scholarly journals Corection to “Enhanced Nitrogen Removal and Anammox Bacteria Retention with Zeolite-Coated Membrane in Simulated Mainstream Wastewater”

2021 ◽  
Author(s):  
Anndee L. Huff Chester ◽  
Kiwon Eum ◽  
Michael Tsapatsis ◽  
Marc A. Hillmyer ◽  
Paige J. Novak
Keyword(s):  
Nitrogen Removal ◽  
Anammox Bacteria ◽  
Coated Membrane

scholarly journals Temperature modulates stress response in anammox reactors

2020 ◽  
Author(s):  
Robert Niederdorfer ◽  
Damian Hausherr ◽  
Alejandro Palomo ◽  
Jing Wei ◽  
Paul Magyar ◽  
...  
Keyword(s):  
Stress Response ◽  
Dissolved Oxygen ◽  
Nitrogen Removal ◽  
High Stress ◽  
Anammox Bacteria ◽  
Transcriptional Responses ◽  
Microbial Biofilms ◽  
Temperature Regimes ◽  
Anammox Activity ◽  
Stress Susceptibility

AbstractAutotrophic nitrogen removal by anaerobic ammonium oxidizing (anammox) bacteria is an energy-efficient nitrogen removal process in wastewater treatment. However, full-scale deployment under mainstream conditions remains challenging for practitioners due to the high stress susceptibility of anammox bacteria towards fluctuations in dissolved oxygen and temperature. Here, we investigated the response of microbial biofilms with verified anammox activity to oxygen shocks under favorable and cold temperature regimes. Genome-centric metagenomics and metatranscriptomics were used to investigate the stress response on various biological levels. We show that temperature regime and strength of oxygen perturbations induced divergent responses from the process level down to the transcriptional profile of individual taxa. Temperature induced distinct transcriptional states in compositionally identical communities and transient pulses of dissolved oxygen resulted in the upregulation of stress-response only under favorable temperatures. Anammox species and other key biofilm taxa display different transcriptional responses to the induced stress regimes.

scholarly journals Enhanced Nitrogen Removal from Domestic Wastewater by Partial-Denitrification/Anammox in an Anoxic/Oxic Biofilm Reactor

Processes ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 109
Author(s):  
Yu Huang ◽  
Yongzhen Peng ◽  
Donghui Huang ◽  
Jiarui Fan ◽  
Rui Du
Keyword(s):  
Nitrogen Removal ◽  
Oxygen Demand ◽  
Domestic Wastewater ◽  
Biofilm Reactor ◽  
Anammox Bacteria ◽  
Stable Operation ◽  
Ammonium Oxidation ◽  
N Accumulation ◽  
Carbon Demand ◽  
Oxidation Efficiency

A partial-denitrification coupling with anaerobic ammonium oxidation (anammox) process (PD/A) in a continuous-flow anoxic/oxic (A/O) biofilm reactor was developed to treat carbon-limited domestic wastewater (ammonia (NH4+-N) of 55 mg/L and chemical oxygen demand (COD) of 148 mg/L in average) for about 200 days operation. Satisfactory NH4+-N oxidation efficiency above 95% was achieved with rapid biofilm formation in the aerobic zone. Notably, nitrite (NO2−-N) accumulation was observed in the anoxic zone, mainly due to the insufficient electron donor for complete nitrate (NO3−-N) reduction. The nitrate-to-nitrite transformation ratio (NTR) achieved was as high as 64.4%. After the inoculation of anammox-enriched sludge to anoxic zones, total nitrogen (TN) removal was significantly improved from 37.3% to 78.0%. Anammox bacteria were effectively retained in anoxic biofilm utilizing NO2−-N produced via the PD approach and NH4+-N in domestic wastewater, with the relative abundance of 5.83% for stable operation. Anammox pathway contributed to TN removal by a high level of 38%. Overall, this study provided a promising method for mainstream nitrogen removal with low energy consumption and organic carbon demand.

Performance and inhibition recovery of anammox reactors seeded with different types of sludge

2011 ◽  
Vol 63 (4) ◽  
pp. 710-718 ◽  
Author(s):  
S. Q. Ni ◽  
J. Meng
Keyword(s):  
Nitrogen Removal ◽  
Granular Sludge ◽  
Recovery Process ◽  
Nitrogen Loading ◽  
Anaerobic Sludge ◽  
Anammox Bacteria ◽  
Recovery Processes ◽  
Anammox Activity ◽  
Different Types ◽  
Anaerobic Sludge Blanket

In order to study the performance, inhibition and recovery processes of different types of anammox sludge, three up-flow anaerobic sludge blanket reactors were inoculated with flocculent sludge, granular sludge, and cultured inactive methanogenic granules. During stable period, with nitrogen loading rates of 0.9–1.1 kg/m3/d, the total nitrogen removal efficiencies of these reactors averaged at 86.5%, 90.8% and 93.5%, respectively. The kinetics study indicated that the reactor seeded with cultured inactive methanogenic granules possessed the highest nitrogen removal potential, followed by the granular anammox reactor and the flocculent anammox reactor. The study suggested that a concentration as high as 988.3 mg NH4+-N/L and 484.4 mg NO2−-N/L could totally inhibit granular anammox bacteria and result in a inhibition of 50% flocculent anammox activity. In addition, reactors seeded with flocculent sludge and anammox granules could be fully recovered by decreasing their influent substrate concentrations. However, the decrease of influent substrate concentration for the reactor with cultured inactive methanogenic granules could only restore about 75% of its bacterial activity. In this study, anammox bacteria purity was the major factor to evaluate the recovery ability in comparison with sludge type. Free ammonia was a more appropriate indicator for the anammox recovery process compared to free nitric acid.

