Preparation of Helminths For Scanning Electron Microscopy

1973 ◽  
Vol 31 ◽  
pp. 438-439
Author(s):  
Robert W. Weise
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Room Temperature ◽  
Parasitic Nematodes ◽  
Live Animal ◽  
Transmission Electron ◽  
Critical Point Drying ◽  
Scanning Electron

The role that scanning electron microscopy (SEM) is playing in descriptive helminthology is becoming more apparent in the literature. However, the majority of papers on the SEM of helminths have used conventional or modified light microscope techniques of fixation and dehydration, and not established SEM techniques in which freeze- and critical point-drying are routinely used. The present investigation was undertaken to examine the applicability of modified scanning and transmission electron microscope techniques for the preparation of certain helminths for SEM.Method I.– Live animal-parasitic nematodes were fixed in 6% phosphate buffered glutaraldehyde for 24 hr at room temperature.

Paraliomyces lentiferus: an ultrastructural study of a little-known marine ascomycete

1992 ◽  
Vol 70 (11) ◽  
pp. 2223-2232 ◽  
Author(s):  
S. J. Read ◽  
S.-Y. Hsieh ◽  
E. B. G. Jones ◽  
S. T. Moss ◽  
H. S. Chang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Ultrastructural Study ◽  
Republic Of China ◽  
Mucilaginous Sheath ◽  
Transmission Electron ◽  
Scanning Electron

A collection of Paraliomyces lentiferus from Taiwan, Republic of China, is compared with that of the type description and examined at both scanning and transmission electron microscope levels as part of our review of the taxonomy of the marine Ascomycotina. Particular attention was devoted to the structure of the ascospore appendage. The ascospore wall comprises a mesosporium, an episporium, and a mucilaginous sheath (exosporium?) In addition, there is a single, gelatinous, lateral appendage adjacent to the central septum. The appendage comprises electron-opaque fibrils that in immature ascospores are connected to the ascospore wall via fine electron-opaque strands and larger electron-opaque aggregates of material. The origin of the appendage is discussed. Key words: ascospore, attachment, marine ascomycete, scanning electron microscopy, spore appendage, transmission electron microscopy.

Copper Localization in Cirrhotic Rat Liver by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 38-39 ◽  
Author(s):  
J. C. Russ ◽  
E. McNatt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Copper Acetate ◽  
Lead Citrate ◽  
Dense Bodies ◽  
Transmission Electron ◽  
Electron Mode ◽  
Scanning Electron

In order to study the retention of copper in cirrhotic liver, rats were made cirrhotic by carbon tetrachloride inhalation twice weekly for three months and fed 0.2% copper acetate ad libidum in drinking water for one month. The liver tissue was fixed in osmium, sectioned approximately 2000 Å thick, and stained with lead citrate. The section was examined in a scanning electron microscope (JEOLCO JSM-2) in the transmission electron mode.Figure 1 shows a typical area that includes a red blood cell in a sinusoid, a disse, and a portion of the cytoplasm of a hepatocyte which contains several mitochondria, peribiliary dense bodies, glycogen granules, and endoplasmic reticulum.

Novel Methods for Preparation and Surface Treatment of Nano-CaCO3

2013 ◽  
Vol 750-752 ◽  
pp. 336-339
Author(s):  
Fa Chao Wu ◽  
Teng Fei Shen
Keyword(s):  
Electron Microscopy ◽  
Heat Treatment ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Particle Size ◽  
Fourier Transform ◽  
Room Temperature ◽  
Transmission Electron ◽  
Reduce Energy Consumption ◽  
Scanning Electron

In this work, CaCO3 nanoparticles have been synthesized via heat-treatment of a new precursor. Effect of calcinations temperature on particle size has been investigated. The products were characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). nanoCaCO3 was modified using chloroform as solvent and fatty acid as modifier atroom temperature. The advantage of this modification is that it can be proceed at room temperature and it can reduce energy consumption.

