scholarly journals Usefulness of ribotyping in a molecular epidemiology study of shigellosis

1996 ◽  
Vol 116 (2) ◽  
pp. 127-135 ◽  
Author(s):  
M. C. Mendoza ◽  
M. C. Martín ◽  
M. A. González-Hevia
Keyword(s):  
Molecular Epidemiology ◽  
Genetic Heterogeneity ◽  
Reference Strain ◽  
Shigella Sonnei ◽  
The Other ◽  
Epidemiology Study ◽  
Sporadic Cases ◽  
Clonal Lines ◽  
Reference Strains ◽  
Discriminatory Index

SUMMARYRibotyping performed with six restriction endonucleases was used to study the molecular epidemiology of shigellosis in Asturias, Spain. The series includedShigella sonneifrom 34 sporadic cases, 3 outbreaks and 3 reference strains, andS. flexnerifrom 13 sporadic cases and 1 reference strain. TheS. sonneistrains were grouped into 5 ribotypes withSalI, 4 withHindIII andPvuII, 3 withBglII andEcoR I and 2 withHincII (Discriminatory Index (DI) between 0·54 and 0·14); theS. flexneriinto 5 ribotypes withSalI, Hinc II andHindIII, and 4 with the other enzymes (DI = 0·71–0·63). The combination of results for 2 or more enzymes facilitated an additional discrimination, the highest values inS. sonneiwere for the 6 enzymes (16 types, DI = 0·91) and inS. flexnerifor some combinations of 3 or more enzymes (7 types, DI = 0·81). Ribotypes with the 6 enzymes defined 16 clonal lines inS. sonneiand 7 inS. flexneri, which showed a different degree of genetic heterogeneity, and all the lines of each species falling into a different cluster. No line appeared as clearly endemic in the bowels of Asturian people.

scholarly journals Genetic Heterogeneity ofAlicyclobacillusStrains Revealed by RFLP Analysis ofvdcRegion andrpoBGene

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Agnieszka Dekowska ◽  
Jolanta Niezgoda ◽  
Barbara Sokołowska
Keyword(s):  
Cluster Analysis ◽  
16S Rdna ◽  
Genetic Heterogeneity ◽  
Reference Strain ◽  
Rflp Analysis ◽  
The Other ◽  
16S Rdna Gene ◽  
Restriction Patterns ◽  
Pcr Rflp ◽  
Reference Strains

PCR-RFLP targeting of the 16S rDNA andrpoBgenes, as well as thevdcregion, was applied to identify and differentiate between the spoilage and non-spoilageAlicyclobacillusspecies. Eight reference strains and 75 strains isolated from spoiled juices, juice concentrates, drinks, its intermediates, and fresh apples were subject to study. Hin6I restriction patterns of the 16S rDNA gene enabled distinguishing between all the species analyzed, while therpoBgene andvdcgene cluster analysis also revealed that there were two major types among theA. acidoterrestrisisolates, one similar to the reference strainA. acidoterrestrisDSM 2498, and the other similar to the reference strainA. acidoterrestrisATCC 49025. Heterogeneity was also observed among theA. acidocaldariusisolates. RFLP analysis of the 16S rDNA andrpoBgenes, as well asvdcregion, can be used successfully in the identification and research of intraspecies heterogeneity of theAlicyclobacillusspecies.

scholarly journals Epidemiology Study for Cancer Incidences among Patients from Baghdad Carrying Different Types of Cancer.

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Dawood Salim Edan ◽  
HamedH Khamees
Keyword(s):  
Breast Cancer ◽  
Colorectal Cancer ◽  
Bladder Cancer ◽  
Endometrial Cancer ◽  
Cancer Colorectal ◽  
Cancer Epidemiology ◽  
The Other ◽  
Epidemiology Study ◽  
Different Types ◽  
Prevalence Of Cancer

This study was carried out in Al-Yarmook hospital,laboratories department,Baghdad- Iraq; One hundred thirty three Iraqi patients have been recorded during period May 2014 until December 2014.The current study has demonstrated that five different types of the following cancers: Breast cancer,Skin cancer,colorectal cancer,Bladder cancer and endometrial cancer were enrolled in this study. Comparison among each type of cancer was regarded in age, sex and number. The majority results in cancer epidemiology for the present study were fluctuated between the skin and breast cancer,which showed 33.1% and this,represented the prevalence of cancer among all the other types of cancers. In case of other types,the result has showed 16.5 %,13.5% and 3.7 % for endometrial cancer,Bladder cancer and colorectal cancer,respectively.