High growth potential and nitrogen removal performance of marine anammox bacteria in shrimp-aquaculture sediment

Chemosphere ◽  
2018 ◽  
Vol 196 ◽  
pp. 69-77 ◽  
Author(s):  
Luong Van Duc ◽  
Bongkeun Song ◽  
Hiroaki Ito ◽  
Takehide Hama ◽  
Masashi Otani ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
High Growth ◽  
Growth Potential ◽  
Shrimp Aquaculture ◽  
Anammox Bacteria ◽  
High Growth Potential

scholarly journals 454-Pyrosequencing Analysis of Bacterial Communities from Autotrophic Nitrogen Removal Bioreactors Utilizing Universal Primers: Effect of Annealing Temperature

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Alejandro Gonzalez-Martinez ◽  
Alejandro Rodriguez-Sanchez ◽  
Belén Rodelas ◽  
Ben A. Abbas ◽  
Maria Victoria Martinez-Toledo ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Nitrogen Removal ◽  
Annealing Temperature ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Universal Primers ◽  
Anammox Bacteria ◽  
Universal Primer ◽  
Optimum Annealing Temperature ◽  
Autotrophic Nitrogen Removal

Identification of anaerobic ammonium oxidizing (anammox) bacteria by molecular tools aimed at the evaluation of bacterial diversity in autotrophic nitrogen removal systems is limited by the difficulty to design universal primers for theBacteriadomain able to amplify the anammox 16S rRNA genes. A metagenomic analysis (pyrosequencing) of total bacterial diversity including anammox population in five autotrophic nitrogen removal technologies, two bench-scale models (MBR and Low Temperature CANON) and three full-scale bioreactors (anammox, CANON, and DEMON), was successfully carried out by optimization of primer selection and PCR conditions (annealing temperature). The universal primer 530F was identified as the best candidate for total bacteria and anammox bacteria diversity coverage. Salt-adjusted optimum annealing temperature of primer 530F was calculated (47°C) and hence a range of annealing temperatures of 44–49°C was tested. Pyrosequencing data showed that annealing temperature of 45°C yielded the best results in terms of species richness and diversity for all bioreactors analyzed.

scholarly journals Start-Up and Aeration Strategies for a Completely Autotrophic Nitrogen Removal Process in an SBR

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaoling Zhang ◽  
Fan Zhang ◽  
Yanhong Zhao ◽  
Zhengqun Li
Keyword(s):  
Removal Efficiency ◽  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Tap Water ◽  
Granular Sludge ◽  
Anammox Bacteria ◽  
Intermittent Aeration ◽  
Start Up ◽  
Canon Process ◽  
Autotrophic Nitrogen Removal

The start-up and performance of the completely autotrophic nitrogen removal via nitrite (CANON) process were examined in a sequencing batch reactor (SBR) with intermittent aeration. Initially, partial nitrification was established, and then the DO concentration was lowered further, surplus water in the SBR with high nitrite was replaced with tap water, and continuous aeration mode was turned into intermittent aeration mode, while the removal of total nitrogen was still weak. However, the total nitrogen (TN) removal efficiency and nitrogen removal loading reached 83.07% and 0.422 kgN/(m3·d), respectively, 14 days after inoculating 0.15 g of CANON biofilm biomass into the SBR. The aggregates formed in SBR were the mixture of activated sludge and granular sludge; the volume ratio of floc and granular sludge was 7 : 3. DNA analysis showed that Planctomycetes-like anammox bacteria and Nitrosomonas-like aerobic ammonium oxidization bacteria were dominant bacteria in the reactor. The influence of aeration strategies on CANON process was investigated using batch tests. The result showed that the strategy of alternating aeration (1 h) and nonaeration (1 h) was optimum, which can obtain almost the same TN removal efficiency as continuous aeration while reducing the energy consumption, inhibiting the activity of NOB, and enhancing the activity of AAOB.

scholarly journals Achieving fast start-up of anammox process by promoting the growth of anammox bacteria with FeS addition

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Chunzhen Zou ◽  
Beibei Guo ◽  
Xuming Zhuang ◽  
Liying Ren ◽  
Shou-Qing Ni ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Nitrite Reductase ◽  
Removal Rate ◽  
Anammox Bacteria ◽  
Gene Encoding ◽  
Start Up ◽  
Fast Start ◽  
The Difference ◽  
Anammox Process

Abstract The effects of FeS on nitrogen removal performance and microbial community of anammox process were studied. During the start-up period, the removal efficiencies of nitrite and total nitrogen were significantly improved by FeS. The addition of FeS increased the content of iron ions in the reactor and promoted the synthesis of heme c, which was involved in the formation of various enzymes. Compared with the control, the abundance of anammox bacteria in the FeS reactor was increased by 29%, and the expression level of the nirS gene (encoding cd1 type nitrite reductase containing heme) was nearly doubled. The content of nitrite reductase (ammonia-forming) in the community was increased by 26.4%. The difference in functional bacteria and enzyme contents in the microbial community resulted in a difference in nitrogen removal rate (NRR) between the two reactors. High-throughput results indicated that FeS increased the richness and diversity of microbial community and enhanced the metabolic function of the microbial community. The addition of FeS did not change the dominant position of Ca. Kuenenia in both reactors. But the relative abundance of heterotrophic denitrifying bacteria was reduced with FeS, which may be related to the inhibition effect of S2− produced by FeS.

Sign in / Sign up

Export Citation Format

Share Document