Purification, Fermentation Optimization, and Antibacterial Activity of Pyrrole-2-carboxylic Acid Produced by an Endophytic Bacterium, Bacillus cereus ZBE, Isolated from Zanthoxylum bungeanum

2022 ◽  
Author(s):  
Yuxi Yue ◽  
Chong Chen ◽  
Kai Zhong ◽  
Yanping Wu ◽  
Hong Gao
Keyword(s):  
Antibacterial Activity ◽  
Carboxylic Acid ◽  
Bacillus Cereus ◽  
Endophytic Bacterium ◽  
Fermentation Optimization ◽  
Bacterium Bacillus ◽  
Zanthoxylum Bungeanum

scholarly journals Identification and Nematicidal Characterization of Proteases Secreted by Endophytic Bacteria Bacillus cereus BCM2

2020 ◽  
Vol 110 (2) ◽  
pp. 336-344 ◽  
Author(s):  
Haijing Hu ◽  
Yang Gao ◽  
Xia Li ◽  
Shuanglin Chen ◽  
Shuzhen Yan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Bacillus Cereus ◽  
Endophytic Bacteria ◽  
Endophytic Bacterium ◽  
Extracellular Proteins ◽  
Nematicidal Activity ◽  
Alkaline Serine Protease ◽  
Transmission Electron ◽  
Nematode Cuticle ◽  
Bacterium Bacillus

The endophytic bacterium Bacillus cereus BCM2 has shown great potential as a biocontrol organism against Meloidogyne incognita, which causes severe root-knot diseases in crops. In our previous study, the metabolite of BCM2 showed high nematicidal activity against the M. incognita second-stage juveniles. However, the mechanism employed by endophytic bacteria to infect and kill nematodes is still unclear. Here, we investigate both the endophytic bacterial extracellular proteins with nematicidal activity and their mechanism of killing nematodes. The first step was detecting the nematicidal activities of crude proteins. The results show that the nematode mortality rate reached 100% within 72 h, and the crude proteins damaged both the cuticle and eggshell, before finally destroying the targets. This suggests possible proteinaceous pathogeny in BCM2. Throughout the process, the fine-detail changes in the nematode cuticle and the intestinal structure were observed using scanning electron microscopy and transmission electron microscopy. These images show that BCM2 extracellular proteins did not damage the internal organization of the nematode but did severely damage its cuticle, which led to content leakage. From the crude proteins, chitosanase, alkaline serine protease, and neutral protease were purified and identified. The M. incognita–B. cereus BCM2 microenvironment simulation demonstrates that BCM2 adheres to the surface of nematodes and helps the metabolites that were produced by BCM2 to rapidly recognize and kill M. incognita. This relationship between plants, endophytic bacteria, and nematodes offers insight into the biological mechanisms that can be utilized for of nematode management.

scholarly journals Complete Genome Sequence of the Endophytic Bacterium Bacillus cereus PgBE311, Isolated from Panax ginseng

2018 ◽  
Vol 7 (21) ◽  
Author(s):  
Chi Eun Hong ◽  
Jang Uk Kim ◽  
Jung Woo Lee ◽  
Kyong Hwan Bang ◽  
Ick-Hyun Jo
Keyword(s):  
Bacillus Cereus ◽  
Panax Ginseng ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Fungal Pathogens ◽  
Endophytic Bacterium ◽  
Content Type ◽  
Cylindrocarpon Destructans ◽  
Bacterium Bacillus

Bacillus cereus PgBE311, isolated from the root tissue of a 5-year-old Panax ginseng plant, showed activities against the fungal pathogens Cylindrocarpon destructans and Botrytis cinerea. Here, we report the genome sequence of B. cereus PgBE311.

scholarly journals Antioxidant and DNA Damage Protecting Activity of Exopolysaccharides from the Endophytic Bacterium Bacillus cereus SZ1

Molecules ◽  
2016 ◽  
Vol 21 (2) ◽  
pp. 174 ◽  
Author(s):  
Li Zheng ◽  
Tin Zou ◽  
Yan Ma ◽  
Jian Wang ◽  
Yu Zhang
Keyword(s):  
Dna Damage ◽  
Bacillus Cereus ◽  
Endophytic Bacterium ◽  
Bacterium Bacillus

Biotransformation of ginsenoside Rc to Rd by endophytic bacterium Bacillus sp. G9y isolated from Panax quinquefolius

2021 ◽  
Author(s):  
Chen Zhang ◽  
Yanyan Xu ◽  
Mengmeng Gu ◽  
Zhenzhen Liu ◽  
Jingyuan Zhang ◽  
...  
Keyword(s):  
Endophytic Bacterium ◽  
Panax Quinquefolius ◽  
Bacillus Sp ◽  
Bacterium Bacillus ◽  
Ginsenoside Rc

scholarly journals Studies on Quinolone Antibiotics. III. Synthesis and Antibacterial Activity of 5-Amino-7-(2-aminoalkoxy)-1-cyclopropyl-6-fluoro-1, 4-dihydro-4-oxoquinoline-3-carboxylic Acid and Their Derivatives

1991 ◽  
Vol 111 (7) ◽  
pp. 386-392 ◽  
Author(s):  
Toshihiko YOSHIDA ◽  
Yoichi YAMAMOTO ◽  
Noriyuki YAGI ◽  
Yoshie TAKAHASI ◽  
Shingo YASUDA ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Carboxylic Acid ◽  
Quinolone Antibiotics
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