Self-Driven Pretreatment and Room-Temperature Storage of Water Samples for Virus Detection Using Enhanced Porous Superabsorbent Polymer Beads

Abstract The continuous emergence of infectious viral diseases has become a major threat to public health. To quantify viruses, proper handling of water samples is required to ensure the accuracy and reliability of the testing results. In this study, we develop enhanced porous superabsorbent polymer (PSAP) beads to pretreat and store water samples for virus detection. By applying PSAP beads to collect water samples, the viruses are captured and encapsulated inside the beads while undesired components are excluded. We have successfully demonstrated that the shelf life of the model virus can be effectively extended at room temperature (22°C) and elevated temperature (35°C). Both the infectivity level and genome abundance of the viruses are protected even in a complex medium like untreated wastewater. Under the tested conditions, the viral degradation rate constant can be reduced to more than 10 times using the PSAP beads. Therefore, the enhanced PSAP beads provide a low-cost and efficient sample pretreatment and storage method that is feasible and practicable for large-scale surveillance of viral pathogens in water samples.

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Comparison of Respiratory Virus Detection Rates for Infants and Toddlers by Use of Flocked Swabs, Saline Aspirates, and Saline Aspirates Mixed in Universal Transport Medium for Room Temperature Storage and Shipping

Journal of Clinical Microbiology ◽

10.1128/jcm.00714-08 ◽

2008 ◽

Vol 46 (7) ◽

pp. 2374-2376 ◽

Cited By ~ 35

Author(s):

P. Walsh ◽

C. L. Overmyer ◽

K. Pham ◽

S. Michaelson ◽

L. Gofman ◽

...

Keyword(s):

Room Temperature ◽

Respiratory Virus ◽

Virus Detection ◽

Infants And Toddlers ◽

Detection Rates ◽

Room Temperature Storage ◽

Transport Medium ◽

Temperature Storage

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Accelerated Aging of Deacidified and Untreated Book Paper in 1967 Compared with 52 Years of Natural Aging

Restaurator International Journal for the Preservation of Library and Archival Material ◽

10.1515/res-2020-0006 ◽

2020 ◽

Vol 41 (3) ◽

pp. 131-152

Author(s):

Tali H. Horst ◽

Richard D. Smith ◽

Antje Potthast ◽

Martin A. Hubbe

Keyword(s):

Room Temperature ◽

Accelerated Aging ◽

Natural Aging ◽

Scanning Electron Micrographs ◽

Surface Ph ◽

Room Temperature Storage ◽

Folding Endurance ◽

Endurance Tests ◽

Breaking Length ◽

Temperature Storage

AbstractThree copies of a book that had been optionally deacidified using two different procedures in 1967, and then subjected to accelerated aging, were tested again after 52 years of natural aging. Matched copies of the book Cooking the Greek Way, which had been printed in Czechoslovakia on acidic paper, were evaluated. Nonaqueous treatment of two of the copies with magnesium methoxide dissolved in chlorofluorocarbon solvent had been found in 1967 to have decreased the susceptibility to embrittlement, as evidenced by the results of the accelerated aging, followed by folding endurance tests. Retesting of the same books in 2019, after 52 years of room temperature storage, showed that the deacidification treatments had achieved the following benefits in comparison to the untreated book: (a) higher brightness; (b) higher folding endurance; (c) tensile breaking length higher in the cross-direction of the paper; (d) substantial alkaline reserve content, (e) an alkaline surface pH in the range 7.1–7.4, and (f) higher molecular mass of the cellulose. Remarkably, some of the folding endurance results matched those of unaged samples evaluated in 1967. Scanning electron micrographs showed no differences between the treated and untreated books.

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The Effect Of Storage On Platelet Morpholog

10.1055/s-0038-1653241 ◽

1981 ◽

Author(s):

A Sturk ◽

L M Burt ◽

T Hakvoort ◽

J W ten cate ◽

N Crawford

Keyword(s):

Electron Microscopy ◽

Membrane Structure ◽

Room Temperature ◽

Surface Membrane ◽

Platelet Concentrates ◽

Room Temperature Storage ◽

Short Survival ◽

Transmission Electron ◽

Temperature Storage ◽

Morphological Correlation

Platelet concentrates were stored for one, two or three days at 4°C (unagitated) or room temperature (unagitated and linearly agitated). The morphology of platelets in platelet concentrates, directly after twice washing at room temperature and after 60 min incubation of the washed platelets at 37°C was investigated by both scanning and transmission electron microscopy.Platelets in the freshly prepared concentrates are slightly activated, i.e. show some pseudopod formation. At 4°C platelets rapidly loose their discoid shape. After three days their surface membrane shows extensive folding, they are slightly vacuolated and have lost most of their granules. Incubation of these cold-stored platelets at 37°C does not induce reversal to the discoid shape.Room temperature storage results in reversal of the slight initial platelet activation. After three days unagitated platelets are slightly more vacuolated than platelets stored with agitation. Room temperature storage usually results in remarkably well preserved, discoid platelets. Occasionally however, agitated platelet concentrates contain a high proportion of odd shaped cells. As platelets stored at 4°C did not became discoid after incubation at 37°, the altered membrane structure could provide an explanation for their short survival upon transfusion. Our results also provide a morphological correlation with the slightly better recovery and survival of platelets stored agitated vs.- non-agitated platelets at room temperature.

