Effects of synchronising dietary nitrogen and energy supply in diets with a similar carbohydrate composition on rumen fermentation and microbial protein synthesis in sheep

1993 ◽  
Vol 1993 ◽  
pp. 111-111
Author(s):  
L. A. Sinclair ◽  
P. C. Garnsworthy ◽  
J. R. Newbold ◽  
P. J. Buttery
Keyword(s):  
Protein Synthesis ◽  
Rumen Fermentation ◽  
Carbohydrate Composition ◽  
Microbial Protein ◽  
Rumen Microbes ◽  
Microbial Protein Synthesis ◽  
Dietary Nitrogen ◽  
Microbial Nitrogen ◽  
Metabolisable Energy ◽  
Micro Organisms

The recently introduced metabolisable protein system for ruminants (Webster 1992) relates microbial nitrogen production to daily supply of fermentable metabolisable energy and effective rumen degradable protein but does not consider the effect of the pattern of supply of nutrients to rumen microbes on their efficiency and growth. However, synchronising the hourly supply of nitrogen and energy yielding substrates to rumen micro-organisms has been shown to increase the efficiency of microbial protein synthesis (Sinclair et al. 1993). The objective of the current experiment was to examine the effects of synchronising the hourly supply of energy and nitrogen in diets with a similar carbohydrate composition but differing in the rate of protein degradation, on rumen fermentation and microbial protein synthesis in sheep.

Effects of synchronizing the rate of dietary energy and nitrogen release in diets with a similar carbohydrate composition on rumen fermentation and microbial protein synthesis in sheep

1995 ◽  
Vol 124 (3) ◽  
pp. 463-472 ◽  
Author(s):  
L. A. Sinclair ◽  
P. C. Garnsworthy ◽  
J. R. Newbold ◽  
P. J. Buttery
Keyword(s):  
Protein Synthesis ◽  
Wheat Straw ◽  
Rumen Fermentation ◽  
Winter Barley ◽  
Dietary Energy ◽  
Carbohydrate Composition ◽  
Microbial Protein ◽  
Microbial Protein Synthesis ◽  
Cyclical Trend ◽  
Microbial N

SUMMARYThe effects of synchronizing dietary energy and nitrogen supply in diets with a similar carbohydrate composition on microbial protein synthesis and rumen fermentation were examined in sheep. Two diets were formulated to be either synchronous (diet S) or asynchronous (diet A) for the hourly release of nitrogen (N) and energy to the rumen. Diet S contained (g/kg) 425 g wheat straw, 400 g winter barley, 150 grapeseed meal and 25 g minerals/vitamins and diet A contained 505 g wheat straw, 458.5 g winter barley, 11·5 g urea and 25 g minerals/vitamins. Both diets were fed at the rate of 1 kg/day in four equal portions, to four cannulated sheep, in two periods in a change-over design. Rumen ammonia concentrations followed the predicted hourly trend in N degradation with a peak 1 h after feeding of 10 mM for diet S and 16 mM for diet A before falling within 3 h of feeding to 4 ITIM in animals fed either diet. Rumen volatile fatty acid (VFA) concentrations followed the cyclical trend predicted by stoichiometric equations, whilst rumen VFA ratios were more stable than predicted in animals fed either diet. The observed content of rumen degradable protein and organic matter truly degraded in the rumen was similar for both diets. The increase in total CHO digested in the rumen observed with diet A (427 g/kg DM) compared with diet S (364 g/kg DM) can be attributed to the greater content of starch in the asynchronous diet, which had a high degradability. The efficiency of microbial protein synthesis (g N/kg OM truly degraded in the rumen) was 11–20% greater in animals fed the synchronous diet (S) than the asynchronous diet (A). It is concluded that microbial N production was more efficient when dietary energy and N supply were synchronized.

scholarly journals Effect of concentrate supplementation to grass silage diets on rumen fermentation, diet digestion and microbial protein synthesis in growing heifers

1992 ◽  
Vol 1 (2) ◽  
pp. 177-188
Author(s):  
Ilmo Aronen ◽  
Aila Vanhatalo
Keyword(s):  
Protein Synthesis ◽  
Volatile Fatty Acids ◽  
Total Concentration ◽  
Latin Square ◽  
Rapeseed Meal ◽  
Rumen Fermentation ◽  
Microbial Protein ◽  
Rumen Microbes ◽  
Microbial Protein Synthesis ◽  
Grass Silage

A 4 x 4 latin square experiment was carried out with four growing heifers, each with a rumen cannula and a simple T-cannula inserted in the proximal duodenum. The purpose was to study the effects of the supplementation of concentrate to grass silage on rumen fermentation, microbial protein synthesis and digestion of organic matter (OM), fibre components and N. The diets were composed of grass silage alone (S); grass silage and barley (SBU, 50:50 % on dry matter (DM) basis); and grass silage, barley and protein concentrate based either on rapeseed meal (SBR), or meat and bone meal (SBM) (50:40:10). To make the diets isonitrogenous, 23 g of urea was given with the SBU diet. The supplementation of concentrates, irrespective of their type, increased the average rumen ammonia-N and total concentration of volatile fatty acids (VFA) and decreased the molar proportion of acetate. Inclusion of concentrates in the diet had a negative effect on the digestibility of cell wall constituents. The production of microbial protein and the efficiency of microbial protein synthesis were not affected by the diet. It appears, therefore, that the supply of nitrogenous constituents for rumen microbes through ruminally degraded protein was adequate in silage feeding, and that no extra benefit, at the utilized level of application, was gained by the supplementation of any of the concentrates.

