scholarly journals Antagonistic activity of the fungusPochonia chlamydosporiaon mature and immatureToxocara caniseggs

Parasitology ◽  
2012 ◽  
Vol 139 (8) ◽  
pp. 1074-1085 ◽  
Author(s):  
A. S. MACIEL ◽  
L. G. FREITAS ◽  
L. D. FIGUEIREDO ◽  
A. K. CAMPOS ◽  
I. N. K. MELLO
Keyword(s):  
Direct Relationship ◽  
Toxocara Canis ◽  
Nematophagous Fungus ◽  
Antagonistic Activity ◽  
Dialysis Membrane ◽  
Inoculum Level ◽  
Pochonia Chlamydosporia ◽  
Infective Larvae ◽  
Biological Capacity

SUMMARYIn vitrotests were performed to evaluate the ability of 6 isolates of the nematophagous fungusPochonia chlamydosporiato infect immature and matureToxocara caniseggs on cellulose dialysis membrane. There was a direct relationship between the number of eggs colonized and the increase in the days of interaction, as well as between the number of eggs colonized and the increase in the concentration of chlamydospores (P<0·05). Immature eggs were more susceptible to infection than mature eggs. The isolate Pc-04 was the most efficient egg parasite until the 7th day, and showed no difference in capacity to infect mature and immature eggs in comparison to Pc-07 at 14 and 21 days of interaction, respectively. Isolate Pc-04 was the most infective on the two evolutionary phases of the eggs at most concentrations, but its ability to infect immature eggs did not differ from that presented by the isolates Pc-07 and Pc-10 at the inoculum level of 5000 chlamydospores. Colonization of infective larvae inside or outside the egg was observed in treatments with the isolates Pc-03, Pc-04, Pc-07 and Pc-10. The isolate Pc-04 ofP. chlamydosporiahas great biological capacity to destroy immature and matureT. caniseggs in laboratory conditions.

scholarly journals Meloidogyne javanica control by Pochonia chlamydosporia, Gracilibacillus dipsosauri and soil conditioner in tomato

2013 ◽  
Vol 39 (2) ◽  
pp. 122-125 ◽  
Author(s):  
Guilherme Silva de Podestá ◽  
Leandro Grassi de Freitas ◽  
Rosangela Dallemole-Giaretta ◽  
Ronaldo João Falcão Zooca ◽  
Larissa de Brito Caixeta ◽  
...  
Keyword(s):  
Meloidogyne Javanica ◽  
Positive Control ◽  
Nematophagous Fungus ◽  
Antagonistic Activity ◽  
Negative Control ◽  
Control Treatment ◽  
In Vitro Tests ◽  
Pochonia Chlamydosporia ◽  
Soil Conditioner

Organic matter plays a fundamental role in the antagonistic activity of microorganisms against phytonematode populations on the soil. In this study, the compatibility between the fungus Pochonia chlamydosporia (Pc-12) and the rhizobacterium Gracilibacillus dipsosauri (MIC 14) was evaluated in vitro, as well as the effect of the fungus at the concentration of 5,000 chlamydospores per gram of soil, rhizobacterium at 4.65 x 10(9) cells/g of soil, and the soil conditioner Ribumin® at 10 g/pot, either alone or in combination, against Meloidogyne javanica population in tomato plants (3,000 eggs/pot). A suspension of water or Ribumin® alone was applied on the soil as negative control, while a suspension of nematode eggs was applied as positive control. The reduction in the number of galls in roots per plant was 48 and 41% for the treatments Ribumin + MIC 14 + Pc-12 and MIC 14 + Pc-12, respectively. Regarding to the number of eggs per plant, MIC 14 and Pc-12 + Ribumin led to a reduction by 26 and 21%, respectively, compared to the control treatment. Interaction between the nematophagous fungus and the rhizobacterium was positive for the nematode control, even though G. dipsosauri inhibited P. chlamydosporia growth by up to 30% in in vitro tests.

