In-Gel Microwave-Assisted Acid Hydrolysis of Proteins Combined with Liquid Chromatography Tandem Mass Spectrometry for Mapping Protein Sequences

2013 ◽  
Vol 86 (1) ◽  
pp. 600-607 ◽  
Author(s):  
Difei Sun ◽  
Nan Wang ◽  
Liang Li
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Acid Hydrolysis ◽  
Protein Sequences ◽  
Tandem Mass ◽  
Microwave Assisted ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Hydrolysis Of

Tylosin Detection in Animal Feed by Liquid Chromatography−Tandem Mass Spectrometry with Enzymatic Hydrolysis of the Tylosin Urea Adduct

2004 ◽  
Vol 52 (10) ◽  
pp. 2803-2806 ◽  
Author(s):  
Christof Van Poucke ◽  
Fréderic Dumoulin ◽  
Kirsten De Keyser ◽  
Chris Elliott ◽  
Carlos Van Peteghem
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Enzymatic Hydrolysis ◽  
Tandem Mass Spectrometry ◽  
Animal Feed ◽  
Tandem Mass ◽  
Urea Adduct ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Hydrolysis Of

scholarly journals Liquid Chromatography–Tandem Mass Spectrometry Analysis of Erythrocyte Thiopurine Nucleotides and Effect of Thiopurine Methyltransferase Gene Variants on These Metabolites in Patients Receiving Azathioprine/6-Mercaptopurine Therapy

2005 ◽  
Vol 51 (11) ◽  
pp. 2074-2084 ◽  
Author(s):  
Thierry Dervieux ◽  
Gary Meyer ◽  
Robert Barham ◽  
Mariko Matsutani ◽  
Mary Barry ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Acid Hydrolysis ◽  
Perchloric Acid ◽  
Mass Spectrometry Analysis ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Increased Risk ◽  
Liquid Chromatography Tandem Mass

Abstract Background: Polymorphic thiopurine S-methyltransferase (TPMT) is a major determinant of thiopurine toxicity. Methods: We extracted 6-thioguanine nucleotides (6-TGNs) and 6-methylmercaptopurine nucleotides (6-MMPNs) from erythrocytes with perchloric acid and converted them to 6-thioguanine (6-TG) and a 6-methylmercaptopurine (6-MMP) derivative during a 60-min acid hydrolysis step. The liquid chromatography system consisted of a C18 column with an ammonium acetate–formic acid–acetonitrile buffer. 8-Bromoadenine was the internal standard. Analytes were measured with positive ionization and multiple reaction monitoring mode. With PCR–restriction fragment length polymorphism analysis and TaqMan allelic discrimination, common TPMT alleles (*1, *2, *3A, *3B, *3C) were determined in 31 792 individuals. We used perchloric acid extraction, acid hydrolysis, and HPLC with ultraviolet detection to measure erythrocyte 6-TG and 6-MMP nucleotide concentrations in 6189 patients with inflammatory bowel disease receiving azathioprine/6-mercaptopurine therapy. Results: Intra- and interday imprecision were <10% at low and high analyte concentrations. The conversion of 6-TG and 6-MMP nucleoside mono-, di-, and triphosphates was complete after hydrolysis. Allelic frequency for TPMT variant alleles ranged from 0.0063% (*3B) to 3.61% (*3A). Compared with wild types, TPMT heterozygotes had an 8.3-fold higher risk for 6-TGNs >450 pmol/8 × 108 erythrocytes (concentration associated with increased risk for leukopenia), but an 8.2-fold lower risk for 6-MMPNs >5700 pmol/8 × 108 erythrocytes (concentration associated with increased risk for hepatotoxicity). Conclusions: The liquid chromatography–tandem mass spectrometry method can be applied to the routine monitoring of thiopurine therapy. The association between TPMT genotype and metabolite concentrations illustrates the utility of pharmacogenetics in the management of patients undergoing treatment with thiopurines.

Identification of the xanthone of the capitulae from Syngonatnhus nitens (Eriocaulaceae) by liquid chromatography tandem mass spectrometry

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
M Pacifico ◽  
F Hilario ◽  
A Napolitano ◽  
C Pizza ◽  
S Piacente ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass
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