Preliminary investigation of fiber ultrastructure after enzymatic digestion

Author(s):  
Janet H. Woodward ◽  
D. E. Akin ◽  
F. E. Barton

Knowledge on the chemical and physical structure of fiber is essential in determining forage quality. Current methods provide empirical results on fiber using chemical extractants. This paper is a preliminary report on methods to redefine fiber based on physiological (biochemical) procedures. TEM was employed to evaluate the relationship of fiber with specific enzymatic treatments.A modified AOAC Total Dietary Fiber Determination was used to prepare one group of insoluble fibers from two legumes of different nutritional quality. Three millimeter wedged-shaped leaflet sections of alfalfa (Medicago sativa L.) and 1-76 lespedeza [Sericea cuneata (Dumont)] were incubated stepwise with: (1) heat stable α amylase, pH 6.0, for h at 100°C; (2) protease, pH 7.5, for h at 60°C; and (3) amyloglucosidase, pH 4.5, for h at 60°C. All leaflet sections were retrieved and incubated for 24 h at 37°C with either: (1) 1% (w/v) cellulase in 0.05 M sodium acetate buffer, pH 5.0; (2) 10% (w/v) hemicellulase in 0.05 M acetate buffer, pH 5.5; or (3) 1% cellulase and 10% hemicel1ulase in the acetate buffer.

2019 ◽  
Vol 14 (4) ◽  
pp. 251-263
Author(s):  
Daniel J. Carabellese ◽  
Michael J. Proeve ◽  
Rachel M. Roberts

Purpose The purpose of this paper is to explore the relationship of two distinct variants of dispositional shame (internal and external shame) with collaborative, purpose-driven aspects of the patient–provider relationship (working alliance) and patient satisfaction. The aim of this research was to conduct a preliminary investigation into the relevance of dispositional shame in a general healthcare population. Design/methodology/approach In total, 127 community members (mean age 25.9 years) who reported that they had regularly seen a GP over the past year were recruited at an Australian university. Participants were asked to reflect on their relationship with their GP, and completed instruments assessing various domains of shame, as well as working alliance and patient satisfaction. Findings Non-parametric correlations were examined to determine the direction and strength of relationships, as well as conducting mediation analyses where applicable. Small, negative correlations were evident between external shame and working alliance. Both external and internal shame measures were also negatively correlated with patient satisfaction. Finally, the relationship of external shame to patient satisfaction was partially mediated by working alliance. Practical implications Both the reported quality of patient–provider working alliance, and level of patient satisfaction are related to levels of dispositional shame in patients, and working alliance may act as a mediator for this relationship. Originality/value The findings from this preliminary study suggest that internal and external shame are important factors to consider in the provision of medical care to maximise the quality of patient experience and working alliance.


1969 ◽  
Vol 15 (12) ◽  
pp. 1409-1413 ◽  
Author(s):  
C. P. Eng ◽  
J. A. Hindmarsh ◽  
J. F. Morgan

Immunodiffusion and hemagglutination tests have demonstrated that antiserum against Ehrlich ascites fluid is active against tissue saline extracts and erythrocytes of the mouse, rat, guinea pig, hamster, and cat. Detailed studies have shown that cat liver extract shares a common antigen present in the Ehrlich ascites cell, normal mouse liver, and guinea pig kidney. Enzyme susceptibility and heat stability tests have indicated that the cat heterophile antigen is heat stable and protein in nature. The relationship of this new heterophile antigen with other identified heterophile antigens is discussed.


2013 ◽  
Vol 842 ◽  
pp. 192-195
Author(s):  
Zhao Xi Wu ◽  
Gui Cui Fu ◽  
Han Tian Gu

Considering physical mechanisms, an electro-thermal effect model of GaAs pseudomorphic high electron mobility transistor (PHEMT) was proposed. The model included PAD capacitances, intrinsic elements and extrinsic elements, which represented the relationship of performance parameters to physical structure and temperature. By using this model, the effects of temperature to devices performance parameters could be analyzed, and the device's structure and materials' parameters could be optimized. A typical GaAs PHEMT was simulated in the EDA software. Thus, DC I-V characteristics and small-signal S parameters of PHEMT under varied temperatures could be given.


2016 ◽  
Vol 27 (3) ◽  
pp. 610-624
Author(s):  
Milutin Stojanovic

In the last two decades the old debate concerning reality of science shifted from questions regarding scientific entities to questions regarding scientific structures. I will present and assess advantages and drawback of this new realists? focus on structures, and at the same time analyze the wider picture of development of the scientific realism. The structural realism will be tackled in the form encountered in works of John Worrall and James Ladyman. Special attention will be devoted to the relationship of their solutions to the argument based on the scientific revolutions - the pessimistic meta-induction. I will argue that these realist?s strategies are not sufficiently convincing to steer us to make a leap in ontology and presume the existence of meta-physical structure (regardless of the question is it scientifically relevant) - in the first place because neither one of them manages to satisfactorily identify a structure, however general, which accumulates in the scientific-theory change.


Paleobiology ◽  
1980 ◽  
Vol 6 (02) ◽  
pp. 146-160 ◽  
Author(s):  
William A. Oliver

The Mesozoic-Cenozoic coral Order Scleractinia has been suggested to have originated or evolved (1) by direct descent from the Paleozoic Order Rugosa or (2) by the development of a skeleton in members of one of the anemone groups that probably have existed throughout Phanerozoic time. In spite of much work on the subject, advocates of the direct descent hypothesis have failed to find convincing evidence of this relationship. Critical points are:(1) Rugosan septal insertion is serial; Scleractinian insertion is cyclic; no intermediate stages have been demonstrated. Apparent intermediates are Scleractinia having bilateral cyclic insertion or teratological Rugosa.(2) There is convincing evidence that the skeletons of many Rugosa were calcitic and none are known to be or to have been aragonitic. In contrast, the skeletons of all living Scleractinia are aragonitic and there is evidence that fossil Scleractinia were aragonitic also. The mineralogic difference is almost certainly due to intrinsic biologic factors.(3) No early Triassic corals of either group are known. This fact is not compelling (by itself) but is important in connection with points 1 and 2, because, given direct descent, both changes took place during this only stage in the history of the two groups in which there are no known corals.


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