scholarly journals Microvascular Architecture of the Mouse Urinary Bladder Described with Vascular Corrosion Casting, Light Microscopy, SEM, and TEM

2009 ◽  
Vol 15 (S2) ◽  
pp. 984-985 ◽  
Author(s):  
F Hossler ◽  
A Lametschwandtner ◽  
R Kao ◽  
C Bills ◽  
F Finsterbusch
Keyword(s):  
Urinary Bladder ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Sem And Tem ◽  
Microvascular Architecture

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

Microvascular Architecture of Mouse Urinary Bladder Described with Vascular Corrosion Casting, Light Microscopy, SEM, and TEM

2013 ◽  
Vol 19 (6) ◽  
pp. 1428-1435 ◽  
Author(s):  
Fred E. Hossler ◽  
Alois Lametschwandtner ◽  
Race Kao ◽  
Friederike Finsterbusch
Keyword(s):  
Urinary Bladder ◽  
Light Microscopy ◽  
Collateral Circulation ◽  
Normal Function ◽  
Genetic Models ◽  
Corrosion Casting ◽  
Sem And Tem ◽  
Capillary Plexus ◽  
Human Disorders ◽  
Microvascular Architecture

AbstractThe urinary bladder is a unique organ in that its normal function is storage and release of urine, and vasculature in its wall exhibits specialized features designed to accommodate changes in pressure with emptying and filling. Although we have previously described the fine details of the microvasculature of the urinary bladder of the rabbit and dog, information on the fine details of the microvasculature of the mouse bladder were deemed to be of value because of the increasing use of this species in developing genetic models for studying human disorders. The present study shows that many of the special features of the microvasculature of the mouse urinary bladder are similar to those described in the rabbit and dog, including vessel coiling, abundant collateral circulation, arterial sphincters, and a dense mucosal capillary plexus.

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

1995 ◽  
Vol 53 ◽  
pp. 1074-1075
Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson
Keyword(s):  
Lymph Nodes ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Fixed Tissue ◽  
Infusion Pressure ◽  
India Ink ◽  
Cacodylate Buffer ◽  
Rabbit Bladder ◽  
Lateral Walls ◽  
Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

3D-Morphometry of the Microvascular Architecture in Normal Human Tissues, Precanceroses, and Primary Tumors: Quantitative Corrosion Casting Studies

2000 ◽  
Vol 6 (S2) ◽  
pp. 566-567
Author(s):  
M. A. Konerding ◽  
W. Malkusch ◽  
E. Fait ◽  
A. Gaumann
Keyword(s):  
Corrosion Casting ◽  
Tumor Type ◽  
Colorectal Carcinomas ◽  
Experimental Tumors ◽  
Primary Tumors ◽  
Vascular Architecture ◽  
Microcorrosion Casts ◽  
Microvascular Architecture ◽  
Colon And Rectum ◽  
Normal Human

The important question as to whether the vascular architecture of an individual tumor is tumor type specific has been controversial. We have recently shown using a simple and accurate technique for 3D-measurements in microcorrosion casts that at least experimental tumors develop a characteristic vascular network. There is strong evidence that the basic vascular architecture is determined by the tumor cells themselves. However, for human primary tumors and their metastases as well as their possible precanceroses no data are available until today.Therefore we studied the microvascular architecture of different colorectal carcinomas and adenomas as well as the vascularity of different segments of the colon and rectum. For this, parameters defining the microvascular unit were analysed on 3D reconstructed images as recently described.Comparisons of the quantified parameters within a given segment of the colon as well as group comparisons of all segments did not reveal any significant inter-individual difference.

scholarly journals Morphomolecular motifs of pulmonary neoangiogenesis in interstitial lung diseases

2019 ◽  
Vol 55 (3) ◽  
pp. 1900933 ◽  
Author(s):  
Maximilian Ackermann ◽  
Helge Stark ◽  
Lavinia Neubert ◽  
Stephanie Schubert ◽  
Paul Borchert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Blood Vessels ◽  
Expression Analysis ◽  
Lung Diseases ◽  
Gene Expression Analysis ◽  
Interstitial Lung Diseases ◽  
Corrosion Casting ◽  
Cellular Functions ◽  
Microvascular Architecture ◽  
Nonspecific Interstitial Pneumonia

The pathogenetic role of angiogenesis in interstitial lung diseases (ILDs) is controversial. This study represents the first investigation of the spatial complexity and molecular motifs of microvascular architecture in important subsets of human ILD. The aim of our study was to identify specific variants of neoangiogenesis in three common pulmonary injury patterns in human ILD.We performed comprehensive and compartment-specific analysis of 24 human lung explants with usual intersitial pneumonia (UIP), nonspecific interstitial pneumonia (NSIP) and alveolar fibroelastosis (AFE) using histopathology, microvascular corrosion casting, micro-comupted tomography based volumetry and gene expression analysis using Nanostring as well as immunohistochemistry to assess remodelling-associated angiogenesis.Morphometrical assessment of vessel diameters and intervascular distances showed significant differences in neoangiogenesis in characteristically remodelled areas of UIP, NSIP and AFE lungs. Likewise, gene expression analysis revealed distinct and specific angiogenic profiles in UIP, NSIP and AFE lungs.Whereas UIP lungs showed a higher density of upstream vascularity and lower density in perifocal blood vessels, NSIP and AFE lungs revealed densely packed alveolar septal blood vessels. Vascular remodelling in NSIP and AFE is characterised by a prominent intussusceptive neoangiogenesis, in contrast to UIP, in which sprouting of new vessels into the fibrotic areas is characteristic. The molecular analyses of the gene expression provide a foundation for understanding these fundamental differences between AFE and UIP and give insight into the cellular functions involved.

Microvasculature of the Urinary Bladder of the Dog: A Study Using Vascular Corrosion Casting

2007 ◽  
Vol 13 (3) ◽  
pp. 220-227 ◽  
Author(s):  
Fred E. Hossler ◽  
Race L. Kao
Keyword(s):  
Blood Flow ◽  
Urinary Bladder ◽  
Bladder Wall ◽  
Three Dimensional ◽  
Capillary Density ◽  
Normal Function ◽  
Bladder Function ◽  
Corrosion Casting ◽  
Normal Bladder ◽  
And Control

The urinary bladder is an unusual organ in that its normal function includes filling and emptying with alternating changes in internal pressure. Although fluctuations in blood flow to the bladder wall are known to accompany these changes, detailed descriptions of the bladder microvasculature are sparse. The present study uses vascular corrosion casting and scanning electron microscopy to describe the three-dimensional anatomy of the microvasculature of the urinary bladder of the dog. Specialized features of that microvasculature, including collateral circulation, vessel folding, vessel orientation, the presence of valves and sphincters, and mucosal capillary density, that may enhance and control blood flow during normal bladder function, are described and discussed.

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