of five Spanish isolates (P, C, B1, B2 and W) was developed for detecting homologous and heterologous experimental infections with these isolates between – 14 and 82 days post-infection (p.i.) in white and Iberian pigs. A total of 243 pigs (Ilberian or cross-bred with this race) with numerous parasitic infections were also screened for the presence of antibodies to a mixture of CWE of C, B1 and B2 isolate. The test showed a specificity of 93·1–98·9% depending on the cut-off values and a maximum sensitivity of 92·8–100% between days 34 and 82 p.i. A low grade of infectivity was shown in the T3 isolates compared to the T1 isolates (P, C, B1 and B2) but high cross-reactions were observed between all the isolates with minor differences between P and W isolates. The highest antibody response was found in P infections and the lowest in pigs infected with the W isolate. A clear association between the presence of several parasitic infections and false positive reactions was not found, but an important relation was shown between high background levels and the Iberian race in experimentally and conventionally raised pigs

scholarly journals Detection of False-Positive Sera in Contagious Agalactia with a Multiantigen ELISA and their Elimination with a Protein G Conjugate

1998 ◽  
Vol 10 (4) ◽  
pp. 326-330 ◽  
Author(s):  
Maurice Lambert ◽  
Michel Calamel ◽  
Philippe Dufour ◽  
Evelyne Cabasse ◽  
Christian Vitu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein G ◽  
Mycoplasma Agalactiae ◽  
Cross Reactions ◽  
Serologic Diagnosis ◽  
Contagious Agalactia ◽  
The Past

In serology, lack of specificity can generally be attributed to cross-reactions between different pathogens with antigens bearing similar epitopes. During seroepidemiologic surveys of contagious agalactia of sheep caused by Mycoplasma agalactiae infection, numerous sera were analyzed by enzyme-linked immunosorbent assay (ELISA). A few sera reacted with various antigens coated on plates, including the well with no antigen. This reactivity was not due to cross-reactions as initially suspected, and these multipositive sera were designated false-positive sera. Elimination of this false positivity was not possible by using covalent ELISA plates or different rabbit anti-sheep IgG conjugates. Only conjugates using monoclonal antibodies or protein G were efficient in elimination of false positivities without reducing the true specific positive titers. No false-positive sera have been observed since the implementation of protein G conjugates in the serologic diagnosis of contagious agalactia by ELISA for the past 2 years.

scholarly journals Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Results

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

scholarly journals Analysis of Specific Immunoglobulin G Subclass Antibodies for Serological Diagnosis of Echinococcosis by a Standard Enzyme-Linked Immunosorbent Assay

1998 ◽  
Vol 5 (5) ◽  
pp. 613-616 ◽  
Author(s):  
Felix Grimm ◽  
Friedrich E. Maly ◽  
Jian Lü ◽  
Roberto Llano
Keyword(s):  
Healthy Volunteers ◽  
Immunosorbent Assay ◽  
Immunoglobulin G ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Igg Subclass ◽  
Specific Antibodies ◽  
Specific Igg4 ◽  
Antibody Levels

ABSTRACT The potential roles of specific antibodies of the different immunoglobulin G (IgG) subclasses in the serological diagnosis of cystic echinococcosis (CE) and alveolar echinococcosis (AE) were investigated by an enzyme-linked immunosorbent assay based on hydatid fluid as antigen. Specific antibodies of subclass 1 were found to be of major importance. In sera collected at the time of diagnosis (i.e., before any therapeutic intervention was initiated) they could be demonstrated in 14 of 15 sera from patients with CE and in all 12 sera from patients with AE. The most discriminatory and the most specific antibodies found in this study belonged to IgG subclass 4. Only one false-positive reaction was observed with 253 sera from healthy volunteers, and no cross-reactions occurred in 80 sera from patients with different parasitic infections. Specific IgG4 antibodies could be demonstrated in 61.0 to 66.7% (CE) or 47.6 to 66.7% (AE) of the cases. Antibody levels of IgG subclass 2 were elevated only moderately, and subclass 3 antibodies were detected in a few cases only. In addition, nonspecific reactions in sera of healthy volunteers or patients with other parasitic infections could partially be attributed to antibodies of subclasses 2 and 3.

