The effect of storage time and number of spermatozoa per insemination dose on semen characteristics and fertilizing capacity of boar semen diluted with Beltsville Thaw Solution (BTS) extender

AbstractSemen collection from three boars was performed twice a week, and doses (100 ml each) of semen diluted with Beltsville Thaw Solution (BTS) extender were prepared containing 1 × 109, 3 × 109 and 5 × 109 spermatozoa. Diluted semen was then stored at 17°C for a maximum of 120 h. Percentage of motile spermatozoa (PMS) and type of spermatozoa motility (TSM) were determined by microscopic examinations performed at 24-h intervals, from zero time (beginning of storage at 17°C) up to 120 h of storage, and a sperm motility index (SMI) calculated. Sows (no. = 360) were divided into nine experimental groups (40 sows per group) and a combination of number of spermatozoa per dose and storage time was used for artificial insemination in each group. All the animals were inseminated twice at 12 and 36 h after detection of standing oestrus by a teaser boar. Return and farrowing data including litter size were recorded. PMS, TSM and SMI decreased significantly (P < 0·01) from 48 h up to 120 h of storage, this being more marked in semen doses of 5 × 109 spermatozoa. When semen stored for up to 24 h was used, no significant differences (P > 0·05) regarding return rates and farrowing rates were observed among the three groups inseminated with semen containing different numbers of spermatozoa per dose. Inseminations with semen doses of 1 × 109 and 3 × 109 spermatozoa stored for 48 to 72 h have significantly lower return rates (P < 0·05) and significantly higher farrowing rates (P < 0·05) than with doses of 5 × 109 spermatozoa. Inseminations with semen stored for more than 72 h gave relatively higher return rates and lower farrowing rates. Semen storage time and number of spermatozoa per dose appeared to reduce litter size only where semen doses of 5 X109 spermatozoa were inseminated after storage for more than 48 h.

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Effect of commercial long-term extenders on metabolic activity and membrane integrity of boar spermatozoa stored at 17°C

Polish Journal of Veterinary Sciences ◽

10.2478/pjvs-2013-0072 ◽

2013 ◽

Vol 16 (3) ◽

pp. 517-525 ◽

Cited By ~ 9

Author(s):

A. Dziekońska ◽

L. Fraser ◽

A. Majewska ◽

M. Lecewicz ◽

Ł. Zasiadczyk ◽

...

Keyword(s):

Metabolic Activity ◽

Storage Time ◽

Membrane Integrity ◽

Prolonged Storage ◽

Atp Content ◽

Liquid Storage ◽

Semen Storage ◽

Boar Semen ◽

Boar Spermatozoa

Abstract This study was aimed to analyze the metabolic activity and membrane integrity of boar spermatozoa following storage in long-term semen extenders. Boar semen was diluted with AndrohepR EnduraGuardTM (AeG), DILU-Cell (DC), SafeCell PlusTM (SCP) and Vitasem LD (VLD) extenders and stored for 10 days at 17oC. Parameters of the analyzed sperm metabolic activity included total motility (TMOT), progressive motility (PMOT), high mitochondrial membrane potential (MMP) and ATP content, whereas those of the membrane integrity included plasma membrane integrity (PMI) and normal apical ridge (NAR) acrosome. Extender type was a significant (P < 0.05) source of variation in all the analyzed sperm parameters, except for ATP content. Furthermore, the storage time had a significant effect (P < 0.05) on the sperm metabolic activity and membrane integrity during semen storage. In all extenders the metabolic activity and membrane integrity of the stored spermatozoa decreased continuously over time. Among the four analyzed extenders, AeG and SCP showed the best performance in terms of TMOT and PMI on Days 5, 7 and 10 of storage. Marked differences in the proportions of spermatozoa with high MMP were observed between the extenders, particularly on Day 10 of storage. There were not any marked differences in sperm ATP content between the extenders, regardless of the storage time. Furthermore, the percentage of spermatozoa with NAR acrosomes decreased during prolonged storage, being markedly lower in DC-diluted semen compared with semen diluted with either AeG or SCP extender. The results of this study indicated that components of the long-term extenders have different effects on the sperm functionality and prolonged semen longevity by delaying the processes associated with sperm ageing during liquid storage.

