scholarly journals Using a Formvar-Coated Bridge to Apply Formvar Support Film to TEM Grids

1999 ◽  
Vol 7 (5) ◽  
pp. 18-19
Author(s):  
John P. Shields
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Conventional Method ◽  
Serial Sections ◽  
University Of Georgia ◽  
Alternative Method ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
The University

One conventional method for picking up ultrathin sections for transmission electron microscopy (TEM) is to prepare the mesh or slot grids with a formvar support film and then pick up your sections as they are floating in the knife boat. An alternative method is to first pick up sections on a naked grid and then lay them down on formvar film suspended over holes drilled in an aluminum bridge. I use this method when picking up serial sections on slot grids. A description of the method follows. The bridges I have were manufactured at the University of Georgia instrumentation Shop. They can also be purchased pre-made (for example, from SPI, Inc. They list them as Domino Racks), or one can make them from aluminum with a vise and drill. The sample bridge in the figure is similar to what I use. It is 5 cm long, 2.5 cm wide.

scholarly journals Method for Combined Observation of Serial Sections of Stented Arteries Embedded in Resin by Light Microscopy and Transmission Electron Microscopy

2018 ◽  
Vol 47 (3) ◽  
pp. 401-407 ◽  
Author(s):  
Atsushi Isobe ◽  
Kouichi Iwatani ◽  
Junko Souba ◽  
Hisako Terao ◽  
Hitomi Hagiwara ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Periodic Acid ◽  
Cell Junctions ◽  
Mixed Solution ◽  
Serial Sections ◽  
Thin Sections ◽  
Transmission Electron ◽  
Ultrathin Sections

We have developed a new method for obtaining information on whole tissues by light microscopy (LM) and ultrastructural features by transmission electron microscopy (TEM). This method uses serial sections of a stented artery embedded in resin. Stents were implanted in porcine coronary arteries in this study. The heart was perfusion fixed in a 2% paraformaldehyde and 1.25% glutaraldehyde mixed solution. The stented artery was then removed, fixed in 1% osmium, embedded in Quetol 651 resin, and sectioned serially. For LM, the black color of osmium was removed from the section by immersion in periodic acid and hydrogen peroxide after deplasticization. These sections were stained with hematoxylin and eosin and Elastica–Masson trichrome stain. For TEM, thin sections were re-embedded in Quetol 812 resin by the resupinate method and cut into ultrathin sections. A clear, fine structure was obtained, and organelles, microvilli, and cell junctions in the endothelium were easily observed. The combined observation of adjacent specimens by LM and TEM enabled us to relate histopathological changes in the millimeter scale to those in the nanometer scale.

The 1-MeV Electron Microscope Installation at the University of Colorado

1973 ◽  
Vol 31 ◽  
pp. 8-9 ◽  
Author(s):  
Mircea Fotino
Keyword(s):  
Electron Microscopy ◽  
Developmental Biology ◽  
Transmission Electron Microscopy ◽  
Electron Microscope ◽  
National Institutes Of Health ◽  
Experimental Program ◽  
University Of Colorado ◽  
Transmission Electron ◽  
The University ◽  
Research Resources

A new 1-MeV transmission electron microscope (Model JEM-1000) was installed at the Department of Molecular, Cellular and Developmental Biology of the University of Colorado in Boulder during the summer and fall of 1972 under the sponsorship of the Division of Research Resources of the National Institutes of Health. The installation was completed in October, 1972. It is installed primarily for the study of biological materials without many of the limitations hitherto unavoidable in standard transmission electron microscopy. Only the technical characteristics of the installation are briefly reviewed here. A more detailed discussion of the experimental program under way is being published elsewhere.

Magnesium and sulfur in mast cell and basophil granules of lower vertebrates in relation with histamine

1992 ◽  
Vol 50 (2) ◽  
pp. 1596-1597
Author(s):  
Kenichi Takaya
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Tree Frog ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Fresh Frozen ◽  
Ultrathin Sections

Mast cell and basophil granules of the vertebrate contain heparin or related sulfated proteoglycans. Histamine is also present in mammalian mast cells and basophils. However, no histamine is detected in mast cell granules of the amphibian or fish, while it is shown in those of reptiles and birds A quantitative x-ray microanalysis of mast cell granules of fresh frozen dried ultrathin sections of the tongue of Wistar rats and tree frogs disclosed high concentrations of sulfur in rat mast cell granules and those of sulfur and magnesium in the tree frog granules. Their concentrations in tree frog mast cell granules were closely correlated (r=0.94).Fresh frozen dried ultrathin sections and fresh air-dried prints of the tree frog tongue and spleen and young red-eared turtle (ca. 6 g) spleen and heart blood were examined by a quantitative energy-dispersive x-ray microanalysis (X-650, Kevex-7000) for the element constituents of the granules of mast cells and basophils. The specimens were observed by transmission electron microscopy (TEM) (80-200 kV) and followed by scanning transmission electron microscopy (STEM) under an analytical electron microscope (X-650) at an acceleration voltage of 40 kV and a specimen current of 0.2 nA. A spot analysis was performed in a STEM mode for 100 s at a specimen current of 2 nA on the mast cell and basophil granules and other areas of the cells. Histamine was examined by the o-phthalaldehyde method.

