Expression of laminin and beta-1 integrin in embryonic, neonatal, and adult rat heart

1991 ◽  
Vol 49 ◽  
pp. 182-183
Author(s):  
R.L. Price ◽  
T.K. Borg ◽  
L. Terracio ◽  
M. Nakagawa
Keyword(s):  
Temporal Expression ◽  
Qualitative And Quantitative ◽  
Adult Rat ◽  
Cytoplasmic Face ◽  
Extracellular Matrix Components ◽  
Basement Membrane Component ◽  
Beta 1 Integrin ◽  
Quantitative Changes ◽  
Cytoskeletal Elements ◽  
External Face

Little is known about the temporal expression of extracellular matrix components (ECM) and its receptors during development of the heart. Recent reports have shown that ECM components undergo both qualitative and quantitative changes during development, and it is believed that ECM components are important in the regulation of cell migration and cell:cell and cell:ECM recognition and adhesion.Integrins are transmembrane glycoproteins which bind several ECM components on their external face and cytoskeletal elements on the cytoplasmic face. Laminin is a basement membrane component which has been recognized as an important site for cell adhesion. Both the integrins and laminin are expressed early in development and continue to be expressed in the adult heart. With their documented roles in cell recognition, and cell:cell and cell:ECM migration and adhesion these proteins appear to be important components in development of the heart, and their temporal expression may play a pivotal role in morphogenesis and myofibrillogenesis of the heart.

Functional MR Imaging of the Motor Cortex in Active and Passive Movement: Qualitative and Quantitative Changes

2002 ◽  
Vol 46 (5) ◽  
pp. 425
Author(s):  
Ki Bong Yu ◽  
Myung Kwan Lim ◽  
Hyung Jin Kim ◽  
Jun Soo Byun ◽  
Young Kook Cho ◽  
...  
Keyword(s):  
Motor Cortex ◽  
Mr Imaging ◽  
Passive Movement ◽  
Qualitative And Quantitative ◽  
Functional Mr Imaging ◽  
Quantitative Changes

STRUCTURE AND DYNAMICS OF ENVIRONMENTAL PARAMETERS OF HUMAN INTESTINAL MICROBIOME IN DIFFERENT CLIMATIC AND ECOLOGICAL ZONES OF ST. PETERSBURG

2020 ◽  
pp. 31-36
Author(s):  
Bugero N.V. ◽  
Ilyina N.A. ◽  
Aleksandrova S.M.
Keyword(s):  
Environmental Parameters ◽  
Intestinal Microbiome ◽  
Ecological Niches ◽  
Horizontal Structure ◽  
Ecological Zones ◽  
Qualitative And Quantitative ◽  
Symbiotic Relationships ◽  
Structure And Dynamics ◽  
Quantitative Changes ◽  
Micro Organisms

In order to understand the structure and dynamics of symbiotic relationships of human intestinal biotope micro-organisms, taxonomic constancy indices, the degree of contagion of the biocenosis under study and floristic significance were investigated, which made it possible to detect qualitative and quantitative changes in the microecology of the biotope being studied in persons living in different ecologically heterogeneous territories of Saint Petersburg. The contagiosity index estimated the distribution of species in space. Persons living in the ecologically disadvantaged Kirov district of the city have been found to show a reliable increase in this indicator for the obligate microflora: bifido and lacto bacteria, intestinal columns and bacteroids, compared to the resort district, which is considered a relatively favourable area for residence. On the contrary, there has been a reliable decrease in this indicator in the group of opportunistic micro-organisms (fungi of the genus Candida, staphylococcus, clostridium, etc.), resulting in the liberation of ecological niches successfully occupied by transient flora. This ratio reveals an imbalance of participation in the horizontal structure of the intestine ecosystem of the main symbiotes and representatives of the transient flora. To analyse the structure of symbiotic relationships, the constancy indices that form the microflora of the individuals of the groups studied were investigated. The analysis of the data obtained suggested that the dominant species in both groups were optional-anaerobic bifido and lactobacteria and oblique-anaerobic bacteroids. It should be noted, however, that in the residents of the dysfunctional Kirovsky district, against the background of the reduced constancy of the normal flora, opportunistic micro-organisms have entered. The floral significance index was also declining in the obligate flora of this group and increased for opportunistic species. Thus, the study of the environmental characteristics of the intestinal biotope using different ecological parameters for persons living in different technogenic load regions of St. Petersburg has shown that in the Kirov district with an unfavourable environmental situation there are compensated qualitative and quantitative changes in the intestinal microbiocenosis, resulting in a change in the hierarchy in the overall structure of micro-organisms.

