An Electron Microscopy Study of the Glycocalyx of Isolated Germinal Cells of the Rat

1975 ◽  
Vol 33 ◽  
pp. 596-597
Author(s):  
P. Hernández-Jáuregui ◽  
R. Trejo-Bayona ◽  
G. Delhumeau-Ongay
Keyword(s):  
Electron Microscopy ◽  
Mechanical Treatment ◽  
Plant Cells ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Surface Coat ◽  
Isolated Cells ◽  
Specific Permeability ◽  
Germinal Cells ◽  
Prophase Of Meiosis

The surface coat or glycocalyx of animal and plant cells plays an important role in cell properties such as antigenicity, specific permeability, and ATP ase activity. In the spermatozoa the glycocalyx may be of great importance in capacitation and fertilization phenomena. The glycocalyx is rich in fucose, mannose, galactose and sialic acid. These components are diminished during cell division. During spermatogenesis in mammals, spermatocytes represent a long prophase of meiosis. The purpose of this work was to demostrate the possible variations in the amount of glycocalyx in isolated germinal cells during spermatogenesis differentiation.Rat testes from 10, 23, and 38 days old, were finely scissored, in presence of Eagle essential medium, (mechanical treatment) Another similar group of isolated cells was treated with colagenase. (chemical treatment). Once separation of cells was completed, samples were treated for cytochemical electron microscopy using Alcian blue-lanthanum nitrate as previously described.

Factors influencing adhesive bonding at the bone/implant interface

1992 ◽  
Vol 50 (2) ◽  
pp. 1114-1115
Author(s):  
Julie A. Martini ◽  
Robert H. Doremus
Keyword(s):  
Electron Microscopy ◽  
Adhesive Bonding ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Bone Implant ◽  
Factors Influencing ◽  
Lr White ◽  
Transmission Electron ◽  
New Zealand White Rabbits ◽  
Scanning Electron

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.

Transmission Electron Microscopy study of Bi-based superconducting phases

1990 ◽  
Vol 48 (4) ◽  
pp. 50-51
Author(s):  
J.G. Wen ◽  
K.K. Fung
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Single Crystals ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Space Groups ◽  
Superconducting Phases ◽  
Ceramic Samples ◽  
Transmission Electron ◽  
Modulated Structures

Bi-based superconducting phases have been found to be members of a structural series represented by Bi2Sr2Can−1Cun−1On+4, n=1,2,3, and are referred to as 2201, 2212, 2223 phases. All these phases are incommensurate modulated structures. The super space groups are P2/b, NBbmb 2201, 2212 phases respectively. Pb-doped ceramic samples and single crystals and Y-doped single crystals have been studied by transmission electron microscopy.Modulated structures of all Bi-based superconducting phases are in b-c plane, therefore, it is the best way to determine modulated structure and c parameter in diffraction pattern. FIG. 1,2,3 show diffraction patterns of three kinds of modulations in Pb-doped ceramic samples. Energy dispersive X-ray analysis (EDAX) confirms the presence of Pb in the three modulated structures. Parameters c are 3 0.06, 38.29, 30.24Å, ie 2212, 2223, 2212 phases for FIG. 1,2,3 respectively. Their average space groups are all Bbmb.

Electron microscopy study of microtexture in Cu-Al-Ni shape memory alloys processed by powder metallurgy

2003 ◽  
Vol 112 ◽  
pp. 615-618 ◽  
Author(s):  
P. P. Rodriguez ◽  
J. San Jean ◽  
A. Iza-Mendia ◽  
V. Recarte ◽  
J. J. Pérez-Landazabal ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Powder Metallurgy ◽  
Shape Memory Alloys ◽  
Shape Memory ◽  
Microscopy Study ◽  
Electron Microscopy Study

On the morphology and taxonomy of Hannaea arcus (Bacillariophyta)

2008 ◽  
Vol 42 ◽  
pp. 14-22
Author(s):  
S. I. Genkal ◽  
V. G. Kharitonov
Keyword(s):  
Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Morphological Features ◽  
New Combination ◽  
Specific Rank ◽  
Ultraoligotrophic Lake ◽  
Emended Diagnosis

This electron microscopy study of the materials from ultraoligotrophic lake Elgygytgyn (Chukotka) revealed variability of morphological features in Hannaea arcus var. arcus and H. arcus var. recta. An emended diagnosis for H. arcus var. arcus is presented, and a new combination in specific rank is published: H. inaequidentata (Lagerstedt) Genkal et Kharitonov (= H. arcus var. recta).

To morphology, ecology and distribution of rare species Sellaphora hustedtii (Krasske) Lange-Bertalot et Werum (Bacillariophyta) from waterbodies of the Gydan Peninsula

2014 ◽  
Vol 48 ◽  
pp. 57-65 ◽  
Author(s):  
S. I. Genkal ◽  
M. I. Jarushina
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
River Basin ◽  
Rare Species ◽  
Western Siberia ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Diagnostic Features ◽  
Scanning Electron Microscopy Study ◽  
Scanning Electron

A rare species Sellaphora hustedtii, which is also new for the flora of Russia, has been found in waterbodies of the Messoyakha River basin (south-western part of the Gydan Peninsula, Western Siberia). The scanning electron microscopy study revealed variability of the main quantitative and qualitative diagnostic features of the species (length and width of the valve, number of striae and areolae in 10 μm, shape of the valve and structure of the raphe). New data on the ecology and distribution of the species allowed us to specify its diagnosis.

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