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The effects of nanoscale geometry and spillover on room temperature storage of hydrogen on silica nanosprings

Journal of Physics D Applied Physics ◽

10.1088/0022-3727/46/50/505307 ◽

2013 ◽

Vol 46 (50) ◽

pp. 505307 ◽

Cited By ~ 4

Author(s):

Giancarlo Corti ◽

Yingqian Zhan ◽

Lidong Wang ◽

Brian Hare ◽

Timothy Cantrell ◽

...

Keyword(s):

Room Temperature ◽

Room Temperature Storage ◽

Silica Nanosprings ◽

Temperature Storage

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Mineral Oil-Chitosan Emulsion Coatings Affect Quality and Shelf-Life of Coated Eggs during Refrigerated and Room Temperature Storage

Journal of Food Science ◽

10.1111/j.1750-3841.2011.02125.x ◽

2011 ◽

Vol 76 (4) ◽

pp. S262-S268 ◽

Cited By ~ 25

Author(s):

Damir D. Torrico ◽

Hong Kyoon No ◽

Witoon Prinyawiwatkul ◽

Marlene Janes ◽

José A.H. Corredor ◽

...

Keyword(s):

Shelf Life ◽

Room Temperature ◽

Mineral Oil ◽

Room Temperature Storage ◽

Quality And Shelf Life ◽

Temperature Storage

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Evaluasi Penyimpanan Spermatozoa Ayam Pada Suhu 5⁰C, 26⁰C Dengan Pengencer Infuse NaCl, Glukosa 5% dan 10%

Jurnal Sains Teknologi & Lingkungan ◽

10.29303/jstl.v0i0.242 ◽

2021 ◽

pp. 10-19

Author(s):

Asnawi Asnawi ◽

Maskur Maskur ◽

Adji Santoso Dradjat

Keyword(s):

Cold Storage ◽

Room Temperature ◽

Storage Temperature ◽

Room Temperature Storage ◽

C Storage ◽

Better Than ◽

Temperature Storage

The purpose of this study were to compare the quality of spermatozoa stored at 26⁰C, 5⁰C using diluents of NaCl, 10% glucose and 5% glucose. The spermatozoa of a rooster was collected and divided into 6 parts, each 2 tubes diluted in a ratio of 1:1 using NaCl, Glucose5% and Glucose 10%, then each 3 tubes with different diluents were stored at 26⁰C and 5⁰C. Observations of motility, viability and abnormalities of spermatozoa were carried out half an hour, 1 hour after dilution, followed every 2 hours until the ninth hours. The results showed that spermatozoa stored for 9 hours at a temperature of 26⁰C with a physiological diluent of NaCl, 10% Glucose and 5% Glucose each were different (P, < 0.05) with motility 50 ± 0.0%, 42 ± 10.95. % and 34±8.94%, respectively. At storage temperature of 5⁰C for 9 hours, physiological NaCl, 10% glucose and 5% glucose were significantly different (P<0.05) with motility 58.00±10.95%, 46.00±8.94% and 38.00±, respectively. 10.95% in a row. The viability of spermatozoa at 26⁰C storage with 5% glucose diluent was better than 10% glucose and physiological NaCl (P<0.05), 58.93±1.27%, 42.93±1.48% and 33.43±1.27% , while the physiological NaCl diluent and 10% glucose were not significantly different (P>0.05). At 5⁰C storage the viability of spermatozoa in the three diluents was not significantly different, with values of Glucose 10%, Glucose 5% and physiological NaCl 52.57±5.15%, 52.21±5.02% and 48.14±8.09%, respectively. Spermatozoa abnormalities at storage temperature 26⁰C and 5⁰C for 9 hours using physiological NaCl diluent, 5% glucose and 10% glucose, were not significantly different and varied between 5 to 10%. Finally, it can be concluded that at room temperature storage less than 4 hours the quality of spermatozoa was better with 5% glucose diluent, while for cold storage beyond 4 hours the quality of spermatozoa with NaCl diluent was higher

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