Ultrastructural study of a granular cell tumor from forearm

Granular cell tumors are frequently reported in different body locations. Some authors believed it to be a neoplasm derived from skeletal muscle and others propose the origin of these lesions are from fibroblasts, histocytes, mesenchymal cell, neuron or Schwann cells. So far the tumor cells origin is still controversial. The purpose of this report is to present a case showing the cellular fine structures and to propose a possible origin of the tumor cells.A tumor mass (5 x 3 cm) from the left forearm of a 49 year old white male was removed, cut into small pieces (about 1 mm3), immediately fixed in 4 percent glutaraldehyde in phosphate buffer for 2 hours, and then post fixed in 1 percent buffered osmium tetroxide for 1 hour. After dehydration, tissues were embedded in Epon 812.

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Granular Cell Tumor (Myoblastoma) of RAT Brain

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053814 ◽

1982 ◽

Vol 40 ◽

pp. 230-231

Author(s):

Yvonne White ◽

Winslow Sheldon ◽

Terrell Hoage ◽

Robert West

Keyword(s):

Granular Cell Tumor ◽

Molecular Layer ◽

Dorsal Surface ◽

Granular Cell ◽

Cell Tumor ◽

Sprague Dawley ◽

Tumor Mass ◽

Neural Origin ◽

The Brain ◽

Gross Examination

Granular cell myoblastoma is a rare tumor of which the nature and origin has been controversial. The cytogenesis of this tumor has been suggested as a neoplastic derivation from adult or embryonic muscle tissue, a histiocytic storage phenomenon, of neural origin and as a neoplasm derived from Schwann cells.This report presents the ultrastructure of a spontaneously occurring granular cell tumor from the brain of a 22 month old female Sprague- Dawley rat. Gross examination revealed an oval, gray external mass 3x4 mm in size and located on the cerebellar dorsal surface. This tumor was connected by a narrow stalk to a tumor mass located in the molecular layer of the cerebellum. The external tumor and the stalk were excised for electron microscopy (EM) and the remaining tumor mass, brain, and stalk were processed for light microscopy (LM).

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Giant Granular Cell Tumor of the Suprasellar Area; Immunocytochemical and Electron Microscopic Studies

Neurosurgery ◽

10.1227/00006123-198408000-00017 ◽

1984 ◽

Vol 15 (2) ◽

pp. 246-251 ◽

Cited By ~ 28

Author(s):

Boleslaw H. Liwnicz ◽

Regina G. Liwnicz ◽

Stephen J. Huff ◽

Bert H. McBride ◽

...

Keyword(s):

Tumor Cells ◽

Granular Cell Tumor ◽

Periodic Acid ◽

Granular Cell ◽

Cell Tumor ◽

Electron Microscopic ◽

Periodic Acid Schiff ◽

Computed Tomographic ◽

Suprasellar Region ◽

Microscopic Studies

Abstract We describe a case of a granular cell tumor (GCT) of the suprasellar region with an 11-year history in a 26-year-old woman. The computed tomographic scan showed a midline, contrast-enhancing, noncalcified mass. The biopsy was diagnosed as GCT. The tumor was treated with radiation therapy. At necropsy, a large, homogeneous GCT surrounded by gliosis was found. The tumor cells were filled with granules positive for periodic acid-Schiff, diastase-resistant. The cells did not contain glial fibrillary acidic protein or S-100 protein. Electron microscopy showed tumor cells filled with innumerable lysosomal structures. No intermediate filament was found within the cytoplasm. The tumor cells were not surrounded by a basement membrane. Based on this study and on our review of the literature, the suggestion that GCT has a multicellular origin is upheld.

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Multifocal Abrikossoff's granular cell tumor of the oesophagus: Case report

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh0810533r ◽

2008 ◽

Vol 136 (9-10) ◽

pp. 533-537 ◽

Cited By ~ 1

Author(s):

Tomislav Randjelovic ◽

Zorica Stojsic ◽

Jasna Gacic ◽

Darko Babic ◽

Miodrag Stojiljkovic ◽

...

