scholarly journals Highly sensitive dual mode electrochemical platform for microRNA detection

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Pawan Jolly ◽  
Marina R. Batistuti ◽  
Anna Miodek ◽  
Pavel Zhurauski ◽  
Marcelo Mulato ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Dual Mode ◽  
Electrode Surfaces ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Detection Mode ◽  
Amplification Strategy

Abstract MicroRNAs (miRNAs) play crucial regulatory roles in various human diseases including cancer, making them promising biomarkers. However, given the low levels of miRNAs present in blood, their use as cancer biomarkers requires the development of simple and effective analytical methods. Herein, we report the development of a highly sensitive dual mode electrochemical platform for the detection of microRNAs. The platform was developed using peptide nucleic acids as probes on gold electrode surfaces to capture target miRNAs. A simple amplification strategy using gold nanoparticles has been employed exploiting the inherent charges of the nucleic acids. Electrochemical impedance spectroscopy was used to monitor the changes in capacitance upon any binding event, without the need for any redox markers. By using thiolated ferrocene, a complementary detection mode on the same sensor was developed where the increasing peaks of ferrocene were recorded using square wave voltammetry with increasing miRNA concentration. This dual-mode approach allows detection of miRNA with a limit of detection of 0.37 fM and a wide dynamic range from 1 fM to 100 nM along with clear distinction from mismatched target miRNA sequences. The electrochemical platform developed can be easily expanded to other miRNA/DNA detection along with the development of microarray platforms.

scholarly journals Electrochemical Immunosensors Based on Screen-Printed Gold and Glassy Carbon Electrodes: Comparison of Performance for Respiratory Syncytial Virus Detection

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 175
Author(s):  
Wioleta Białobrzeska ◽  
Daniel Firganek ◽  
Maciej Czerkies ◽  
Tomasz Lipniacki ◽  
Marta Skwarecka ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Glassy Carbon ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Electrochemical Characterizations ◽  
Virus Detection ◽  
Electrochemical Immunosensors ◽  
Syncytial Virus ◽  
Screen Printed

This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 × 105 PFU/mL to 1.5×107 PFU/mL, more than 1.0 × 105 PFU/mL to 1.0 × 107 PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 × 103 PFU/mL, three orders of magnitude lower than 2.85 × 106 PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility.

scholarly journals Electrochemical Sensing of Idarubicin—DNA Interaction Using Electropolymerized Azure B and Methylene Blue Mediation

Chemosensors ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 33
Author(s):  
Anastasia Goida ◽  
Yurii Kuzin ◽  
Vladimir Evtugyn ◽  
Anna Porfireva ◽  
Gennady Evtugyn ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Biological Fluids ◽  
Dna Interaction ◽  
Electrochemical Sensing ◽  
Dna Sensor ◽  
Azure B ◽  
Fish Sperm ◽  
Highly Sensitive

A highly sensitive electrochemical DNA sensor for detection of the chemotherapeutic drug idarubicin mediated by Methylene blue (MB) has been developed. DNA from fish sperm has been immobilized at the electropolymerized layers of Azure B. The incorporation of MB into the DNA layers substantially increased the sensor sensitivity. The concentration range for idarubicin determination by cyclic voltammetry was from 1 fM to 0.1 nM, with a limit of detection (LOD) of 0.3 fM. Electrochemical impedance spectroscopy (EIS) in the presence of a redox probe ([Fe(CN)6]3−/4−) allowed for the widening of a linear range of idarubicin detection from 1 fM to 100 nM, retaining LOD 0.3 fM. The DNA sensor has been tested in various real and artificial biological fluids with good recovery ranging between 90–110%. The sensor has been successfully used for impedimetric idarubicin detection in medical preparation Zavedos®. The developed DNA biosensor could be useful for the control of the level of idarubicin during cancer therapy as well as for pharmacokinetics studies.

scholarly journals A dumbbell probe-mediated rolling circle amplification strategy for highly sensitive microRNA detection

2010 ◽  
Vol 38 (15) ◽  
pp. e156-e156 ◽  
Author(s):  
Yuntao Zhou ◽  
Qing Huang ◽  
Jimin Gao ◽  
Jianxin Lu ◽  
Xizhong Shen ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Amplification Strategy

Highly sensitive detection of nucleic acids using a cascade amplification strategy based on exonuclease III-assisted target recycling and conjugated polyelectrolytes

The Analyst ◽  
2018 ◽  
Vol 143 (18) ◽  
pp. 4267-4272 ◽  
Author(s):  
Biqing Bao ◽  
Yanrui Pan ◽  
Bingbing Gu ◽  
Jia Chen ◽  
Yu Xu ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Signal Amplification ◽  
Conjugated Polyelectrolytes ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Exo Iii ◽  
Highly Sensitive Detection ◽  
Amplification Strategy ◽  
Cascade Amplification

A ratiometric and cascade amplification strategy that combines the signal amplification and effecitive FRET property of CPEs with the Exo III-assisted target recycling method has been developed for DNA detection.

