scholarly journals Probing Protein Denaturation during Size-Exclusion Chromatography Using Native Mass Spectrometry

2020 ◽  
Vol 92 (6) ◽  
pp. 4292-4300 ◽  
Author(s):  
Iro K. Ventouri ◽  
Daniel B. A. Malheiro ◽  
Robert L. C. Voeten ◽  
Sander Kok ◽  
Maarten Honing ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Size Exclusion Chromatography ◽  
Protein Denaturation ◽  
Native Mass Spectrometry ◽  
Size Exclusion ◽  
Exclusion Chromatography

scholarly journals Characterization of trimethoprim resistant E. coli dihydrofolate reductase mutants by mass spectrometry and inhibition by propargyl-linked antifolates

2017 ◽  
Vol 8 (5) ◽  
pp. 4062-4072 ◽  
Author(s):  
Michael Cammarata ◽  
Ross Thyer ◽  
Michael Lombardo ◽  
Amy Anderson ◽  
Dennis Wright ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Size Exclusion Chromatography ◽  
Dihydrofolate Reductase ◽  
Native Mass Spectrometry ◽  
Size Exclusion ◽  
E Coli ◽  
Exclusion Chromatography

Native mass spectrometry, size exclusion chromatography, and kinetic assays were employed to study trimethoprim resistance in E. coli caused by mutations P21L and W30R of dihydrofolate reductase.

scholarly journals Toward Automation of Collision-Induced Unfolding Experiments through Online Size Exclusion Chromatography Coupled to Native Mass Spectrometry

2020 ◽  
Vol 92 (19) ◽  
pp. 12900-12908 ◽  
Author(s):  
Evolène Deslignière ◽  
Anthony Ehkirch ◽  
Thomas Botzanowski ◽  
Alain Beck ◽  
Oscar Hernandez-Alba ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Size Exclusion Chromatography ◽  
Native Mass Spectrometry ◽  
Size Exclusion ◽  
Collision Induced Unfolding ◽  
Exclusion Chromatography

scholarly journals Rapid characterization of biotherapeutic proteins by size-exclusion chromatography coupled to native mass spectrometry

mAbs ◽  
2015 ◽  
Vol 8 (2) ◽  
pp. 331-339 ◽  
Author(s):  
Markus Haberger ◽  
Michael Leiss ◽  
Anna-Katharina Heidenreich ◽  
Oxana Pester ◽  
Georg Hafenmair ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Size Exclusion Chromatography ◽  
Native Mass Spectrometry ◽  
Size Exclusion ◽  
Exclusion Chromatography ◽  
Rapid Characterization
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