scholarly journals Utility of mycobacterial interspersed repetitive unit typing for differentiating Mycobacterium tuberculosis isolates in Wuhan, China

2007 ◽  
Vol 56 (9) ◽  
pp. 1219-1223 ◽  
Author(s):  
Hui Han ◽  
Fang Wang ◽  
Yong Xiao ◽  
Yi Ren ◽  
Yanjie Chao ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Molecular Epidemiology ◽  
High Throughput ◽  
Discriminatory Power ◽  
Fast Method ◽  
Beijing Family ◽  
Wuhan City ◽  
Preliminary Screening ◽  
Discriminatory Index

Mycobacterial interspersed repetitive unit (MIRU) typing has been found to allow rapid, reliable, high-throughput genotyping of Mycobacterium tuberculosis, and may represent a feasible approach to study M. tuberculosis molecular epidemiology. To evaluate the use of MIRU typing in discriminating M. tuberculosis strains, isolates from 105 patients in Wuhan City, China, were genotyped by this method as compared to spoligotyping. MIRU typing identified 55 types that defined 21 clusters and 34 unique isolates. The discriminatory power was high [Hunter–Gaston discriminatory index (HGDI), 0.97]. Spoligotyping showed that 86 (81.9 %) of 105 isolates belonged to the Beijing family genotype. For Beijing family and non-Beijing strains, the discriminatory power of MIRU was high (HGDI, 0.95 and 0.98, respectively). Among the alleles of the MIRU loci for the Beijing family, only locus 26 was highly discriminative, but for non-Beijing strains, loci 10, 16 and 26 were highly discriminative. MIRU typing is a simple and fast method which may be used for preliminary screening of M. tuberculosis isolates in China.

Oxacillin- and Quinolone-Resistant Staphylococcus aureus in Sao Paulo, Brazil: A Multicenter Molecular Epidemiology Study

1993 ◽  
Vol 14 (5) ◽  
pp. 260-264 ◽  
Author(s):  
Helio S. Sader ◽  
Antonio C. Pignatari ◽  
Richard J. Hollis ◽  
Ivani Leme ◽  
Ronald N. Jones
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Epidemiology ◽  
Sao Paulo ◽  
São Paulo ◽  
Epidemiology Study

scholarly journals Bacillus thuringiensis isolates entomopathogenic for Culex quinquefasciatus (Diptera: Culicidae) and Anticarsia gemmatalis (Lepidoptera: Noctuidae)

2010 ◽  
Vol 70 (4) ◽  
pp. 1039-1046 ◽  
Author(s):  
V. Gobatto ◽  
SG. Giani ◽  
M. Camassola ◽  
AJP. Dillon ◽  
A. Specht ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Culex Quinquefasciatus ◽  
Reference Strain ◽  
Probit Analysis ◽  
Anticarsia Gemmatalis ◽  
Sds Page ◽  
Pathogenicity Tests ◽  
Lepidoptera Noctuidae ◽  
Reference Strains ◽  
Cry1 Gene

Samples of the Bacillus thuringiensis (Bt) were collected from soil and insects. Eight isolates were selected from rural soil, 15 from urban soil and 11 from insects. These were evaluated for entomopathogenicity against larvae of Anticarsia gemmatalis and Culex quinquefasciatus. The pathogenicity tests showed that a higher percentage of isolates were active against A. gemmatalis (60%) compared to C. quinquefasciatus (31%). Probit analysis (LC50) indicated that against A. gemmatalis four of the isolates presented values similar to the reference strain against A. gemmatalis, while against C. quinquefasciatus one isolate showed an LC50 similar to the reference strain (IPS-82). SDS-PAGE characterisation of two isolates showed a 27 kDa protein fraction related to the Bt subspecies israelensis cytolytic toxin (cyt) gene. One 130 kDa protein, possibly related to the Bt crystal inclusions (cry1) gene, was identified in the other two isolates, which were more toxic for lepidoptera; another isolate presented a protein of 100 kDa. Some new local Bt isolates had similar LC50 probit values to the reference strains.

A molecular epidemiology study reveals the presence of identical genotypes on grapevines and ground cover weeds and the existence of separate genetic groups in a Botrytis cinerea population

2020 ◽  
Vol 69 (9) ◽  
pp. 1695-1707
Author(s):  
Silvia L. Toffolatti ◽  
Giuliana Maddalena ◽  
Demetrio Marcianò ◽  
Alessandro Passera ◽  
Fabio Quaglino
Keyword(s):  
Molecular Epidemiology ◽  
Botrytis Cinerea ◽  
Ground Cover ◽  
Epidemiology Study ◽  
Genetic Groups
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