scholarly journals Recent Advances in Transmission Electron Microscopy for Materials Science at the EMAT Lab of the University of Antwerp

Materials ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 1304 ◽  
Author(s):  
Giulio Guzzinati ◽  
Thomas Altantzis ◽  
Maria Batuk ◽  
Annick De Backer ◽  
Gunnar Lumbeeck ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Materials Science ◽  
Electron Tomography ◽  
High Resolution Imaging ◽  
Rapid Progress ◽  
Transmission Electron ◽  
The World ◽  
Resolution Imaging ◽  
The University

The rapid progress in materials science that enables the design of materials down to the nanoscale also demands characterization techniques able to analyze the materials down to the same scale, such as transmission electron microscopy. As Belgium’s foremost electron microscopy group, among the largest in the world, EMAT is continuously contributing to the development of TEM techniques, such as high-resolution imaging, diffraction, electron tomography, and spectroscopies, with an emphasis on quantification and reproducibility, as well as employing TEM methodology at the highest level to solve real-world materials science problems. The lab’s recent contributions are presented here together with specific case studies in order to highlight the usefulness of TEM to the advancement of materials science.

Postero-Lateral Glands of Temnocephala Minor Haswell, 1888 (Platyhelminthes: Temnocephalida)

1996 ◽  
Vol 44 (1) ◽  
pp. 69
Author(s):  
LRG Cannon ◽  
NA Warson
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Lateral Margin ◽  
Freshwater Crayfish ◽  
Cherax Destructor ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Whole Mounts

Temnocephala minor Haswell, 1888 lives ectosymbiotically on the surface of the freshwater crayfish Cherax destructor in the Murray-Darling drainages of Australia. Some glands open on the postero-lateral margin and, being moderately refractory to many stains, can be overlooked in whole mounts and sections, and were, in fact, missed by Haswell. Observations were made on living worms with intra vitam dyes, and on whole mounts, wax sections and ultrathin sections using transmission electron microscopy (TEM) to characterise the secretion from these glands and ascertain its mode of manufacture. The function of the glands remains unknown although it appears non-adhesive.

Interface Structure and Layer Synthesis Modes in Mesotaxial Si/CoSi2/Si Structures

1990 ◽  
Vol 183 ◽  
Author(s):  
R. Hull ◽  
Y. F. Hsieh ◽  
K. T. Short ◽  
A. E. White ◽  
D. Cherns
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Resolution ◽  
Lattice Parameter ◽  
Interface Structure ◽  
Interfacial Structure ◽  
Parameter Mismatch ◽  
Alternative Method ◽  
Transmission Electron

AbstractWe report observations of interfacial structure and consequences for layer synthesis modes in mesotaxial Si/CoSi2/Si structures, as deduced from high resolution transmission electron microscopy (HRTEM). It is argued that relative crystal misalignments arising from the lattice parameter mismatch between the Si and CoSi2 may render classic rigid shift measurements of interfacial structure inaccurate. An alternative method for determining interfacial structure at threedimensional precipitates by analyzing crystal stacking sequences is demonstrated.

The Technology in Sample Preparation for Transmission Electron Microscopy and Ultrastructure Observation of Lung Tissue in Rats with Experimental Silicosis

2014 ◽  
Vol 875-877 ◽  
pp. 695-698
Author(s):  
Qian Li ◽  
Li Juan Zhang ◽  
Fang Yang ◽  
Wen Li Zhang ◽  
Hong Xu
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Sample Preparation ◽  
Success Rate ◽  
Cutting Speed ◽  
Ultrastructural Changes ◽  
Histological Changes ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Perfusion Fixation

ts hard to get ideal ultrathin sections because of the adamant SiO2 dust in silicosis, after perfusion fixation methods and strict control of the cutting speed, improving the success rate of the Silicosis tissue TEM sample preparation of ultrathin sections,so we can more clearly and accurately observed ultrastructural changes of silicosis,and it also can offer morphological basis for research the silicosis organizations function histological changes.

scholarly journals Same Serial Section Correlative Light and Energy-filtered Transmission Electron Microscopy

2003 ◽  
Vol 51 (5) ◽  
pp. 605-612 ◽  
Author(s):  
Ying Ren ◽  
Michael J. Kruhlak ◽  
David P. Bazett-Jones
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscope ◽  
High Rate ◽  
Specific Cell ◽  
3D Analysis ◽  
Serial Sections ◽  
Correlative Imaging ◽  
Transmission Electron ◽  
Rate Of Success

Correlative imaging of a specific cell with both the light microscope and the electron microscope has proved to be a difficult task, requiring enormous amounts of patience and technical skill. We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy. The technique also includes a method to obtain many uninterrupted, thin serial sections for imaging by conventional or energy-filtered transmission electron microscopy, to obtain images for 3D analysis of detail at the suborganelle level.

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