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SENSORY QUALITY OF ROASTED COFFEE BEANS UNDER DIFFERENT STORAGE CONDITIONS

Coffee Science ◽

10.25186/cs.v14i4.1627 ◽

2019 ◽

Vol 14 (4) ◽

pp. 509

Author(s):

Denis Henrique Silva Nadaleti ◽

Hully Alves Rocha ◽

Luciana Maria Vieira Lopes Mendonça ◽

José Marcos Angélico de Mendonça ◽

Iêda Bruna dos Reis ◽

...

Keyword(s):

Storage Time ◽

Sensory Quality ◽

Storage Conditions ◽

Roasted Coffee ◽

Coffee Beans ◽

And Storage ◽

Zero Time ◽

Roasted Coffee Beans ◽

Storage Temperatures

Roasted coffee is subject to loss of quality due to aging, and the intensity of these losses is influenced by packaging. The objective in this study was to evaluate the possible losses in the sensory quality of a specialty roasted coffee, stored in beans for 150 days in different packages and storage temperatures. The experiment was carried out in the Coffee Classification and Industrialization Laboratories of IFSULDEMINAS Campus Muzambinho. The coffee was roasted and after 48 hours it was packed in three different packages and kept stored at room temperature and refrigerated at 18ºC ± 1ºC for 150 days, with evaluations every 50 days, starting from zero time. The experimental design used was entirely randomized with 3 repetitions. Sensory evaluation was performed by three Q-Grader judges, according to the SCAA protocol. The data were evaluated using the SISVAR software, and when significance between treatments was detected, the regression and Scott-Knott tests were applied at the 5% probability level. The packaging used for storage did not interfere in the quality of the coffee. There was an interaction between temperature and storage time for the sensory attribute “body”. The quality decreased linearly with the storage time, from 86 to 80 points, to 84 days of storage.

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THE EFFECT OF SODIUM PENTOBARBITAL AND STORAGE TIME ON PRESERVATION OF RAM SPERMATOZOA MOTILITY AT 5 °C

Canadian Journal of Animal Science ◽

10.4141/cjas81-104 ◽

1981 ◽

Vol 61 (4) ◽

pp. 847-851 ◽

Cited By ~ 1

Author(s):

P. S. FISER ◽

G. A. LANGFORD

Keyword(s):

Storage Time ◽

Storage Period ◽

Sodium Pentobarbital ◽

Spermatozoa Motility ◽

The Difference ◽

And Storage

The effect of sodium pentobarbital (0, 50, 100, 150, 200 and 300 mg/L of diluent) on the survival of ram spermatozoa stored at 5 °C for up to 7 days was studied. Although spermatozoa motility in diluent containing 150 mg of sodium pentobarbital was higher [Formula: see text] than in the remaining diluents, the magnitude of the difference was small. The average motility percent and rating of 73% and 4.0 for all sodium pentobarbital concentrations observed at the beginning of the storage period declined gradually to 22% and 2.1, respectively, after 7 days of storage. The pregnancy and lambing rates of 70% and 63.3% in ewes inseminated at synchronized estrus with semen extended and stored for 24 h with sodium pentobarbital (150 mg/L of diluent) did not differ significantly (P > 0.05) from the respective rates of 73% and 66.7% in the control ewes.

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Effects of long-term liquid commercial semen extender and storage time on the membrane quality of boar semen

Czech Journal of Animal Science ◽

10.17221/62/2009-cjas ◽

2010 ◽

Vol 55 (No. 4) ◽

pp. 160-166 ◽

Cited By ~ 2

Author(s):

S. Frydrychová ◽

J. Čeřovský ◽

A. Lustyková ◽

M. Rozkot

Keyword(s):

Storage Time ◽

Membrane Integrity ◽

Simple Method ◽

Laboratory Methods ◽

Significant Difference ◽

Sperm Membrane ◽

Boar Semen ◽

And Storage

The objective of this study was to assess the sperm membrane integrity in extended boar semen during storage time using specific spectrum laboratory methods. Boar semen was diluted with the long-term liquid commercial extenders Androhep (A), Androstar (AS), Androstar plus (AS+), LD and M III and was stored up to 96 h. The sperm membrane integrity was evaluated by motility, viable spermatozoa, short hypoosmotic swelling test (sHOST) and by the activity of the enzyme aspartate aminotransferase (AST). Negative changes in the quality of sperm membrane in relation to storage time were observed after 48 h for sHOST, after 72 h for viable spermatozoa and after 72 h for motility. The percentage of viable spermatozoa was decreased by 0.27% each hour. A statistically significant difference between extenders A and LD was observed in sHOST after 72 h and 96 h storage (P < 0.05). The AST activity did not show any statistically significant differences in extenders and in storage time. In overall assessment Androhep was the best of the tested extenders, followed by AS, AS+, M III and LD in terms of motility, viable spermatozoa and sHOST. The correlations among laboratory methods were highly significant (P < 0.001). In conclusion, the results documented that the sperm membrane integrity was statistically significantly affected by extenders and storage time (P < 0.001). Boar semen quality was the best in extender A. sHOST is a very sensitive and relatively simple method for the assessment of sperm membrane integrity in diluted semen.