Identification of a new C-type lectin, TES-70, secreted by infective larvae of Toxocara canis, which binds to host ligands

Parasitology ◽  
2000 ◽  
Vol 121 (5) ◽  
pp. 545-554 ◽  
Author(s):  
A. LOUKAS ◽  
A. DOEDENS ◽  
M. HINTZ ◽  
R. M. MAIZELS
Keyword(s):  
Immune Cell ◽  
Sequence Similarity ◽  
Mannose Receptor ◽  
Toxocara Canis ◽  
Dependent Manner ◽  
Expression Screening ◽  
Lectin Domain ◽  
Calcium Dependent ◽  
Infective Larvae

Infective larvae of the dog roundworm Toxocara canis survive in the tissues of their hosts for extended periods in a state of developmental arrest, successfully evading immune destruction. This survival strategy is thought to be mediated by T. canis excretory/secretory (TES) products which downregulate or divert the immune response. We purified one of the major TES products, TES-70 and gained amino acid sequence from 4 tryptic peptides. These peptides were matched to a predicted protein from a cDNA that was isolated by expression screening a T. canis cDNA library with mouse anti-TES serum. The predicted protein (Tc-CTL-4) is similar to, but larger than, Tc-CTL-1, a 32-kDa C-type lectin secreted by T. canis larvae. Tc-CTL-4 has a signal peptide, 2 Cys-rich domains and a C-terminal calcium-dependent C-type lectin domain that shares sequence similarity with host immune cell receptors such as macrophage mannose receptor and CD23. The lectin domain was expressed in bacteria and antiserum to the purified recombinant protein was used to confirm that Tc-ctl-4 did encode the native TES-70 glycoprotein. TES-70 selectively bound to ligands on the surface of Madin–Darby Canine Kidney cells in vitro in a calcium-dependent manner, inhibitable by mammalian serum, indicating that a host glycan is the native ligand for this new parasite lectin.

scholarly journals Isolation, Selection, and Efficacy of Pochonia chlamydosporia for Control of Rotylenchulus reniformis on Cotton

2005 ◽  
Vol 95 (8) ◽  
pp. 890-893 ◽  
Author(s):  
Kening Wang ◽  
Robert D. Riggs ◽  
Devany Crippen
Keyword(s):  
The United States ◽  
Nematophagous Fungus ◽  
Rice Grain ◽  
In Vitro Test ◽  
Jefferson County ◽  
Rotylenchulus Reniformis ◽  
Cyst Nematodes ◽  
Pochonia Chlamydosporia ◽  
Cotton Fields

The reniform nematode, Rotylenchulus reniformis, is a serious threat to cotton (Gossypium hirsutum) production in the United States, causing an annual loss of about $80 million. The objective of this study was to isolate fungi from eggs of R. reniformis and select potential biocontrol agents for R. reniformis on cotton. We focused on the fungus Pochonia chlamydosporia because it suppresses root-knot and cyst nematodes and because preliminary data indicated that it was present in Arkansas cotton fields. Soil samples were collected from six cotton fields in Jefferson County, Arkansas. A total of 117 isolates of the nematophagous fungus P. chlamydosporia were obtained. In an in vitro test, 105 of the 117 isolates parasitized fewer than 15% of R. reniformis eggs, but 12 isolates parasitized between 16 and 35% of the eggs. These 12 isolates produced from 6.8 × 104 to 6.9 × 105 chlamydospores per gram of medium in vitro, and chlamydospore production was similar on rice grain and corn grain media. In two greenhouse experiments, a single application of isolate 37 (5,000 chlamydospores per gram of soil) significantly reduced the numbers of R. reniformis on cotton roots and in soil. The three isolates (37, 26, and 14) that parasitized the most eggs in vitro were also the most effective in suppressing numbers of R. reniformis and in increasing cotton growth in the greenhouse.

scholarly journals Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

2020 ◽  
Author(s):  
Sebastiao Rodrigo Ferreira ◽  
Alan Rodrigues T. Machado ◽  
Luís Fernando Furtado ◽  
Jose Hugo de S. Gomes ◽  
Raquel M. de Almeida ◽  
...  
Keyword(s):  
Ethyl Acetate Extract ◽  
Parasite Burden ◽  
Nematophagous Fungus ◽  
Nematicidal Activity ◽  
Production Costs ◽  
Pochonia Chlamydosporia ◽  
C Elegans ◽  
Host Health

Abstract Background: Infection by nematodes is a problem for human health, livestock, and agriculture, as it causes deficits in host health, increases production costs, and incurs less food supply. The control of these parasites is usually done using anthelmintics, which, in most cases, have not been fully effective. Therefore, the search for new molecules with anthelmintic potential is necessary. Methods: In the present study, we isolated and characterized molecules from the nematophagous fungus Pochonia chlamydosporia and tested these compounds on three nematodes: Caenorhabditis elegans, Ancylostoma ceylanicum, and Ascaris suum. Results: The ethyl acetate extract showed nematicidal activity on the nematode model C. elegans. We identified the major substance present in two subfractions of this extract as ketamine. Then, we tested this compound on C. elegans and the parasites A. ceylanicum and A. suum using hamsters and mice as hosts, respectively. We did not find a difference between the animal groups when considering the number of worms recovered from the intestines of animals treated with ketamine (6 mg) and albendazole (P > 0.05). The parasite burden of larvae recovered from the lungs of mice treated with ketamine was similar to those treated with ivermectin. Conclusion: These results presented here demonstrated the nematicidal activity of ketamine in vitro and in vivo. Thus, confirming the nematicidal potential of the molecule present in the fungus P. chlamydosporia may consist of a new method of controlling parasites.