Effect of pine-based biochars with differing physiochemical properties on methane production, ruminal fermentation, and rumen microbiota in an artificial rumen (RUSITEC) fed barley silage

2021 ◽  
pp. 1-13
Author(s):  
Paul Tamayao ◽  
Gabriel O. Ribeiro ◽  
Tim A. McAllister ◽  
Kim H. Ominski ◽  
Atef M. Saleem ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Fixed Effects ◽  
Block Design ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Microbial Protein ◽  
Rumen Microbiota ◽  
Alpha And Beta Diversity ◽  
Microbial Protein Synthesis ◽  
Barley Silage

This study investigated the effects of three pine-based biochar products on nutrient disappearance, total gas and methane (CH4) production, rumen fermentation, microbial protein synthesis, and rumen microbiota in a rumen simulation technique (RUSITEC) fed a barley-silage-based total mixed ration (TMR). Treatments consisted of 10 g TMR supplemented with no biochar (control) and three different biochars (CP016, CP024, and CP028) included at 20 g·kg−1 DM. Treatments were assigned to 16 fermenters (n = 4 per treatment) in two RUSITEC units in a randomized block design for a 17 d experimental period. Data were analyzed using MIXED procedure in SAS, with treatment and day of sampling as fixed effects and RUSITEC unit and fermenters as random effects. Biochar did not affect nutrient disappearance (P > 0.05), nor total gas or CH4, irrespective of unit of expression. The volatile fatty acid, NH3-N, total protozoa, and microbial protein synthesis were not affected by biochar inclusion (P > 0.05). Alpha and beta diversity and rumen microbiota families were not affected by biochar inclusion (P > 0.05). In conclusion, biochar did not reduce CH4 emissions nor affect nutrient disappearance, rumen fermentation, microbial protein synthesis, or rumen microbiota in the RUSITEC.

scholarly journals Plant-based strategies towards minimising ‘livestock's long shadow’

2010 ◽  
Vol 69 (4) ◽  
pp. 613-620 ◽  
Author(s):  
Alison H. Kingston-Smith ◽  
Joan E. Edwards ◽  
Sharon A. Huws ◽  
Eun J. Kim ◽  
Michael Abberton
Keyword(s):  
Protein Synthesis ◽  
Ruminal Fermentation ◽  
Microbial Protein ◽  
Secondary Products ◽  
Livestock Farming ◽  
Pasture Grass ◽  
Microbial Protein Synthesis ◽  
Human Food Chain ◽  
Micro Organisms ◽  
Ruminal Digestion

Ruminant farming is an important component of the human food chain. Ruminants can use offtake from land unsuitable for cereal crop cultivation via interaction with the diverse microbial population in their rumens. The rumen is a continuous flow fermenter for the digestion of ligno-cellulose, with microbial protein and fermentation end-products incorporated by the animal directly or during post-ruminal digestion. However, ruminal fermentation is inefficient in capturing the nutrient resource presented, resulting in environmental pollution and generation of greenhouse gases. Methane is generated as a consequence of ruminal fermentation and poor retention of ingested forage nitrogen causes nitrogenous pollution of water and land and contributes to the generation of nitrous oxide. One possible cause is the imbalanced provision of dietary substrates to the rumen micro-organisms. Deamination of amino acids by ammonia-producing bacteria liberates ammonia which can be assimilated by the rumen bacteria and used for microbial protein synthesis. However, when carbohydrate is limiting, microbial growth is slow, meaning low demand for ammonia for microbial protein synthesis and excretion of the excess. Protein utilisation can therefore be improved by increasing the availability of readily fermentable sugars in forage or by making protein unavailable for proteolysis through complexing with plant secondary products. Alternatively, realisation that grazing cattle ingest living cells has led to the discovery that plant cells undergo endogenous, stress-mediated protein degradation due to the exposure to rumen conditions. This presents the opportunity to decrease the environmental impact of livestock farming by using decreased proteolysis as a selection tool for the development of improved pasture grass varieties.

Measurement of microbial protein synthesis in Iranian buffalo rumen (Mazandran Province) by purine derivatives excretion method

2007 ◽  
Vol 2007 ◽  
pp. 216-216
Author(s):  
Yashar Vakil Faraji ◽  
Mojtaba Zahedifar ◽  
Jafari Khorshidi Kaveh
Keyword(s):  
Protein Synthesis ◽  
Uric Acid ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Mazandaran Province ◽  
Rumen Microbes ◽  
Microbial Protein Synthesis ◽  
North Of Iran ◽  
Swamp Buffalo ◽  
Protein Supply

Rumen microbes are rich in nucleic acid: around 18% of total nitrogen is present on nucleic acids or 11% in purines. Rumen microbes constitue the major source of protein supply to the ruminant. The purines from the rumen microbes are metabolized and excreted in the urine as their end products: hypoxanthine, xanthine, uric acid and allantoin. In buffalo and cattle because of high xanthine oxidase activity in intestine and blood, hypoxanthine and xanthine convert to uric acid therefore only uric acid and allantoin excreted in urine way (Chen, X. B., Ørskov, E. R., 2003). This research carried out to use excretion of purine derivatives namely allantoin and uric acid as a parameter to estimate the microbial protein synthesis in the rumen of native swamp buffalo in north of iran, Mazandaran Province.

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