scholarly journals Experimental infection of ostriches with Crimean–Congo haemorrhagic fever virus

1998 ◽  
Vol 121 (2) ◽  
pp. 427-432 ◽  
Author(s):  
R. SWANEPOEL ◽  
P. A. LEMAN ◽  
F. J. BURT ◽  
J. JARDINE ◽  
D. J. VERWOERD ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Maximum Intensity ◽  
Post Inoculation ◽  
Haemorrhagic Fever ◽  
Infective Virus ◽  
Chain Reaction ◽  
Viral Nucleic Acid ◽  
Liver And Kidney ◽  
Crimean Congo Haemorrhagic Fever ◽  
Polymerase Chain

Following the occurrence of an outbreak of Crimean–Congo haemorrhagic fever (CCHF) among workers at an ostrich abattoir in South Africa in 1996, 9 susceptible young ostriches were infected subcutaneously with the virus in order to study the nature of the infection which they undergo. The ostriches developed viraemia which was demonstrable on days 1–4 following infection, with a maximum intensity of 4·0 log10 mouse intracerebral LD50/ml being recorded on day 2 in 1 of the birds. Virus was detectable in visceral organs such as spleen, liver and kidney up to day 5 post-inoculation, 1 day after it could no longer be found in blood. No infective virus was detected in samples of muscle, but viral nucleic acid was detected by reverse transcription-polymerase chain reaction in muscle from a bird sacrificed on day 3 following infection. It was concluded that the occurrence of infection in ostriches at abattoirs could be prevented by keeping the birds free of ticks for 14 days before slaughter.

Nosocomial outbreak of Crimean-Congo haemorrhagic fever

2010 ◽  
Vol 139 (6) ◽  
pp. 862-866 ◽  
Author(s):  
H. R. NADERI ◽  
M. R. SARVGHAD ◽  
A. BOJDY ◽  
M. R. HADIZADEH ◽  
R. SADEGHI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Direct Contact ◽  
Healthcare Workers ◽  
Index Case ◽  
Haemorrhagic Fever ◽  
Nosocomial Outbreak ◽  
Chain Reaction ◽  
Viral Haemorrhagic Fever ◽  
Crimean Congo Haemorrhagic Fever ◽  
Polymerase Chain

SUMMARYWe report a nosocomial outbreak of Crimean-Congo haemorrhagic fever (CCHF) that affected six patients in June 2009 in Ghaem Hospital, Mashhad, Iran, apparently related to one index case. The last four cases were healthcare workers. Infection was spread by percutaneous exposure to two cases, and probably by direct contact with blood, clothes and sheets, to three others. The diagnosis in the two fatal cases was not confirmed virologically. The diagnosis in four cases who survived was confirmed by specific reverse transcription polymerase chain reaction. The patients were treated with ribavirin. In endemic areas, every patient presenting with a febrile haemorrhagic syndrome should be considered to have a viral haemorrhagic fever until proven otherwise. Patients who meet the criteria for probable CCHF should be admitted to hospital and treated with ribavirin. Appropriate isolation precautions should be immediately initiated.

scholarly journals Pet rat harbouring Seoul hantavirus in Sweden, June 2013

2013 ◽  
Vol 18 (27) ◽  
Author(s):  
Å Lundkvist ◽  
J Verner-Carlsson ◽  
A Plyusnina ◽  
L Forslund ◽  
R Feinstein ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lung Tissue ◽  
Reverse Transcription ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Specific Antibodies ◽  
Polymerase Chain ◽  
Focus Reduction

We report the first detection of Seoul hantavirus (SEOV) in a pet rat in Sweden. SEOV-specific antibodies were detected in the pet rat blood by focus reduction neutralising test (FRNT), and SEOV RNA in lung tissue was confirmed by reverse transcription-nested polymerase chain reaction (RT-PCR) followed by sequencing. The discovery follows the recent reports of SEOV infected pet rats, as well as associated human cases of severe haemorrhagic fever with renal syndrome (HFRS), in England and Wales.

scholarly journals Detection of Bovine Leukemia Virus by in Situ Polymerase Chain Reaction in Tissues from a Heifer Diagnosed with Sporadic Thymic Lymphosarcoma

2005 ◽  
Vol 17 (2) ◽  
pp. 190-194 ◽  
Author(s):  
Robert B. Duncan ◽  
William K. Scarratt ◽  
Gertrude C. Buehring
Keyword(s):  
Polymerase Chain Reaction ◽  
Epithelial Cells ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Sudden Onset ◽  
Chain Reaction ◽  
Liver And Kidney ◽  
Cellular Tropism ◽  
Polymerase Chain

An 18-month-old bovine heifer was presented for clinical evaluation after a sudden onset of ventral edema. Clinical and pathological evaluations were consistent with thymic lymphosarcoma, a sporadic form of lymphosarcoma in cattle, which is not generally considered to be associated with bovine leukemia virus (BLV). This heifer was seropositive for BLV at 6 and 18 months of age. Tissues obtained at necropsy were evaluated using in situ polymerase chain reaction. The BLV proviral DNA was detected in lymphocytes of the thymus as well as in epithelial cells of the liver and kidney. This report presents evidence that thymic lymphosarcomas can be associated with BLV infection and that BLV may have a broader cellular tropism than was supposed previously.