Keyword(s):

Tumor Cells ◽

Granular Cell Tumor ◽

Pathological Finding ◽

Granular Cell ◽

Soft Tissue Tumors ◽

Posterior Mediastinum ◽

Cell Tumor ◽

Ki 67 ◽

Luminal Narrowing ◽

Operative Specimen

INTRODUCTION Granular cell tumors, relatively uncommon soft tissue tumors, have been a matter of debate among pathologists regarding histogenesis for a long time. Less common locations are in the aerodigestive tract including the oesophagus. CASE OUTLINE We have recently treated a rare case, a 37-year old male, who was admitted due to dysphagia and a painful swallow with occasional pharyngo-nasal regurgitation followed with a mild loss of weight. Standard clinical examination including X-ray chest, ECG and laboratory tests did not show pathological findings. Barium contrast oesophagography demonstrated multiple ovoid defects in the wall of the oesophagus. CT scan of the chest confirmed luminal narrowing owing to the tumor of the upper oesophagus. Upper endoscopy showed unusual multifocal nodular lesions alongside the oesophageal axis covered by smooth mucosa. A primary biopsy specimen taken from the largest nodules confirmed an unusual pathological finding of the granular cell tumor. Subtotal, transpleural oesophagectomy was performed and reconstruction was derived by long colon segment interposition through the posterior mediastinum. The postoperative course was uneventful. The operative specimen consisted of four ovoid tumors alongside the oesophagus (the greatest diameter 0.5-1.8, average 1.25). All verified tumors histologicaly consisted of a spindle-shaped or polygonal cells containing small and large eosinophilic granules and central nuclei. Most tumor cells showed strongly positive immunohistochemical staining for S-100 protein. These tumor cells were partially positive for p-53 and Ki-67. No lymph node metastases were detected histologically. CONCLUSION Multifocal granular cell tumor of the oesophagus is an unusual finding with low incidence, and rarely caused symptoms. Pathological features and multiplicity of such tumors emphasized malignant predisposition requiring surgical resection of the oesophagus.

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Granular Cell Tumor Presenting as a Tracheal Mass in a 17-year-old Female

Philippine Journal of Otolaryngology Head and Neck Surgery ◽

10.32412/pjohns.v23i1.777 ◽

2008 ◽

Vol 23 (1) ◽

pp. 39-40

Author(s):

Jose M. Carnate ◽

Audie G. Silva

Keyword(s):

Tumor Cells ◽

Granular Cell Tumor ◽

S100 Protein ◽

Granular Cell ◽

Surgical Excision ◽

Cell Tumor ◽

Granular Cytoplasm ◽

History Of ◽

Infiltrative Growth Pattern ◽

One Year

Granular cell tumors involving the trachea are rare. We present the case of a seventeen year old female with a one year history of gradually worsening dyspnea necessitating a tracheotomy. A suprastomal intraluminal tracheal mass was excised. Histologic sections (Figure 1) show a poorly circumscribed neoplasm infiltrating through the tracheal cartilage. It is composed of polygonal to somewhat elongated tumor cells that have small, dark nuclei. The cytoplasm is ample, eosinophilic and strikingly granular in quality. The cell borders are ill-defined creating a `syncytial’ pattern of dark nuclei scattered in a sea of granular cytoplasm. The diagnosis was a granular cell tumor. Immunohistochemistry (Figure 2) revealed strong, diffuse cytoplasmic positivity for S100 protein, attesting to its neural crest histogenesis. The infiltrative growth pattern may momentarily raise the question of malignancy but this is dispelled by awareness that infiltration is the natural history for all granular cell tumors, benign or malignant. Histologically, malignancy is diagnosed if three or more of the following are present: necrosis, spindling of tumor cells, vesicular nuclei with large nucleoli, greater than 2 mitoses per ten high power fields, high nucleus-to-cytoplasm ratio and nuclear pleomorphism. None was present in our case. Surgical excision remains the mainstay of treatment.

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Bladder Malignant Granular Cell Tumor With EP300 Gene Mutation: A Case Report and Literature Review

10.21203/rs.3.rs-337088/v1 ◽

2021 ◽

Author(s):

Jian Huang ◽

Di Zhu ◽

Xinle Ren ◽

Bing Huang

Keyword(s):

