Cellular and humoral immune response of European sea bass (Dicentrarchus labrax L.) (Teleostei: Serranidae) immunized with Sphaerospora dicentrarchi (Myxosporea: Bivalvulida)

Parasitology ◽  
2000 ◽  
Vol 120 (5) ◽  
pp. 465-477 ◽  
Author(s):  
P. MUÑOZ ◽  
A. SITJÀ-BOBADILLA ◽  
P. ÁLVAREZ-PELLITERO
Keyword(s):  
Immune Response ◽  
Stimulation Index ◽  
Dicentrarchus Labrax ◽  
Humoral Response ◽  
Sea Bass ◽  
European Sea Bass ◽  
Immunoblot Assay ◽  
Humoral Immune ◽  
Specific Humoral Response ◽  
Antibody Secreting Cells

The immune response of European sea bass after intracaelomic immunization with Sphaerospora dicentrarchi was studied. Fish were injected with S. dicentrarchi spores (DIC), with spores plus adjuvant (DIC+FCA), with adjuvant alone (FCA) or with PBS. Several parameters of the immune response were measured. Serum lysozyme increased significantly in DIC fish 1 week after immunization (p.i.) and it remained significantly higher in DIC+FCA fish 4 weeks p.i., and in DIC fish 8 weeks p.i. than in PBS-injected fish. The number of nitroblue tetrazolium-positive blood cells was significantly higher in DIC+FCA fish 1, 4 and 8 weeks p.i, but the highest values were detected 1 week p.i. The highest stimulation index was detected in phagocytes from DIC+FCA fish. The number of S. dicentrarchi antibody-secreting cells was significantly higher in DIC+FCA fish than in DIC fish. Serum from DIC and DIC+FCA fish, stained the polar capsules and the valves of S. dicentrarchi spores in immunohistochemistry. Serum antibodies could not be detected using immunoblot assay. All these results show that immunization with S. dicentrarchi resulted in the activation of the non-specific immune response, mainly 7 days p.i. A specific humoral response against the parasite was also demonstrated but it had a low magnitude.

scholarly journals The expression of an intraspecific aggressive reaction in the face of a stressor agent alters the immune response in rats

2005 ◽  
Vol 65 (2) ◽  
pp. 203-209 ◽  
Author(s):  
J. M. Barreto-Medeiros ◽  
E. G. Feitoza ◽  
K. Magalhães ◽  
R. R. da Silva ◽  
F. M. Manhães-de-Castro ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Humoral Response ◽  
Aggressive Response ◽  
Control Group ◽  
Blood Samples ◽  
Humoral Immune ◽  
The Face ◽  
Intraspecific Aggressiveness ◽  
Specific Humoral Response

The repercussion on the immune response of the expression of intraspecific aggressiveness in the face of a stressor agent was investigated in rats. Ninety-day-old animals were divided into three groups: the control group (only immunological measurements were performed), the foot-shock (FS) (animals individually receiving FS), and the intraspecific aggressive response (IAR) group (animals receiving FS and presenting IAR). For immunological measurements, blood samples were collected promptly at 7 and 15 days after FS or IAR. The FS reduced the total leukocyte amount presented. However, aggressiveness triggered not only reduction of the leukocytes, but also lymphocyte decrease and neutrophil increase. Moreover, an elevation in total leukocytes associated with an increase in the humoral immune response was also observed one week after IAR. In this study, the expression of intraspecific aggressiveness in the face of a stressor seemed to activate the immune system and to potentiate the antigen specific humoral response.

scholarly journals Potential Involvement of lncRNAs in the Modulation of the Transcriptome Response to Nodavirus Challenge in European Sea Bass (Dicentrarchus labrax L.)