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Artificial insemination in sows using Guelph and two local semen extenders

Nigerian Journal of Animal Production ◽

10.51791/njap.v29i1.1541 ◽

2021 ◽

Vol 29 (1) ◽

pp. 121-126

Author(s):

D. O. Umesiobi ◽

M. U. lloeje ◽

N. A. Berepubo

Keyword(s):

Litter Size ◽

Sperm Motility ◽

Artificial Insemination ◽

Coconut Water ◽

Large White ◽

Semen Characteristics ◽

Boar Semen ◽

And Storage ◽

Farrowing Rate ◽

Fresh Semen

Sixty two and half-year-old Large White sows and 12 boars of the same breed and age were used to study the comparative effects of conventional (Guelph) and local (coconut water and fresh raphia palm wine) extenders and storage (3 days) on the fertility of artificially inseminated sows. Semen was collected twice a week by gloved-hand method. Semen was either extended or unextended and evaluated on the day of collection (day 0) and during day 3 of storage for percentage sperm motility, acrosome morphology and fertility (Non-return rate, farrowing rate and litter size). Both Guelph and local extenders produced significant effects (P<0.01) on boar semen characteristics and fertility. Fresh semen extended in Guelph entender had the highest percentage sperm motility (86.2%) whereas fresh semen extended with coconut water gave the highest normal apical ridge (NAR) (91%); farrowing rate (88.3%). Litter size (7.1 piglets/litter alive (5.8), with the highest number of non return to service sows. Guelph extended semen ranked second in influencing fertility in sows.

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Efficiency of Different Diluents and Dilution Rates on the Fertilization Potential of Chicken Spermatozoa

Indian Journal of Animal Research ◽

10.18805/ijar.b-3940 ◽

2020 ◽

Author(s):

V. Beulah Pearlin ◽

J. Mohan ◽

J.S. Tyagi ◽

M. Gopi ◽

G. Kolluri ◽

...

Keyword(s):

Sperm Motility ◽

Fertile Period ◽

Semen Collection ◽

Wide Range ◽

Significant Difference ◽

Semen Storage ◽

Subsequent Storage ◽

And Storage ◽

Fertility Pattern ◽

Fresh Semen

Background: Artificial Insemination (AI) has been well established to obtain better fertility than natural mating. Semen extension and preservation play a major role in AI technique. The concentrated nature of chicken semen entails dilution of semen for easy handling and storage. With the availability of a wide range of extenders, exploring various extenders and dilution rates supplement the reproductive efficiency achieved through modern AI techniques.Methods: Pooled semen samples obtained from twenty White Leghorn breeder males were split equally into three portions, each for CARI poultry semen diluent, EK extender and Tselutin extender. At 1:2 dilution rate, the samples were subjected to sperm motility analysis from 0 to 96 h and AI was targeted at 0 and 24 h of semen collection. Fertility was assessed by candling of eggs collected from 2 to 8 days post insemination at 9th day of incubation. Further investigation of different dilution rates (1:2, 1:4, 1:6, 1:8, 1:10, 1:12, 1:14, 1:16, 1:18, 1:20) on fertility of fresh semen was done using the superior diluent.Result: Upon analysis, sperm motility showed no significant difference among the diluents at 0 h storage while CARI poultry semen diluent showed superior motility (%) at the subsequent storage periods, followed by EK and Tselutin extender. Higher fertility (p less than 0.05) was expressed in the diluents of CARI and EK at 0 h storage, whereas CARI poultry semen diluent showed superior fertility pattern (p less than 0.05) followed by EK and least by Tselutin extender at 24 h semen storage. Further investigation of different dilution rates using CARI diluent, exhibited fertility (p less than 0.05) of 90% and higher at 1:2 and 1:4 dilutions, followed by 1:6 and 1:8 at 2 to 6 days of fertile period.

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