Effects of age and maternal reactivity on the stress response of the pituitary-adrenocortical axis and the sympathetic nervous system in neonatal pigs

1999 ◽  
Vol 68 (3) ◽  
pp. 519-526 ◽  
Author(s):  
E. Kanitz ◽  
W. Otten ◽  
G. Nürnberg ◽  
K. P. Brüssow
Keyword(s):  
Stress Reactivity ◽  
Acth Stimulation Test ◽  
Acth Stimulation ◽  
Acth Challenge ◽  
Neonatal Pigs ◽  
Age Related ◽  
Highly Sensitive ◽  
Function Test ◽  
Adrenal Response ◽  
Different Levels

AbstractThe study was conducted to investigate the adreno cortical capacity after injection of ACTH and the sensitivity of the pituitary and the adrenal to immobilization in neonatal pigs at different ages. Furthermore, the endocrine reactivity of the offspring was compared with the stress reactivity of their mothers. Four piglets were selected from each of six different litters and subjected to an immobilization test and an adrenal function test using synthetic ACTH1-24 at the ages of 7, 21 and 35 days; the six sows were also subjected to restraint and an ACTH stimulation test. Plasma β-endorphin, norepinephrine and epinephrine concentrations were measured in blood samples taken 2 min after restraint and cortisol concentrations were measured 60 min after ACTH administration. A highly sensitive adrenal response was demonstrated in both sows and piglets and adrenal reactivity showed also a considerable consistency over time within sows. In neonatal pigs, the cortisol response to ACTH was greatest on day 7 and decreased up to day 35. Plasma epinephrine and norepinephrine levels after the 2-min immobilization were also higher at day 7 compared with the other ages (P < 0·01). Piglets from sows, classified as high reacting according to their cortisol or epinephrine response, also showed significantly higher cortisol levels after ACTH challenge at all ages and significantly higher epinephrine levels after restraint at day 7 than piglets from low reacting sows. The results show an age-related change of pituitary-adrenocortical and sympatho-adrenomedullary responses in neonatal pigs and an absence of a stress hyporesponsive period at all ages studied. The results also indicate different levels of excitability in the offspring depending on the maternal stress reactivity.

scholarly journals Effects of breed, management and personality on cortisol reactivity in sport horses

2019 ◽  
Author(s):  
Fay J. Sauer ◽  
Marco Hermann ◽  
Alessandra Ramseyer ◽  
Dominik Burger ◽  
Stefanie Riemer ◽  
...  
Keyword(s):  
Personality Traits ◽  
Adrenocorticotropic Hormone ◽  
Linear Models ◽  
Stress Reactivity ◽  
Acth Stimulation Test ◽  
Acth Stimulation ◽  
Cortisol Reactivity ◽  
Amateur Sport ◽  
Adrenal Response ◽  
Management Factors

AbstractSport horses need to fulfill high physical and psychological requirements during training and competition. These as well as certain conditions of modern husbandry may affect their wellbeing. Here we aimed to (1) evaluate effects of demographic and management factors as well as personality traits on stress reactivity of sport horses, (2) investigate if elite sport horses have elevated stress levels compared to amateur sport horses, and (3) assess whether different equestrian disciplines differentially influence horses’ adrenal cortex responsiveness. For this purpose, we visited 149 healthy elite (n=94) and amateur (n=54) sport horses in Switzerland and performed an adrenocorticotropic hormone (ACTH) stimulation test. Additionally, a person who was familiar with the horse completed a questionnaire about demographic and management factors and horses’ personality traits. Linear models were calculated to assess associations between the questionnaire data and salivary cortisol 60 and 90 minutes after ACTH stimulation. While the model at T90 was not significant, post-stimulatory cortisol after 60 min appears most informative in line with a previous study and was significantly affected by the horses’ breed and by three management factors: “number of riders”, “hours spent outside” and “group housing” (adjusted r2 =15%, p<0.001). Thoroughbred and Warmblood horses displayed an increased adrenal response compared to Franches-Montagnes horses. Horses with several riders had a less pronounced reaction than horses with one rider, and horses that spent more time outside had a decreased response compared to horses that were stabled most of the time. Horses living in groups showed higher post-stimulatory cortisol values than horses that were housed singly. However, no significant associations of cortisol responsiveness with personality traits were found, and neither the horses’ use as elite or as amateur sport horses nor the discipline had an effect on the cortisol response. This suggests that optimizing husbandry conditions may be more important for improving horses’ welfare than changing their use.

