The influence of short-term feeding of amino acid-deficient diets and high dietary leucine levels on the intramuscular fat content of pig muscle

1996 ◽  
Vol 63 (3) ◽  
pp. 517-522 ◽  
Author(s):  
F. Cisneros ◽  
M. Ellis ◽  
D. H. Baker ◽  
R. A. Easter ◽  
F. K. McKeith
Keyword(s):  
Amino Acid ◽  
Amino Acid Level ◽  
Lumbar Vertebra ◽  
Live Weight ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Longissimus Dorsi ◽  
Short Term ◽  
Pig Muscle ◽  
Intramuscular Fat Content

AbstractThe objective of the study was to investigate the influence of amino acid-deficient diets and high dietary leucine levels offered for intervals of either 21 or 35 days pre-slaughter on the intramuscular fat content of pig muscle. Twenty-four hybrid gilts were offered individually ad libitum one of four diets that comprised combinations of amino acid levels (supplemented or deficient: 5·6 v. 4·0 g lysine per kg) and low or high leucine levels (10·3 v. 30·3 g leucine per kg). Live weights at the start of the study were 74·5 and 87·0 kg for pigs on the 35- and 21-day feeding regimens, respectively. There were no significant effects of dietary amino acid level, leucine level, or feeding interval on food intake, daily live-weight gain or food efficiency. Pigs given the amino acid-deficient diets had lower killing-out proportions (736 v. 747 (s.e. 3·4) g/kg; P < 0·05) and longissimus dorsi muscle areas (32·6 v. 35·7 (s.e. 0·71) cm2, P < 0·01) than those given amino acid-supplemented diets. Subjective score for longissimus dorsi colour indicated that pigs on the high leucine diet had darker muscle than those on the low leucine diet. Intramuscular fat contents of the longissimus dors i muscle at the Wth/llth rib and the 3rd/4th lumbar vertebra and of the semimembranosu s muscle were increased by 19, 18 and 18 g/kg, respectively (P < 0·05), for pigs given amino acid-deficient compared with those given amino acid-supplemented diets. Dietary leucine level had no significant effect on intramuscular fat level. Pigs given the diets for 35, compared with 21, days had higher fat levels in the semimembranosu s muscle (52 v. 30 (s.e. 5·2) g/kg; P < 0·01) but not in the other two muscle locations studied. This study suggests that relatively short-term feeding of amino acid-deficient diets can produce substantial increases in intramuscular fat levels.

scholarly journals Data from proteomic analysis of bovine Longissimus dorsi muscle associated with intramuscular fat content

Data in Brief ◽  
2018 ◽  
Vol 19 ◽  
pp. 1314-1317 ◽  
Author(s):  
Mirele D. Poleti ◽  
Luciana C.A. Regitano ◽  
Gustavo H.M.F. Souza ◽  
Aline S.M. Cesar ◽  
Rosineide C. Simas ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Longissimus Dorsi ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Intramuscular Fat Content

scholarly journals Dietary calcium supplementation promotes the accumulation of intramuscular fat

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhiwang Zhang ◽  
Tingli Pan ◽  
Yu Sun ◽  
Siqi Liu ◽  
Ziyi Song ◽  
...  
Keyword(s):  
Meat Quality ◽  
Calcium Supplementation ◽  
Intramuscular Fat ◽  
Dietary Calcium ◽  
Fat Content ◽  
C2c12 Cells ◽  
Longissimus Dorsi ◽  
Fat Accumulation ◽  
Calcium Addition ◽  
Intramuscular Fat Content

