Age-specific long-term course of IgG antibodies to pertussis toxin after symptomatic infection with Bordetella pertussis

AbstractPertussis is a vaccine-preventable disease and antibodies formed are known to decline with time. The aim of this study was to measure Bordetella pertussis/toxin immunoglobulin G (IgG) antibodies in different age groups of Nigerian children and determine the age at which booster dose may be required. A total of 422 children, aged 6 to 60 months, were tested for the presence of B. pertussis/toxin IgG antibodies by ELISA (enzyme-linked immune sorbent assay). The highest positivity rate was in the 6 to 11 months of age group, while the highest negativity rate was in the age group of 24 to 35 months. We conclude that B. Pertussis/toxin IgG antibodies response is weak in Nigerian children after three doses of DPT (diphtheria, pertussis, and tetanus)/pentavalent vaccination, and there is a rapid decline of antibody levels between 12 and 35 months. We recommend that booster vaccination should be given at 12 to 15 months of age.

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Pertussis surveillance by small serosurveys of blood donors

Epidemiology and Infection ◽

10.1017/s0950268818001851 ◽

2018 ◽

Vol 146 (14) ◽

pp. 1807-1810 ◽

Cited By ~ 1

Author(s):

N. Kennerknecht ◽

M. Riffelmann ◽

U. Voelker ◽

C. H. Wirsing von König

Keyword(s):

Pertussis Toxin ◽

Bordetella Pertussis ◽

Immunoglobulin G ◽

Blood Donors ◽

Igg Antibodies ◽

Cheap Method ◽

Immunosorbent Assays ◽

Observation Period

AbstractSerosurveys have established data about the distribution of immunoglobulin G (IgG)-antibodies to pertussis toxin (PT) in various populations. We tried to detect whether small serosurveys in blood donors could serve as a simple and inexpensive means to collect information about the circulation ofBordetella pertussis. We screened every donation in 307 adult blood donors aged 19–69 years for IgG-anti-PT by standardised enzyme-linked immunosorbent assays (ELISA), and the donors were followed between 2014 and 2016 for a total of 426 person-years. When we used a vertical survey with cut-offs of 100, 62.5 and 40 IU/ml, respectively, as an indicator for recent contacts withB. pertussis, nine (2.9%), 22 (7.2%) and 54 (17.6%) of donors had IgG-anti-PT titres above the respective levels. During the horizontal observation period of 426 person years, six significant increases and two conversions were found, which lead to an estimate of 1878 contacts/100.000 person-years (1.9% per year). Median and mean IgG-anti-PT concentrations remained relatively stable from year to year during the observation period. Our findings show that small serosurveys of blood donors offer a simple and cheap method for the surveillance ofB. pertussis.

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LONG-TERM PRESERVATION OF MEASLES AND RUBELLA SPECIFIC-IGG ANTIBODIES IN CHILDREN WITH JUVENILE SPONDYLARTHRITIS ON ANTI-TNFΑ TREATMENT- A PROSPECTIVE CONTROLLED STUDY

10.26226/morressier.5ad774e0d462b80296ca6c3d ◽

2018 ◽

Author(s):

John Kopsidas

Keyword(s):

Controlled Study ◽

Igg Antibodies ◽

Specific Igg ◽

Prospective Controlled Study ◽

Long Term Preservation ◽

Specific Igg Antibodies

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Importance of Holotoxin Assembly in Ptl-Mediated Secretion of Pertussis Toxin from Bordetella pertussis

Infection and Immunity ◽

10.1128/iai.68.7.4049-4054.2000 ◽

2000 ◽

Vol 68 (7) ◽

pp. 4049-4054 ◽

Cited By ~ 39

Author(s):

Karen M. Farizo ◽

Theresa Huang ◽

Drusilla L. Burns

Keyword(s):