Early and simultaneous detection ofNosema bombycis(Microsporidia: Nosematidae), nucleopolyhedrovirus (Baculoviridae), and densovirus (Parvoviridae) by multiplex real-time polymerase chain reaction inBombyx mori(Lepidoptera: Bombycidae)

2017 ◽  
Vol 149 (2) ◽  
pp. 265-275
Author(s):  
Shan Wu ◽  
Yong-Qiang He ◽  
Xing-Meng Lu ◽  
Xiao-Feng Zhang ◽  
Jiang-Bing Shuai ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Early Detection ◽  
Bombyx Mori ◽  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Post Inoculation ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain

AbstractAn effective multiplex real-time polymerase chain reaction (PCR) assay for the simultaneous detection of three major pathogens,Nosema bombycisNägeli (Microsporidia: Nosematidae),Bombyx morinucleopolyhedrovirus (Baculoviridae: genusAlphabaculovirus) (NPV), andBombyx moridensovirus (Parvoviridae: genusIteravirus) (DNV), in silkworms (Bombyx mori(Linnaeus); Lepidoptera: Bombycidae) was developed in this study. Polymerase chain reaction and real-time PCR tests and basic local alignment search tool searches revealed that the primers and probes used in this study had high specificities for their target species. The ability of each primer/probe set to detect pure pathogen DNA was determined using a plasmid dilution panel, in which under optimal conditions the multiplex real-time PCR assay showed high efficiency in the detection of three mixed target plasmids with a detection limit of 8.5×103copies forN. bombycisandBombyx moriNPV (BmNPV) and 8.5×104copies forBombyx moriDNV (BmDNV). When the ability to detect these three pathogens was examined in artificially inoculated silkworms, our method presented a number of advantages over traditional microscopy, including specificity, sensitivity, and high-throughput capabilities. Under the optimal volume ratio for the three primer/probe sets (3:2:2=N. bombycis:BmNPV:BmDNV), the multiplex real-time PCR assay showed early detection of BmNPV and BmDNV by day 1 post inoculation using DNA templates of the three pathogens in various combinations from individually infected silkworms; the early detection ofN. bombyciswas possible by day 3 post inoculation using the DNA isolated from the midgut ofN. bombycis-infected silkworms.

scholarly journals Pet rats as a source of hantavirus in England and Wales, 2013

2013 ◽  
Vol 18 (9) ◽  
Author(s):  
L J Jameson ◽  
S K Taori ◽  
B Atkinson ◽  
P Levick ◽  
C A Featherstone ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
United Kingdom ◽  
Reverse Transcription ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Chain Reaction ◽  
England And Wales ◽  
Wild Rats ◽  
Polymerase Chain

We report the detection of a strain of Seoul hantavirus (SEOV) in pet rats in England and Wales. The discovery followed an investigation of a case of haemorrhagic fever with renal syndrome in Wales. Hantavirus RNA was detected via real-time reverse transcription-polymerase chain reaction (RT-PCR) and classic RT-PCR in pet rats belonging to the patient. Sequencing and phylogenetic analysis confirmed the virus to be a SEOV that is similar, but not identical, to a previously reported United Kingdom strain from wild rats.

scholarly journals Possible re-infection of SARS-CoV-2 complicated by dengue virus co-infection: report of a rare case from Bangladesh

2020 ◽  
pp. 105-106
Author(s):  
Miah Wahiduzzaman ◽  
Muhammad Abdur Rahim
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Dengue Virus ◽  
Rare Case ◽  
Virus Disease ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Mild Form ◽  
Chain Reaction ◽  
Polymerase Chain

Re-infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and co-infection by dengue virus and SARS-CoV-2 are possible. We report a case of dengue haemorrhagic fever, occurring in a young Bangladeshi man, who concurrently tested positive for SARS-CoV-2 infection by reverse transcriptase polymerase chain reaction (RT-PCR). Four months previously, he suffered a mild form of corona virus disease 2019 (COVID-19). This case is reported to make the physicians aware that, co-infections are possible in this COVID-19 pandemic, specially in dengue endemic regions and countries like Bangladesh. Birdem Med J 2020; 10, COVID Supplement: 105-106

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