Abstract Background In the livestock industry, intramuscular fat content is a key factor affecting meat quality. Many studies have shown that dietary calcium supplementation is closely related to lipid metabolism. However, few studies have examined the relationship between dietary calcium supplementation and intramuscular fat accumulation. Methods Here, we used C2C12 cells, C57BL/6 mice (n = 8) and three-way cross-breeding pigs (Duroc×Landrace×Large white) (n = 10) to study the effect of calcium addition on intramuscular fat accumulation. In vitro, we used calcium chloride to adjust the calcium levels in the medium (2 mmol/L or 3 mmol/L). Then we measured various indicators. In vivo, calcium carbonate was used to regulate calcium levels in feeds (Mice: 0.5% calcium or 1.2% calcium) (Pigs: 0.9% calcium or 1.5% calcium). Then we tested the mice gastrocnemius muscle triglyceride content, pig longissimus dorsi muscle meat quality and lipidomics. Results In vitro, calcium addition (3 mmol/L) had no significant effect on cell proliferation, but promoted the differentiation of C2C12 cells into slow-twitch fibers. Calcium supplementation increased triglyceride accumulation in C2C12 cells. Calcium addition increased the number of mitochondria and also increased the calcium level in the mitochondria and reduced the of key enzymes activity involved in β-oxidation such as acyl-coenzyme A dehydrogenase. Decreasing mitochondrial calcium level can alleviate lipid accumulation induced by calcium addition. In addition, calcium addition also reduced the glycolytic capacity and glycolytic conversion rate of C2C12 cells. In vivo, dietary calcium supplementation (1.2%) promoted the accumulation of triglycerides in the gastrocnemius muscle of mice. Dietary calcium supplementation (1.5%) had no effect on pig weight, but significantly improved the flesh color of the longissimus dorsi muscle, reduced the backfat thickness and increased intramuscular fat content in pigs. Besides, calcium addition had no effect on longissimus dorsi pH, electrical conductivity and shear force. Conclusions These results suggest that calcium addition promotes intramuscular fat accumulation by inhibiting the oxidation of fatty acids. These findings provide a new tool for increasing intramuscular fat content and an economical strategy for improving meat quality.

scholarly journals Efect of intramuscular fat content on physical-chemical parameters of pork from Mangalitsa and their crossbreed

10.5219/1095 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 422-428
Author(s):  
Petra Lípová ◽  
Ondrej Debrecéni ◽  
Ondřej Bučko ◽  
Klára Vavrišínová
Keyword(s):  
Moisture Content ◽  
Protein Content ◽  
Live Weight ◽  
Cholesterol Content ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Post Mortem ◽  
Chemical Parameters ◽  
Large White ◽  
Intramuscular Fat Content

The aim of study was to evaluate the effect of intramuscular fat content on physical parameters and proximate composition in musculus longissimus dorsi (MLD) from Mangalitsa breed and Slovak Large White x Mangalitsa crossbreed. In the study, sixteen pigs of Mangalitsa and twenty-two pigs of Slovak Large White x Mangalitsa crossbreed were used. The pigs were reared under intensive condition and all animals were fed ad libitum with complete fattening feed mixture. The fattening period started from 30 kg of live weight. Then the pigs were slaughtered at 100 kg of live weight. Chemical analysis showed that MLD from Mangalitsa had lower protein content, higher moisture content (p <0.05) and higher content of intramuscular fat compared to Slovak Large White x Mangalitsa crossbreed. As regarding the cholesterol content in MLD, no significant differences were found between genotypes, but the cholesterol content was higher in MLD from Mangalitsa than in MLD from crossbreeds. The MLD from Mangalitsa exhibited lower CIE L* (p <0.01) and CIE b* (p <0.01) values 45 min post mortem compared to crossbreeds. Colour parameters increased after 7 days post mortem, which is normal due to the maturing process of the meat. Then the CIE L* value was lower in MLD from Mangalitsa (p <0.01), but CIE a* value was higher in relation to crossbreeds (p <0.05). As regards the Warner-Bratzler shear force, the meat from Mangalitsa was tenderer than in crossbreeds (p <0.05). Intramuscular fat in the meat positively correlated with colour parameter CIE a* (r = 0.324; p <0.05) as well as cholesterol content (r = 0,656; p <0.001). In contrast in the study was found negative correlations between intramuscular fat in meat with moisture content (r = -0.399; p <0.05) and protein content (r = -0.812; p <0.001). It can be concluded that the percentage of intramuscular fat significantly influenced the physical and the chemical parameters of pork. The meat from Mangalitsa is more suitable for production of special meat products (fermented and smoked).

