Image and Diffraction Pattern Rotations in the TEM

2012 ◽  
Vol 20 (5) ◽  
pp. 52-55
Author(s):  
Graham J.C. Carpenter
Keyword(s):  
Electron Microscope ◽  
Diffraction Pattern ◽  
Transmission Electron Microscope ◽  
Image Rotation ◽  
Spiral Path ◽  
Transmission Electron ◽  
Pass Through ◽  
Computer Processor ◽  
Focused Beam ◽  
Imaging Plane

When electrons pass through the electromagnetic lenses in a transmission electron microscope (TEM), they follow a spiral path that results in image rotation. In many TEMs, the image or diffraction pattern that appears at the final imaging plane has therefore suffered a significant rotation compared to the actual specimen. The extent of the rotation is equal to the sum of the contributions from each lens. In some recent instruments an extra lens is built into the column to compensate for these rotations. In the case of a scanning TEM (STEM), where the image is created by scanning a focused beam on the specimen, the orientation of the image to the specimen is fixed but can be controlled electronically by the computer processor.

Using the TEM Condenser Lens to Switch between Image and Diffraction Modes

2013 ◽  
Vol 21 (2) ◽  
pp. 40-40
Author(s):  
Lydia Rivaud
Keyword(s):  
Electron Microscope ◽  
Diffraction Pattern ◽  
Transmission Electron Microscope ◽  
Condenser Lens ◽  
Selected Area Diffraction Pattern ◽  
Transmission Electron ◽  
Set Up ◽  
Convergent Beam ◽  
Beam Diffraction ◽  
Convergent Beam Diffraction

Central to the operation of the transmission electron microscope (TEM) (when used with crystalline samples) is the ability to go back and forth between an image and a diffraction pattern. Although it is quite simple to go from the image to a convergent-beam diffraction pattern or from an image to a selected-area diffraction pattern (and back), I have found it useful to be able to go between image and diffraction pattern even more quickly. In the method described, once the microscope is set up, it is possible to go from image to diffraction pattern and back by turning just one knob. This makes many operations on the microscope much more convenient. It should be made clear that, in this method, neither the image nor the diffraction pattern is “ideal” (details below), but both are good enough for many necessary procedures.

Electron Image Series Reconstruction of Twin Interfaces in InP Superlattice Nanowires

2011 ◽  
Vol 17 (5) ◽  
pp. 752-758 ◽  
Author(s):  
Martin Ek ◽  
Magnus T. Borgström ◽  
Lisa S. Karlsson ◽  
Crispin J.D. Hetherington ◽  
L. Reine Wallenberg
Keyword(s):  
Electron Microscope ◽  
Diffraction Pattern ◽  
Transmission Electron Microscope ◽  
Interface Structure ◽  
Twin Structure ◽  
Twin Interface ◽  
Transmission Electron ◽  
Electron Image ◽  
Model Structures ◽  
Aberration Corrected

AbstractThe twin interface structure in twinning superlattice InP nanowires with zincblende structure has been investigated using electron exit wavefunction restoration from focal series images recorded on an aberration-corrected transmission electron microscope. By comparing the exit wavefunction phase with simulations from model structures, it was possible to determine the twin structure to be the ortho type with preserved In-P bonding order across the interface. The bending of the thin nanowires away from the intended ⟨110⟩ axis could be estimated locally from the calculated diffraction pattern, and this parameter was successfully taken into account in the simulations.

Aberrations in the Transmission Electron Microscope

1970 ◽  
Vol 28 ◽  
pp. 352-353
Author(s):  
R.A. Ploc
Keyword(s):  
Electron Microscope ◽  
Transmission Electron Microscope ◽  
Landau Equation ◽  
Image Resolution ◽  
Bethe Equation ◽  
Aperture Size ◽  
Resolution Limit ◽  
Transmission Electron ◽  
The Mean ◽  
Pass Through

Three aberrations contribute to the loss of image resolution in the transmission electron microscope; spherical (SA=Csα3), chromatic (CA=Ccα△VV-1) and diffraction (DA=O.61ƛα-1). For high voltage incident electrons and thin materials most microscopists assume resolution is controlled by spherical and diffraction aberrations. We shall discuss whether equating the SA and DA to derive an optimum aperture size (related to αo) and resolution limit (1) is a valid procedure.To determine △V for a given material requires the use of either the Bethe or Landau equations. The Landau formula can be used to give the width of the energy spectrum and the Bethe equation, the mean energy loss after the incident electrons pass through the foil. Since the former is the most probable quantity contributing to CA, Figures 1 and 2 are based on the use of the Landau equation. Zirconium of thickness, t, will be considered for the accelerating voltages 105 and 106 eV.