Biology ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 165
Author(s):  
Patricia Pereiro ◽  
Raquel Lama ◽  
Rebeca Moreira ◽  
Valentina Valenzuela-Muñoz ◽  
Cristian Gallardo-Escárate ◽  
...  
Keyword(s):  
Immune Response ◽  
Dicentrarchus Labrax ◽  
Head Kidney ◽  
Enrichment Analysis ◽  
Sea Bass ◽  
Integrated Analysis ◽  
European Sea Bass ◽  
Commercial Species ◽  
Number Of Publications ◽  
Systematic Identification

Long noncoding RNAs (lncRNAs) are being increasingly recognised as key modulators of various biological mechanisms, including the immune response. Although investigations in teleosts are still lagging behind those conducted in mammals, current research indicates that lncRNAs play a pivotal role in the response of fish to a variety of pathogens. During the last several years, interest in lncRNAs has increased considerably, and a small but notable number of publications have reported the modulation of the lncRNA profile in some fish species after pathogen challenge. This study was the first to identify lncRNAs in the commercial species European sea bass. A total of 12,158 potential lncRNAs were detected in the head kidney and brain. We found that some lncRNAs were not common for both tissues, and these lncRNAs were located near coding genes that are primarily involved in tissue-specific processes, reflecting a degree of cellular specialisation in the synthesis of lncRNAs. Moreover, lncRNA modulation was analysed in both tissues at 24 and 72 h after infection with nodavirus. Enrichment analysis of the neighbouring coding genes of the modulated lncRNAs revealed many terms related to the immune response and viral infectivity but also related to the stress response. An integrated analysis of the lncRNAs and coding genes showed a strong correlation between the expression of the lncRNAs and their flanking coding genes. Our study represents the first systematic identification of lncRNAs in European sea bass and provides evidence regarding the involvement of these lncRNAs in the response to nodavirus.

scholarly journals Seasonal variations of the humoral immune parameters of European sea bass (Dicentrarchus labrax L.)

2014 ◽  
Vol 39 (2) ◽  
pp. 185-187 ◽  
Author(s):  
Yulema Valero ◽  
Alicia García-Alcázar ◽  
M. Ángeles Esteban ◽  
Alberto Cuesta ◽  
Elena Chaves-Pozo
Keyword(s):  
Seasonal Variations ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
European Sea Bass ◽  
Humoral Immune ◽  
Immune Parameters

scholarly journals Induction of a Protective Immune Response against Viral Nervous Necrosis in the European Sea Bass Dicentrarchus labrax by Using Betanodavirus Virus-Like Particles

2006 ◽  
Vol 80 (20) ◽  
pp. 10201-10207 ◽  
Author(s):  
R. Thiéry ◽  
J. Cozien ◽  
J. Cabon ◽  
F. Lamour ◽  
M. Baud ◽  
...  
Keyword(s):  
Immune Response ◽  
Expression System ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Baculovirus Expression System ◽  
Single Type ◽  
European Sea Bass ◽  
Virus Like Particles ◽  
Viral Nervous Necrosis ◽  
Baculovirus Expression

ABSTRACT Betanodaviruses are causative agents of viral nervous necrosis (VNN), a devastating disease of cultured marine fish worldwide. Virus particles contain a single type of coat protein that spontaneously assembles into virus-like particles (VLPs) when expressed in a baculovirus expression system. In the present study, the immunogenicity of betanodavirus VLPs and the protection they confer against VNN in the European sea bass Dicentrarchus labrax were investigated. Enzyme-linked immunosorbent assay and seroneutralization tests performed on plasma from fish vaccinated intramuscularly with doses as low as 0.1 μg of VLPs indicated that the VLPs elicited the synthesis of specific antibetanodavirus antibodies with neutralizing activity. Moreover, fish vaccinated with VLPs were protected from challenge with live virus. Both the immune response and the protective effect against viral challenge were dose dependent. Reverse transcription-PCR data indicated that higher doses of vaccine also reduced the number of fish containing detectable quantities of betanodavirus RNA on day 30 after challenge. Taken together these data strongly support the hypothesis that VLPs obtained in the baculovirus expression system may represent an effective vaccine against VNN.

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