scholarly journals Greek measles epidemic strain, 2005–2006

2006 ◽  
Vol 135 (4) ◽  
pp. 570-573 ◽  
Author(s):  
G. GIOULA ◽  
A. PAPA ◽  
M. EXINDARI ◽  
A. MELIDOU ◽  
D. CHATZIDIMITRIOU ◽  
...  
Keyword(s):  
Phylogenetic Tree ◽  
Vaccine Strain ◽  
Virus Strain ◽  
Measles Virus ◽  
Molecular Study ◽  
N Gene ◽  
Epidemic Strain ◽  
Rt Pcr ◽  
Measles Outbreak ◽  
Genotype A

SUMMARYThe purpose of this work was the molecular study of the virus strain that caused the last measles outbreak in Greece. Twenty-four saliva specimens were obtained from selected patients serologically confirmed as measles cases between December 2005 and March 2006. Measles virus (MV) detection was performed by a nested RT–PCR. The 447-bp segment of the N gene of these MV strains was used for genotyping. The N gene sequences of the Greek MV strains were identical to each other, therefore a phylogenetic tree was constructed using one representative MV (ThesGRE/06). Our data show that the MV strain which caused the 2005–2006 outbreak in Greece belongs to genotype D6, and differs by 0·68% from the New Jersey D6 strain and by 5·5% from the MV vaccine strain Edmonston B (U03656) belonging to genotype A.

P1444 Evaluation of a real-time RT-PCR assay for the diagnosis of measles virus infections during a measles outbreak in Greece

2007 ◽  
Vol 29 ◽  
pp. S403
Author(s):  
S. Kokotas ◽  
M. Giannaki ◽  
E. Horefti ◽  
D. Sgouras ◽  
M. Logothetis ◽  
...  
Keyword(s):  
Real Time ◽  
Measles Virus ◽  
Virus Infections ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Measles Outbreak

scholarly journals Rapid Identification of Measles Virus Vaccine Genotype by Real-Time PCR

2016 ◽  
Vol 55 (3) ◽  
pp. 735-743 ◽  
Author(s):  
Felicia Roy ◽  
Lillian Mendoza ◽  
Joanne Hiebert ◽  
Rebecca J. McNall ◽  
Bettina Bankamp ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Measles Virus ◽  
Rapid Identification ◽  
Rt Pcr ◽  
Outbreak Response ◽  
Reverse Transcription Pcr ◽  
Pcr Method ◽  
Measles Outbreaks ◽  
Genotype A

ABSTRACT During measles outbreaks, it is important to be able to rapidly distinguish between measles cases and vaccine reactions to avoid unnecessary outbreak response measures such as case isolation and contact investigations. We have developed a real-time reverse transcription-PCR (RT-PCR) method specific for genotype A measles virus (MeV) (MeVA RT-quantitative PCR [RT-qPCR]) that can identify measles vaccine strains rapidly, with high throughput, and without the need for sequencing to determine the genotype. We have evaluated the method independently in three measles reference laboratories using two platforms, the Roche LightCycler 480 system and the Applied Biosystems (ABI) 7500 real-time PCR system. In comparison to the standard real-time RT-PCR method, the MeVA RT-qPCR showed 99.5% specificity for genotype A and 94% sensitivity for both platforms. The new assay was able to detect RNA from five currently used vaccine strains, AIK-C, CAM-70, Edmonston-Zagreb, Moraten, and Shanghai-191. The MeVA RT-qPCR assay has been used successfully for measles surveillance in reference laboratories, and it could be readily deployed to national and subnational laboratories on a wide scale.

scholarly journals Spotlight on measles 2010: Excretion of vaccine strain measles virus in urine and pharyngeal secretions of a child with vaccine associated febrile rash illness, Croatia, March 2010

2010 ◽  
Vol 15 (35) ◽  
Author(s):  
B Kaic ◽  
I Gjenero-Margan ◽  
B Aleraj ◽  
T Vilibić-Čavlek ◽  
M Santak ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Measles Virus ◽  
Measles Vaccine ◽  
Primary Immunisation ◽  
Urine Specimens ◽  
Genotype A

We describe excretion of measles vaccine strain Schwarz in a child who developed a febrile rash illness eight days after primary immunisation against measles, mumps and rubella. Throat swabs and urine specimens were collected on the fifth and sixth day of illness, respectively. Genotyping demonstrated measles vaccine strain Schwarz (genotype A). If measles and rubella were not under enhanced surveillance in Croatia, the case would have been either misreported as rubella or not recognised at all.

scholarly journals Molecular genetic analysis of the strain Leningrad-16 used for the production of measles vaccine

