Aplikasi Zeolit-KMnO4 dan Silica Gel untuk Memperpanjang Green Life Mangga Arumanis (Mangifera Indica L)

Mangoes are harvested when ripe have an "eating quality" that consumers are them in, but quickly reach the senescence phase, making it less profitable for businesses. As a climacteric fruit, the ripening process of mango can be slowed down by using an ethylene adsorber. This study aims to determine the combination of zeolite-KMnO4 and silica gel as ethylene adsorber (EAB) to maintain the green life of ripe The material used is ethylene adsorber (EAB) which is applied to mango arumanis which is packaged with a weight package of 1000±50 g. After the shelf life is reached, the EAB is removed from the packaging and the mangoes are left at room temperature for natural ripening and continued until conditions are not acceptable to consumers. The results showed that the EAB application was able to maintain the green life of mangoes by the scenario of the shelf life both at cold and room temperature storage. Natural ripening of mango was achieved 5 days and 2 days after EAB was removed from the packaging, for cold and room temperature storage, respectively. The length of time until the panellists did not receive it was 20 days for cold storage and 12 days for the room, while the control for cold storage was 6 and 3 days at room temperature

A preliminary study of the effects of cold, frozen, or room temperature storage of commercial feeds on growth performance and feed consumption of juvenile rainbow trout (Oncorhynchus mykiss)

The storage of commercial feeds in a cool and dry place is a prerequisite management strategy to minimize the deterioration of commercial feeds. This study investigated the effects of feed storage conditions on feed intake and growth performances of juvenile rainbow trout (Oncorhynchus mykiss). A total of 240 juvenile rainbow trout, weighing 8.7–10.5 g, were randomly distributed into four groups. Each group was further subdivided into three replicates with 20 fish in each. The experimental fish were fed to apparent satiation twice daily over a 35-days period using four commercial feeds previously kept at different storage conditions, i.e., room temperature storage (20.8°C), cold storage (10°C), or frozen storages (-1.1°C and -15°C). The feed utilization was assessed by relative feed intake (RFI%) and feed conversion rate (FCR), while growth performance was evaluated by the thermal-unit growth coefficient (TGC) and specific growth rate (SGR). The preliminary findings suggest that the food storage conditions did not significantly affect feed intake and growth performance in juvenile rainbow trout. The findings provide practical information for fish farmers in the management of feed storage, which covers a remarkable proportion of the total production costs in aquaculture facilities.

Evaluasi Penyimpanan Spermatozoa Ayam Pada Suhu 5⁰C, 26⁰C Dengan Pengencer Infuse NaCl, Glukosa 5% dan 10%

The purpose of this study were to compare the quality of spermatozoa stored at 26⁰C, 5⁰C using diluents of NaCl, 10% glucose and 5% glucose. The spermatozoa of a rooster was collected and divided into 6 parts, each 2 tubes diluted in a ratio of 1:1 using NaCl, Glucose5% and Glucose 10%, then each 3 tubes with different diluents were stored at 26⁰C and 5⁰C. Observations of motility, viability and abnormalities of spermatozoa were carried out half an hour, 1 hour after dilution, followed every 2 hours until the ninth hours. The results showed that spermatozoa stored for 9 hours at a temperature of 26⁰C with a physiological diluent of NaCl, 10% Glucose and 5% Glucose each were different (P, < 0.05) with motility 50 ± 0.0%, 42 ± 10.95. % and 34±8.94%, respectively. At storage temperature of 5⁰C for 9 hours, physiological NaCl, 10% glucose and 5% glucose were significantly different (P<0.05) with motility 58.00±10.95%, 46.00±8.94% and 38.00±, respectively. 10.95% in a row. The viability of spermatozoa at 26⁰C storage with 5% glucose diluent was better than 10% glucose and physiological NaCl (P<0.05), 58.93±1.27%, 42.93±1.48% and 33.43±1.27% , while the physiological NaCl diluent and 10% glucose were not significantly different (P>0.05). At 5⁰C storage the viability of spermatozoa in the three diluents was not significantly different, with values of Glucose 10%, Glucose 5% and physiological NaCl 52.57±5.15%, 52.21±5.02% and 48.14±8.09%, respectively. Spermatozoa abnormalities at storage temperature 26⁰C and 5⁰C for 9 hours using physiological NaCl diluent, 5% glucose and 10% glucose, were not significantly different and varied between 5 to 10%. Finally, it can be concluded that at room temperature storage less than 4 hours the quality of spermatozoa was better with 5% glucose diluent, while for cold storage beyond 4 hours the quality of spermatozoa with NaCl diluent was higher

Pengaruh Ekstrak Propolis sebagai Edible Coating terhadap Karakteristik Kimia dan Aktifitas Antioksidan Daging Sapi pada Penyimpanan Suhu Ruang

This aim of this study is to determine the effect of using propolis extract as edible coating for beefon chemical characteristics consisting of moisture content, malondialdehyde value and antioxidantactivity at room temperature storage. This research used randomized design with 4 treatments and 3replications. The treatment applied in this study was immersing the meat in propolis extract with differentconcentrations, that are 0.5%, 1.0%, and 1.5% propolis coating. Coating meat with the addition ofpropolis extract was able to have a significant effect at a concentration of 0.5% on water content and theantioxidant activity of coating meat (P <0.05). The addition of propolis extract at various concentrationscan maintain the quality of beef by reducing rancidity during storage. The increasing concentration ofpropolis extract caused the decreasing of MDA value.

Room-temperature Storage of Lyophilized Engineered Bacteria using Tardigrade Intrinsically Disordered Proteins

Tardigrades, which live in transiently wet environments such as moss, are well-known for their extreme resistance to desiccation. Tardigrade intrinsically disordered proteins (TDPs) have been reported to also protect bacteria and yeast under desiccation. In this study, we utilized lyophilization to achieve room-temperature storage of engineered bacteria. By using TDPs, engineered bacteria are protected under lyophilization and their original functions are preserved. This study shows that TDPs can be expressed in the Escherichia coli (E. coli) BL21 and DH5α, and bacteria treated with Cytosolic-abundant heat soluble protein (CAHS) 106094 displayed the highest survival rate after lyophilization. Moreover, this study shows that the co-expression of TDPs can improve the preservation of bacteria and maintain high survival rates after prolonged room temperature storage. Additionally, the TDPs can be expressed using different vectors, which means that they can be used in different types of engineered bacteria. This study offers a new storage method that not only improves the storage of biological material for industrial and daily usage, but also for future iGEM (International Genetically Engineered Machine Competition) teams to store and use their engineered bacteria in different applications.