Isothermal Nucleic Acid Amplification Strategy by Cyclic Enzymatic Repairing for Highly Sensitive MicroRNA Detection

2014 ◽  
Vol 86 (14) ◽  
pp. 6763-6767 ◽  
Author(s):  
Dian-Ming Zhou ◽  
Wen-Fang Du ◽  
Qiang Xi ◽  
Jia Ge ◽  
Jian-Hui Jiang
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Amplification Strategy

An ATP-fueled nucleic acid signal amplification strategy for highly sensitive microRNA detection

2018 ◽  
Vol 54 (77) ◽  
pp. 10897-10900 ◽  
Author(s):  
Zhi-Bin Wen ◽  
Wen-Bin Liang ◽  
Ying Zhuo ◽  
Cheng-Yi Xiong ◽  
Ying-Ning Zheng ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Nucleic Acid ◽  
Signal Amplification ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Strand Displacement ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21.

scholarly journals Highly sensitive dual mode electrochemical platform for microRNA detection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pawan Jolly ◽  
Marina R. Batistuti ◽  
Anna Miodek ◽  
Pavel Zhurauski ◽  
Marcelo Mulato ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Dual Mode ◽  
Electrode Surfaces ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Detection Mode ◽  
Amplification Strategy

Abstract MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of microRNAs. The platform was developed using peptide nucleic acids as probes on gold electrode surfaces to capture target miRNAs. A simple amplification strategy using gold nanoparticles has been employed exploiting the inherent charges of the nucleic acids. Electrochemical impedance spectroscopy was used to monitor the changes in capacitance upon any binding event, without the need for any redox markers. By using thiolated ferrocene, a complementary detection mode on the same sensor was developed where the increasing peaks of ferrocene were recorded using square wave voltammetry with increasing miRNA concentration. This dual-mode approach allows detection of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatched target miRNA sequences. The electrochemical platform developed can be easily expanded to other miRNA/DNA detection along with the development of microarray platforms.

Electricity-free chemical heater for isothermal nucleic acid amplification with applications in COVID-19 home testing

The Analyst ◽  
2021 ◽  
Author(s):  
Rui Jie Li ◽  
Michael G. Mauk ◽  
Youngung Seok ◽  
Haim H. Bau
Keyword(s):  
Nucleic Acid ◽  
Phase Change ◽  
Phase Change Material ◽  
Heat Generation ◽  
Temperature Regulation ◽  
Nucleic Acid Amplification ◽  
Enzymatic Amplification ◽  
Isothermal Nucleic Acid Amplification ◽  
Home Testing ◽  
Change Material

Electricty-free incubation of isothermal enzymatic amplification with a composite comprised of exothermic reactants for heat generation and phase change material for temperature regulation.

scholarly journals Rapid electrostatic DNA enrichment for sensitive detection of Trichomonas vaginalis in clinical urinary samples

2020 ◽  
Vol 12 (8) ◽  
pp. 1085-1093
Author(s):  
Justin M. Rosenbohm ◽  
James M. Robson ◽  
Rishabh Singh ◽  
Rose Lee ◽  
Jane Y. Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Trichomonas Vaginalis ◽  
Nucleic Acid Amplification ◽  
Urine Samples ◽  
Sensitive Detection ◽  
Isothermal Nucleic Acid Amplification

Rapid electrostatic enrichment of DNA from urine samples for improved isothermal nucleic acid amplification-based detection of Trichomonas vaginalis.

scholarly journals Interfacial Effect-Based Quantification of Droplet Isothermal Nucleic Acid Amplification for Bacterial Infection

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Tiffany-Heather Ulep ◽  
Alexander S. Day ◽  
Katelyn Sosnowski ◽  
Alexa Shumaker ◽  
Jeong-Yeol Yoon
Keyword(s):  
Nucleic Acid ◽  
Bacterial Infection ◽  
Nucleic Acid Amplification ◽  
Interfacial Effect ◽  
Isothermal Nucleic Acid Amplification
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