2020 ◽  
Vol 97 (2) ◽  
pp. 182-189
Author(s):  
Georgy M. Ignatyev ◽  
Alena V. Atrasheuskaya ◽  
Lidiya L. Sukhanova ◽  
Elena S. Sidorenko ◽  
Nina A. Netesova
Keyword(s):  
Vaccine Strain ◽  
Measles Virus ◽  
Restriction Analysis ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Rt Pcr ◽  
Virus Genotype ◽  
Molecular Genetic Study ◽  
Production Stages ◽  
Pcr Method

Aim. To study the genetic stability of the measles virus strain Leningrad-16 (L-16) used for the production of vaccine at JSC NPO Mikrogen.Materials and methods. A series of production and sowing strains of L-16 (JSC NPO Mikrogen), ready-made series of measles vaccines from various manufacturers, and the strain of measles virus genotype D6 were studied. Molecular genetic study of the strains was performed using RT-PCR followed by restriction analysis and sequencing.Results. The complete genome sequences of the production and sowing strains of L-16 that are used for vaccine production were obtained. The sequence of the vaccine strain was deposited in GenBank. Strain L-16 was confirmed to be genetically stable. The obtained data demonstrated the possibility of using the RT-PCR method with subsequent restriction analysis to confirm the authenticity of the vaccine strain L-16 in finished mono and three component vaccines.Conclusion. The results of the study suggest the applicability of the molecular genetic methods to confirm the authenticity of the studied strains not only at the production stages, but also in the finished series of vaccines.

scholarly journals Development of TaqMan-based real-time RT-PCR assay based on N gene for the quantitative detection of feline morbillivirus

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Siti Tasnim Makhtar ◽  
Sheau Wei Tan ◽  
Nur Amalina Nasruddin ◽  
Nor Azlina Abdul Aziz ◽  
Abdul Rahman Omar ◽  
...  
Keyword(s):  
Real Time ◽  
Measles Virus ◽  
Clinical Sample ◽  
N Gene ◽  
Clinical Samples ◽  
Quantitative Detection ◽  
High Morbidity ◽  
Canine Distemper ◽  
Rt Pcr ◽  
Pcr Assay

Abstract Background Morbilliviruses are categorized under the family of Paramyxoviridae and have been associated with severe diseases, such as Peste des petits ruminants, canine distemper and measles with evidence of high morbidity and/or could cause major economic loss in production of livestock animals, such as goats and sheep. Feline morbillivirus (FeMV) is one of the members of Morbilliviruses that has been speculated to cause chronic kidney disease in cats even though a definite relationship is still unclear. To date, FeMV has been detected in several continents, such as Asia (Japan, China, Thailand, Malaysia), Europe (Italy, German, Turkey), Africa (South Africa), and South and North America (Brazil, Unites States). This study aims to develop a TaqMan real-time RT-PCR (qRT-PCR) assay targeting the N gene of FeMV in clinical samples to detect early phase of FeMV infection. Results A specific assay was developed, since no amplification was observed in viral strains from the same family of Paramyxoviridae, such as canine distemper virus (CDV), Newcastle disease virus (NDV), and measles virus (MeV), and other feline viruses, such as feline coronavirus (FCoV) and feline leukemia virus (FeLV). The lower detection limit of the assay was 1.74 × 104 copies/μL with Cq value of 34.32 ± 0.5 based on the cRNA copy number. The coefficient of variations (CV) values calculated for both intra- and inter-assay were low, ranging from 0.34–0.53% and 1.38–2.03%, respectively. In addition, the clinical sample evaluation using this assay showed a higher detection rate, with 25 (35.2%) clinical samples being FeMV-positive compared to 11 (15.5%) using conventional RT-PCR, proving a more sensitive assay compared to the conventional RT-PCR. Conclusions The TaqMan-based real-time RT-PCR assay targeting the N gene described in this study is more sensitive, specific, rapid, and reproducible compared to the conventional RT-PCR assay targeting the N gene, which could be used to detect early infection in cats.

scholarly journals Development of Taqman-Based Real-Time RT-PCR Assay Based on N Gene for The Quantitative Detection of Feline Morbillivirus

2020 ◽  
Author(s):  
Siti Tasnim Makhtar ◽  
Sheau Wei Tan ◽  
Nur Amalina Nasruddin ◽  
Nor Azlina Abdul Aziz ◽  
Abdul Rahman Omar ◽  
...  
Keyword(s):  
Real Time ◽  
Measles Virus ◽  
Clinical Sample ◽  
Feline Leukemia Virus ◽  
N Gene ◽  
Clinical Samples ◽  
Quantitative Detection ◽  
Canine Distemper ◽  
Rt Pcr ◽  
Pcr Assay