Taxonomic Identification of Rhabdochona Species Found in Oncorhynchus Clarki Using Scanning Electron Microscopy

1998 ◽  
Vol 4 (S2) ◽  
pp. 1148-1149
Author(s):  
D. Young ◽  
R.A. Heckmann ◽  
J. S. Gardner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Digestive Tract ◽  
Cutthroat Trout ◽  
Parasitic Nematodes ◽  
Buffer System ◽  
Taxonomic Identification ◽  
Critical Point Drying ◽  
Cacodylate Buffer ◽  
Scanning Electron

Adult Rhabdochona nematodes, commonly parasitizing fish, were present in the digestive tracts of cutthroat trout in Little Cottonwood Creek, Utah. Cutthroat trout, Oncorhyncus clarki, are known to serve as both intermediate and definitive hosts for parasitic nematodes. The larval stage parasitizes almost any tissue of its host, but the adult is always found in the digestive tract. Due to the lack of key morphological features, scanning electron microscopy (SEM) was used to identify specific structures leading to the nematode's taxonomic identification.Cutthroat trout were obtained using a rod and reel and were dissected the same day. Nematodes were present in all 12 cutthroat trout residing in all parts of the digestive tract. The nematodes, Rhabdochona sp., were prepared for SEM using the following procedures. First, the parasites were fixed in 2% buffered glutaraldehyde, washed in sodium cacodylate buffer, and post fixed in a 1% solution of osmium tetroxide. The samples were then washed in the same buffer system and dehydrated through a graded alcohol series. Critical-point-drying removed the remaining fluids. Finally, the nematodes were placed on specimen stubs, sputter coated with gold, and each specimen examined with a JOEL-840 high resolution scanning electron microscope with micrographs taken at varying magnifications.

Synthesis of Silicon Carbide Nanoparticles from Carbon Nanotubes

2012 ◽  
Vol 602-604 ◽  
pp. 183-186 ◽  
Author(s):  
Jing Liu ◽  
Rong Wu ◽  
Jin Li ◽  
Yan Fei Sun ◽  
Ji Kang Jian
Keyword(s):  
Electron Microscopy ◽  
Carbon Nanotubes ◽  
Scanning Electron Microscopy ◽  
Silicon Carbide ◽  
Room Temperature ◽  
Sic Nanoparticles ◽  
X Ray ◽  
Transmission Electron ◽  
Cubic Silicon Carbide ◽  
Scanning Electron

In this paper, we report the synthesis of cubic silicon carbide (3C-SiC) nanoparticles by direction reaction of silicon powders and carbon nanotubes. The as-prepared SiC nanoparticles were characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy and Raman scattering at room temperature. The possible growth mechanism is proposed.

Preparation of Hollow Nickel Ferrite Microspheres and their Magnetic Properties

2014 ◽  
Vol 1035 ◽  
pp. 488-491
Author(s):  
Jing Jing Li ◽  
Yun Zhao ◽  
Han Sheng Li ◽  
Qin Wu ◽  
Qing Ze Jiao
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nickel Ferrite ◽  
Room Temperature ◽  
Solvothermal Method ◽  
Magnetization Measurement ◽  
Vibrating Sample Magnetometer ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

Hollow nickel ferrite microspheres with a diameter of about 1.5 to 2.5 μm were synthesized using an emulsion-based solvothermal method in combination with calcination at 550°C. The structures and morphologies of the nickel ferrite microspheres were characterized using an X-ray diffractometer, a transmission electron microscopy and a field emission scanning electron microscopy. Magnetization measurement was carried out using a vibrating sample magnetometer at room temperature. The saturation magnetization and coercivity of nickel ferrite microspheres could reach 19.41 emu/g and 202.28 Oe, respectively. Hollow nickel ferrite microspheres might be used as catalysts, magnetic materials and microwave absorbers.

