The ultrastructural localization of Echinococcus granulosus antigen 5

Parasitology ◽  
1996 ◽  
Vol 113 (3) ◽  
pp. 213-222 ◽  
Author(s):  
M. K. Jones ◽  
L.-H. Zhang ◽  
G. R. Leggatt ◽  
D. J. Stenzel ◽  
D. P. McManus
Keyword(s):  
Echinococcus Granulosus ◽  
Ultrastructural Localization ◽  
Extracellular Matrices ◽  
Hydatid Cysts ◽  
Polyclonal Antisera ◽  
Parenchymal Cells ◽  
External Face ◽  
Basal Matrix ◽  
Antigen 5 ◽  
Cytoplasmic Labelling

SUMMARYMurine monoclonal and polyclonal antisera, raised against the 38 kDa subunit of Echinococcus granulosus antigen 5, were used to investigate the tissue distribution of the antigen in hydatid cysts. Immunoreactivity was visualized by indirect immunofluorescence on whole protoscoleces, and ultrastructural immunocytochemistry utilizing colloidal gold-based labelling procedures on unsectioned and cryosectioned brood capsules and protoscoleces. In protoscoleces, the 38 kDa subunit of antigen 5 was localized at the interface of parenchymal cells and associated extracellular matrices, as well as along the interface of the tegumentary syncytium in the somal region and its basal matrix. Cytoplasmic labelling of parenchymal cells was rare; when observed, it was associated with vesicles and membranes in cytoplasmic extensions of parenchymal cells. In brood capsules, the antigen was associated with the external face of the plasma of degenerating parenchymal cells. The 38 kDa subunit occurs along the extracellular face of cell membranes, suggesting that antigen 5 is either a component of the membranes or associated extracellular matrices.

Ultrastructural immunocytochemical localization of two hydatid fluid antigens (antigen 5 and antigen B) in the brood capsules and protoscoleces of ovine and equine Echinococcus granulosus and E. multilocularis

Parasitology ◽  
1978 ◽  
Vol 77 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Caroline Davies ◽  
M. D. Rickard ◽  
D. T. Bout ◽  
J. P. Smyth
Keyword(s):  
Echinococcus Granulosus ◽  
Periodic Acid ◽  
Ultrastructural Localization ◽  
Capsule Wall ◽  
Periodic Acid Schiff ◽  
Hydatid Fluid ◽  
Antigen B ◽  
Parenchyma Cells ◽  
Perinuclear Cytoplasm ◽  
Antigen 5

SummaryThe unlabelled antibody method was used in the ultrastructural localization of two hydatid fluid antigens, antigen 5 and antigen B, in brood capsules and protoscoleces of Echinococcus granulosus and E. multilocularis. Antigen 5 was found in the parenchyma cells of the protoscolex and brood capsule wall and to a lesser extent in the walls of the flame cells and collecting ducts of the excretory system and in the surrounding interstitial material. It is suggested that, while some excretion of this antigen may occur from the protoscolex, it could also be liberated into the cystic cavity by degeneration of protoscoleces and parenchymal cells of the brood capsule wall. Antigen B was found mainly in the distal cytoplasm and perinuclear cytoplasm of the tegument anterior to the suckers. It is apparently secreted to the outside and was present in the brood capsule contents; it adheres to the anterior surface and the posterior periodic acid–Schiff (PAS)-positive glycocalyx of the protoscolex and to the inner surface of the brood capsule wall. The protoscolex tegument posterior to the suckers was negative. The parenchyma cells of the protoscolex and brood capsule wall were also positive although the intensity of the reaction product was variable.

Ultrastructural localization of an Echinococcus granulosus laminin-binding protein

Parasitology ◽  
1999 ◽  
Vol 118 (3) ◽  
pp. 319-325
Author(s):  
M. K. JONES ◽  
L.-H. ZHANG ◽  
R. J. GOULD ◽  
D. P. McMANUS
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Echinococcus Granulosus ◽  
Binding Protein ◽  
Ultrastructural Localization ◽  
Extracellular Matrices ◽  
Native Protein ◽  
Low Degree ◽  
Laminin Binding Protein ◽  
Laminin Binding

Murine antibodies, raised against a purified recombinant 30 kDa laminin-binding protein from Echinococcus granulosus, were used to investigate the tissue distribution of the native protein in protoscoleces and brood capsules. Immunofluorescence, in combination with confocal microscopy, revealed that the protein was distributed in small annular foci near the peripheral regions of the protoscoleces. Immunoelectron microscopy of thawed cryosections demonstrated that the laminin-binding protein was present in the cytoplasm of tegumentary cytons and myocytons, but not in cells of the excretory system. The protein was associated with amorphous regions of the cytoplasm, and was not expressed at the surfaces of cells. This distribution resembles those of other invertebrate laminin-binding proteins, which are thought to act in the cell cycle and cell proliferation events. A low degree of label was consistently detected in extracellular matrices of the protoscolex.

scholarly journals Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 125
Author(s):  
Piero Bonelli ◽  
Silvia Dei Giudici ◽  
Angela Peruzzu ◽  
Lorena Mura ◽  
Cinzia Santucciu ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Animal Species ◽  
Cost Effective ◽  
Sensu Stricto ◽  
Sybr Green ◽  
Hydatid Cysts ◽  
Genotype Identification ◽  
Host Selectivity ◽  
Nad5 Gene ◽  
Echinococcus Granulosus Sensu Lato

Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis in animals and humans. Different E. granulosuss.l. genotypes exhibit great diversity in their life cycle, host selectivity and pathogenicity. For this reason, the study of genetic variation within Echinococcus species is of importance for their epidemiological implication. We employed two SNP genotyping technologies to distinguish G1 and G3 E. granulosus sensu stricto (s.s.). genotypes. The genotypes of DNA samples (n = 28) extracted from hydatid cysts of different animal species were identified by amplification and sequencing of a fragment of the mitochondrial nad5 gene. Two SYBR green and three TaqMan real time PCR assays were developed for targeting of three nad5 informative positions (SNP758, 1123, and 1380) known to be able to discriminate G1 from G3. Genotyping by SYBR Green PCR based on cycle threshold (Ct) with melting temperature (Tm) analysis and performed on SNP1123 and SNP1380 failed to identify one DNA sample. TaqMan assays for SNP758, 1123 and 1380 effectively confirmed genotype identification obtained by Sanger sequencing. Our results demonstrated that the combination of the three Taqman assays developed in this study represents a valuable and cost effective tool alternative to DNA sequencing for E. granulosus s.s. genotyping.

Observations on the suitability and importance of the domestic intermediate hosts of Echinococcus granulosus in Uttah Pradesh, India

1989 ◽  
Vol 63 (1) ◽  
pp. 39-45 ◽  
Author(s):  
M. Irshadullah ◽  
W. A. Nizami ◽  
C. N. L. Macpherson
Keyword(s):  
Life Cycle ◽  
Intermediate Host ◽  
Echinococcus Granulosus ◽  
Frequency Distribution ◽  
Host Species ◽  
Control Measures ◽  
The Other ◽  
Hydatid Cysts ◽  
Intermediate Hosts ◽  
Cyst Size

ABSTRACTThe present study investigated the suitability and importance of buffaloes, camels, sheep, goats and pigs in maintaining the life-cycle of Echinococcus granulosus in Aligarh, India. A total of 565 (36%) of 1556 buffaloes, 20 (2%) of 1208 goats, 5 (1%) of 559 pigs, 6 (6%) of 109 sheep and two of three camels were found to harbour hydatid cysts. The frequency distribution of the hydatid cysts in each intermediate host species was over-dispersed and in buffaloes cyst fertility increased with increasing cyst size. Of 2171, 95 and four buffalo, goat, and camel cysts examined 327 (15%), two (2%) and three cysts respectively were fertile. No pig or sheep cysts were found to contain protoscoleces. The unfenced buffalo abattoir and the large number of dogs allowed access to the abattoir coupled to the number of buffaloes slaughtered in comparison to the other potential hosts, indicates that the buffalo is the most significant host for maintaining the life-cycle of the parasite in this area of India. Applicable control measures for the region are suggested.

Metacestodes of moose, Alces alces, of the Chapleau Crown Game Preserve, Ontario

1979 ◽  
Vol 57 (8) ◽  
pp. 1619-1623 ◽  
Author(s):  
E. M. Addison ◽  
A. Fyvie ◽  
F. J. Johnson
Keyword(s):  
Echinococcus Granulosus ◽  
Lung Tissue ◽  
Physical Condition ◽  
Alces Alces ◽  
Age Groups ◽  
Hydatid Cysts ◽  
Intensity Of Infection ◽  
Taenia Hydatigena ◽  
Apparent Relationship

Lungs, liver, spleen, heart, and kidneys of moose, Alces alces, collected from 1963 to 1965 in the Chapleau Crown Game Preserve of northeastern Ontario were examined for parasites. Thirty-eight of 51 moose (75%) were infected with metacestodes of Taenia hydatigena Pallas, 1766; 40 of 54 (74%) with Taenia krabbei Moniez, 1879; and 36 of 54 (67%) with Echinococcus granulosus (Batsch, 1786). Twenty-two of 51 (43%) moose harboured all three species. Each species was more prevalent in older moose than in young moose and intensity of infections increased with the age of moose. Occurrence of small hydatid cysts decreased and large cysts increased with increasing age of moose. Of 1154 hydatid cysts, 95.3% were in lung tissue, 3.6% in liver, 0.9% in spleen, and 1 cyst (0.1%) was recovered from each of heart and kidney. Degenerate cysticerci of T. hydatigena and T. krabbei were observed in all age groups of moose. There was no apparent relationship between intensity of infection with metacestodes and physical condition of moose.

Genetic characterization of human hydatid cysts shows coinfection by Echinococcus canadensis G7 and Echinococcus granulosus sensu stricto G1 in Argentina

2017 ◽  
Vol 116 (9) ◽  
pp. 2599-2604 ◽  
Author(s):  
María Florencia Debiaggi ◽  
Silvia Viviana Soriano ◽  
Nora Beatriz Pierangeli ◽  
Lorena Evelina Lazzarini ◽  
Luis Alfredo Pianciola ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Genetic Characterization ◽  
Sensu Stricto ◽  
Hydatid Cysts ◽  
Echinococcus Canadensis ◽  
Echinococcus Granulosus Sensu Stricto
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