Association and predatory capacity of fungi Pochonia chlamydosporia and Arthrobotrys cladodes in the biological control of parasitic helminths of bovines

Parasitology ◽  
2019 ◽  
Vol 146 (10) ◽  
pp. 1347-1351 ◽  
Author(s):  
Ítalo Stoupa Vieira ◽  
Isabela de Castro Oliveira ◽  
Artur Kanadani Campos ◽  
Jackson Victor de Araújo
Keyword(s):  
Biological Control ◽  
Nematophagous Fungus ◽  
Nematophagous Fungi ◽  
Parasitic Nematodes ◽  
Pochonia Chlamydosporia ◽  
Parasitic Helminths ◽  
Infective Larvae ◽  
Volatile Metabolites ◽  
Nematode Parasites ◽  
Predatory Capacity

AbstractNematophagous fungi are used in the biological control of the parasitic helminths of animals and plants. The association of ovicidal and predator nematophagous fungi may present a complementary and increased action on the biological control of helminths. Joint growth compatibility and predation tests were carried out on infective larvae of nematode parasites of bovines with the nematophagous fungus ovicide Pochonia chlamydosporia and the nematophagous fungus predator Arthrobotrys cladodes. The tests of antagonism in direct confrontation, antibiosis and the effect of volatile metabolites between the isolates of P. chlamydosporia and A. cladodes indicated the viability of joint growth of these fungi. The association of the fungi P. chlamydosporia and A. cladodes presented a higher predatory capacity of infective larvae of the parasitic nematodes of bovines when compared to the predation of the fungi used alone. Therefore, under laboratory conditions, the fungi studied presented growth compatibility and the association of these increased the nematicidal activity against parasitic helminths of cattle.

Toxocara canis: Proteolytic enzymes secreted by the infective larvae in vitro

1989 ◽  
Vol 69 (1) ◽  
pp. 30-36 ◽  
Author(s):  
Brian D. Robertson ◽  
A.Ted Bianco ◽  
James H. Mckerrow ◽  
Rick M. Maizels
Keyword(s):  
Proteolytic Enzymes ◽  
Toxocara Canis ◽  
Infective Larvae

scholarly journals Ketamine can be produced by Pochonia chlamydosporia: an old molecule and a new anthelmintic?

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Sebastiao Rodrigo Ferreira ◽  
Alan Rodrigues T. Machado ◽  
Luís Fernando Furtado ◽  
Jose Hugo de S. Gomes ◽  
Raquel M. de Almeida ◽  
...  
Keyword(s):  
Ethyl Acetate Extract ◽  
Parasite Burden ◽  
Nematophagous Fungus ◽  
Nematicidal Activity ◽  
Production Costs ◽  
Pochonia Chlamydosporia ◽  
C Elegans ◽  
Host Health

Abstract Background Infection by nematodes is a problem for human health, livestock, and agriculture, as it causes deficits in host health, increases production costs, and incurs a reduced food supply. The control of these parasites is usually done using anthelmintics, which, in most cases, have not been fully effective. Therefore, the search for new molecules with anthelmintic potential is necessary. Methods In the present study, we isolated and characterized molecules from the nematophagous fungus Pochonia chlamydosporia and tested these compounds on three nematodes: Caenorhabditis elegans; Ancylostoma ceylanicum; and Ascaris suum. Results The ethyl acetate extract showed nematicidal activity on the nematode model C. elegans. We identified the major substance present in two sub-fractions of this extract as ketamine. Then, we tested this compound on C. elegans and the parasites A. ceylanicum and A. suum using hamsters and mice as hosts, respectively. We did not find a difference between the animal groups when considering the number of worms recovered from the intestines of animals treated with ketamine (6 mg) and albendazole (P > 0.05). The parasite burden of larvae recovered from the lungs of mice treated with ketamine was similar to those treated with ivermectin. Conclusions The results presented here demonstrate the nematicidal activity of ketamine in vitro and in vivo, thus confirming the nematicidal potential of the molecule present in the fungus P. chlamydosporia may consist of a new method of controlling parasites.

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