Reduced responsiveness of the hypothalamic-pituitary-adrenal axis in Boran (Bos indicus) cattle infected with Trypanosoma congolense

1993 ◽  
Vol 129 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Getachew Abebe ◽  
Robert M Eley ◽  
Onesmo K ole-MoiYoi
Keyword(s):  
Bos Indicus ◽  
Trypanosoma Congolense ◽  
Cortisol Concentration ◽  
Post Treatment ◽  
Acth Stimulation ◽  
Acth Challenge ◽  
Adrenal Axis ◽  
Pituitary Dysfunction ◽  
Adrenal Response ◽  
Pituitary Adrenal Axis

The response of the pituitary-adrenal axis to corticotrophin-releasing hormone (CRH) and the adrenal response to adrenocorticotrophin hormone (ACTH) stimulation were studied during infection of Boran (Bos indicus) cattle with Trypanosoma congolense. For CRH, 15 animals were challenged during pre-infection and infection phases, while for ACTH 10 animals were challenged during pre-infection, infection and post-treatment phases of the experiments. The axis showed a reduced responsiveness after CRH challenge during patent parasitaemia, manifested by low (p<0.05) plasma ACTH and cortisol concentration as compared to the pre-infection CRH challenge concentrations. Cortisol concentration after ACTH challenge during pre-infection, infection and post-treatment phases did not differ. The reduced pituitary-adrenal response to CRH challenge and the normal adrenal response to ACTH challenge found during trypanosomiasis indicates pituitary dysfunction due to infection.

DETERMINATION OF BLOOD CORTICOIDS USING THE IN VITRO RESIN UPTAKE OF 3H-PREDNISOLONE

1968 ◽  
Vol 57 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Hironori Nakajima ◽  
Mitsunori Murala ◽  
Masumitsu Nakata ◽  
Takeshi Naruse ◽  
Seiji Kubo
Keyword(s):  
Thyroid Function Test ◽  
Normal Subjects ◽  
Adrenal Function ◽  
Function Test ◽  
Before And After ◽  
Adrenal Response ◽  
Suppression Test

ABSTRACT The in vitro resin uptake of 3H-prednisolone was used for the determination of blood cortisol after addition of radioactive prednisolone followed by Amberlite CG 400 Type 1 to the test serum, and incubation of the mixture. The radioactivity of the supernatant was compared before and after the addition of the resin. The principle of this method is similar to that of the 131I-triiodothyronine resin uptake for the thyroid function test. The tests for the specificity, reproducibility and sensitivity gave satisfactory results. The mean basal value ± SD of the 3H-prednisolone resin uptake was 35.3 ± 9.2% in normal subjects, and 27.1 ± 4.8% in pregnant women. This method was valid in various adrenal function tests, i. e. the adrenal circadian rhythm, corticotrophin (ACTH) test, dexamethasone suppression test and the adrenal response to lysine-8-vasopressin. It proved to be a sensitive indicator of the adrenal function. These results suggest that this method should be useful for a routine adrenal function test.

scholarly journals Mingjing granule, a traditional Chinese medicine in the treatment of neovascular age-related macular degeneration: study protocol for a randomized controlled trial

Trials ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yamin Li ◽  
Lina Liang ◽  
Torkel Snellingen ◽  
Kai Xu ◽  
Yun Gao ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Macular Degeneration ◽  
Vision Loss ◽  
Age Related Macular Degeneration ◽  
Case Report Form ◽  
Link Type ◽  
Anti Vegf ◽  
Age Related ◽  
Function Test