The influence of breed and type of feed on the cholesterol content of the M. longissimus dorsi of steers

1990 ◽  
Vol 30 (6) ◽  
pp. 797 ◽  
Author(s):  
DG Taylor ◽  
LW Smith
Keyword(s):  
Correlation Coefficients ◽  
Cholesterol Content ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Longissimus Dorsi ◽  
Fat Thickness ◽  
Intramuscular Fat Content

The cholesterol content of muscle from pasture-fed Hereford (n = 21) and Brahman (n = 12) steers, and from grain-fed Hereford (n = 20) steers was determined. No significant differences (P>0.05) were detected in the cholesterol content of muscle between breeds or between types of feed. Small, non-significant correlation coefficients were detected between cholesterol content of muscle and carcass fat thickness, while the association between cholesterol content of muscle and intramuscular fat content was stronger but only significant (P<0.05) for the Brahman group. Thus, breed, feed type and possibly carcass fatness were not variables that could be used to reduce the cholesterol content of muscle.

COMPARATIVE ASSESSMENT OF PRODUCTIVE CHARACTERISTICS PURE-BREED AND MIXED BRED STORE PIGS OF CANADIAN SELECTION

1970 ◽  
pp. 78-80
Author(s):  
L. А. Morozova ◽  
I. N. Mikolaychik ◽  
V. G. Chumakov ◽  
А. S. Neupokoeva
Keyword(s):  
Dry Matter ◽  
Live Weight ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Comparative Assessment ◽  
Cross Breeding ◽  
Pure Breed ◽  
Young Pigs ◽  
Intramuscular Fat Content

The aim of the research was to compare the productive qualities of the purebred and cross-breeding young pigs on the fattening of Canadian breeding in two- and three-breed crossings. Object of the study: youngsters of the Landras pigs, two-breed hybrids (Landras x Yorkshire), three-breeders (Landras x Yorkshire x Duroc), imported from Canada to the KFKH "Il'tyakov VN", Oftenozero district of the Kurgan region. The results of the experiment showed that under the same feeding conditions and the content of purebred, two- and three-breeded young pigs of Canadian breeding, differences in the character of the change in the dynamics of the live mass are observed. The gross increase in live weight was the highest in three-breed hybrids and amounted to 128.32 kg, which is 4.27% (P <0.05) higher than for pure-landrace. The age of reaching a live weight of 100 kg was the smallest in the three-breed hybrids in comparison with the two-breed hybrids and purebred landrace at 2.69 and 5.83 days. The slaughter yield in tri-tribe hybrids exceeded the purebred landrace by 1.25% (P <0.01). The weight of the paired carcass was also higher in tri-hybrids compared to the bipedal hybrids at 2.49 and purebred landrasas - by 7.36% (P <0.05). The highest content of dry matter was characterized by meat obtained from pure-earthland, while the protein and intramuscular fat content did not differ significantly and was within the range of 22.35-22.45% and 2.52-2.55%, respectively.

scholarly journals Gene expression profile of Musculus longissimus dorsi in bulls of a Charolais × Holstein F 2 -cross with divergent intramuscular fat content

Genomics Data ◽  
2016 ◽  
Vol 7 ◽  
pp. 131-133 ◽  
Author(s):  
Katrin Komolka ◽  
Siriluck Ponsuksili ◽  
Elke Albrecht ◽  
Christa Kühn ◽  
Klaus Wimmers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Longissimus Dorsi ◽  
Intramuscular Fat Content
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