Determine the Three-Dimensional Crystallographic Misorientation in Heterostructures by Selected Area Diffraction (SAD) in TEM

2000 ◽  
Vol 618 ◽  
Author(s):  
X. J. Guo ◽  
C.-Y. Wen ◽  
J. H. Huang ◽  
H. C. Shih
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
Diffraction Pattern ◽  
Transmission Electron Microscope ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Heteroepitaxial Structures ◽  
Transmission Electron ◽  
Microscope Images ◽  
Diffraction Patterns

ABSTRACTWe proposed a concise and novel scheme to determine the crystallographic misorientation of heteroepitaxial structures. In addition to subtle high-resolution transmission electron microscope images, the information revealed from selected-area diffraction patterns at the interfaces offers another path to determine the angles of misorientations. The principle is to extract the basically three-dimensional misorientation information from a two-dimensional selected-area diffraction pattern through the employment of the Laue circle

scholarly journals Observation of the Transport and Removal of Lipofuscin from the Mouse Myocardium using Transmission Electron Microscope

2020 ◽  
Author(s):  
Lei Wang ◽  
Chang-Yi Xiao ◽  
Jia-Hua Li ◽  
Gui-Cheng Tang ◽  
Shuo-Shuang Xiao
Keyword(s):  
Endothelial Cells ◽  
Electron Microscope ◽  
Blood Vessel ◽  
Transmission Electron Microscope ◽  
Fine Particles ◽  
Transmission Electron ◽  
Thin Sectioning ◽  
Capillary Endothelial Cells ◽  
Lipofuscin Granules ◽  
Pass Through

AbstractThis study was performed to investigate whether the lipofuscin formed within cardiomyocytes can be excluded by the myocardial tissue. We have provided indicators that can be used for future studies on anti-aging interventions.In the present study, the heart of a 5-month-old BALB/c mouse was obtained for resin embedding and ultra-thin sectioning. The specimens were observed under a Hitach 7500 transmission electron microscope, and the images were acquired using an XR401 side-insertion device.Lipofuscin granules are found abundantly in myocardial cells. Cardiomyocytes can excrete lipofuscin granules into the myocardial interstitium using capsule-like protrusions that are formed on the sarcolemma. These granules enter the myocardial interstitium and can be de-aggregated to form “membrane-like garbage”, which can pass from the myocardial stroma into the lumen of the vessel through its walls in the form of soluble fine particles through diffusion or endocytosis of capillaries. Smaller lipofuscin granules can pass through the walls of the vessels and enter the blood vessel lumen through the active transport function of the capillary endothelial cells. When the extended cytoplasmic end of macrophages and fibroblasts fuse with the endothelial cells, the lipofuscin granules or clumps found in the cells of the myocardial interstitium are transported to the capillary walls, and then, they are released into the lumen of the blood vessel by the endothelial cells.The myocardial tissues of mice have the ability to eliminate the lipofuscin produced in the cardiomyocytes into the myocardial blood circulation. Although there are several mechanisms through which the myocardial tissues release lipofuscin into the bloodstream, it is mainly carried out in the form of small, fine, soluble, continuous transport.

Electron Beam Induced Oxygen Disordering in Yba2cu307-X Superconductors

1989 ◽  
Vol 157 ◽  
Author(s):  
S. N. Basu ◽  
T. Roy ◽  
T. E. Mitchell ◽  
M. Nastasi
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Dose Rate ◽  
Diffraction Pattern ◽  
Transmission Electron Microscope ◽  
Electron Irradiation ◽  
Prolonged Irradiation ◽  
Thin Foils ◽  
Transmission Electron ◽  
Ybco Superconductors

ABSTRACTThin foils of bulk YBa2Cu3O7–x (YBCO) superconductors were subjected to electron irradiation in a Transmission Electron Microscope (TEM). The resulting disordering of the oxygen atoms and vacancies in the Cu-0 planes was monitored by measuring the splitting of the (110) diffraction spots in the [001] diffraction pattern. Samples were irradiated at 83K with 100, 150, 200 and 300kV electrons. The 100kV electrons did not cause any disordering, even after prolonged irradiation. The results of the higher energy irradiations showed an excellent fit to a disordering model, indicating a lack of radiation assisted reordering at 83K. This was further confirmed by the insensitivity of the disordering to the dose rate of 300kV electrons at 83K. However, at 300K, an increase in the dose rate of 300kV electrons increased the disordering rate, indicating that radiation assisted reordering was occuring at that temperature.

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

Surface Structure of the Spores of Glugea Weissenbergi

1969 ◽  
Vol 27 ◽  
pp. 238-239
Author(s):  
Sanford H. Vernick ◽  
Anastasios Tousimis ◽  
Victor Sprague
Keyword(s):  
Electron Microscope ◽  
Surface Structure ◽  
Transmission Electron Microscope ◽  
Mature Spore ◽  
Spore Surface ◽  
Sectioned Material ◽  
Transmission Electron ◽  
Surface Detail

Recent electron microscope studies have greatly expanded our knowledge of the structure of the Microsporida, particularly of the developing and mature spore. Since these studies involved mainly sectioned material, they have revealed much internal detail of the spores but relatively little surface detail. This report concerns observations on the spore surface by means of the transmission electron microscope.

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