Abstract Background Neovascular age-related macular degeneration (nAMD) is the most common cause of irreversible vision loss and blindness among the older people aged 50 and over. Although anti-vascular endothelial growth factor (anti-VEGF) therapies have resulted in improving patient outcomes, there are limitations associated with these treatments. In China, traditional Chinese medicine (TCM) has been used to treat eye diseases for more than 2000 years. Previous studies have shown that TCM may be beneficial for nAMD patients. However, explicit evidence has not been obtained. The purpose of the present trial is to examine the efficacy and safety of the Mingjing granule, a compound Chinese herbal medicine, for nAMD patients. Methods/design This is a double-blind, placebo-controlled, randomized trial of Mingjing granule as an add-on to intravitreous ranibizumab for nAMD. One hundred eighty nAMD patients from six hospitals in China will be enrolled according to the inclusion and exclusion criteria and randomly allocated into two groups, 90 in each. All participants will receive a 24-week treatment and then be followed up for another 24 weeks. The primary outcome is the mean change of best-corrected visual acuity at week 24 and 48 as compared to the baseline. The secondary outcomes include mean change in central retinal thickness, area of retinal hemorrhage and exudation, and TCM syndrome score, mean number of intravitreal ranibizumab injection, and total cost of the treatment. Indexes of safety include blood regular test, urine regular test, liver function test, renal function test, and electrocardiogram from baseline to weeks 24 and 48. Qualitative control and some standard operating processes will be formed throughout the trial. Any ocular or systemic adverse events will be treated suitably, and related data will be recorded accurately and completely in the case report form. Discussion Based on previous empirical and animal laboratory studies, this study will address the question of whether Mingjing granule could contribute to improving efficacy, safety, and efficiency with need for fewer intravitreal injections of anti-VEGF, improving compliance and visual outcomes in the management of persons with nAMD. Trial registration Chinese Clinical Trial Registry (http://www.chictr.org.cn), ChiCTR2000035990. Registered on 21 August 2020.

scholarly journals Diagnostic Value of ACTH Stimulation Test in Determining the Subtypes of Primary Aldosteronism

2015 ◽  
Vol 100 (5) ◽  
pp. 1837-1844 ◽  
Author(s):  
Yiran Jiang ◽  
Cui Zhang ◽  
Weiqing Wang ◽  
Tingwei Su ◽  
Weiwei Zhou ◽  
...  
Keyword(s):  
Primary Aldosteronism ◽  
Diagnostic Value ◽  
Stimulation Test ◽  
Acth Stimulation Test ◽  
Acth Stimulation

Adrenocortical responsiveness to the ACTH stimulation test in depressed patients and healthy volunteers

1986 ◽  
Vol 11 (3) ◽  
pp. 265-274 ◽  
Author(s):  
Jay D. Amsterdam ◽  
Greg Maislin ◽  
Ellen Abelman ◽  
Neil Berwish ◽  
Andrew Winokur
Keyword(s):  
Healthy Volunteers ◽  
Stimulation Test ◽  
Acth Stimulation Test ◽  
Acth Stimulation ◽  
Depressed Patients

scholarly journals Cytochemical Demonstration of Oxidative Damage in Alzheimer Disease by Immunochemical Enhancement of the Carbonyl Reaction with 2,4-Dinitrophenylhydrazine

1998 ◽  
Vol 46 (6) ◽  
pp. 731-735 ◽  
Author(s):  
Mark A. Smith ◽  
Lawrence M. Sayre ◽  
Vernon E. Anderson ◽  
Peggy L.R. Harris ◽  
M. Flint Beal ◽  
...  
Keyword(s):  
Alzheimer Disease ◽  
Oxidative Damage ◽  
Peroxidation Of Lipids ◽  
Bulk Analysis ◽  
Age Related ◽  
Cytochemical Demonstration ◽  
Highly Sensitive ◽  
Metal Catalyzed ◽  
Specific Oxidation ◽  
Cytochemical Technique

Formation of carbonyls derived from lipids, proteins, carbohydrates, and nucleic acids is common during oxidative stress. For example, metal-catalyzed, “site-specific” oxidation of several amino acid side-chains produces aldehydes or ketones, and peroxidation of lipids generates reactive aldehydes such as malondialdehyde and hydroxynonenal. Here, using in situ 2,4-dinitrophenylhydrazine labeling linked to an antibody system, we describe a highly sensitive and specific cytochemical technique to specifically localize biomacromolecule-bound carbonyl reactivity. When this technique was applied to tissues from cases of Alzheimer disease, in which oxidative events including lipoperoxidative, glycoxidative, and other oxidative protein modifications have been reported, we detected free carbonyls not only in the disease-related intraneuronal lesions but also in other neurons. In marked contrast, free carbonyls were not found in neurons or glia in age-matched control cases. Importantly, this assay was highly specific for detecting disease-related oxidative damage because the site of oxidative damage can be assessed in the midst of concurrent age-related increases in free carbonyls in vascular basement membrane that would contaminate biochemical samples subjected to bulk analysis. These findings demonstrate that oxidative imbalance and stress are key elements in the pathogenesis of Alzheimer disease.

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