Scanning Electron Microscopy of soft-bodied tephritid larvae

1990 ◽  
Vol 48 (3) ◽  
pp. 490-490
Author(s):  
R. Paul ◽  
V. C. Kapoor
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Deionized Water ◽  
Soft Body ◽  
Black And White ◽  
Critical Point Drying ◽  
White Film ◽  
Ethanol Series ◽  
Scanning Electron

The soft body of dipteran larva has been posing a difficulty for entomologist to go for scanning electron microscopy. Dehydration by the conventional methods results in crumpling of the larval integument thereby distorting the minute details. The cuticle is impermeable to various types of fixatives and its puncturing with needle causes severe distortion of structure due to haemoiymphloss. To avoid this a new method was developed to prepare specimen for scanning electron microscopy. The specimen was kept in doubled is tilled deionized water at 60°C for half an hour before further treatment. It was sonicated in mild detergent (Sodium bicarbonate) for 30 sec. It was again washed with deionized water and specimen was placed in modified super skiper's solution (Grodowitz et al., 1982) and then rinsed twice in Carl's solution, after dehydration through ethanol series, critical point drying was done. Specimen was coated with gold pulludium and photographed by using JEM - 1200 EX (Jeol) electron microscope and Indu Panchromatic 125 ASA black and white film.

Fabrication of g-C3N4/Y-TiO2 Z-scheme heterojunction photocatalysts for enhanced photocatalytic activity

2021 ◽  
Author(s):  
SongSik Pak ◽  
KwangChol Ri ◽  
Chenmin Xu ◽  
Qiuyi Ji ◽  
Dunyu Sun ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fourier Transform ◽  
Photocatalytic Activity ◽  
Electron Microscope ◽  
Photoelectron Spectroscopy ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

The g-C3N4/Y-TiO2 Z-scheme heterojunction photocatalysts were successfully synthesized. The powder X-ray diffraction, scanning electron microscopy, high-resolution transmission electron microscope, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy were used for...

A technique for preparing Beauveria spp. for scanning electron microscopy

1980 ◽  
Vol 58 (15) ◽  
pp. 1700-1703 ◽  
Author(s):  
E. C. Quattlebaum ◽  
G. R. Carner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Freeze Drying ◽  
Osmium Tetroxide ◽  
Air Drying ◽  
Preparation Methods ◽  
Critical Point Drying ◽  
Scanning Electron

Vapor fixation for 96 h with 1% osmium tetroxide (OsO4) and 3–4 days air drying produced distortion-free specimens of Beauveria spp. for examination with the scanning electron microscope. A combination of 4 h OsO4 vapor fixation and freeze-drying also reduced disruption satisfactorily but specimens were not as well preserved as with the first method. Preparation methods that were ineffective in preventing collapse of hydrophilic structures were Cling Free® sprayed on specimens prior to examination, freeze-drying, critical-point drying (of unfixed material), and vapor fixation with glutaraldehyde.

Two-Step Fabrication of Nano-PbS on Peacock Feathers Inspired by a Hair-Dyeing Method Used in Ancient Egypt

2013 ◽  
Vol 364 ◽  
pp. 737-741
Author(s):  
Xiao Wei Liu ◽  
Jia Jun Gu ◽  
Fang Yu Zhang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Ancient Egypt ◽  
X Ray Diffraction ◽  
X Ray ◽  
Whole Process ◽  
Transmission Electron ◽  
Dyeing Method ◽  
Scanning Electron

A rapid method towards nanoPbS on peacock feathers was reported and this is inspired by a hair-dyeing technology used in Ancient Egypt thousands of years ago. Original peacock feather was sulfhydrylated by 2, 3-dimercaptosuccinic acid (DMSA) dissolved in alcohol to enhance reaction sites, and then was immersed in the saturated PbO solution in calcium hydroxide and got the PbS peacock feather. The whole process is only two steps and could be completed within two hours. The morphology and structures of the sample were measured by the X-ray diffraction (XRD), Scanning electron microscopy (SEM), and transmission electron microscope (TEM) and results showed that the structure of original peacock feather was well duplicated. Compared with previous works, this method is faster and more efficient and thus has potentials to fabricate other functional sulfides.

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