Changes in Seryl-tRNA Formative in Developing Chick Muscle

1974 ◽  
Vol 52 (10) ◽  
pp. 838-844 ◽  
Author(s):  
Mark Nwagwu ◽  
John Lianga
Keyword(s):  
Muscle Protein ◽  
Muscle Protein Synthesis ◽  
Deae Cellulose ◽  
The Other ◽  
Embryonic Chick ◽  
Qualitative And Quantitative ◽  
Embryonic Muscle ◽  
Quantitative Changes ◽  
The One

As a prelude to an analysis of the dependence of muscle protein synthesis on aminoacyl tRNA's, we have investigated the rates of seryl-tRNA formation, in vitro, by aminoacylating systems isolated from 11-, 14-, and 17-day chick embryonic muscle. The results show that the combination of 14-day tRNA and 14-day aminoacyl synthetase is the most efficient in seryl-tRNA formation. We have also studied the qualitative and quantitative changes in seryl-tRNA prepared from 11-, 14-, and 17-day embryonic chick muscle by chromatography of seryl-tRNA on benzoylated DEAE-cellulose columns. The results show that, although there are no qualitative differences in the chromatographic patterns of seryl-tRNA from the different ages, there are significant quantitative differences between the patterns for 11-day and 17-day seryl-tRNA on the one hand, and the pattern for 14-day seryl-tRNA on the other.

ANALYSIS OF PROTEOGLYCAN GENE EXPRESSION IN CONNECTIVE TISSUES BY SEMI-QUANTITATIVE RT-PCR

2001 ◽  
Vol 05 (02) ◽  
pp. 79-88
Author(s):  
K. Dobra ◽  
A. Hjerpe
Keyword(s):  
Rt Pcr ◽  
Connective Tissues ◽  
Surrounding Matrix ◽  
Cell Proliferation And Differentiation ◽  
Extracellular Matrix Components ◽  
Proliferation And Differentiation ◽  
The Matrix ◽  
Quantitative Changes ◽  
Regulatory Functions ◽  
Multiple Samples

Proteoglycans (PGs) are cell-membrane and extracellular matrix components with a wide variety of different functions. In the matrix, they are mainly of structural importance, although some of them have been ascribed specific regulatory functions, such as in the assembly of collagen fibers. PGs on the cell surface act as essential modulators of specific ligand-binding reactions, involving interactions between adjacent cells and between cells and surrounding matrix. Through these interactions they participate in different processes, including cell proliferation and differentiation. Qualitative and quantitative changes in PG expression can therefore be associated with various physiological and pathological conditions. We have optimized the conditions for semi-quantitative evaluation of proteoglycan expression by RT-PCR reaction, using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as reference gene. The relative fluorescence of analyte to reference amplimers can — within certain limits — be used to estimate the amount of target RNA and allows direct comparison of multiple samples. The profile of PG expression obtained in this way can be used to extend our current understanding of the possible functions that can be associated with these complex molecules.

scholarly journals Dynamics of Microbial Indicators in Waste Water

2017 ◽  
Vol 74 (2) ◽  
pp. 116
Author(s):  
Vlad STOIAN ◽  
Roxana VIDICAN ◽  
Ioan ROTAR ◽  
Florin PĂCURAR
Keyword(s):  
Abiotic Factors ◽  
Specific Response ◽  
Filamentous Bacteria ◽  
Qualitative And Quantitative ◽  
External Pressures ◽  
Quantitative Changes ◽  
Water Ecosystems ◽  
Microbial Groups ◽  
Foam Production

Water ecosystems are constantly under the pressure of a high number of biotic and abiotic factors, and their action leads to the occurrence of fluctuations in species and populations. The specific response of organisms to external pressures leads to the reorganization of ecosystems, with qualitative and quantitative changes. The aim is to identify the microbial groups with the highest potential in the waste waters. Data were collected during 2015-16 in Bistrita. Identifying the bioindicators quality of microorganisms and their importance in ecosystems was done by comparing them with international databases. The use of microorganisms as bioindicators involves knowing the requirements for one or more environmental variables. The concept refers to the ability of microorganisms to accumulate pollutants. Flagellates are tolerant to acidity and react quickly and with sensitivity to environmental stress by inhibiting their movement. The multiplication of flagellates and filamentous bacteria is manifested through water disturbance and foam production.

Role of Extracellular Matrix Components in Adult Rat Adrenal Gland Homeostasis

2004 ◽  
Vol 30 (4) ◽  
pp. 609-610
Author(s):  
Shirley Campbell ◽  
Mélissa Otis ◽  
Nicole Gallo‐Payet ◽  
Marcel Daniel Payet
Keyword(s):  
Extracellular Matrix ◽  
Adrenal Gland ◽  
Adult Rat ◽  
Extracellular Matrix Components ◽  
Matrix Components
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