Abstract Background: Feline morbillivirus (FeMV) is a member of genus Morbillivirus which has been associated with the chronic kidney disease in cats even though a definite relationship is still unclear. Morbilliviruses are associated with severe diseases such as Peste des petits ruminants, canine distemper and measles. FeMV has been detected in many countries including Malaysia. This study aims to develop a Taqman real-time RT-PCR (qRT-PCR) assay targeting the N gene of FeMV in clinical samples to detect early phase of FeMV infection.Results: A specific assay was developed since no amplification was observed in viral strains from the same Paramyxoviridae family, such as canine distemper virus (CDV), Newcastle disease virus (NDV) and measles virus (MeV), and other feline viruses, such as feline coronavirus (FCoV) and feline leukemia virus (FeLV). The lower detection limit of the assay was 1.74 x 104 copies/L with Cq value of 34.32 0.5 based on the cRNA copy number. The coefficient of variations (CV) values calculated for both intra- and inter-assay were low, ranging from 0.34% - 0.53% and 1.38% - 2.03%, respectively. Besides that, the clinical sample evaluation using this assay showed a higher detection rate, with 26 (37%) clinical samples being FeMV-positive compared to 11 (15.5%) using conventional RT-PCR, proving a more sensitive assay compared to the conventional RT-PCR.Conclusions: The Taqman-based real-time RT PCR assay targeting the N gene described here is more sensitive, specific, rapid and reproducible compared to the conventional RT-PCR assay targeting the N gene and it could be used to detect early infection in cats.

scholarly journals Performance of Unobserved Self-Collected Nasal Swabs for Detection of SARS-CoV-2 by RT-PCR Utilizing a Remote Specimen Collection Strategy

2021 ◽  
Author(s):  
Ron M Kagan ◽  
Amy A Rogers ◽  
Gwynngelle A Borillo ◽  
Nigel J Clarke ◽  
Elizabeth M Marlowe
Keyword(s):  
Return To Work ◽  
Screening Program ◽  
False Negative ◽  
N Gene ◽  
Rt Pcr ◽  
Pooled Testing ◽  
Specimen Collection ◽  
Asymptomatic Patients ◽  
Nasal Swabs ◽  
The Impact

Abstract Background The use of a remote specimen collection strategy employing a kit designed for unobserved self-collection for SARS-CoV-2 RT-PCR can decrease the use of PPE and exposure risk. To assess the impact of unobserved specimen self-collection on test performance, we examined results from a SARS-CoV-2 qualitative RT-PCR test for self-collected specimens from participants in a return-to-work screening program and assessed the impact of a pooled testing strategy in this cohort. Methods Self-collected anterior nasal swabs from employee return to work programs were tested using the Quest Diagnostics SARS-CoV-2 RT-PCR EUA. The Ct values for the N1 and N3 N-gene targets and a human RNase P (RP) gene control target were tabulated. For comparison, we utilized Ct values from a cohort of HCP-collected specimens from patients with and without COVID-19 symptoms. Results Among 47,923 participants, 1.8% were positive. RP failed to amplify for 13/115,435 (0.011%) specimens. The median (IQR) Cts were 32.7 (25.0-35.7) for N1 and 31.3 (23.8-34.2) for N3. Median Ct values in the self-collected cohort were significantly higher than those of symptomatic, but not asymptomatic patients. Based on Ct values, pooled testing with 4 specimens would have yielded inconclusive results in 67/1,268 (5.2%) specimens but only a single false-negative result. Conclusions Unobserved self-collection of nasal swabs provides adequate sampling for SARS-CoV-2 RT-PCR testing. These findings alleviate concerns of increased false negatives in this context. Specimen pooling could be used for this population as the likelihood of false negative results is very low due when using a sensitive, dual-target methodology.

scholarly journals Molecular epidemiology of Crimean-Congo haemorrhagic fever virus in India

2016 ◽  
Vol 144 (16) ◽  
pp. 3422-3425 ◽  
Author(s):  
P. SINGH ◽  
M. CHHABRA ◽  
P. SHARMA ◽  
R. JAISWAL ◽  
G. SINGH ◽  
...  
Keyword(s):  
Eastern Europe ◽  
N Gene ◽  
Western India ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Study Region ◽  
Phylogenetic Relatedness ◽  
Cchf Virus ◽  
Crimean Congo Haemorrhagic Fever ◽  
First Time

SUMMARYCrimean-Congo haemorrhagic fever (CCHF) is an emerging zoonotic disease in India which is prevalent in neighbouring countries. CCHF virus (CCHFV) is a widespread tick-borne virus which is endemic in Africa, Asia, Eastern Europe and the Middle East. In the present study, samples of clinically suspected human cases from different areas of northern-western India were tested for the presence of CCHFV by RT–PCR through amplification of nucleocapsid (N) gene of CCHFV. Positive samples were sequenced to reveal the prevailing CCHFV genotype(s) and phylogenetic relatedness. A phylogenetic tree revealed the emergence of diverse strains in the study region showing maximum identity with the Pakistan, Afghanistan and Iran strains, which was different from earlier reported Indian strains. Our findings reveal for the first time the emergence of the Asia 1 group in India; while earlier reported CCHFV strains belong to the Asia 2 group.

Sign in / Sign up

Export Citation Format

Share Document