Literature Review ◽

Tumor Cells ◽

Gene Mutation ◽

Granular Cell Tumor ◽

Granular Cell ◽

Posterior Wall ◽

Cell Tumor ◽

Proliferation Index ◽

Pathological Examination ◽

Malignant Granular Cell Tumor

Abstract Background Malignant granular cell tumor (GCT) is extremely rare. Malignant GCT with EP300 gene mutation in the bladder has not been reported in the literature.Case presentation We report a special case of 45 years old female with malignant GCT of the bladder. Physical examination found a pelvic mass in the patient. Magnetic resonance imaging showed a huge mass between the posterior wall of the bladder, the cervix, and the anterior wall of the vagina. Pathological examination showed that the mass was 11×11×4.5cm in size, involved in the bladder's posterior wall. Under the microscope, the tumor cells were arranged in the shape of a nest or cord to infiltrate the bladder's wall. The tumor cells were pleomorphic, red-stained granular within the cytoplasm, with increased nuclear/cytoplasmic ratio, vacuolar nuclei, and obvious nucleoli. The tumor cells were showed obvious nuclear atypia, and the mitosis was more than 5/50HPF. Coagulative necrosis was widely showed within the tumor. Immunohistochemistry(IHC) showed that S-100, NSE, CD68, CR, α-AT, and TFE-3 were strongly positive, and the Ki-67 proliferation index was around 15%. The next-generation high throughput sequencing indicated that EP300 gene was missense mutated (c.457A>G) with 33% mutation abundance, and genes of DPYD(c.1627A>G),ERCC1( c.354T>C),NQO1(c.559C>T),TPMT(c.719A>G) and XRCC1(c.1196A>G) were polymorphic mutated. The patient died after three months of the second surgical treatment. Conclusions We report for the first time a primary bladder malignant GCM accompanied by mutations in special driving genes such as EP300. We also conducted a comprehensive literature review and an in-depth discussion.

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Granular Cell Tumor of the Larynx in a 5-Year-Old Child

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348948409300111 ◽

1984 ◽

Vol 93 (1) ◽

pp. 45-47 ◽

Cited By ~ 8

Author(s):

Ø. Gȧrrud ◽

H. H. Elverland ◽

L. Bostad ◽

I. W. S. Mair

Keyword(s):

Tumor Cells ◽

Electron Micrographs ◽

Granular Cell Tumor ◽

Anterior Commissure ◽

Granular Cell ◽

Cell Tumor ◽

Transmission Electron Micrographs ◽

Review Of The Literature ◽

Present Patient ◽

Transmission Electron

The youngest reported case of laryngeal granular cell tumor is presented together with transmission electron micrographs of the tumor cells. Whereas subglottic involvement is unusual in adults, a review of the literature reveals that three of the seven youngest patients (including the present patient) with laryngeal granular cell lesions had a subglottic tumor immediately below the anterior commissure.

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Bladder malignant granular cell tumor with EP300 gene mutation: a case report and literature review

10.21203/rs.3.rs-337088/v2 ◽

2021 ◽

Author(s):

Jian Huang ◽

Di Zhu ◽

Xinle Ren ◽

Bing Huang

Keyword(s):

Literature Review ◽

Tumor Cells ◽

Gene Mutation ◽

Granular Cell Tumor ◽

Granular Cell ◽

Posterior Wall ◽

Cell Tumor ◽

Proliferation Index ◽

Pathological Examination ◽

Malignant Granular Cell Tumor

Abstract Background Malignant granular cell tumor (GCT) is extremely rare. Malignant GCT with EP300 gene mutation in the bladder has not been reported in the literature. Case presentation We report a special case of 45 years old female with malignant GCT of the bladder. Physical examination found a pelvic mass in the patient. Magnetic resonance imaging showed a huge mass between the posterior wall of the bladder, the cervix, and the anterior wall of the vagina. Pathological examination showed that the mass was 11×11×4.5cm in size, involved in the bladder's posterior wall. Under the microscope, the tumor cells were arranged in the shape of a nest or cord to infiltrate the bladder's wall. The tumor cells were pleomorphic, red-stained granular within the cytoplasm, with increased nuclear/cytoplasmic ratio, vacuolar nuclei, and obvious nucleoli. The tumor cells were showed obvious nuclear atypia, and the mitosis was more than 5/50HPF. Coagulative necrosis was widely showed within the tumor. Immunohistochemistry(IHC) showed that S-100, NSE, CD68, CR, α-AT, and TFE-3 were strongly positive, and the Ki-67 proliferation index was around 15%. The next-generation high throughput sequencing indicated that EP300 gene was missense mutated (c.457A>G) with 33% mutation abundance, and genes of DPYD(c.1627A>G),ERCC1( c.354T>C),NQO1(c.559C>T),TPMT(c.719A>G) and XRCC1(c.1196A>G) were polymorphic mutated. The patient died after three months of the second surgical treatment. Conclusions We report for the first time a primary bladder malignant GCM accompanied by mutations in special driving genes such as EP300. We also conducted a comprehensive literature review and an in-depth discussion.

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