Pertussis Toxin ◽

Bordetella Pertussis ◽

Structural Components ◽

Type Iv ◽

System A

ABSTRACT We examined the structural components of pertussis toxin that are required for efficient export from Bordetella pertussis via the Ptl system, a member of the type IV family of macromolecular transporters. First, we constructed a strain of B. pertussis that contains a functional Ptl system but does not produce pertussis toxin. Plasmids which express either the S1 subunit or the B oligomer were then introduced into this strain. We found that the B oligomer of the toxin is not secreted in the absence of the S1 subunit. Conversely, the S1 subunit is also not secreted by a Ptl-mediated mechanism in the absence of the B oligomer. Thus, an assembled holotoxin is required for Ptl-mediated export of pertussis toxin from B. pertussis.

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Rapid detection of the recently emerged Bordetella pertussis strains with the ptxP3 pertussis toxin promoter allele by real-time PCR

Clinical Microbiology and Infection ◽

10.1111/j.1469-0691.2012.04000.x ◽

2012 ◽

Vol 18 (10) ◽

pp. E377-E379 ◽

Cited By ~ 18

Author(s):

T. Kallonen ◽

J. Mertsola ◽

F.R. Mooi ◽

Q. He

Keyword(s):

Real Time ◽

Pertussis Toxin ◽

Bordetella Pertussis ◽

Real Time Pcr ◽

Rapid Detection ◽

Promoter Allele

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Diagnostic performance of commercial serological assays measuring Bordetella pertussis IgG antibodies

Diagnostic Microbiology and Infectious Disease ◽

10.1016/j.diagmicrobio.2017.11.006 ◽

2018 ◽

Vol 90 (3) ◽

pp. 157-162 ◽

Cited By ~ 3

Author(s):

Giorgio Fedele ◽

Pasqualina Leone ◽

Stefania Bellino ◽

Ilaria Schiavoni ◽

Claudia Pavia ◽

...

Keyword(s):

Bordetella Pertussis ◽

Diagnostic Performance ◽

Igg Antibodies

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Declining Levels of Neutralizing Antibodies Against SARS-CoV-2 in Convalescent COVID-19 Patients One Year Post Symptom Onset

Frontiers in Immunology ◽

10.3389/fimmu.2021.708523 ◽

2021 ◽

Vol 12 ◽

Author(s):

Tiandan Xiang ◽

Boyun Liang ◽

Yaohui Fang ◽

Sihong Lu ◽

Sumeng Li ◽

...

Keyword(s):

Symptom Onset ◽

Neutralizing Antibodies ◽

Natural Infection ◽

Protein S ◽

Igg Antibodies ◽

Neutralizing Activity ◽

Specific Igg ◽

One Year ◽

Kinetics Of

Major advances have been made in understanding the dynamics of humoral immunity briefly after the acute coronavirus disease 2019 (COVID-19). However, knowledge concerning long-term kinetics of antibody responses in convalescent patients is limited. During a one-year period post symptom onset, we longitudinally collected 162 samples from 76 patients and quantified IgM and IgG antibodies recognizing the nucleocapsid (N) protein or the receptor binding domain (RBD) of the spike protein (S). After one year, approximately 90% of recovered patients still had detectable SARS-CoV-2-specific IgG antibodies recognizing N and RBD-S. Intriguingly, neutralizing activity was only detectable in ~43% of patients. When neutralization tests against the E484K-mutated variant of concern (VOC) B.1.351 (initially identified in South Africa) were performed among patients who neutralize the original virus, the capacity to neutralize was even further diminished to 22.6% of donors. Despite declining N- and S-specific IgG titers, a considerable fraction of recovered patients had detectable neutralizing activity one year after infection. However, neutralizing capacities, in particular against an E484K-mutated VOC were only detectable in a minority of patients one year after symptomatic COVID-19. Our findings shed light on the kinetics of long-term immune responses after natural SARS-CoV-2 infection and argue for vaccinations of individuals who experienced a natural infection to protect against emerging VOC.

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