WEARABLE SWEAT SENSING DEVICE FOR DETECTION OF IBD BIOMARKERS

2021 ◽  
Vol 27 (Supplement_1) ◽  
pp. S12-S12
Author(s):  
Badrinath Jagannath ◽  
Sriram Muthukumar ◽  
Shalini Prasad
Keyword(s):  
Real Time ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Human Subjects ◽  
The United States ◽  
Healthy Human ◽  
Affinity Capture ◽  
Specific Affinity ◽  
Impedance Spectroscopy Technique

Abstract Introduction Inflammatory Bowel Disease affects 1.2 million in the United States. Flare-up of the disease occurs in a random way and current testing methods lack ability for real-time prediction of a flare up. The levels of cytokines elevate during a flareup. Therefore, we hypothesize that real-time monitoring of cytokine biomarkers can be useful for early detection of flare-ups and provide better patient management. In this context, sweat-based diagnostics can be promising for real-time tracking of IBD biomarkers. Materials and Methods A wearable SWEATSENSER was developed by functionalization of specific affinity capture probes (IL-1β, CRP antibodies) on metal/semiconducting interface deposited on a porous patch substrate. Electrochemical impedance spectroscopy technique was used to detect the interaction between the specific antibody and target analyte. The developed SWEATSENSER was tested on 20 healthy human subjects in compliance with an approved IRB at UT Dallas. Continuous on-body measurements were recorded to report IL-1β, CRP levels in sweat in real-time. Results In this work, a wearable multiplexed sweat sensor for detection of IL-1β, CRP in sweat has been demonstrated. The sensor demonstrates a limit of detection of 1 pg/mL with a dynamic range from 1 pg/mL- 512 pg/mL for both the biomarkers in sweat. The sweat sensor demonstrated excellent correlation with reference ELISA method (Pearson’s r ≥0.95). On-body monitoring using sweat sensor from passively perspired human sweat demonstrated a mean concentration of 28 pg/mL for IL-1β in healthy cohort. Conclusion A wearable sweat sensor was developed to monitor potential IBD markers in sweat. The developed device can be useful in better management of IBD patients.

HIGHLY SENSITIVE METHODS FOR MICRORNA DETECTION USING ISOTHERMAL METHODS FOR NUCLEIC ACIDS AMPLIFICATION

2021 ◽  
Vol 1 (19) ◽  
pp. 244-246
Author(s):  
O.L. Bodulev ◽  
A.M. Soloviev
Keyword(s):  
Nucleic Acids ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Catalytic Hairpin Assembly

This work will present the results of the development of two new highly sensitive heterogeneous methods for the determination of miRNA-141 and miRNA-39 using the isothermal circular strand-displacement amplification and the catalytic hairpin assembly methods for analyte amplification.

Companion and Point-of-Care Sensor System for Rapid Multiplexed Detection of a Panel of Infectious Disease Markers

2017 ◽  
pp. 247263031769677
Author(s):  
Anjan Panneer Selvam ◽  
Shalini Prasad
Keyword(s):  
Whole Blood ◽  
Dynamic Range ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Lipoteichoic Acid ◽  
Impedance Change ◽  
Label Free ◽  
Nylon Membrane ◽  
Multiplexed Detection

A nanochannel-based electrochemical biosensor has been demonstrated for rapid and multiplexed detection of a panel of three biomarkers associated with rapid detection of sepsis. The label-free biosensor detected procalcitonin (PCT), lipoteichoic acid (LTA), and lipopolysaccharide (LPS) from human whole blood. The biosensor comprises a nanoporous nylon membrane integrated onto a microelectrode sensor platform for nanoconfinement effects. Charge perturbations due to biomarker binding are recorded as impedance changes using electrochemical impedance spectroscopy. The measured impedance change is used to quantitatively determine the concentration of the three biomarkers using antibody receptors from the tested sample. We were successful in detecting and quantifying the three biomarkers from whole blood. The limit of detection was 0.1 ng/mL for PCT and 1 µg/mL for LPS and LTA. The sensor was able to demonstrate a dynamic range of detection from 01.1 ng/mL to 10 µg/mL for PCT and from 1 µg/mL to 1000 µg/mL for LPS and LTA biomarkers. This novel technology has promising preliminary results toward the design of sensors for rapid and sensitive detection of the three panel biomarkers in whole blood toward diagnosis and classification of sepsis.

scholarly journals Erratum: Corrigendum: Highly sensitive dual mode electrochemical platform for microRNA detection

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Pawan Jolly ◽  
Marina R. Batistuti ◽  
Anna Miodek ◽  
Pavel Zhurauski ◽  
Marcelo Mulato ◽  
...  
Keyword(s):  
Dual Mode ◽  
Microrna Detection ◽  
Highly Sensitive
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