Effect of Early Immunization on Antibody Response to Reimmunization with Measles Vaccine as Demonstrated by Enzyme-Linked Immunosorbent Assay (ELISA)

PEDIATRICS ◽  
1984 ◽  
Vol 74 (1) ◽  
pp. 90-93
Author(s):  
M. Dianne Murphy ◽  
Philip A. Brunell ◽  
Alan W. Lievens ◽  
Ziad M. Shehab
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
San Antonio ◽  
Significant Risk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Measles Vaccine ◽  
Young Infants ◽  
Antibody Titers

A measles epidemic in San Antonio, Texas provided a population of children who were immunized at ≤10 months of age and reimmunized at ≥15 months of age. Of these children, 302 were evaluated for measles antibody by the sensitive enzyme-linked immunosorbent assay (ELISA), and their responses were compared with those of 300 children who had been immunized at the customary time (≥15 months) with a single immunization. There were only five seronegative findings in each group. The children immunized at the customary time did have significantly higher (P < .001) antibody titers than the children immunized at ≤10 months and reimmunized at ≥15 months. These results indicate that early immunization followed by reimmunization may be indicated when young infants are at significant risk of measles exposure. This approach should not create an increased number of serologically nonresponsive children when reimmunized at ≥15 months.

scholarly journals Assessment of Mumps Virus-Specific Antibodies: Comparison of Plaque Reduction Neutralization Test and Enzyme-Linked Immunosorbent Assay Estimates

2019 ◽  
Vol 220 (9) ◽  
pp. 1462-1468 ◽  
Author(s):  
Stéphanie Ravault ◽  
Damien Friel ◽  
Emmanuel Di Paolo ◽  
Adrian Caplanusi ◽  
Paul Gillard ◽  
...  
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
Neutralizing Antibodies ◽  
Neutralization Test ◽  
Pearson Correlation ◽  
Mumps Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Moderate Correlation ◽  
Plaque Reduction Neutralization Test

Abstract Background The plaque reduction neutralization test (PRNT), which measures a subset of immunoglobulin antibodies (functional neutralizing antibodies), and the enzyme-linked immunosorbent assay (ELISA), which measures total immunoglobulin (neutralizing and nonneutralizing antibodies), characterize different aspects of the anti–mumps virus antibody response after vaccination. Methods Data from a recent phase 3 clinical trial (NCT01681992) of 2 measles-mumps-rubella vaccines were used to compare anti-mumps antibody responses measured using an unenhanced PRNT (GSK; seropositivity cutoff and threshold, 2.5 and 4 times the 50% end-point dilution, respectively) with those estimated using an ELISA (thresholds, 5 and 10 ELISA units/mL, respectively). Results Of 3990 initially seronegative samples, 3284 (82.3%) were seropositive after vaccination for anti-mumps antibodies in both assays. The Pearson correlation coefficient for double-positive samples was 0.57, indicative of a moderate correlation. Receiver operating characteristic curve analysis showed that an ELISA threshold of 51.7 ELISA units/mL best corresponded to the PRNT seroresponse threshold. There was no obvious vaccine brand effect on the correlation between assays. Conclusions The moderate correlation between the anti-mumps antibody measurements obtained with PRNT and ELISA reflects different aspects of the serological response. In the absence of a well-defined protective serological threshold, PRNT provides complementary information on the antibody response, whereas ELISA remains a critically useful measurement of vaccine immunogenicity.

Evaluation of immunoglobulin G4 subclass antibody in a peptide-based enzyme-linked immunosorbent assay for the serodiagnosis of human fascioliasis

Parasitology ◽  
2007 ◽  
Vol 134 (14) ◽  
pp. 2021-2026 ◽  
Author(s):  
C. TANTRAWATPAN ◽  
W. MALEEWONG ◽  
C. WONGKHAM ◽  
S. WONGKHAM ◽  
P. M. INTAPAN ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Large Scale ◽  
Laboratory Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Parasitic Infections ◽  
Immunoglobulin G4 ◽  
Predictive Values ◽  
Diagnostic Potential ◽  
Human Fascioliasis ◽  
Specific Igg4

SUMMARYTo improve the diagnosis of human fascioliasis caused byFasciola gigantica, we developed a peptide-based enzyme-linked immunosorbent assay (peptide-based ELISA) based on the detection of specific IgG4 subclass antibody. Two identified B-cell epitopes ofF. giganticacathepsin L1 were synthesized as single synthetic peptides, acetyl-DKIDWRESGYVTELKDQGNC-carboxamide (peptide L) and acetyl-DKIDWRESGYVTEVKDQGNC-carboxamide (peptide V), and their diagnostic potential was evaluated. The sera of 25 patients infected withF. gigantica, 212 patients with other parasitic infections, 32 cholangiocarcinoma patients and 57 healthy controls were analysed. The sensitivity, specificity, accuracy, and positive and negative predictive values of this assay were the same with both peptides at 100%, 99·7%, 99·7%, 96·2% and 100%, respectively. These highly sensitive and specific peptide-based ELISAs for the detection of specific IgG4 antibody could be useful for laboratory diagnosis of human fascioliasis in future large-scale surveys throughout Southeast Asia where this disease is prevalent.

Antibody response toShigella sonnei infection determined by an enzyme-linked immunosorbent assay

1983 ◽  
Vol 2 (3) ◽  
pp. 200-205 ◽  
Author(s):  
E. Ekwall ◽  
S. Hæggmann ◽  
M. Kalin ◽  
B. Svenungsson ◽  
A. A. Lindberg
Keyword(s):  
Antibody Response ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay
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